WO2005069725A3 - Fluorescence-based adp detection system - Google Patents

Fluorescence-based adp detection system

Info

Publication number
WO2005069725A3
WO2005069725A3 PCT/IB2005/000290 IB2005000290W WO2005069725A3 WO 2005069725 A3 WO2005069725 A3 WO 2005069725A3 IB 2005000290 W IB2005000290 W IB 2005000290W WO 2005069725 A3 WO2005069725 A3 WO 2005069725A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection system
fluorescence
detecting
adp detection
solution
Prior art date
Application number
PCT/IB2005/000290
Other languages
French (fr)
Other versions
WO2005069725A2 (en
Inventor
Gonzalo Castillo
Richard Leroy Mitchell
Jenny Ann Mulligan
Original Assignee
Mds Pharma Services
Gonzalo Castillo
Richard Leroy Mitchell
Jenny Ann Mulligan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mds Pharma Services, Gonzalo Castillo, Richard Leroy Mitchell, Jenny Ann Mulligan filed Critical Mds Pharma Services
Priority to CA002553218A priority Critical patent/CA2553218A1/en
Priority to JP2006548477A priority patent/JP2007518094A/en
Priority to EP05702434A priority patent/EP1704406A4/en
Publication of WO2005069725A2 publication Critical patent/WO2005069725A2/en
Publication of WO2005069725A3 publication Critical patent/WO2005069725A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices

Abstract

A method and kit for detecting a nucleotide, or differentially detecting one nucleotide in a mixture of at least two nucleotides, in a solution comprising the steps of adding a chelator to the solution and detecting a signal created or altered upon the addition of the chelator using a spectroscopic detection system. A lanthanide may also be added to the solution before the detection of a signal. Preferably, the spectroscopic detection system is a fluorescence-based system.
PCT/IB2005/000290 2004-01-16 2005-01-17 Fluorescence-based adp detection system WO2005069725A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002553218A CA2553218A1 (en) 2004-01-16 2005-01-17 Fluorescence-based adp detection system
JP2006548477A JP2007518094A (en) 2004-01-16 2005-01-17 ADP detection system based on fluorescence
EP05702434A EP1704406A4 (en) 2004-01-16 2005-01-17 Fluorescence-based adp detection system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53673804P 2004-01-16 2004-01-16
US60/536,738 2004-01-16

Publications (2)

Publication Number Publication Date
WO2005069725A2 WO2005069725A2 (en) 2005-08-04
WO2005069725A3 true WO2005069725A3 (en) 2006-04-06

Family

ID=34807038

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2005/000290 WO2005069725A2 (en) 2004-01-16 2005-01-17 Fluorescence-based adp detection system

Country Status (4)

Country Link
EP (1) EP1704406A4 (en)
JP (1) JP2007518094A (en)
CA (1) CA2553218A1 (en)
WO (1) WO2005069725A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4962045A (en) * 1988-05-02 1990-10-09 The Perkin-Elmer Corporation Time-resolved fluorimetric detection of lanthanide labeled nucleotides
US6599711B2 (en) * 2000-12-15 2003-07-29 Lumitech (Uk) Limited Methods and kits for detecting protein kinases

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5792330A (en) 1995-05-31 1998-08-11 Board Of Regents, The University Of Texas System Lanthanide metal cations for concurrent detection and separation in capillary electrophoresis
US5980861A (en) 1996-03-12 1999-11-09 University Of Massachusetts Chelator compositions and methods of synthesis thereof
JP4140929B2 (en) * 1997-01-17 2008-08-27 旭化成ファーマ株式会社 Method for measuring 1,5AG or ADP
CA2514877A1 (en) * 2003-01-30 2004-08-12 Bellbrook Labs, Llc Assay method for group transfer reactions

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4962045A (en) * 1988-05-02 1990-10-09 The Perkin-Elmer Corporation Time-resolved fluorimetric detection of lanthanide labeled nucleotides
US6599711B2 (en) * 2000-12-15 2003-07-29 Lumitech (Uk) Limited Methods and kits for detecting protein kinases

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1704406A4 *

Also Published As

Publication number Publication date
EP1704406A4 (en) 2008-05-28
WO2005069725A2 (en) 2005-08-04
EP1704406A2 (en) 2006-09-27
JP2007518094A (en) 2007-07-05
CA2553218A1 (en) 2005-08-04

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