WO2005082041A3 - Enhanced detection of rna using a panel of truncated gene-specific primers for reverse transcription - Google Patents

Enhanced detection of rna using a panel of truncated gene-specific primers for reverse transcription Download PDF

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Publication number
WO2005082041A3
WO2005082041A3 PCT/US2005/006067 US2005006067W WO2005082041A3 WO 2005082041 A3 WO2005082041 A3 WO 2005082041A3 US 2005006067 W US2005006067 W US 2005006067W WO 2005082041 A3 WO2005082041 A3 WO 2005082041A3
Authority
WO
WIPO (PCT)
Prior art keywords
reverse transcription
specific primers
rna
panel
truncated gene
Prior art date
Application number
PCT/US2005/006067
Other languages
French (fr)
Other versions
WO2005082041A2 (en
Inventor
Michael Mitas
David Coles
Antonette C Allen
Francis Chiafari
Original Assignee
Brt Lab Inc
Michael Mitas
David Coles
Antonette C Allen
Francis Chiafari
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brt Lab Inc, Michael Mitas, David Coles, Antonette C Allen, Francis Chiafari filed Critical Brt Lab Inc
Publication of WO2005082041A2 publication Critical patent/WO2005082041A2/en
Publication of WO2005082041A3 publication Critical patent/WO2005082041A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Abstract

The present invention provides truncated gene-specific primers in panels that can be used during the reverse transcription step of RT-PCR to increase signal detection of cancer gene markers in a tissue sample. Also provided are forward and reverse primers for RT-PCR. Methods of using the primers are also provided.
PCT/US2005/006067 2004-02-27 2005-02-28 Enhanced detection of rna using a panel of truncated gene-specific primers for reverse transcription WO2005082041A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US54855204P 2004-02-27 2004-02-27
US60/548,552 2004-02-27

Publications (2)

Publication Number Publication Date
WO2005082041A2 WO2005082041A2 (en) 2005-09-09
WO2005082041A3 true WO2005082041A3 (en) 2009-06-11

Family

ID=34911005

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2005/006292 WO2005084254A2 (en) 2004-02-27 2005-02-25 Enhanced detection of rna using a panel of truncated gene-specific primers for reverse transcription
PCT/US2005/006067 WO2005082041A2 (en) 2004-02-27 2005-02-28 Enhanced detection of rna using a panel of truncated gene-specific primers for reverse transcription

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2005/006292 WO2005084254A2 (en) 2004-02-27 2005-02-25 Enhanced detection of rna using a panel of truncated gene-specific primers for reverse transcription

Country Status (2)

Country Link
US (1) US7981616B2 (en)
WO (2) WO2005084254A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006138716A2 (en) * 2005-06-17 2006-12-28 Brt Laboratories, Inc. Thermodynamically defined gene specific primers
EP2744916A4 (en) 2011-07-13 2015-06-17 Primeradx Inc Multimodal methods for simultaneous detection and quantification of multiple nucleic acids in a sample
US20140005058A1 (en) * 2012-06-28 2014-01-02 Caldera Health Ltd. Methods and materials for the diagnosis of prostate cancers
JP7184373B2 (en) * 2020-01-29 2022-12-06 学校法人九州文化学園 Ex vivo test method for intestinal tumor

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Also Published As

Publication number Publication date
WO2005084254A3 (en) 2006-08-10
US7981616B2 (en) 2011-07-19
WO2005084254A2 (en) 2005-09-15
US20080233563A1 (en) 2008-09-25
WO2005082041A2 (en) 2005-09-09

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