WO2005084254A3 - Enhanced detection of rna using a panel of truncated gene-specific primers for reverse transcription - Google Patents

Enhanced detection of rna using a panel of truncated gene-specific primers for reverse transcription Download PDF

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Publication number
WO2005084254A3
WO2005084254A3 PCT/US2005/006292 US2005006292W WO2005084254A3 WO 2005084254 A3 WO2005084254 A3 WO 2005084254A3 US 2005006292 W US2005006292 W US 2005006292W WO 2005084254 A3 WO2005084254 A3 WO 2005084254A3
Authority
WO
WIPO (PCT)
Prior art keywords
reverse transcription
specific primers
rna
panel
truncated gene
Prior art date
Application number
PCT/US2005/006292
Other languages
French (fr)
Other versions
WO2005084254A2 (en
Inventor
Michael Mitas
David J Cole
William E Gillanders
Kaidi Mikhitarian
Original Assignee
Musc Found For Res Dev
Michael Mitas
David J Cole
William E Gillanders
Kaidi Mikhitarian
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Musc Found For Res Dev, Michael Mitas, David J Cole, William E Gillanders, Kaidi Mikhitarian filed Critical Musc Found For Res Dev
Priority to US10/590,891 priority Critical patent/US7981616B2/en
Publication of WO2005084254A2 publication Critical patent/WO2005084254A2/en
Publication of WO2005084254A3 publication Critical patent/WO2005084254A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Abstract

The present invention provides truncated gene-specific primers in panels that can be used during the reverse transcription step of RT-PCR to increase signal detection of cancer gene markers in a tissue sample. Also provided are forward and reverse primers for RT­-PCR. Methods of using the primers are also provided.
PCT/US2005/006292 2004-02-27 2005-02-25 Enhanced detection of rna using a panel of truncated gene-specific primers for reverse transcription WO2005084254A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US10/590,891 US7981616B2 (en) 2004-02-27 2005-02-25 Enhanced detection of RNA using a panel of truncated gene-specific primers for reverse transcription

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US54855204P 2004-02-27 2004-02-27
US60/548,552 2004-02-27

Publications (2)

Publication Number Publication Date
WO2005084254A2 WO2005084254A2 (en) 2005-09-15
WO2005084254A3 true WO2005084254A3 (en) 2006-08-10

Family

ID=34911005

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2005/006292 WO2005084254A2 (en) 2004-02-27 2005-02-25 Enhanced detection of rna using a panel of truncated gene-specific primers for reverse transcription
PCT/US2005/006067 WO2005082041A2 (en) 2004-02-27 2005-02-28 Enhanced detection of rna using a panel of truncated gene-specific primers for reverse transcription

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2005/006067 WO2005082041A2 (en) 2004-02-27 2005-02-28 Enhanced detection of rna using a panel of truncated gene-specific primers for reverse transcription

Country Status (2)

Country Link
US (1) US7981616B2 (en)
WO (2) WO2005084254A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006138716A2 (en) * 2005-06-17 2006-12-28 Brt Laboratories, Inc. Thermodynamically defined gene specific primers
WO2013010074A1 (en) 2011-07-13 2013-01-17 Primeradx, Inc. Multimodal methods for simultaneous detection and quantification of multiple nucleic acids in a sample
CA2877864C (en) * 2012-06-28 2019-05-14 James Douglas Watson Targeted rna-seq methods and materials for the diagnosis of prostate cancer
JP7184373B2 (en) * 2020-01-29 2022-12-06 学校法人九州文化学園 Ex vivo test method for intestinal tumor

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5801016A (en) * 1995-03-29 1998-09-01 Japan Tobacco Inc. DNA fragment, recombinant vector containing the same and method for expressing foreign genes using the same

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US616952A (en) * 1899-01-03 Detachable hinge
US5274087A (en) * 1986-08-13 1993-12-28 Molecular Diagnostics, Inc. cDNA coding for carcinoembryonic antigen (CEA)
US6416952B1 (en) 1989-06-07 2002-07-09 Affymetrix, Inc. Photolithographic and other means for manufacturing arrays
US6406844B1 (en) 1989-06-07 2002-06-18 Affymetrix, Inc. Very large scale immobilized polymer synthesis
US6040138A (en) * 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US6346413B1 (en) * 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
EP0519338B1 (en) * 1991-06-20 1996-08-28 F. Hoffmann-La Roche Ag Improved methods for nucleic acid amplification
US6309823B1 (en) * 1993-10-26 2001-10-30 Affymetrix, Inc. Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same
EP1350514A3 (en) 1993-10-29 2004-07-07 The Brigham And Women's Hospital, Inc. Therapeutic use of cis-element decoys in vivo
US5773213A (en) * 1994-06-06 1998-06-30 Brigham & Women's Hospital Method for conducting sequential nucleic acid hybridization steps
DE69632870T2 (en) * 1995-03-17 2005-07-14 John Wayne Cancer Institute, Santa Monica DETECTION OF MELANOMA MEASURING TASTES USING MULTILARMARKER TEST
US5668267A (en) * 1995-05-31 1997-09-16 Washington University Polynucleotides encoding mammaglobin, a mammary-specific breast cancer protein
CN1151269C (en) * 1995-06-06 2004-05-26 扬·维吉 Method of and apparatus for diagnostic DNA testing
US5882856A (en) * 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US5837442A (en) * 1995-11-29 1998-11-17 Roche Molecular Systems, Inc. Oligonucleotide primers for amplifying HCV nucleic acid
AUPO188396A0 (en) 1996-08-26 1996-09-19 Insearch Limited Detection of protozoan parasites
US5958349A (en) * 1997-02-28 1999-09-28 Cepheid Reaction vessel for heat-exchanging chemical processes
US5843721A (en) * 1997-07-03 1998-12-01 Tularik Inc. Nucleic acids encoding human NIK protein
US6440725B1 (en) 1997-12-24 2002-08-27 Cepheid Integrated fluid manipulation cartridge
GB9719044D0 (en) 1997-09-08 1997-11-12 Inst Of Ophthalmology Assay
US6660228B1 (en) 1998-03-02 2003-12-09 Cepheid Apparatus for performing heat-exchanging, chemical reactions
US6037129A (en) * 1998-05-28 2000-03-14 Medical University Of South Carolina Multi-marker RT-PCR panel for detecting metastatic breast cancer
US6306643B1 (en) * 1998-08-24 2001-10-23 Affymetrix, Inc. Methods of using an array of pooled probes in genetic analysis
US6245517B1 (en) * 1998-09-29 2001-06-12 The United States Of America As Represented By The Department Of Health And Human Services Ratio-based decisions and the quantitative analysis of cDNA micro-array images
US6431476B1 (en) 1999-12-21 2002-08-13 Cepheid Apparatus and method for rapid ultrasonic disruption of cells or viruses
EP1146869A2 (en) 1999-01-27 2001-10-24 University Of South Florida Inhibition of stat3 signal transduction for human cancer therapy
US6251601B1 (en) * 1999-02-02 2001-06-26 Vysis, Inc. Simultaneous measurement of gene expression and genomic abnormalities using nucleic acid microarrays
US6300069B1 (en) 1999-05-03 2001-10-09 Qiagen Gmbh Generation and amplification of nucleic acids from ribonucleic acids
ATE278771T1 (en) 1999-05-28 2004-10-15 Cepheid APPARATUS AND METHOD FOR ANALYZING LIQUID SAMPLES
ATE333938T1 (en) 1999-05-28 2006-08-15 Cepheid CARTRIDGE FOR PERFORMING A CHEMICAL REACTION
SE9902410D0 (en) 1999-06-24 1999-06-24 Cavidi Tech Ab Reverse transcriptase assay kit, use thereof and method for analysis of RT activity in biological samples
US6403037B1 (en) 2000-02-04 2002-06-11 Cepheid Reaction vessel and temperature control system
US6783934B1 (en) 2000-05-01 2004-08-31 Cepheid, Inc. Methods for quantitative analysis of nucleic acid amplification reaction
US8048386B2 (en) 2002-02-25 2011-11-01 Cepheid Fluid processing and control
US6374684B1 (en) 2000-08-25 2002-04-23 Cepheid Fluid control and processing system
US6312929B1 (en) 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
US7101663B2 (en) 2001-03-02 2006-09-05 University of Pittsburgh—of the Commonwealth System of Higher Education PCR method
JPWO2003055973A1 (en) 2001-12-26 2005-05-12 オリンパス株式会社 Reaction vessel and reaction vessel holding mechanism
US6819027B2 (en) 2002-03-04 2004-11-16 Cepheid Method and apparatus for controlling ultrasonic transducer
US20040101859A1 (en) 2002-11-25 2004-05-27 Cepheid Compositions, methods and kits for polynucleotide amplification reactions and microfluidic devices
US7824857B2 (en) 2003-12-02 2010-11-02 Musc Foundation For Research Development Methods and compositions for diagnosing epithelial cell cancer

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5801016A (en) * 1995-03-29 1998-09-01 Japan Tobacco Inc. DNA fragment, recombinant vector containing the same and method for expressing foreign genes using the same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK [online] 27 October 1999 (1999-10-27), CZYGAN ET AL.: "Borna disease virus in human brains with a rare form of hippocampal degeneration but not in brains of patients with common neuropsychiatre disorders", Database accession no. (AJ246858.1) *
INFECT. DIS., vol. 180, no. 5, 1999, pages 1695 - 1699 *

Also Published As

Publication number Publication date
WO2005082041A2 (en) 2005-09-09
WO2005082041A3 (en) 2009-06-11
WO2005084254A2 (en) 2005-09-15
US20080233563A1 (en) 2008-09-25
US7981616B2 (en) 2011-07-19

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