WO2005097980A3 - New protocols for making hepatocytes from embryonic stem cells - Google Patents

New protocols for making hepatocytes from embryonic stem cells Download PDF

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Publication number
WO2005097980A3
WO2005097980A3 PCT/US2005/009972 US2005009972W WO2005097980A3 WO 2005097980 A3 WO2005097980 A3 WO 2005097980A3 US 2005009972 W US2005009972 W US 2005009972W WO 2005097980 A3 WO2005097980 A3 WO 2005097980A3
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Prior art keywords
cells
stem cells
hepatocytes
making
embryonic stem
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PCT/US2005/009972
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French (fr)
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WO2005097980A2 (en
Inventor
Ramkumar Mandalam
Saadia Faouzi
Isabelle Nadeau-Demers
Kristina Bonham
Namitha Rao
Melissa K Carpentier
Lakshmi Rambhatla
Choy-Pik Chiu
Original Assignee
Geron Corp
Ramkumar Mandalam
Saadia Faouzi
Isabelle Nadeau-Demers
Kristina Bonham
Namitha Rao
Melissa K Carpentier
Lakshmi Rambhatla
Choy-Pik Chiu
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Application filed by Geron Corp, Ramkumar Mandalam, Saadia Faouzi, Isabelle Nadeau-Demers, Kristina Bonham, Namitha Rao, Melissa K Carpentier, Lakshmi Rambhatla, Choy-Pik Chiu filed Critical Geron Corp
Publication of WO2005097980A2 publication Critical patent/WO2005097980A2/en
Publication of WO2005097980A3 publication Critical patent/WO2005097980A3/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
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    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Abstract

This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.
PCT/US2005/009972 2004-03-26 2005-03-24 New protocols for making hepatocytes from embryonic stem cells WO2005097980A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55683004P 2004-03-26 2004-03-26
US60/556,830 2004-03-26

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WO2005097980A2 WO2005097980A2 (en) 2005-10-20
WO2005097980A3 true WO2005097980A3 (en) 2007-12-27

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