WO2005098430A3 - Image based quantitation of molecular translocation - Google Patents

Image based quantitation of molecular translocation Download PDF

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Publication number
WO2005098430A3
WO2005098430A3 PCT/US2005/008866 US2005008866W WO2005098430A3 WO 2005098430 A3 WO2005098430 A3 WO 2005098430A3 US 2005008866 W US2005008866 W US 2005008866W WO 2005098430 A3 WO2005098430 A3 WO 2005098430A3
Authority
WO
WIPO (PCT)
Prior art keywords
compartment
molecular
cell
marker
translocation
Prior art date
Application number
PCT/US2005/008866
Other languages
French (fr)
Other versions
WO2005098430A2 (en
Inventor
Thaddeus C George
David A Basiji
Keith Frost
Brian E Hall
William E Ortyn
Michael J Seo
Original Assignee
Amnis Corp
Thaddeus C George
David A Basiji
Keith Frost
Brian E Hall
William E Ortyn
Michael J Seo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amnis Corp, Thaddeus C George, David A Basiji, Keith Frost, Brian E Hall, William E Ortyn, Michael J Seo filed Critical Amnis Corp
Priority to US10/593,018 priority Critical patent/US8150136B2/en
Priority to CA002598602A priority patent/CA2598602A1/en
Priority to EP05760334A priority patent/EP1800124B1/en
Priority to AT05760334T priority patent/ATE538138T1/en
Publication of WO2005098430A2 publication Critical patent/WO2005098430A2/en
Publication of WO2005098430A3 publication Critical patent/WO2005098430A3/en
Priority to US13/436,371 priority patent/US9528989B2/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells

Abstract

The use of a multi-spectral imaging system, cell compartment markers, and molecular probes in a method for measuring movement of molecules within a cell by correlation analysis is provided, including measuring molecular movement to a particular compartment in adherent and non-adherent cells, e.g. in response to biological stimuli. A compartment in the cell is defined by the image of a specific compartment marker, e.g., a nuclear fluorescent stain. Molecule location is provided by a probe labeled with a different fluorochrome. A mask is generated based on the compartmental marker, and a correlation measurement is made between the locations of the molecular probe and the compartment marker. The correlation value between the regions defined by the compartment mask and molecular probe gives a quantitative measurement of the translocation of the molecule. The use of only a single masking function simplifies measurement of molecular translocation within a cell.
PCT/US2005/008866 2004-03-16 2005-03-16 Image based quantitation of molecular translocation WO2005098430A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US10/593,018 US8150136B2 (en) 2004-03-16 2005-03-16 Image based quantitation of molecular translocation
CA002598602A CA2598602A1 (en) 2004-03-16 2005-03-16 Image based quantitation of molecular translocation
EP05760334A EP1800124B1 (en) 2004-03-16 2005-03-16 Image based quantitation of molecular translocation
AT05760334T ATE538138T1 (en) 2004-03-16 2005-03-16 IMAGING-BASED QUANTIFICATION OF MOLECULAR TRANSLOCATION
US13/436,371 US9528989B2 (en) 2004-03-16 2012-03-30 Image-based quantitation of molecular translocation

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US55348404P 2004-03-16 2004-03-16
US60/553,484 2004-03-16
US57287704P 2004-05-19 2004-05-19
US60/572,877 2004-05-19

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10/593,018 A-371-Of-International US8150136B2 (en) 2004-03-16 2005-03-16 Image based quantitation of molecular translocation
US13/436,371 Continuation US9528989B2 (en) 2004-03-16 2012-03-30 Image-based quantitation of molecular translocation

Publications (2)

Publication Number Publication Date
WO2005098430A2 WO2005098430A2 (en) 2005-10-20
WO2005098430A3 true WO2005098430A3 (en) 2005-12-15

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/008866 WO2005098430A2 (en) 2004-03-16 2005-03-16 Image based quantitation of molecular translocation

Country Status (5)

Country Link
US (2) US8150136B2 (en)
EP (1) EP1800124B1 (en)
AT (1) ATE538138T1 (en)
CA (1) CA2598602A1 (en)
WO (1) WO2005098430A2 (en)

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