WO2005121318A3 - Neural stem cells - Google Patents

Neural stem cells Download PDF

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Publication number
WO2005121318A3
WO2005121318A3 PCT/GB2005/002289 GB2005002289W WO2005121318A3 WO 2005121318 A3 WO2005121318 A3 WO 2005121318A3 GB 2005002289 W GB2005002289 W GB 2005002289W WO 2005121318 A3 WO2005121318 A3 WO 2005121318A3
Authority
WO
WIPO (PCT)
Prior art keywords
neural stem
stem cells
activator
receptor
signalling pathway
Prior art date
Application number
PCT/GB2005/002289
Other languages
French (fr)
Other versions
WO2005121318A2 (en
WO2005121318A8 (en
Inventor
Luciano Conti
Steven Michael Pollard
Austin Gerard Smith
Qi-Long Ying
Original Assignee
Univ Edinburgh
Luciano Conti
Steven Michael Pollard
Austin Gerard Smith
Qi-Long Ying
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0412887.2A external-priority patent/GB0412887D0/en
Priority claimed from GBGB0505510.8A external-priority patent/GB0505510D0/en
Priority to PL05752405T priority Critical patent/PL1758986T3/en
Priority to CA2569978A priority patent/CA2569978C/en
Priority to CN2005800250966A priority patent/CN101124319B/en
Priority to ES05752405.0T priority patent/ES2557772T3/en
Priority to JP2007526548A priority patent/JP2008501356A/en
Priority to NZ551832A priority patent/NZ551832A/en
Application filed by Univ Edinburgh, Luciano Conti, Steven Michael Pollard, Austin Gerard Smith, Qi-Long Ying filed Critical Univ Edinburgh
Priority to EP15187477.3A priority patent/EP3000877B1/en
Priority to DK05752405.0T priority patent/DK1758986T3/en
Priority to AU2005252434A priority patent/AU2005252434B2/en
Priority to EP05752405.0A priority patent/EP1758986B1/en
Publication of WO2005121318A2 publication Critical patent/WO2005121318A2/en
Publication of WO2005121318A3 publication Critical patent/WO2005121318A3/en
Priority to IL179919A priority patent/IL179919A/en
Publication of WO2005121318A8 publication Critical patent/WO2005121318A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/02—Drugs for disorders of the nervous system for peripheral neuropathies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

Abstract

A homogenous, symmetrically dividing population of adherent neural stem cells is obtained from ES cells or foetal or adult brain isolates, using an activator of a signalling pathway downstream of a receptor of the EGF receptor family, optionally in combination with an activator of a signalling pathway downstream of an FGF receptor. The neural stem cell population is highly pure and retains the ability to differentiate into neurons after in excess of 100 passages.
PCT/GB2005/002289 2004-06-09 2005-06-09 Neural stem cells WO2005121318A2 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
AU2005252434A AU2005252434B2 (en) 2004-06-09 2005-06-09 Neural stem cells
EP05752405.0A EP1758986B1 (en) 2004-06-09 2005-06-09 Neural stem cells
EP15187477.3A EP3000877B1 (en) 2004-06-09 2005-06-09 Neural stem cells
CN2005800250966A CN101124319B (en) 2004-06-09 2005-06-09 Neural stem cells
ES05752405.0T ES2557772T3 (en) 2004-06-09 2005-06-09 Neural stem cells
JP2007526548A JP2008501356A (en) 2004-06-09 2005-06-09 Neural stem cells
NZ551832A NZ551832A (en) 2004-06-09 2005-06-09 Neural stem cells
PL05752405T PL1758986T3 (en) 2004-06-09 2005-06-09 Neural stem cells
CA2569978A CA2569978C (en) 2004-06-09 2005-06-09 Neural stem cells
DK05752405.0T DK1758986T3 (en) 2004-06-09 2005-06-09 Neural stem cells
IL179919A IL179919A (en) 2004-06-09 2006-12-07 Neural stem cells, compositions comprising the same and methods of obtaining the same

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB0412887.2 2004-06-09
GBGB0412887.2A GB0412887D0 (en) 2004-06-09 2004-06-09 Control of neural stem cells
GBGB0503044.0A GB0503044D0 (en) 2004-06-09 2005-02-14 Neural stem cells
GB0503044.0 2005-02-14
GB0505510.8 2005-03-17
GBGB0505510.8A GB0505510D0 (en) 2004-06-09 2005-03-17 Neural stem cells

Publications (3)

Publication Number Publication Date
WO2005121318A2 WO2005121318A2 (en) 2005-12-22
WO2005121318A3 true WO2005121318A3 (en) 2006-03-23
WO2005121318A8 WO2005121318A8 (en) 2014-08-28

Family

ID=34864834

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2005/002289 WO2005121318A2 (en) 2004-06-09 2005-06-09 Neural stem cells

Country Status (13)

Country Link
US (2) US8785187B2 (en)
EP (2) EP3000877B1 (en)
JP (2) JP2008501356A (en)
CN (1) CN103146649B (en)
AU (1) AU2005252434B2 (en)
CA (1) CA2569978C (en)
DK (1) DK1758986T3 (en)
ES (1) ES2557772T3 (en)
GB (1) GB2414995B (en)
IL (1) IL179919A (en)
NZ (1) NZ551832A (en)
PL (1) PL1758986T3 (en)
WO (1) WO2005121318A2 (en)

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