WO2006014576A3 - Fluorescent lifetime biological detection and imaging using water-stable semiconductor nanocrystals - Google Patents

Fluorescent lifetime biological detection and imaging using water-stable semiconductor nanocrystals Download PDF

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Publication number
WO2006014576A3
WO2006014576A3 PCT/US2005/024211 US2005024211W WO2006014576A3 WO 2006014576 A3 WO2006014576 A3 WO 2006014576A3 US 2005024211 W US2005024211 W US 2005024211W WO 2006014576 A3 WO2006014576 A3 WO 2006014576A3
Authority
WO
WIPO (PCT)
Prior art keywords
water
imaging
semiconductor nanocrystals
stable semiconductor
biological detection
Prior art date
Application number
PCT/US2005/024211
Other languages
French (fr)
Other versions
WO2006014576A2 (en
Inventor
Wei Liu
Zuoyan Peng
Michael Locascio
Original Assignee
Evident Technologies
Wei Liu
Zuoyan Peng
Michael Locascio
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evident Technologies, Wei Liu, Zuoyan Peng, Michael Locascio filed Critical Evident Technologies
Priority to CA002573150A priority Critical patent/CA2573150A1/en
Priority to EP05769351A priority patent/EP1784502A4/en
Publication of WO2006014576A2 publication Critical patent/WO2006014576A2/en
Publication of WO2006014576A3 publication Critical patent/WO2006014576A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nanotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Materials Engineering (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Composite Materials (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

A method of detecting target biological molecules in a target sample is provided. The method includes using spectral, temporal, and polarization characteristics of emitted light from water-stable semiconductor nanocrystal complexes to detect the presence of target biological molecules in a target sample.
PCT/US2005/024211 2002-10-23 2005-07-08 Fluorescent lifetime biological detection and imaging using water-stable semiconductor nanocrystals WO2006014576A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002573150A CA2573150A1 (en) 2004-07-08 2005-07-08 Fluorescent lifetime biological detection and imaging using water-stable semiconductor nanocrystals
EP05769351A EP1784502A4 (en) 2004-07-08 2005-07-08 Fluorescent lifetime biological detection and imaging using water-stable semiconductor nanocrystals

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US42053402P 2002-10-23 2002-10-23
US48524303P 2003-07-08 2003-07-08
US10/885,867 US7192780B2 (en) 2002-10-23 2004-07-08 Fluorescent lifetime biological detection and imaging using water-stable semiconductor nanocrystals
US10/885,867 2004-07-08

Publications (2)

Publication Number Publication Date
WO2006014576A2 WO2006014576A2 (en) 2006-02-09
WO2006014576A3 true WO2006014576A3 (en) 2006-09-28

Family

ID=35787664

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/024211 WO2006014576A2 (en) 2002-10-23 2005-07-08 Fluorescent lifetime biological detection and imaging using water-stable semiconductor nanocrystals

Country Status (2)

Country Link
US (1) US7192780B2 (en)
WO (1) WO2006014576A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7746681B2 (en) * 2005-01-07 2010-06-29 Invisage Technologies, Inc. Methods of making quantum dot films
US7742322B2 (en) * 2005-01-07 2010-06-22 Invisage Technologies, Inc. Electronic and optoelectronic devices with quantum dot films
US7773404B2 (en) * 2005-01-07 2010-08-10 Invisage Technologies, Inc. Quantum dot optical devices with enhanced gain and sensitivity and methods of making same
US8003010B2 (en) * 2004-05-10 2011-08-23 Samsung Electronics Co., Ltd. Water-stable III-V semiconductor nanocrystal complexes and methods of making same
CA2519608A1 (en) * 2005-01-07 2006-07-07 Edward Sargent Quantum dot-polymer nanocomposite photodetectors and photovoltaics
EP1851546A4 (en) * 2005-02-01 2009-02-04 Evident Technologies Semiconductor nanocrystal complexes and methods of detecting molecular interactions using same
EP2230264B1 (en) * 2006-06-29 2019-10-09 Life Technologies AS Particles containing multi-block polymers
US8021517B2 (en) * 2008-02-28 2011-09-20 Honeywell Asca Inc. Use of fluorescent nanoparticles to make on-line measurements of cross-web and machine-direction component and property variations in paper and continuous web products
DE102008012635A1 (en) * 2008-03-05 2009-09-10 Carl Zeiss Microlmaging Gmbh Method and arrangement for time-resolved spectroscopy
US7858953B2 (en) * 2008-05-23 2010-12-28 Honeywell Asca Inc. Use of fluorescent nanoparticles to measure individual layer thicknesses or composition in multi-layer films and to calibrate secondary measurement devices
TWI365185B (en) * 2008-07-24 2012-06-01 Lilly Co Eli Amidophenoxyindazoles useful as inhibitors of c-met
US9346998B2 (en) 2009-04-23 2016-05-24 The University Of Chicago Materials and methods for the preparation of nanocomposites
DE102011002080B4 (en) * 2011-04-15 2016-05-04 Lre Medical Gmbh Apparatus and method for determining the concentration of fluorophores in a sample

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6274323B1 (en) * 1999-05-07 2001-08-14 Quantum Dot Corporation Method of detecting an analyte in a sample using semiconductor nanocrystals as a detectable label
US6306610B1 (en) * 1998-09-18 2001-10-23 Massachusetts Institute Of Technology Biological applications of quantum dots

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6251303B1 (en) * 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
US6617583B1 (en) * 1998-09-18 2003-09-09 Massachusetts Institute Of Technology Inventory control
US6426513B1 (en) * 1998-09-18 2002-07-30 Massachusetts Institute Of Technology Water-soluble thiol-capped nanocrystals
US6872450B2 (en) 2002-10-23 2005-03-29 Evident Technologies Water-stable photoluminescent semiconductor nanocrystal complexes and method of making same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306610B1 (en) * 1998-09-18 2001-10-23 Massachusetts Institute Of Technology Biological applications of quantum dots
US6274323B1 (en) * 1999-05-07 2001-08-14 Quantum Dot Corporation Method of detecting an analyte in a sample using semiconductor nanocrystals as a detectable label

Also Published As

Publication number Publication date
US7192780B2 (en) 2007-03-20
WO2006014576A2 (en) 2006-02-09
US20050251027A1 (en) 2005-11-10

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