WO2006020255A2 - Apparatus and methods for treating tissue using passive injection systems - Google Patents
Apparatus and methods for treating tissue using passive injection systems Download PDFInfo
- Publication number
- WO2006020255A2 WO2006020255A2 PCT/US2005/025469 US2005025469W WO2006020255A2 WO 2006020255 A2 WO2006020255 A2 WO 2006020255A2 US 2005025469 W US2005025469 W US 2005025469W WO 2006020255 A2 WO2006020255 A2 WO 2006020255A2
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- WO
- WIPO (PCT)
- Prior art keywords
- needle
- bioactive agent
- tissue
- lumen
- damaged region
- Prior art date
Links
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/34—Trocars; Puncturing needles
- A61B17/3478—Endoscopic needles, e.g. for infusion
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/00234—Surgical instruments, devices or methods, e.g. tourniquets for minimally invasive surgery
- A61B2017/00238—Type of minimally invasive operation
- A61B2017/00243—Type of minimally invasive operation cardiac
- A61B2017/00247—Making holes in the wall of the heart, e.g. laser Myocardial revascularization
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B2017/00367—Details of actuation of instruments, e.g. relations between pushing buttons, or the like, and activation of the tool, working tip, or the like
- A61B2017/00398—Details of actuation of instruments, e.g. relations between pushing buttons, or the like, and activation of the tool, working tip, or the like using powered actuators, e.g. stepper motors, solenoids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B2017/00831—Material properties
- A61B2017/00867—Material properties shape memory effect
- A61B2017/00871—Material properties shape memory effect polymeric
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/34—Trocars; Puncturing needles
- A61B17/3403—Needle locating or guiding means
- A61B2017/3405—Needle locating or guiding means using mechanical guide means
- A61B2017/3411—Needle locating or guiding means using mechanical guide means with a plurality of holes, e.g. holes in matrix arrangement
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/34—Trocars; Puncturing needles
- A61B2017/348—Means for supporting the trocar against the body or retaining the trocar inside the body
- A61B2017/3482—Means for supporting the trocar against the body or retaining the trocar inside the body inside
- A61B2017/3484—Anchoring means, e.g. spreading-out umbrella-like structure
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B18/00—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body
- A61B2018/00315—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body for treatment of particular body parts
- A61B2018/00345—Vascular system
- A61B2018/00351—Heart
- A61B2018/00392—Transmyocardial revascularisation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/178—Syringes
- A61M5/31—Details
- A61M5/32—Needles; Details of needles pertaining to their connection with syringe or hub; Accessories for bringing the needle into, or holding the needle on, the body; Devices for protection of needles
- A61M5/3287—Accessories for bringing the needle into the body; Automatic needle insertion
Definitions
- the present invention relates to apparatus and method for treating an injured spinal cord and other injured tissue using passive injection systems that reduce barotrauma to the injected material and collateral damage to the host tissue.
- Spinal cord injuries may arise from car accidents, violent crimes, falls and sports injuries.
- Spinal cord injury is a major neurological problem since most damage resulting from the injury is irreversible. Injured nerves fibers do not normally regenerate with resulting loss of nerve cell communication, leading to paralysis and loss of sensation.
- spinal cord severance a new glial basal lamina forms to cover the exposed surface of the cord end regions.
- the glial cells also secrete barrier molecules that are difficult to penetrate, further suppressing reestablishment of nerve interconnections.
- the spinal cord tissue bordering the severed region becomes necrotic, detaches from the spinal cord, and develops irregular cavities.
- stem cells Most tissue in the human body originates from undifferentiated cells known as stem cells. These fundamental building blocks differentiate into specific target parenchymal tissue based on hormonal and other local signals. Scientific evidence suggests that stem cells injected into a target tissue will differentiate into a cell line specific to the host tissue. This capability is of particular interest in treating conditions involving organs, such as the spinal cord, heart and brain that cannot regenerate.
- Recent developments in stem cell research suggest adult stem cells can be harvested from the bone marrow and other tissues. Many such "cell lines" have been generated and are undergoing clinical evaluation. If successful, this work will obviate the moral and ethical dilemma of utilizing tissue from embryos for research.
- Pressurized direct injection of certain bioactive agents, such as stem cells, is expected to inflict physical damage to the cell membranes due to fluid turbulence and pressure fluctuations (referred to herein as "barotrauma") during the injection process.
- the damage may include lysis of the cells or injury to the cells that may significantly reduce the yield of viable cells delivered at the injection site and/or trauma to the target tissue. Forceful injection of any material into tissue also may disrupt the delicate intercellular matrix, thereby causing target tissue cellular injury.
- atraumatic deployment means deployment of the stem cells without generating turbulent fluid motion that inflicts physical damage to the stem cells, e.g., due to high shearing stresses or pressure fluctuations.
- the bioactive agent preferably is delivered in a solution comprising nutrients to foster stem cell survival after implantation, and one or more drugs or hormones to suppress inflammatory response, etc.
- the bioactive agent is directly deployed in a needle track formed in a target tissue mass following formation of the needle track.
- the bioactive agent is not subject to barotrauma during delivery, nor does forceful impingement of the injectate during delivery disrupt the pre-existing intercellular matrix.
- Deployment of stem cells preferably is accomplished using needle arrangements that avoid impingement of the stem cells against target tissue at high velocity by employing low-pressure injection, capillary action or electrostatic forces to eject the stem cells out of the needle during needle withdrawal.
- a column of stem cells may ⁇ be advanced simultaneously with a needle during needle insertion, and then held stationary while retracting the needle.
- the needle comprises an electroactive polymer that contracts along its length to expel the stem cells into the needle track.
- electromotive forces are employed to deposit the stem cells into the needle track.
- a grid may be positioned over the injured portion of the spinal cord to guide injections of the bioactive agent.
- the apparatus and methods of the present invention advantageously may be employed wherever it is desired to promote tissue regeneration, such as in the heart, kidney, liver, brain and other organs and muscles.
- FIGS. 1A-1C are views depicting previously known methods of injecting drugs and other bioactive agents into a tissue mass
- FIGS. 2A-2C are views depicting a method of injecting drugs and other bioactive agents into a tissue mass in accordance with the principles of the present invention
- FIGS. 3A and 3B are views depicting apparatus of the present invention for injecting drugs and other bioactive agents into a tissue mass at multiple sites simultaneousIy
- FIGS. 4A and 4B are, respectively, a side view, partly in section, and isolation view of the internal components of apparatus of the present invention
- FIGS. 5A-5C depict operation of the apparatus of FIGS. 4;
- FIGS. 6A and 6B are cross-sectional views of apparatus and methods of the present invention for injecting a bioactive agent into an injured spinal cord;
- FIGS. 7A and 7B are cross-sectional views of another embodiment of the apparatus of the present invention.
- FIGS. 8A and 8B are cross-sectional views of a further alternative embodiment of apparatus of the present invention.
- FIG. IA illustrates a previously known injection needle 10 being brought into approximation with tissue mass T. Once the tip of needle 10 is inserted into the tissue, as shown in FIG. IB, bioactive agent B, which may comprise stem cells, is injected into the tissue mass.
- bioactive agent B which may comprise stem cells
- pressurized injection of a bioactive agent may have a substantial detrimental effect both on the agent delivered and the tissue to be treated.
- pressurized injection causes the injectate stream to impinge violently against the tissue as it leaves the tip of the injection needle.
- the injectate stream is turbulent, and may experience rapid localized pressure fluctuations. These effects may damage the bioactive agent, particularly where the agent comprises stem cells, by rupturing the cell membrane or injuring the cellular components.
- FIGS. 2A to 2C apparatus and methods of the present invention are described that overcome the drawbacks of previously known systems for delivering fragile bioactive agents, such as stem cells.
- needle 20 is first, approximated to spinal cord tissue mass T.
- FIG. 2B needle 20 is shown inserted into the tissue mass.
- FIG. 2C as needle 20 is withdrawn from the tissue mass, bioactive agent B is delivered from the tip of the needle and deposited in the needle track.
- the bioactive agent is injected into the tissue under little pressure and with substantially less turbulence and localized pressure fluctuation than in previously known injection systems. Also, the bioactive agent will not damage the tissue mass by splitting the tissue along naturally-occurring striations. These benefits of atraumatic injection may be particularly advantageous in the repair of an injured or severed spinal cord.
- distal end 25 includes selectively extendable needles 26.
- needles 26 are configured to flare outward when extended beyond distal end 25 of the apparatus, thereby enhancing dispersal of the bioactive agent into tissue mass T.
- needles 26 are configured to delivery bioactive agent B into the tissue mass while minimizing barotrauma to the bioactive agent and the injury to the tissue mass.
- distal end 25 illustratively includes three needles 26, a greater or lesser number of needles may be employed without departing from the spirit of the present invention.
- Apparatus 30 comprises handle 31 that is configured to accept conventional syringe 28, which may be loaded with a preselected bioactive agent, such as stem cells in a nutrient solution.
- the barrel of syringe 28 is removably coupled to tube 32 via fluid-tight seal 33.
- Tube 32 which carries one or more tissue-piercing needles at its distal end, is arranged to reciprocate through sleeve 34 so that the distal tip of the needle extends beyond bushing 35 when the device is actuated.
- Piston 29 of syringe 28 is removably coupled to block 36 and rails 37.
- Handle 31 includes trigger 38 that may be depressed to selectively actuate apparatus 30.
- trigger 38 is coupled to tube 33 via gear train 39 and linkage 40.
- Clamp 41 is configured to grip and reciprocate the body of the syringe in accordance with the degree of actuation of trigger 38.
- Each of rails 37 preferably includes a portion that forms a rack to permit forward movement of piston 29 of the syringe during a first range of motion of trigger 38, and then retain piston 29 stationary relative to rails 37 during a second range of motion of the trigger.
- Link 40 is coupled to clamp 41 so that, after syringe 28 and piston 29 are advanced during the initial range of motion of the trigger, the piston is held stationary while clamp 41 retracts tube 32 and needles from the needle track(s) and simultaneously urges the body of syringe 28 proximally.- This motion causes the bioactive material within syringe 28 to be dispensed into the needle track(s) (see FIGS. 2 and 3) at low velocity and with little or no barotrauma. As depicted in FIG. 4B
- trigger 38 and link 40 preferably are biased by springs 42 and 43, respectively, to return the mechanism to its starting position when trigger 38 is released.
- FIGS. 5A-5C operation of apparatus 30 is described.
- trigger 38 of the apparatus is shown at its initial position, and with syringe 28 and piston 29 in the proximal-most positions.
- gear train 39 and link 40 urge syringe 28, piston 29 and rails 37 in the distal direction in unison. This in turn causes tube 32 and clamp 41 to be advanced distally, in turn causing needles 43 to extend beyond bushing 35.
- the tissue-piercing end of tube 33 includes three needles 43 that flare outward upon entering into a tissue mass, as depicted in FIG. 3A. Because syringe 28 and piston 29 are moved in unison, the bioactive agent contained within syringe 28 is subjected to substantially no hydraulic forces, and the distance between block 36 and the proximal-most portion of syringe 28 remains unchanged. As further depicted in FIG. 5C, continued depression of trigger 38 causes link 40 to begin retracting tube 32 in the proximal direction. This motion also drives clamp 41 in the proximal direction. Because the rack portions of rails 37 disengage from link 40 and gear train 39 during proximal movement of clamp 41, rails 37 and piston 29 remain stationary.
- proximal movement of clamp 41 and tube 32 both retracts needles 43 from the needle tracks formed in the tissue, and urges the body of syringe 28 against piston 29. Still referring to FIG. 5C, proximal translation of claim 41 also causes the distance between block 36 and the proximal-most portion of syringe 28 to shorten. This action applies sufficient pressure to the contents of syringe 28 to dispense the bioactive agent into the needle tracks formed by needles 43 as the needles withdraw from the tissue.
- springs 42 and 43 return tube 32 and clamp 41 to the starting position, shown in FIG. 5A. Apparatus 30 then may be repositioned, and the above process repeated.
- the volume of injected material delivered into the target tissue may be adjusted depending upon the target tissue milieu. For example, for tissue or muscle that is fairly elastic, such as heart muscle, additional material may be injected to create low-pressure compartments within the tissue. On the other hand, lower volumes may be employed in less resilient structures, such as the spinal cord and brain.
- needles 43' of apparatus 30 may have different predetermined lengths so as to deliver the bioactive agent at various depths within spinal cord S to treat injured region D. As illustrated in FIG.
- needles 43' may first be used to deliver bioactive agent on a first side of a severed region D of spinal cord S, and then moved and applied to the opposite side of the severed region (shown in dotted line in FIG. 6A) . Additionally, needles 43' may be arranged to be individually rotated so that the bioactive agent is dispersed in preselected directions.
- FIG. 6B depicts the use of grid 50 to guide needles 43' into predetermined locations along spinal cord S.
- Grid 50 comprises block 51 having a plurality of through holes 52 disposed along its surface to provide a predetermined separation between injection regions.
- grid 50 lends structural support to damaged spinal region D during stem cell injection.
- apparatus 30 may be used to inject needles 43' at a first location, and then repositioned using grid 50 (as shown in dotted line) to provide subsequent injections.
- a predetermined amount of cerebrospinal fluid may be removed from spinal cord S prior to injecting the bioactive agent.
- the amount of cerebrospinal fluid removed is substantially equivalent to the amount of bioactive agent, e.g., stem cell solution, injected into the spinal cord.
- This step of the method is expected to enhance atraumatic delivery of the bioactive agent by reducing the risk that the injection prevents injury to the spinal artery or surrounding delicate tissue during injection.
- Needle 60 comprises a glass or polymer microfiber adapted to receive and transmit electric signals, and includes tissue-piercing distal end 61 and interior lumen 62. Needle 60 is loaded with a bioactive agent, preferably comprising stem cells 65, and in addition is coupled to power supply 63 that applies an electric field longitudinally along needle 60.
- a bioactive agent preferably comprising stem cells 65
- an electric field is applied to needle 60 that attracts negatively charged stem cells 65 toward end 61, where they are deposited into the spinal cord.
- a positive charge is induced at distal end 61 of needle 60, thereby causing negatively charged stem cells ' 65 to be drawn to the distal tip of the needle.
- Stem cells 95 are believed to be negatively charged in the natural state, so that they are drawn toward the positive charge at distal end 61 of needle 60.
- an ionic solution containing negatively charged particles may be added to the bioactive agent prior to injection to increase the attraction of the stem cells towards a positive charge.
- Needle 70 comprises an electroactive polymer that forms an actuator, and includes tissue-piercing distal end .71 and interior lumen 72. Needle 70 is loaded with a bioactive agent, preferably comprising stem cells 75, and is coupled to power supply 73 that applies an electric field longitudinally along needle 70. Electroactive polymers are members of the family of plastics referred to as "conducting polymers,” and are preferred for the practice of the present invention due to their small size, large force and strain and low cost.
- injection needle 70 comprises an electroactive polymer that is adapted to contract in response to electrical stimulation.
- Suitable electroactive polymers include, but are not limited to, polypyrrole, polyacetylene, polyaniline and polysulfone. Oxidation or reduction of these polymers leads to a charge imbalance that results in a flow of ions (dopants) into the material in order to balance charge.
- the ions enter the polymer from an ionically conductive electrolyte medium that is coupled to the polymer surface. Conversely, if ions are already present in the polymer when it is oxidized or reduced, they may exit the polymer. Dimensional changes in electroactive polymers may be triggered by the mass transfer of ions into or out of the polymer.
- the expansion is due to ion insertion between chains, whereas repulsion between chains is the dominant effect for other electroactive polymers.
- the mass transfer of ions into and out of the electroactive polymer leads to an expansion or contraction of the polymer.
- needle 70 may be contracted such that a predetermined amount of bioactive agent is ejected from distal end 71 of the needle.
- needle 70 comprises an electroactive polymer that is configured to contract when an electric charge is applied to the needle by power supply 73.
- Needle 70 has a first diameter (FIG. 8A) and a second, contracted diameter (FIG. 8B) when energized.
- FIG. 8B when an electrostatic charge is applied to injection needle 70, the needle contracts and the diameter of lumen 72 decreases, thereby expelling a predetermined amount of stem cells 75 out of distal end 71 of needle 70.
- constriction of lumen 72 is a bulk phenomenon that imposes a low-level distributed compressive force to the bioactive agent disposed in the lumen. Accordingly, substantially smaller local pressure fluctations will be imposed on the bioactive agent as compared to pressurized injection using a syringe, thereby reducing barotrauma and leading to substantially better viability of the implanted stem cells.
- the embodiments of FIGS. 7 and 8 may include multiple needle tips to deliver bioactive agent at several sites or depths simultaneously, and may be used with a grid, such as described with respect to FIGS. 6, to deliver the bioactive agent according to a predetermined pattern.
- power supplies 63 and 73 of the embodiments of FIGS. 7 and 8, respectively may include controllers that control operation of the electric fields applied to the needles so that predetermined amounts of bioactive agent are delivered by the needles when activated.
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2574089A CA2574089C (en) | 2004-07-19 | 2005-07-18 | Apparatus and methods for treating tissue using passive injection systems |
EP05772267A EP1773440B1 (en) | 2004-07-19 | 2005-07-18 | Apparatus for treating tissue using passive injection systems |
AT05772267T ATE526054T1 (en) | 2004-07-19 | 2005-07-18 | DEVICE FOR TREATING TISSUE USING PASSIVE INJECTION SYSTEMS |
JP2007522630A JP4934029B2 (en) | 2004-07-19 | 2005-07-18 | Apparatus and method for treating tissue using a passive injection system |
AU2005274774A AU2005274774B2 (en) | 2004-07-19 | 2005-07-18 | Apparatus for treating tissue using passive injection systems |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
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US10/894,810 | 2004-07-19 | ||
US10/894,810 US7632262B2 (en) | 2004-07-19 | 2004-07-19 | Systems and methods for atraumatic implantation of bio-active agents |
US10/977,594 | 2004-10-29 | ||
US10/977,594 US7338471B2 (en) | 2004-07-19 | 2004-10-29 | Apparatus and methods for treating tissue using passive injection systems |
Publications (2)
Publication Number | Publication Date |
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WO2006020255A2 true WO2006020255A2 (en) | 2006-02-23 |
WO2006020255A3 WO2006020255A3 (en) | 2006-11-30 |
Family
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Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
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PCT/US2005/025469 WO2006020255A2 (en) | 2004-07-19 | 2005-07-18 | Apparatus and methods for treating tissue using passive injection systems |
PCT/US2005/025470 WO2006020256A2 (en) | 2004-07-19 | 2005-07-18 | Systems and methods for atraumatic implantation of bio-active agents |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
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PCT/US2005/025470 WO2006020256A2 (en) | 2004-07-19 | 2005-07-18 | Systems and methods for atraumatic implantation of bio-active agents |
Country Status (7)
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US (5) | US7632262B2 (en) |
EP (4) | EP1773440B1 (en) |
JP (2) | JP4934029B2 (en) |
AT (1) | ATE526054T1 (en) |
AU (2) | AU2005274775B2 (en) |
CA (2) | CA2573844C (en) |
WO (2) | WO2006020255A2 (en) |
Families Citing this family (52)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7632262B2 (en) * | 2004-07-19 | 2009-12-15 | Nexeon Medical Systems, Inc. | Systems and methods for atraumatic implantation of bio-active agents |
US20090118673A1 (en) * | 2007-11-07 | 2009-05-07 | Jerett Creed | Needle injection catheter |
US8043257B2 (en) * | 2007-12-06 | 2011-10-25 | Abbott Cardiovascular Systems Inc. | Agent delivery catheter having an inflation bridge between two axially spaced balloons |
US8133208B2 (en) * | 2008-04-02 | 2012-03-13 | Bsecs Holdings, Llc | Injection control method and device |
US20090254060A1 (en) * | 2008-04-02 | 2009-10-08 | Hetherington Hugh E | Motor assembly for injection control device |
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