WO2006069289A3 - Polypeptides having glucoamylase activity and polynucleotides encoding same - Google Patents

Polypeptides having glucoamylase activity and polynucleotides encoding same Download PDF

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Publication number
WO2006069289A3
WO2006069289A3 PCT/US2005/046724 US2005046724W WO2006069289A3 WO 2006069289 A3 WO2006069289 A3 WO 2006069289A3 US 2005046724 W US2005046724 W US 2005046724W WO 2006069289 A3 WO2006069289 A3 WO 2006069289A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptides
polynucleotides encoding
glucoamylase activity
encoding same
relates
Prior art date
Application number
PCT/US2005/046724
Other languages
French (fr)
Other versions
WO2006069289A2 (en
Inventor
Hiroaki Udagawa
Sara Landvik
Michiko Ihara
Jiyin Liu
Chee-Leong Soong
Eric Allain
Shiro Fukuyama
Original Assignee
Novozymes North America Inc
Novozymes As
Hiroaki Udagawa
Sara Landvik
Michiko Ihara
Jiyin Liu
Chee-Leong Soong
Eric Allain
Shiro Fukuyama
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes North America Inc, Novozymes As, Hiroaki Udagawa, Sara Landvik, Michiko Ihara, Jiyin Liu, Chee-Leong Soong, Eric Allain, Shiro Fukuyama filed Critical Novozymes North America Inc
Priority to ES05855307.4T priority Critical patent/ES2552635T3/en
Priority to DK05855307.4T priority patent/DK1831384T3/en
Priority to EP05855307.4A priority patent/EP1831384B1/en
Priority to AU2005319073A priority patent/AU2005319073B2/en
Priority to CA2591745A priority patent/CA2591745C/en
Priority to MX2007007107A priority patent/MX2007007107A/en
Priority to JP2007548501A priority patent/JP5160899B2/en
Priority to CN2005800443174A priority patent/CN101194015B/en
Publication of WO2006069289A2 publication Critical patent/WO2006069289A2/en
Publication of WO2006069289A3 publication Critical patent/WO2006069289A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

The present invention relates to polypeptides having glucoamylase activity and isolated polynucleotides encoding said polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. The invention also relates to the composition comprising a glucoamylase of the invention as well as the use such compositions for starch conversion processes, brewing, including processes for producing fermentation products or syrups.
PCT/US2005/046724 2004-12-22 2005-12-22 Polypeptides having glucoamylase activity and polynucleotides encoding same WO2006069289A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
ES05855307.4T ES2552635T3 (en) 2004-12-22 2005-12-22 Polypeptides with glucoamylase activity and polynucleotide coding
DK05855307.4T DK1831384T3 (en) 2004-12-22 2005-12-22 Polypeptides with glucoamylase activity and polynucleotides encoding them.
EP05855307.4A EP1831384B1 (en) 2004-12-22 2005-12-22 Polypeptides having glucoamylase activity and polynucleotides encoding same
AU2005319073A AU2005319073B2 (en) 2004-12-22 2005-12-22 Polypeptides having glucoamylase activity and polynucleotides encoding same
CA2591745A CA2591745C (en) 2004-12-22 2005-12-22 Polypeptides having glucoamylase activity and polynucleotides encoding same
MX2007007107A MX2007007107A (en) 2004-12-22 2005-12-22 Polypeptides having glucoamylase activity and polynucleotides encoding same.
JP2007548501A JP5160899B2 (en) 2004-12-22 2005-12-22 Polypeptides having glucoamylase activity and polynucleotides encoding them
CN2005800443174A CN101194015B (en) 2004-12-22 2005-12-22 Polypeptides having glucoamylase activity and polynucleotides encoding same

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US63861404P 2004-12-22 2004-12-22
US60/638,614 2004-12-22
US65061205P 2005-02-07 2005-02-07
US60/650,612 2005-02-07

Publications (2)

Publication Number Publication Date
WO2006069289A2 WO2006069289A2 (en) 2006-06-29
WO2006069289A3 true WO2006069289A3 (en) 2007-05-31

Family

ID=36602341

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2005/046725 WO2006069290A2 (en) 2004-12-22 2005-12-22 Enzymes for starch processing
PCT/US2005/046724 WO2006069289A2 (en) 2004-12-22 2005-12-22 Polypeptides having glucoamylase activity and polynucleotides encoding same

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2005/046725 WO2006069290A2 (en) 2004-12-22 2005-12-22 Enzymes for starch processing

Country Status (10)

Country Link
US (5) US7326548B2 (en)
EP (3) EP1831385B1 (en)
JP (2) JP5160899B2 (en)
AR (1) AR051864A1 (en)
AU (2) AU2005319074B2 (en)
CA (2) CA2591745C (en)
DK (3) DK1831385T3 (en)
ES (3) ES2552635T3 (en)
MX (2) MX2007007030A (en)
WO (2) WO2006069290A2 (en)

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