WO2006085972A3 - Compositions and processes for the extraction and detection of microbial atp - Google Patents

Compositions and processes for the extraction and detection of microbial atp Download PDF

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Publication number
WO2006085972A3
WO2006085972A3 PCT/US2005/023545 US2005023545W WO2006085972A3 WO 2006085972 A3 WO2006085972 A3 WO 2006085972A3 US 2005023545 W US2005023545 W US 2005023545W WO 2006085972 A3 WO2006085972 A3 WO 2006085972A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
compositions
extraction
processes
atp
Prior art date
Application number
PCT/US2005/023545
Other languages
French (fr)
Other versions
WO2006085972A9 (en
WO2006085972A2 (en
Inventor
Frank Fan
Braeden Butler
Keith V Wood
Original Assignee
Promega Corp
Frank Fan
Braeden Butler
Keith V Wood
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp, Frank Fan, Braeden Butler, Keith V Wood filed Critical Promega Corp
Priority to JP2007520407A priority Critical patent/JP5255273B2/en
Priority to AT05857498T priority patent/ATE429507T1/en
Priority to EP05857498A priority patent/EP1763584B1/en
Priority to DK05857498T priority patent/DK1763584T3/en
Priority to DE602005014110T priority patent/DE602005014110D1/en
Priority to CA002572608A priority patent/CA2572608A1/en
Publication of WO2006085972A2 publication Critical patent/WO2006085972A2/en
Publication of WO2006085972A3 publication Critical patent/WO2006085972A3/en
Publication of WO2006085972A9 publication Critical patent/WO2006085972A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia

Abstract

The present invention is directed to compositions and methods for single step extraction and detection of ATP levels from microbial cells. The disclosed compositions are formulated to efficiently elicit bioluminescent detection of ATP among a broad variety of different microorganisms using a common single-step reagent composition. Additional luminescence-based methods are provided for identifying other useful extracting agents or for screening compounds for their pharmaceutical or biological effects on microbial cells.
PCT/US2005/023545 2004-07-02 2005-07-01 Compositions and processes for the extraction and detection of microbial atp WO2006085972A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2007520407A JP5255273B2 (en) 2004-07-02 2005-07-01 Microbial ATP extraction and detection system
AT05857498T ATE429507T1 (en) 2004-07-02 2005-07-01 COMPOSITIONS AND METHODS FOR EXTRACTION AND DETECTION OF MICROBIAL ATP
EP05857498A EP1763584B1 (en) 2004-07-02 2005-07-01 Compositions and processes for the extraction and detection of microbial atp
DK05857498T DK1763584T3 (en) 2004-07-02 2005-07-01 Compositions and Methods for the Extraction and Detection of Microbial ATP
DE602005014110T DE602005014110D1 (en) 2004-07-02 2005-07-01 COMPOSITIONS AND METHODS OF EXTRACTION AND DETECTION OF MICROBIAL ATP
CA002572608A CA2572608A1 (en) 2004-07-02 2005-07-01 Microbial atp extraction and detection system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58497104P 2004-07-02 2004-07-02
US60/584,971 2004-07-02

Publications (3)

Publication Number Publication Date
WO2006085972A2 WO2006085972A2 (en) 2006-08-17
WO2006085972A3 true WO2006085972A3 (en) 2007-01-04
WO2006085972A9 WO2006085972A9 (en) 2007-02-15

Family

ID=36793507

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/023545 WO2006085972A2 (en) 2004-07-02 2005-07-01 Compositions and processes for the extraction and detection of microbial atp

Country Status (10)

Country Link
US (2) US7422868B2 (en)
EP (1) EP1763584B1 (en)
JP (1) JP5255273B2 (en)
CN (1) CN101040054A (en)
AT (1) ATE429507T1 (en)
CA (1) CA2572608A1 (en)
DE (1) DE602005014110D1 (en)
DK (1) DK1763584T3 (en)
ES (1) ES2324952T3 (en)
WO (1) WO2006085972A2 (en)

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Also Published As

Publication number Publication date
US7422868B2 (en) 2008-09-09
WO2006085972A9 (en) 2007-02-15
DK1763584T3 (en) 2009-08-03
ES2324952T3 (en) 2009-08-20
JP2008504841A (en) 2008-02-21
JP5255273B2 (en) 2013-08-07
US20090029398A1 (en) 2009-01-29
CA2572608A1 (en) 2006-08-17
DE602005014110D1 (en) 2009-06-04
EP1763584A2 (en) 2007-03-21
CN101040054A (en) 2007-09-19
ATE429507T1 (en) 2009-05-15
EP1763584B1 (en) 2009-04-22
WO2006085972A2 (en) 2006-08-17
US20060008860A1 (en) 2006-01-12

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