WO2006091868A3 - High throughput screening of molecular libraries using laser induced sorting - Google Patents

High throughput screening of molecular libraries using laser induced sorting Download PDF

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Publication number
WO2006091868A3
WO2006091868A3 PCT/US2006/006696 US2006006696W WO2006091868A3 WO 2006091868 A3 WO2006091868 A3 WO 2006091868A3 US 2006006696 W US2006006696 W US 2006006696W WO 2006091868 A3 WO2006091868 A3 WO 2006091868A3
Authority
WO
WIPO (PCT)
Prior art keywords
high throughput
throughput screening
libraries
laser induced
molecular libraries
Prior art date
Application number
PCT/US2006/006696
Other languages
French (fr)
Other versions
WO2006091868A2 (en
Inventor
Julie Sutcliffe-Goulden
Sven Hausner
Christian Leutenegger
Marco Estrada
Original Assignee
Univ California
Julie Sutcliffe-Goulden
Sven Hausner
Christian Leutenegger
Marco Estrada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Julie Sutcliffe-Goulden, Sven Hausner, Christian Leutenegger, Marco Estrada filed Critical Univ California
Publication of WO2006091868A2 publication Critical patent/WO2006091868A2/en
Publication of WO2006091868A3 publication Critical patent/WO2006091868A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Abstract

High throughput screening of libraries comprising particulate support is provided using laser catapulting in which a laser beam delivers a pulse to the support and catapults it into a vessel for collection. The methods are useful with any assay format, including cell-based and cell-free formats and requires only that binding of a target to a library element can be identified, preferably using optical techniques. The libraries and targets each may comprise peptides, proteins, nucleic acids, peptide nucleic acids, carbohydrates, lipids and small organic molecule compounds.
PCT/US2006/006696 2005-02-24 2006-02-24 High throughput screening of molecular libraries using laser induced sorting WO2006091868A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US65676105P 2005-02-24 2005-02-24
US60/656,761 2005-02-24

Publications (2)

Publication Number Publication Date
WO2006091868A2 WO2006091868A2 (en) 2006-08-31
WO2006091868A3 true WO2006091868A3 (en) 2006-11-09

Family

ID=36928069

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/006696 WO2006091868A2 (en) 2005-02-24 2006-02-24 High throughput screening of molecular libraries using laser induced sorting

Country Status (1)

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WO (1) WO2006091868A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011025976A2 (en) * 2009-08-28 2011-03-03 On-Q-Ity Inc. Methods of detaching and collecting cells
US9328366B2 (en) * 2011-10-27 2016-05-03 Snu R & Db Foundation Method for mass production of high-purity oligonucleotides

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5460964A (en) * 1992-04-03 1995-10-24 Regents Of The University Of Minnesota Method for culturing hematopoietic cells
US5639603A (en) * 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
US5998129A (en) * 1996-02-05 1999-12-07 P.A.L.M. Gmbh Method and device for the contactless laser-assisted microinjection, sorting and production of biological objects generated in a planar manner
US6306613B1 (en) * 1997-02-26 2001-10-23 Ciblex Corporation Modulators of leaderless protein export and methods for identifying and using the same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639603A (en) * 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
US5460964A (en) * 1992-04-03 1995-10-24 Regents Of The University Of Minnesota Method for culturing hematopoietic cells
US5998129A (en) * 1996-02-05 1999-12-07 P.A.L.M. Gmbh Method and device for the contactless laser-assisted microinjection, sorting and production of biological objects generated in a planar manner
US6306613B1 (en) * 1997-02-26 2001-10-23 Ciblex Corporation Modulators of leaderless protein export and methods for identifying and using the same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KAMARCK M.E.: "Fluorescence-Activated Cell Sorting of Hybrid and Transfected Cells", METH. ENZYMOL., vol. 151, 1987, pages 150 - 165, XP008065797 *
LAM ET AL.: "A new type of synthetic peptide library for identifying ligand-binding activity", NATURE, vol. 354, 7 November 1991 (1991-11-07), pages 82 - 84, XP002913659 *

Also Published As

Publication number Publication date
WO2006091868A2 (en) 2006-08-31

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