WO2006122083A3 - Water relaxation-based sensors - Google Patents

Water relaxation-based sensors Download PDF

Info

Publication number
WO2006122083A3
WO2006122083A3 PCT/US2006/017842 US2006017842W WO2006122083A3 WO 2006122083 A3 WO2006122083 A3 WO 2006122083A3 US 2006017842 W US2006017842 W US 2006017842W WO 2006122083 A3 WO2006122083 A3 WO 2006122083A3
Authority
WO
WIPO (PCT)
Prior art keywords
analyte
nanoparticles
sensor
magnetic
chamber
Prior art date
Application number
PCT/US2006/017842
Other languages
French (fr)
Other versions
WO2006122083A2 (en
Inventor
Lee Josephson
Yi Sun
Ralph Weissleder
Original Assignee
Gen Hospital Corp
Lee Josephson
Yi Sun
Ralph Weissleder
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Hospital Corp, Lee Josephson, Yi Sun, Ralph Weissleder filed Critical Gen Hospital Corp
Priority to JP2008511257A priority Critical patent/JP5021629B2/en
Priority to CN2006800250585A priority patent/CN101253416B/en
Priority to CA2606924A priority patent/CA2606924C/en
Priority to EP06752434A priority patent/EP1880228B1/en
Priority to AT06752434T priority patent/ATE515711T1/en
Publication of WO2006122083A2 publication Critical patent/WO2006122083A2/en
Publication of WO2006122083A3 publication Critical patent/WO2006122083A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/72—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables
    • G01N27/74—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids
    • G01N27/745—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids for detecting magnetic beads used in biochemical assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/145—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue
    • A61B5/14503—Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissue invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R33/00—Arrangements or instruments for measuring magnetic variables
    • G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
    • G01R33/44—Arrangements or instruments for measuring magnetic variables involving magnetic resonance using nuclear magnetic resonance [NMR]
    • G01R33/46—NMR spectroscopy
    • G01R33/465—NMR spectroscopy applied to biological material, e.g. in vitro testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R33/00—Arrangements or instruments for measuring magnetic variables
    • G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
    • G01R33/44—Arrangements or instruments for measuring magnetic variables involving magnetic resonance using nuclear magnetic resonance [NMR]
    • G01R33/48—NMR imaging systems
    • G01R33/50—NMR imaging systems based on the determination of relaxation times, e.g. T1 measurement by IR sequences; T2 measurement by multiple-echo sequences

Abstract

This invention relates to magnetic resonance-based sensors and related methods . More specifically, the invention relates to a sensor based on magnetic resonance for detecting the presence of an analyte in a sample solution. The sensor comprises magnetic nanoparticles linked to a moiety upon which the analyte can reversibly bind, the nanoparticles being confined in a chamber with semipermeable walls which are impermeable to the nanoparticles but permeable to the analyte. Upon binding of the analyte to the nanoparticles, the latter aggregate which can be deteceted by a change in the T2 relaxation time of the sample solution within the chamber (magnetic relaxation switch) . The analyte may be a carbohydrate such as glucose, an antibody, an amino acid, a nucleic acid, an oligonucleotide, a therapeutic agent, a peptide, a protein, etc. The sensor may be used as an assay and may be read out using MRS or MRI, e.g. of well plates for high throughput anaylsis .
PCT/US2006/017842 2005-05-09 2006-05-09 Water relaxation-based sensors WO2006122083A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2008511257A JP5021629B2 (en) 2005-05-09 2006-05-09 Sensors based on water relaxation
CN2006800250585A CN101253416B (en) 2005-05-09 2006-05-09 Water relaxation-based sensors
CA2606924A CA2606924C (en) 2005-05-09 2006-05-09 Water relaxation-based sensors
EP06752434A EP1880228B1 (en) 2005-05-09 2006-05-09 Water relaxation-based sensors
AT06752434T ATE515711T1 (en) 2005-05-09 2006-05-09 WATER RELAXATION BASED SENSORS

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US67943705P 2005-05-09 2005-05-09
US60/679,437 2005-05-09

Publications (2)

Publication Number Publication Date
WO2006122083A2 WO2006122083A2 (en) 2006-11-16
WO2006122083A3 true WO2006122083A3 (en) 2007-05-03

Family

ID=37397220

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/017842 WO2006122083A2 (en) 2005-05-09 2006-05-09 Water relaxation-based sensors

Country Status (7)

Country Link
US (3) US20060269965A1 (en)
EP (2) EP1880228B1 (en)
JP (1) JP5021629B2 (en)
CN (1) CN101253416B (en)
AT (1) ATE515711T1 (en)
CA (1) CA2606924C (en)
WO (1) WO2006122083A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8883423B2 (en) 2010-10-22 2014-11-11 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7781228B2 (en) * 2005-04-07 2010-08-24 Menon & Associates, Inc. Magnetic resonance system and method to detect and confirm analytes
JP5295769B2 (en) 2005-08-31 2013-09-18 ティツー・バイオシステムズ・インコーポレーテッド NMR apparatus for detecting analytes
GB0618514D0 (en) * 2006-09-20 2006-11-01 Univ Nottingham Trent Method of detecting interactions on a microarray using nuclear magnetic resonance
CA2669045A1 (en) * 2006-11-08 2008-05-15 T2 Biosystems, Inc. Nmr systems for in vivo detection of analytes
US20110070657A1 (en) * 2007-08-17 2011-03-24 The General Hospital Corporation Detecting ions and measuring ion concentrations
WO2009026164A1 (en) * 2007-08-17 2009-02-26 The General Hospital Corporation Magnetic resonance-based viscometers and methods
DE102007039899B3 (en) * 2007-08-23 2009-04-09 Siemens Ag Sensor for enabling the detection of a substance in the body of a living being
CA2700970A1 (en) 2007-09-28 2009-04-09 T2 Biosystems, Inc. Nmr diagnostics by means of a plastic sample container
AU2008325088A1 (en) 2007-11-06 2009-05-14 T2 Biosystems, Inc. Small magnet and RF coil for magnetic resonance relaxometry
US20100322864A1 (en) * 2008-01-10 2010-12-23 President And Fellows Of Harvard College Telemetry through remote detection of nmr-active particles
US20090220434A1 (en) * 2008-02-29 2009-09-03 Florida State University Research Foundation Nanoparticles that facilitate imaging of biological tissue and methods of forming the same
US9161714B2 (en) * 2008-08-21 2015-10-20 Palo Alto Research Center Incorporated Specificity of analyte detection in etalons
AU2009308841B2 (en) 2008-10-29 2014-07-17 T2 Biosystems, Inc. NMR detection of coagulation time
EP2309266A1 (en) * 2009-09-21 2011-04-13 F. Hoffmann-La Roche AG Method for carrying out reactions in an analytical device
US8741591B2 (en) 2009-10-09 2014-06-03 The Research Foundation For The State University Of New York pH-insensitive glucose indicator protein
CN101776738A (en) * 2009-12-30 2010-07-14 复旦大学 Magnetic relaxation switch based on Fe304@Au and detection method thereof
CN102200571A (en) * 2010-03-26 2011-09-28 复旦大学 Magnetic relaxation switch-based detection method
JP2013526616A (en) 2010-05-26 2013-06-24 ザ ジェネラル ホスピタル コーポレイション Magnetic nanoparticles
CN101858964A (en) * 2010-06-03 2010-10-13 复旦大学 Magnetic relaxation switch for detecting glycoprotein
EP3974831A1 (en) 2010-10-22 2022-03-30 T2 Biosystems, Inc. Methods for detecting the presence of a bacterium in a whole blood sample
US8563298B2 (en) * 2010-10-22 2013-10-22 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
CN103917658B (en) 2011-07-13 2016-09-21 T2生物系统公司 For monitoring the NMR method that clot is formed
WO2013043858A1 (en) 2011-09-21 2013-03-28 T2 Biosystems, Inc. Nmr methods for endotoxin analysis
US9958416B2 (en) 2011-11-23 2018-05-01 The General Hospital Corporation Analyte detection using magnetic hall effect
US9562271B2 (en) 2012-04-20 2017-02-07 T2 Biosystems, Inc. Compositions and methods for detection of Candida species
JP6382188B2 (en) 2012-06-25 2018-08-29 ザ ジェネラル ホスピタル コーポレイション Particle sorting method using high gradient magnetic field
JP2014095691A (en) * 2012-09-13 2014-05-22 Aspect Imaging Ltd Means and method for using paramagnetic materials for in vitro diagnostic applications
US9739733B2 (en) 2012-12-07 2017-08-22 T2 Biosystems, Inc. Methods for monitoring tight clot formation
CN104871026B (en) 2012-12-17 2020-01-10 通用电气公司 In vitro magnetic resonance detection of target substances
US9678185B2 (en) 2013-03-15 2017-06-13 Pepsico, Inc. Method and apparatus for measuring physico-chemical properties using a nuclear magnetic resonance spectrometer
CN103217530B (en) * 2013-03-26 2015-01-28 南昌大学 NMR food-borne pathogen rapid detection method based on paramagnetic nano-Fe-Co alloy probe indirect enrichment
EP2984482B1 (en) 2013-04-12 2020-09-09 University of Maryland, Baltimore Biopharmaceutical aggregation assessment using magnetic resonance relaxometry
CN103468810B (en) * 2013-09-17 2014-10-22 江南大学 Method for detecting DNA (Deoxyribose Nucleic Acid) based on chiral nano material
EP3058316B1 (en) 2013-10-18 2019-03-13 The General Hospital Corporation Microfluidic sorting using high gradient magnetic fields
EP3745139A1 (en) 2014-11-03 2020-12-02 The General Hospital Corporation Concentrating particles in a microfluidic device
CN105197635B (en) * 2015-10-20 2017-03-22 京东方科技集团股份有限公司 Display panel processing platform
JP2019512208A (en) 2016-01-21 2019-05-16 ティー２ バイオシステムズ，インコーポレーテッド NMR method and system for detecting bacteria quickly
EP3547914A4 (en) 2016-12-02 2020-11-11 University Of Maryland, Baltimore Solvent nuclear magnetic resonance for noninvasive inspection of particle-containing products
CN107478834A (en) * 2017-09-05 2017-12-15 杨蕾 A kind of preparation method for detecting salmonella magnetic resonance imaging kit
CN108120794A (en) * 2017-12-21 2018-06-05 深圳市赛亿科技开发有限公司 A kind of method of concentration of glucose in Intelligent cup and its detection beverage
US11543371B2 (en) 2018-10-04 2023-01-03 University Of Maryland, Baltimore In situ, real-time in-line detection of filling errors in pharmaceutical product manufacturing using water proton NMR
US11585770B2 (en) 2018-10-04 2023-02-21 University Of Maryland, Baltimore In situ determination of alum filling evenness and sedimentation in pharmaceutical products using water proton NMR
EP4076537A4 (en) 2019-12-20 2024-02-28 Univ Maryland Noninvasive quantitation of full versus empty capsids using water proton nmr
BR112022013206A2 (en) 2019-12-31 2022-09-13 Univ Santiago Chile SYSTEM AND METHOD FOR DETECTION OF BIOLOGICAL ANALYTE, WHICH INCLUDES A MICRO-ORGANISM, BY CHANGE IN MAGNETIC PROPERTY OF A SUBSTRATE, WHICH USES SUPERPARAMAGNETIC NANOPARTICLES
US11914013B2 (en) 2020-08-18 2024-02-27 University Of Maryland, Baltimore Real-time in situ monitoring of suspension sedimentation using water proton NMR

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991017428A1 (en) * 1990-05-03 1991-11-14 Advanced Magnetics Inc. Solvant mediated relaxation assay system
US6297062B1 (en) * 1996-03-07 2001-10-02 Bio-Magnetics Ltd. Separation by magnetic particles
WO2002098364A2 (en) * 2001-06-06 2002-12-12 The General Hospital Corporation Magnetic-nanoparticle conjugates and methods of use
US20030174384A1 (en) * 2001-10-24 2003-09-18 Wm. Marsh Rice University Nanoparticle-based all-optical sensors
WO2005061724A1 (en) * 2003-12-10 2005-07-07 The General Hospital Corporation Self-assembling nanoparticle conjugates

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5262176A (en) 1986-07-03 1993-11-16 Advanced Magnetics, Inc. Synthesis of polysaccharide covered superparamagnetic oxide colloids
US4875486A (en) * 1986-09-04 1989-10-24 Advanced Techtronics, Inc. Instrument and method for non-invasive in vivo testing for body fluid constituents
US5136095A (en) * 1987-05-19 1992-08-04 Syntex (U.S.A.) Inc. Reversible agglutination mediators
US5153166A (en) * 1988-08-18 1992-10-06 Trustees Of At Biochem Chromatographic stationary supports
JP3020271B2 (en) 1988-11-21 2000-03-15 ダイナル・エイ・エス Nucleic acid probe
US5254460A (en) * 1990-05-03 1993-10-19 Advanced Magnetics, Inc. Solvent mediated relaxation assay system
IL98744A0 (en) 1990-07-06 1992-07-15 Gen Hospital Corp Method of studying biological tissue using monocrystalline particles
US5445971A (en) * 1992-03-20 1995-08-29 Abbott Laboratories Magnetically assisted binding assays using magnetically labeled binding members
US6256522B1 (en) * 1992-11-23 2001-07-03 University Of Pittsburgh Of The Commonwealth System Of Higher Education Sensors for continuous monitoring of biochemicals and related method
US6097188A (en) * 1995-01-31 2000-08-01 The Board Of Trustees Of The University Of Illinois Microcoil based micro-NMR spectrometer and method
US6500343B2 (en) * 1995-02-21 2002-12-31 Iqbal W. Siddiqi Method for mixing and separation employing magnetic particles
CA2253710A1 (en) 1996-04-25 1997-10-30 Spectrametrix Inc. Analyte assay using particulate labels
WO1997046882A1 (en) * 1996-06-07 1997-12-11 Immunivest Corporation Magnetic separation employing external and internal gradients
DE69737598T2 (en) 1996-07-29 2007-12-27 Nanosphere Inc., Skokie NANOPARTICLES WITH OLIGONUCLEOTIDES ADDITED TO IT AND THEIR USES
US6361944B1 (en) * 1996-07-29 2002-03-26 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
FR2758884B1 (en) * 1997-01-30 1999-04-02 Bio Merieux METHOD FOR ISOLATING, IN PARTICULAR DETECTING OR QUANTIFYING AN ANALYTE IN A MEDIUM
US6194900B1 (en) * 1998-06-19 2001-02-27 Agilent Technologies, Inc. Integrated miniaturized device for processing and NMR detection of liquid phase samples
US6326083B1 (en) * 1999-03-08 2001-12-04 Calipher Technologies Corp. Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
US6599498B1 (en) 1999-04-09 2003-07-29 Advanced Magnetics, Inc. Heat stable colloidal iron oxides coated with reduced carbohydrates and carbohdrate derivatives
CA2376623C (en) 1999-06-25 2011-04-19 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
JP2003528289A (en) 1999-08-06 2003-09-24 インスティテュート・フュア・ディアグノスティクフォルシュンク・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング・アン・デア・フライエン・ウニヴエアズィテート・ベルリン A method for detecting binding reactions using measurements of the relaxation of the birefringence of magnetic particles
BR0014252A (en) 1999-09-14 2002-11-19 Biomedical Apherese Systeme Gm Magnetic nanoparticles having biochemical activity, method for their production and uses
US6254830B1 (en) * 1999-11-05 2001-07-03 The Board Of Governors For Higher Education, State Of Rhode Island And Providence Plantations Magnetic focusing immunosensor for the detection of pathogens
US6751491B2 (en) * 2001-09-01 2004-06-15 M Biotech Inc Analyte measuring biosensor chip using image scanning system
US7851593B2 (en) * 2002-01-04 2010-12-14 Becton, Dickinson And Company Binding proteins as biosensors
US7018849B2 (en) * 2002-01-15 2006-03-28 Piasio Roger N Process for (A) separating biological/ligands from dilute solutions and (B) conducting an immunochromatographic assay thereof employing superparamagnetic particles throughtout
US6798200B2 (en) * 2002-06-03 2004-09-28 Long-Sheng Fan Batch-fabricated gradient and RF coils for submicrometer resolution magnetic resonance imaging and manipulation
EP1735668A2 (en) 2004-04-13 2006-12-27 President And Fellows Of Harvard College Methods and apparatus for manipulation and/or detection of biological samples and other objects

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991017428A1 (en) * 1990-05-03 1991-11-14 Advanced Magnetics Inc. Solvant mediated relaxation assay system
US6297062B1 (en) * 1996-03-07 2001-10-02 Bio-Magnetics Ltd. Separation by magnetic particles
WO2002098364A2 (en) * 2001-06-06 2002-12-12 The General Hospital Corporation Magnetic-nanoparticle conjugates and methods of use
US20030174384A1 (en) * 2001-10-24 2003-09-18 Wm. Marsh Rice University Nanoparticle-based all-optical sensors
WO2005061724A1 (en) * 2003-12-10 2005-07-07 The General Hospital Corporation Self-assembling nanoparticle conjugates

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GIJS MAM: "Magnetic bead handling on-chip: new opportunities for analytical applications", MICROFLUID NANOFLUID, vol. 1, 2004, pages 22 - 40, XP002418117 *
MANUEL PEREZ J ET AL: "Magnetic relaxation switches capable of sensing molecular interactions", NATURE BIOTECHNOLOGY, vol. 20, August 2002 (2002-08-01), pages 816 - 820, XP002418116 *
MANUEL PEREZ J ET AL: "Use of magnetic nanoparticles as nanosensors to probe for molecular interactions", CHEMBIOCHEM, WILEY-VCH VERLAG, vol. 5, 26 February 2004 (2004-02-26), Weinheim, Germany, pages 261 - 264, XP002418115 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8883423B2 (en) 2010-10-22 2014-11-11 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes

Also Published As

Publication number Publication date
WO2006122083A2 (en) 2006-11-16
US20060269965A1 (en) 2006-11-30
ATE515711T1 (en) 2011-07-15
CA2606924C (en) 2013-04-16
CN101253416A (en) 2008-08-27
EP1880228B1 (en) 2011-07-06
US20140017816A1 (en) 2014-01-16
EP2385386A1 (en) 2011-11-09
US8535949B2 (en) 2013-09-17
CA2606924A1 (en) 2006-11-16
EP1880228A2 (en) 2008-01-23
JP5021629B2 (en) 2012-09-12
JP2008541089A (en) 2008-11-20
US20100120174A1 (en) 2010-05-13
EP2385386B1 (en) 2012-11-14
CN101253416B (en) 2011-08-24

Similar Documents

Publication Publication Date Title
WO2006122083A3 (en) Water relaxation-based sensors
Bayramoglu et al. Design of an aptamer-based magnetic adsorbent and biosensor systems for selective and sensitive separation and detection of thrombin
Kim et al. Harnessing aptamers for electrochemical detection of endotoxin
Gopinath Biosensing applications of surface plasmon resonance-based Biacore technology
Nie et al. Efficient selection of glycoprotein-binding DNA aptamers via boronate affinity monolithic capillary
Srinivas et al. Aptamer-functionalized microgel particles for protein detection
Poon et al. FRET-based modified graphene quantum dots for direct trypsin quantification in urine
Ye et al. Orientation difference of chemically immobilized and physically adsorbed biological molecules on polymers detected at the solid/liquid interfaces in situ
Ashley et al. An aptamer based surface plasmon resonance biosensor for the detection of bovine catalase in milk
WO2003025220A3 (en) Fusion proteins useful for detecting analytes
Lin et al. Selection of aptamers specific for glycated hemoglobin and total hemoglobin using on-chip SELEX
CN101313218A (en) Magnetic biosensor for determination of enzymic activity
WO2007025129A3 (en) Compositions and liquid crystals
WO2008015645A3 (en) A method of determining the concentration of an analyte using analyte sensor molecules coupled to a porous membrane
BRPI0610092B8 (en) methods for detecting an analyte and nucleic acid in a sample, and synthesizing a nucleic acid or nucleic acid analogue molecule, reagent kit, use thereof, nucleic acid or nucleic acid analogue molecule, and associated product
WO2006134546A3 (en) Accurate magnetic biosensor
EP1930445A4 (en) Kit and method for detection of urothelial cancer
Chen et al. Target-protecting dumbbell molecular probe against exonucleases digestion for sensitive detection of ATP and streptavidin
WO2006134569A3 (en) Rapid magnetic biosensor with integrated arrival time measurement
JP2008507704A5 (en)
Bai et al. Reversible detection of vancomycin using peptide-functionalized cantilever array sensor
Zhao et al. An affinity capture involved enzymatic assay for thrombin by using peptide aptamers as affinity ligands on magnetic beads
EP3864412A1 (en) Antibody or aptamer conjugated-polynucleotides and detection methods and microfluidics devices using the same
WO2007067983A3 (en) Sulfotyrosine specific antibodies and uses therefor
WO2009006877A3 (en) Determination of the activity of proteases

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 200680025058.5

Country of ref document: CN

DPE1 Request for preliminary examination filed after expiration of 19th month from priority date (pct application filed from 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2006752434

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2606924

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2008511257

Country of ref document: JP

Kind code of ref document: A

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 9272/DELNP/2007

Country of ref document: IN

NENP Non-entry into the national phase

Ref country code: RU

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 06752434

Country of ref document: EP

Kind code of ref document: A2