WO2007002210A3 - Embryonic stem cell culture compositions and methods of use thereof - Google Patents

Embryonic stem cell culture compositions and methods of use thereof Download PDF

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Publication number
WO2007002210A3
WO2007002210A3 PCT/US2006/024168 US2006024168W WO2007002210A3 WO 2007002210 A3 WO2007002210 A3 WO 2007002210A3 US 2006024168 W US2006024168 W US 2006024168W WO 2007002210 A3 WO2007002210 A3 WO 2007002210A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
cell culture
stem cell
embryonic stem
culture compositions
Prior art date
Application number
PCT/US2006/024168
Other languages
French (fr)
Other versions
WO2007002210A2 (en
Inventor
Allan J Robins
Thomas C Schulz
Stephen Dalton
E Edward Baetge
Melissa Carpenter
Original Assignee
Bresagen Inc
Univ Georgia Res Found
Cythera Inc
Robarts Res Inst
Allan J Robins
Thomas C Schulz
Stephen Dalton
E Edward Baetge
Melissa Carpenter
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bresagen Inc, Univ Georgia Res Found, Cythera Inc, Robarts Res Inst, Allan J Robins, Thomas C Schulz, Stephen Dalton, E Edward Baetge, Melissa Carpenter filed Critical Bresagen Inc
Publication of WO2007002210A2 publication Critical patent/WO2007002210A2/en
Publication of WO2007002210A3 publication Critical patent/WO2007002210A3/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/105Insulin-like growth factors [IGF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/15Transforming growth factor beta (TGF-β)

Abstract

The present invention provides compositions and methods for the culture and maintenance of undifferentiated, pluripotent mammalian stem cells. More particularly, the present invention provides a defined media useful in the absence of a feeder layer and in the absence of serum or serum replacement, that comprises an activator of IGF-IR, an activator of an FGF receptor, serum albumin, transferrin, and optionally, a member of the TGF-β family.
PCT/US2006/024168 2005-06-20 2006-06-20 Embryonic stem cell culture compositions and methods of use thereof WO2007002210A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69218605P 2005-06-20 2005-06-20
US60/692,186 2005-06-20

Publications (2)

Publication Number Publication Date
WO2007002210A2 WO2007002210A2 (en) 2007-01-04
WO2007002210A3 true WO2007002210A3 (en) 2007-07-05

Family

ID=37595805

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/024168 WO2007002210A2 (en) 2005-06-20 2006-06-20 Embryonic stem cell culture compositions and methods of use thereof

Country Status (1)

Country Link
WO (1) WO2007002210A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9018010B2 (en) 2009-11-12 2015-04-28 Technion Research & Development Foundation Limited Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
US9132226B2 (en) 2008-11-14 2015-09-15 Viacyte, Inc. Encapsulation of pancreatic cells derived from human pluripotent stem cells
US9404079B2 (en) 2005-08-29 2016-08-02 Technion Research & Development Foundation Limited Media for culturing stem cells

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
US7541185B2 (en) 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
DK2722387T3 (en) 2003-12-23 2020-01-20 Viacyte Inc Definitely endoderm
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
US7985585B2 (en) 2004-07-09 2011-07-26 Viacyte, Inc. Preprimitive streak and mesendoderm cells
WO2007059007A2 (en) 2005-11-14 2007-05-24 Cythera, Inc. Markers of definitive endoderm
AU2005272681B2 (en) 2004-08-13 2009-11-19 University Of Georgia Research Foundation, Inc. Compositions and methods for self-renewal and differentiation in human embryonic stem cells
FI20055398A0 (en) 2005-07-08 2005-07-08 Suomen Punainen Risti Veripalv Method for evaluating cell populations
ES2687233T3 (en) 2005-10-27 2018-10-24 Viacyte, Inc. Endoderm of the dorsal and ventral proximal intestine expressing PDX-1
US8153429B2 (en) 2006-02-23 2012-04-10 Viacyte, Inc. Compositions and methods useful for culturing differentiable cells
US11254916B2 (en) 2006-03-02 2022-02-22 Viacyte, Inc. Methods of making and using PDX1-positive pancreatic endoderm cells
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
SG170068A1 (en) 2006-03-02 2011-04-29 Cythera Inc Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
US9040297B2 (en) 2006-08-02 2015-05-26 Technion Research & Development Foundation Limited Methods of expanding embryonic stem cells in a suspension culture
EP2188369A4 (en) * 2007-09-04 2011-12-07 Univ Queensland A feeder cell-free culture medium and system
US7695963B2 (en) 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
US20100278788A1 (en) * 2008-01-11 2010-11-04 Bone Therapeutics, S.A. Osteogenic Differentiation Of Bone Marrow Stem Cells And Mesenchymal Stem Cells Using A Combination Of Growth Factors
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
US8895300B2 (en) 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
US9109245B2 (en) 2009-04-22 2015-08-18 Viacyte, Inc. Cell compositions derived from dedifferentiated reprogrammed cells
JP6148429B2 (en) * 2011-01-31 2017-06-14 協和発酵バイオ株式会社 Method for culturing human pluripotent stem cells
GB201700504D0 (en) * 2017-01-11 2017-02-22 Francis Crick Inst Ltd Composition
CN112920995A (en) * 2021-03-31 2021-06-08 赵峻岭 Mesenchymal stem cell culture serum refueling bag and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020160455A1 (en) * 1999-08-25 2002-10-31 Immunex Corporation Methods and host cells for improved cell culture
US20050037492A1 (en) * 2000-01-11 2005-02-17 Chunhui Xu Medium for growing human embryonic stem cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020160455A1 (en) * 1999-08-25 2002-10-31 Immunex Corporation Methods and host cells for improved cell culture
US20050037492A1 (en) * 2000-01-11 2005-02-17 Chunhui Xu Medium for growing human embryonic stem cells

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9404079B2 (en) 2005-08-29 2016-08-02 Technion Research & Development Foundation Limited Media for culturing stem cells
US9132226B2 (en) 2008-11-14 2015-09-15 Viacyte, Inc. Encapsulation of pancreatic cells derived from human pluripotent stem cells
US9018010B2 (en) 2009-11-12 2015-04-28 Technion Research & Development Foundation Limited Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state

Also Published As

Publication number Publication date
WO2007002210A2 (en) 2007-01-04

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