WO2007002210A3 - Embryonic stem cell culture compositions and methods of use thereof - Google Patents
Embryonic stem cell culture compositions and methods of use thereof Download PDFInfo
- Publication number
- WO2007002210A3 WO2007002210A3 PCT/US2006/024168 US2006024168W WO2007002210A3 WO 2007002210 A3 WO2007002210 A3 WO 2007002210A3 US 2006024168 W US2006024168 W US 2006024168W WO 2007002210 A3 WO2007002210 A3 WO 2007002210A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- methods
- cell culture
- stem cell
- embryonic stem
- culture compositions
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/105—Insulin-like growth factors [IGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
Abstract
The present invention provides compositions and methods for the culture and maintenance of undifferentiated, pluripotent mammalian stem cells. More particularly, the present invention provides a defined media useful in the absence of a feeder layer and in the absence of serum or serum replacement, that comprises an activator of IGF-IR, an activator of an FGF receptor, serum albumin, transferrin, and optionally, a member of the TGF-β family.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US69218605P | 2005-06-20 | 2005-06-20 | |
US60/692,186 | 2005-06-20 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2007002210A2 WO2007002210A2 (en) | 2007-01-04 |
WO2007002210A3 true WO2007002210A3 (en) | 2007-07-05 |
Family
ID=37595805
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2006/024168 WO2007002210A2 (en) | 2005-06-20 | 2006-06-20 | Embryonic stem cell culture compositions and methods of use thereof |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2007002210A2 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9018010B2 (en) | 2009-11-12 | 2015-04-28 | Technion Research & Development Foundation Limited | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
US9132226B2 (en) | 2008-11-14 | 2015-09-15 | Viacyte, Inc. | Encapsulation of pancreatic cells derived from human pluripotent stem cells |
US9404079B2 (en) | 2005-08-29 | 2016-08-02 | Technion Research & Development Foundation Limited | Media for culturing stem cells |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050266554A1 (en) | 2004-04-27 | 2005-12-01 | D Amour Kevin A | PDX1 expressing endoderm |
US7541185B2 (en) | 2003-12-23 | 2009-06-02 | Cythera, Inc. | Methods for identifying factors for differentiating definitive endoderm |
US8647873B2 (en) | 2004-04-27 | 2014-02-11 | Viacyte, Inc. | PDX1 expressing endoderm |
DK2722387T3 (en) | 2003-12-23 | 2020-01-20 | Viacyte Inc | Definitely endoderm |
US7625753B2 (en) | 2003-12-23 | 2009-12-01 | Cythera, Inc. | Expansion of definitive endoderm cells |
US7985585B2 (en) | 2004-07-09 | 2011-07-26 | Viacyte, Inc. | Preprimitive streak and mesendoderm cells |
WO2007059007A2 (en) | 2005-11-14 | 2007-05-24 | Cythera, Inc. | Markers of definitive endoderm |
AU2005272681B2 (en) | 2004-08-13 | 2009-11-19 | University Of Georgia Research Foundation, Inc. | Compositions and methods for self-renewal and differentiation in human embryonic stem cells |
FI20055398A0 (en) | 2005-07-08 | 2005-07-08 | Suomen Punainen Risti Veripalv | Method for evaluating cell populations |
ES2687233T3 (en) | 2005-10-27 | 2018-10-24 | Viacyte, Inc. | Endoderm of the dorsal and ventral proximal intestine expressing PDX-1 |
US8153429B2 (en) | 2006-02-23 | 2012-04-10 | Viacyte, Inc. | Compositions and methods useful for culturing differentiable cells |
US11254916B2 (en) | 2006-03-02 | 2022-02-22 | Viacyte, Inc. | Methods of making and using PDX1-positive pancreatic endoderm cells |
US7695965B2 (en) | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
SG170068A1 (en) | 2006-03-02 | 2011-04-29 | Cythera Inc | Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production |
US9040297B2 (en) | 2006-08-02 | 2015-05-26 | Technion Research & Development Foundation Limited | Methods of expanding embryonic stem cells in a suspension culture |
EP2188369A4 (en) * | 2007-09-04 | 2011-12-07 | Univ Queensland | A feeder cell-free culture medium and system |
US7695963B2 (en) | 2007-09-24 | 2010-04-13 | Cythera, Inc. | Methods for increasing definitive endoderm production |
US20100278788A1 (en) * | 2008-01-11 | 2010-11-04 | Bone Therapeutics, S.A. | Osteogenic Differentiation Of Bone Marrow Stem Cells And Mesenchymal Stem Cells Using A Combination Of Growth Factors |
US8008075B2 (en) | 2008-11-04 | 2011-08-30 | Viacyte, Inc. | Stem cell aggregate suspension compositions and methods of differentiation thereof |
US8895300B2 (en) | 2008-11-04 | 2014-11-25 | Viacyte, Inc. | Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof |
US9109245B2 (en) | 2009-04-22 | 2015-08-18 | Viacyte, Inc. | Cell compositions derived from dedifferentiated reprogrammed cells |
JP6148429B2 (en) * | 2011-01-31 | 2017-06-14 | 協和発酵バイオ株式会社 | Method for culturing human pluripotent stem cells |
GB201700504D0 (en) * | 2017-01-11 | 2017-02-22 | Francis Crick Inst Ltd | Composition |
CN112920995A (en) * | 2021-03-31 | 2021-06-08 | 赵峻岭 | Mesenchymal stem cell culture serum refueling bag and application thereof |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020160455A1 (en) * | 1999-08-25 | 2002-10-31 | Immunex Corporation | Methods and host cells for improved cell culture |
US20050037492A1 (en) * | 2000-01-11 | 2005-02-17 | Chunhui Xu | Medium for growing human embryonic stem cells |
-
2006
- 2006-06-20 WO PCT/US2006/024168 patent/WO2007002210A2/en active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020160455A1 (en) * | 1999-08-25 | 2002-10-31 | Immunex Corporation | Methods and host cells for improved cell culture |
US20050037492A1 (en) * | 2000-01-11 | 2005-02-17 | Chunhui Xu | Medium for growing human embryonic stem cells |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9404079B2 (en) | 2005-08-29 | 2016-08-02 | Technion Research & Development Foundation Limited | Media for culturing stem cells |
US9132226B2 (en) | 2008-11-14 | 2015-09-15 | Viacyte, Inc. | Encapsulation of pancreatic cells derived from human pluripotent stem cells |
US9018010B2 (en) | 2009-11-12 | 2015-04-28 | Technion Research & Development Foundation Limited | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
Also Published As
Publication number | Publication date |
---|---|
WO2007002210A2 (en) | 2007-01-04 |
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