WO2007018744A3 - Cell-free biosynthesis of nucleic acid - Google Patents

Cell-free biosynthesis of nucleic acid Download PDF

Info

Publication number
WO2007018744A3
WO2007018744A3 PCT/US2006/023439 US2006023439W WO2007018744A3 WO 2007018744 A3 WO2007018744 A3 WO 2007018744A3 US 2006023439 W US2006023439 W US 2006023439W WO 2007018744 A3 WO2007018744 A3 WO 2007018744A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
bacterial
free
end product
dna
Prior art date
Application number
PCT/US2006/023439
Other languages
French (fr)
Other versions
WO2007018744A2 (en
Inventor
Yin Chen
Frederic Kendirgi
Frank Vazquez
Malcolm Skolnick
Original Assignee
Cytogenix Inc
Yin Chen
Frederic Kendirgi
Frank Vazquez
Malcolm Skolnick
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2005/045028 external-priority patent/WO2006063355A2/en
Application filed by Cytogenix Inc, Yin Chen, Frederic Kendirgi, Frank Vazquez, Malcolm Skolnick filed Critical Cytogenix Inc
Publication of WO2007018744A2 publication Critical patent/WO2007018744A2/en
Publication of WO2007018744A3 publication Critical patent/WO2007018744A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

Abstract

The invention provides for a process to make high quality DNA in a cell-free system, free of bacteria contaminants, and optimally free of flanking bacterial gene coding sequences which can minimize or silence gene expression when used for expression inside a target cell. The cell-free system herein is a rapid method that produces a high fidelity, cleaner end product suitable for therapeutic applications with less effort and expense, and can be adapted to amplification of plasmid-like templates lacking unnecessary bacterial plasmid gene sequences. This increases efficiency of the system and increases the effectiveness of the end product expression vector. The end product can be easily used as a DNA therapeutic due to low incidence of bacterial cell components and bacterial toxins. The invention also provides a method for the production of DNA for any research or therapeutic purpose that is essentially free of inherent bacterial cell contaminants and/or bacterial toxins, as well as for the end product therapeutics including DNA vaccines.
PCT/US2006/023439 2005-08-03 2006-06-14 Cell-free biosynthesis of nucleic acid WO2007018744A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US70534805P 2005-08-04 2005-08-04
US60/705,348 2005-08-04
USPCTUS22000/045028 2005-12-12
PCT/US2005/045028 WO2006063355A2 (en) 2004-12-11 2005-12-12 Cell free biosynthesis of high-quality nucleic acid and uses thereof
US76217206P 2006-01-25 2006-01-25
US60/762,172 2006-01-25

Publications (2)

Publication Number Publication Date
WO2007018744A2 WO2007018744A2 (en) 2007-02-15
WO2007018744A3 true WO2007018744A3 (en) 2008-07-03

Family

ID=37728257

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/023439 WO2007018744A2 (en) 2005-08-03 2006-06-14 Cell-free biosynthesis of nucleic acid

Country Status (1)

Country Link
WO (1) WO2007018744A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100008939A1 (en) * 2008-07-09 2010-01-14 General Electric Company Unprocessed rolling circle amplification product
US9125845B2 (en) 2008-07-09 2015-09-08 General Electric Company DNA vaccines, uses for unprocessed rolling circle amplification product and methods for making the same
US8921072B2 (en) 2008-09-02 2014-12-30 General Electric Compnay Methods to generate DNA mini-circles
AU2020351204A1 (en) 2019-09-18 2022-04-21 Intergalactic Therapeutics, Inc. Synthetic DNA vectors and methods of use

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030190608A1 (en) * 1999-11-12 2003-10-09 Gary Blackburn Microfluidic devices comprising biochannels
WO2005003389A2 (en) * 2003-06-28 2005-01-13 Royal Holloway And Bedford New College In vitro amplification of dna
WO2006063355A2 (en) * 2004-12-11 2006-06-15 Cytogenix , Inc. Cell free biosynthesis of high-quality nucleic acid and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030190608A1 (en) * 1999-11-12 2003-10-09 Gary Blackburn Microfluidic devices comprising biochannels
WO2005003389A2 (en) * 2003-06-28 2005-01-13 Royal Holloway And Bedford New College In vitro amplification of dna
WO2006063355A2 (en) * 2004-12-11 2006-06-15 Cytogenix , Inc. Cell free biosynthesis of high-quality nucleic acid and uses thereof

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CAMPÀS ET AL: "DNA biochip arraying, detection and amplification strategies", TRENDS IN ANALYTICAL CHEMISTRY, vol. 23, January 2004 (2004-01-01), pages 49 - 62, XP004484688 *
DEAN ET AL: "Rapid amplification of plasmid and phage DNA using Phi29 DNA polymerase and multiply-primed rolling circle amplification", GENOME RESEARCH, vol. 11, 2001, pages 1095 - 1099, XP002261245 *
DEMIDOV: "10 years of rolling the minicircles: RCA assays in DNA diagnostics", EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, vol. 5, July 2005 (2005-07-01), pages 477 - 478, XP009074526 *
LUTHRA ET AL: "Isothermal multiple displacement amplification. A highly reliable approach for generating unlimited high molecular weight genomic DNA from clinical specimens", JOURNAL OF MOLECULAR DIAGNOSTICS, vol. 6, August 2004 (2004-08-01), pages 236 - 242, XP002371240 *
RECTOR ET AL: "A sequence-independent strategy for detection and cloning of circular DNA virus genomes by using multiply primed rolling-circle amplification", JOURNAL OF VIROLOGY, vol. 78, May 2004 (2004-05-01), pages 4993 - 4998, XP002392144 *
WANG ET AL: "DNA amplification method tolerant to sample degradation", GENOME RESEARCH, vol. 14, November 2004 (2004-11-01), pages 2357 - 2366, XP002391762 *

Also Published As

Publication number Publication date
WO2007018744A2 (en) 2007-02-15

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