WO2007020457A3 - Labelled modified guanine- containing nucleosides and nucleotides comprising a fluorophore attached to the base through a linking group comprising a polyethylene glycol spacing group and methods for their use - Google Patents

Labelled modified guanine- containing nucleosides and nucleotides comprising a fluorophore attached to the base through a linking group comprising a polyethylene glycol spacing group and methods for their use Download PDF

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Publication number
WO2007020457A3
WO2007020457A3 PCT/GB2006/003095 GB2006003095W WO2007020457A3 WO 2007020457 A3 WO2007020457 A3 WO 2007020457A3 GB 2006003095 W GB2006003095 W GB 2006003095W WO 2007020457 A3 WO2007020457 A3 WO 2007020457A3
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Prior art keywords
nucleotides
methods
group
polyethylene glycol
base
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PCT/GB2006/003095
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French (fr)
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WO2007020457A2 (en
Inventor
Xiaohai Liu
John Milton
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Solexa Ltd
Xiaohai Liu
John Milton
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Application filed by Solexa Ltd, Xiaohai Liu, John Milton filed Critical Solexa Ltd
Priority to EP06779167.3A priority Critical patent/EP1926829B2/en
Publication of WO2007020457A2 publication Critical patent/WO2007020457A2/en
Publication of WO2007020457A3 publication Critical patent/WO2007020457A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Abstract

Modified guanine-containing nucleosides and nucleotides, in particular fluorescently labelled guanine-containing nucleosides and nucleotides, which exhibit reduced quenching effects, and hence enhanced brightness of the fluorophore are described.
PCT/GB2006/003095 2005-08-19 2006-08-18 Labelled modified guanine- containing nucleosides and nucleotides comprising a fluorophore attached to the base through a linking group comprising a polyethylene glycol spacing group and methods for their use WO2007020457A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP06779167.3A EP1926829B2 (en) 2005-08-19 2006-08-18 Labelled modified guanine-containing nucleosides and nucleotides comprising a fluorophore attached to the base through a linking group comprising a polyethylene glycol spacing group and methods for their use

Applications Claiming Priority (2)

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GBGB0517097.2A GB0517097D0 (en) 2005-08-19 2005-08-19 Modified nucleosides and nucleotides and uses thereof
GB0517097.2 2005-08-19

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WO2007020457A2 WO2007020457A2 (en) 2007-02-22
WO2007020457A3 true WO2007020457A3 (en) 2007-10-25

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US (3) US7592435B2 (en)
EP (1) EP1926829B2 (en)
GB (1) GB0517097D0 (en)
WO (1) WO2007020457A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11959138B2 (en) 2022-07-18 2024-04-16 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels

Families Citing this family (95)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
DE10259374A1 (en) * 2002-12-18 2004-07-08 Atto-Tec Gmbh Carboxamide-substituted dyes for analytical applications
GB0507835D0 (en) * 2005-04-18 2005-05-25 Solexa Ltd Method and device for nucleic acid sequencing using a planar wave guide
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US8997255B2 (en) 2006-07-31 2015-03-31 Inside Secure Verifying data integrity in a data storage device
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
EP2940029B1 (en) * 2007-10-19 2023-11-29 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
EP2725107B1 (en) 2007-10-19 2018-08-29 The Trustees of Columbia University in the City of New York DNA sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified ddNTPs and nucleic acid comprising inosine with reversible terminators
US8017338B2 (en) 2007-11-20 2011-09-13 Life Technologies Corporation Reversible di-nucleotide terminator sequencing
FR2927901B1 (en) * 2008-02-21 2010-09-03 Centre Nat Rech Scient PROCESS FOR THE PREPARATION OF NUCLEOTIDES AND ANALOGUES SUCH AS SYNTHESIS ON SOLUBLE CARRIER AND PREPARED BIOLOGICAL TOOLS
MX2010013678A (en) 2008-06-11 2011-02-24 Lasergen Inc Nucleotides and nucleosides and methods for their use in dna sequencing.
US9422232B2 (en) 2009-07-09 2016-08-23 The Board Of Trustees Of The Leland Stanford Junior University Reductive release probes containing a chemoselectively cleavable α-azidoether linker and methods of use thereof
WO2011130371A1 (en) 2010-04-13 2011-10-20 Life Technologies Corporation Compositions and methods for inhibition of nucleic acids function
US8759038B2 (en) 2010-09-29 2014-06-24 Illumina Cambridge Limited Compositions and methods for sequencing nucleic acids
EP2632593B1 (en) 2010-10-27 2021-09-29 Illumina, Inc. Flow cells for biological or chemical analysis
WO2013082164A1 (en) 2011-11-28 2013-06-06 Life Technologies Corporation Enhanced ligation reactions
US8883764B1 (en) 2011-01-19 2014-11-11 Illumina, Inc. Formylpyrrole-based heterocycles for nucleic acid attachment to supports
EP3670523B1 (en) 2011-09-13 2021-08-11 Agilent Technologies, Inc. 3'-oh unblocked, fast photocleavable terminating nucleotides and their use in methods for nucleic acid sequencing
DK3623481T3 (en) 2011-09-23 2021-11-15 Illumina Inc COMPOSITIONS FOR NUCLEIC ACID SEQUENCE
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
US9650406B2 (en) 2013-02-28 2017-05-16 Centrillion Technology Holdings Corporation Reversible terminator molecules and methods of their use
US8754244B1 (en) 2013-03-08 2014-06-17 Illumina Cambridge Limited Rhodamine compounds and their use as fluorescent labels
CN105263918B (en) 2013-03-08 2018-05-18 伊鲁米纳剑桥有限公司 Rhodamine compound and its purposes as fluorescent marker
CN105164106B (en) 2013-03-08 2018-04-03 伊鲁米纳剑桥有限公司 Polymethine compound and its purposes as fluorescent marker
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
DK2970356T3 (en) 2013-03-15 2018-08-27 Illumina Cambridge Ltd Modified nucleosides or nucleotides
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US9845493B2 (en) 2013-04-19 2017-12-19 Agency For Science, Technology And Research Tunable fluorescence using cleavable linkers
SG10201804913YA (en) 2013-12-10 2018-07-30 Illumina Inc Biosensors for biological or chemical analysis and methods of manufacturing the same
GB201408077D0 (en) 2014-05-07 2014-06-18 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
DK3152320T3 (en) 2014-06-03 2021-01-11 Illumina Inc Compositions, systems and methods for detecting events using tethers anchored to or adjacent to nanopores
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
WO2016065248A1 (en) 2014-10-23 2016-04-28 Complete Genomics, Inc. Signal confinement sequencing (scs) and nucleotide analogues for signal confinement sequencing
GB201508858D0 (en) 2015-05-22 2015-07-01 Illumina Cambridge Ltd Polymethine compounds with long stokes shifts and their use as fluorescent labels
IL293410B2 (en) 2015-06-03 2024-01-01 Illumina Inc Compositions, Systems, and Methods for Sequencing Polynucleotides using Tethers Anchored to Polymerases Adjacent to Nanopores
CN107849600A (en) 2015-06-09 2018-03-27 生命技术公司 For the method for molecular labeling, system, composition, kit, device and computer-readable media
US10869940B2 (en) * 2015-06-12 2020-12-22 The Board Of Trustees Of The Leland Stanford Junior University Targeted photoacoustic compounds, formulations, and uses thereof
GB201516987D0 (en) * 2015-09-25 2015-11-11 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
WO2017185026A1 (en) * 2016-04-22 2017-10-26 Complete Genomics, Inc. Reversibly blocked nucleoside analogues and their use
EP3464628B1 (en) 2016-06-06 2024-01-31 RedVault Biosciences, LP Target reporter constructs and uses thereof
US10385214B2 (en) 2016-09-30 2019-08-20 Illumina Cambridge Limited Fluorescent dyes and their uses as biomarkers
IL290157B2 (en) 2016-10-14 2023-09-01 Illumina Inc Cartridge assembly
CN109476674B (en) 2016-12-22 2021-12-10 伊鲁米纳剑桥有限公司 Coumarin compound and application thereof as fluorescent marker
WO2018121587A1 (en) * 2016-12-27 2018-07-05 深圳华大生命科学研究院 Single fluorescent dye based sequencing method
US11542540B2 (en) 2017-06-16 2023-01-03 Life Technologies Corporation Control nucleic acids, and compositions, kits, and uses thereof
GB201711219D0 (en) 2017-07-12 2017-08-23 Illumina Cambridge Ltd Short pendant arm linkers for nucleotides in sequencing applications
AU2019205797A1 (en) 2018-01-08 2019-12-19 Illumina, Inc. Systems and devices for high-throughput sequencing with semiconductor-based detection
WO2019136376A1 (en) 2018-01-08 2019-07-11 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
WO2020178231A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. Multiplexed fluorescent detection of analytes
SG11202012555UA (en) 2019-03-01 2021-01-28 Illumina Cambridge Ltd Exocyclic amine-substituted coumarin compounds and their uses as fluorescent labels
US20200277670A1 (en) 2019-03-01 2020-09-03 Illumina Cambridge Limited Tertiary amine substituted coumarin compounds and uses as fluorescent labels
NL2023327B1 (en) 2019-03-01 2020-09-17 Illumina Inc Multiplexed fluorescent detection of analytes
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
US11783917B2 (en) 2019-03-21 2023-10-10 Illumina, Inc. Artificial intelligence-based base calling
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
WO2020191389A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
US11421271B2 (en) 2019-03-28 2022-08-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
JP2023510454A (en) 2019-11-27 2023-03-14 イルミナ ケンブリッジ リミテッド Pigments and compositions containing cyclooctatetraene
US20210265018A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Knowledge Distillation and Gradient Pruning-Based Compression of Artificial Intelligence-Based Base Caller
US20210265015A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Hardware Execution and Acceleration of Artificial Intelligence-Based Base Caller
EP4107735A2 (en) 2020-02-20 2022-12-28 Illumina, Inc. Artificial intelligence-based many-to-many base calling
WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
CA3177293A1 (en) 2020-06-22 2021-12-30 Illumina Cambridge Limited Nucleosides and nucleotides with 3' acetal blocking group
US20220033900A1 (en) 2020-07-28 2022-02-03 Illumina Cambridge Limited Substituted coumarin dyes and uses as fluorescent labels
US20220067489A1 (en) 2020-08-28 2022-03-03 Illumina, Inc. Detecting and Filtering Clusters Based on Artificial Intelligence-Predicted Base Calls
US20220195516A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
US20220195517A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Long stokes shift chromenoquinoline dyes and uses in sequencing applications
US20220195196A1 (en) 2020-12-17 2022-06-23 Illumina Cambridge Limited Alkylpyridinium coumarin dyes and uses in sequencing applications
WO2022197754A1 (en) 2021-03-16 2022-09-22 Illumina Software, Inc. Neural network parameter quantization for base calling
WO2022212180A1 (en) 2021-03-31 2022-10-06 Illumina, Inc. Artificial intelligence-based base caller with contextual awareness
US20220336054A1 (en) 2021-04-15 2022-10-20 Illumina, Inc. Deep Convolutional Neural Networks to Predict Variant Pathogenicity using Three-Dimensional (3D) Protein Structures
KR20240004502A (en) 2021-05-05 2024-01-11 일루미나 케임브리지 리미티드 Fluorescent dyes containing bis-boron fused heterocycles and their use in sequencing
WO2023278184A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Methods and systems to correct crosstalk in illumination emitted from reaction sites
KR20240027599A (en) 2021-06-29 2024-03-04 일루미나, 인코포레이티드 Self-learning base caller trained using oligo sequences
US20230005253A1 (en) 2021-07-01 2023-01-05 Illumina, Inc. Efficient artificial intelligence-based base calling of index sequences
US20230027409A1 (en) 2021-07-13 2023-01-26 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
KR20240037882A (en) 2021-07-28 2024-03-22 일루미나, 인코포레이티드 Quality score calibration of the base calling system
KR20240035413A (en) 2021-08-03 2024-03-15 일루미나, 인코포레이티드 Base calling using the multi-base caller model
WO2023049215A1 (en) 2021-09-22 2023-03-30 Illumina, Inc. Compressed state-based base calling
WO2023183937A1 (en) 2022-03-25 2023-09-28 Illumina, Inc. Sequence-to-sequence base calling
CA3223125A1 (en) 2022-03-29 2023-10-05 Illumina, Inc. Chromenoquinoline dyes and uses in sequencing
CA3222797A1 (en) 2022-03-31 2023-10-05 Ramesh NEELAKANDAN Nucleosides and nucleotides with 3' vinyl blocking group
US20230357845A1 (en) 2022-03-31 2023-11-09 Illumina, Inc. Compositions and methods for improving sequencing signals
US20230416279A1 (en) 2022-06-28 2023-12-28 Illumina Cambridge Limited Fluorescent dyes containing fused tetracyclic bis-boron heterocycle and uses in sequencing
WO2024068889A2 (en) 2022-09-30 2024-04-04 Illumina, Inc. Compositions and methods for reducing photo damage during sequencing
CN116239493A (en) * 2023-03-15 2023-06-09 杭州易速微控基因技术有限公司 Synthesis method of Linker compound

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6380378B1 (en) * 1998-12-24 2002-04-30 Toagosei Company, Ltd. Nucleotide compound, nucleotide block oligonucleotide, and method for producing them
WO2004018493A1 (en) * 2002-08-23 2004-03-04 Solexa Limited Labelled nucleotides
WO2006097320A2 (en) * 2005-03-17 2006-09-21 Genovoxx Gmbh Macromolecular nucleotide links and methods for their use
WO2007015168A2 (en) * 2005-07-01 2007-02-08 Dako Denmark A/S Monomeric and polymeric linkers useful for conjugating biological molecules and other substances

Family Cites Families (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5175269A (en) 1984-01-30 1992-12-29 Enzo Diagnostics, Inc. Compound and detectable molecules having an oligo- or polynucleotide with modifiable reactive group
US5118605A (en) 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4824775A (en) 1985-01-03 1989-04-25 Molecular Diagnostics, Inc. Cells labeled with multiple Fluorophores bound to a nucleic acid carrier
US4772691A (en) 1985-06-05 1988-09-20 The Medical College Of Wisconsin, Inc. Chemically cleavable nucleotides
US4863849A (en) 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
SE8801070D0 (en) 1988-03-23 1988-03-23 Pharmacia Ab METHOD FOR IMMOBILIZING A DNA SEQUENCE ON A SOLID SUPPORT
US5174962A (en) 1988-06-20 1992-12-29 Genomyx, Inc. Apparatus for determining DNA sequences by mass spectrometry
GB8910880D0 (en) 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
WO1993005183A1 (en) 1991-09-09 1993-03-18 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme
DE4141178A1 (en) 1991-12-13 1993-06-17 Europ Lab Molekularbiolog New nucleic acid sequencing method - using one labelled nucleotide at one time in cycles comprising elongation, wash, label detection and removal of the label, then repeating
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
GB9210176D0 (en) 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
US5516664A (en) 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
US5436143A (en) 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
US6074823A (en) 1993-03-19 2000-06-13 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
FR2703052B1 (en) 1993-03-26 1995-06-02 Pasteur Institut New method of nucleic acid sequencing.
GB9315847D0 (en) 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US5547859A (en) 1993-08-02 1996-08-20 Goodman; Myron F. Chain-terminating nucleotides for DNA sequencing methods
DE69426731T2 (en) 1993-11-17 2001-06-28 Amersham Pharm Biotech Uk Ltd METHOD FOR MASS SPECTROSCOPIC SEQUENCE ANALYSIS OF A NUCLEIC ACID BY PRIMER EXTENSION
US5872244A (en) 1994-09-02 1999-02-16 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US5763594A (en) 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US5808045A (en) 1994-09-02 1998-09-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US6214987B1 (en) 1994-09-02 2001-04-10 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent formation of phosphodiester bonds using protected nucleotides
US6232465B1 (en) 1994-09-02 2001-05-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
SE9500342D0 (en) 1995-01-31 1995-01-31 Marek Kwiatkowski Novel chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation
WO1996027025A1 (en) 1995-02-27 1996-09-06 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
EP0745686A1 (en) 1995-06-01 1996-12-04 Roche Diagnostics GmbH The use of DNA polymerase 3'-intrinsic editing activity
US6312893B1 (en) 1996-01-23 2001-11-06 Qiagen Genomics, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
EP0992511B1 (en) 1996-01-23 2009-03-11 Operon Biotechnologies, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
US6613508B1 (en) 1996-01-23 2003-09-02 Qiagen Genomics, Inc. Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques
US5821542A (en) * 1996-06-26 1998-10-13 International Business Machines Corporation Particle beam imaging system having hollow beam illumination
US5821356A (en) 1996-08-12 1998-10-13 The Perkin Elmer Corporation Propargylethoxyamino nucleotides
US5885775A (en) 1996-10-04 1999-03-23 Perseptive Biosystems, Inc. Methods for determining sequences information in polynucleotides using mass spectrometry
EP1015629A1 (en) 1997-01-08 2000-07-05 Proligo L.L.C. Bioconjugation of oligonucleotides
US6046005A (en) 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
US6136543A (en) 1997-01-31 2000-10-24 Hitachi, Ltd. Method for determining nucleic acids base sequence and apparatus therefor
PT1017848E (en) 1997-07-28 2003-02-28 Medical Biosystems Ltd ANALYSIS OF NUCLEIC ACID SEQUENCES
US6008379A (en) 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
GB9815163D0 (en) 1998-07-13 1998-09-09 Brax Genomics Ltd Compounds
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
EP1082458A1 (en) 1998-05-01 2001-03-14 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules
US6096875A (en) 1998-05-29 2000-08-01 The Perlein-Elmer Corporation Nucleotide compounds including a rigid linker
US6218530B1 (en) 1998-06-02 2001-04-17 Ambergen Inc. Compounds and methods for detecting biomolecules
US6287821B1 (en) 1998-06-11 2001-09-11 Orchid Biosciences, Inc. Nucleotide analogues with 3'-pro-fluorescent fluorophores in nucleic acid sequence analysis
US6218118B1 (en) 1998-07-09 2001-04-17 Agilent Technologies, Inc. Method and mixture reagents for analyzing the nucleotide sequence of nucleic acids by mass spectrometry
WO2000006770A1 (en) 1998-07-30 2000-02-10 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
DE19844931C1 (en) 1998-09-30 2000-06-15 Stefan Seeger Procedures for DNA or RNA sequencing
EP1163052B1 (en) 1999-02-23 2010-06-02 Caliper Life Sciences, Inc. Manipulation of microparticles in microfluidic systems
EP1159453B1 (en) 1999-03-10 2008-05-28 ASM Scientific, Inc. A method for direct nucleic acid sequencing
US7037654B2 (en) 1999-04-30 2006-05-02 Aclara Biosciences, Inc. Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6242193B1 (en) 1999-07-30 2001-06-05 Hitachi, Ltd. Apparatus for determining base sequence of nucleic acid
AU7086800A (en) 1999-08-30 2001-03-26 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The High speed parallel molecular nucleic acid sequencing
US6982146B1 (en) 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
US6309836B1 (en) 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
WO2001032930A1 (en) 1999-11-04 2001-05-10 California Institute Of Technology Methods and apparatuses for analyzing polynucleotide sequences
WO2001048184A2 (en) 1999-12-23 2001-07-05 Axaron Bioscience Ag Method for carrying out the parallel sequencing of a nucleic acid mixture on a surface
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
EP1182267B1 (en) 2000-03-30 2012-01-18 Toyota Jidosha Kabushiki Kaisha Method of determining base sequence of single nucleic acid molecule
GB0013276D0 (en) 2000-06-01 2000-07-26 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
GB0016473D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
US6613523B2 (en) 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
WO2003006625A2 (en) 2001-07-13 2003-01-23 Ambergen, Inc. Nucleotide compositions comprising photocleavable markers and methods of preparation thereof
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
EP1497304B1 (en) * 2002-04-12 2014-06-25 Catalyst Assets LLC Dual-labeled nucleotides
DE10219121A1 (en) 2002-04-29 2003-11-27 Infineon Technologies Ag Silicon particles as additives to improve charge carrier mobility in organic semiconductors
US7074597B2 (en) 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
EP3002289B1 (en) 2002-08-23 2018-02-28 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
EP1546371A1 (en) † 2002-10-03 2005-06-29 Ecole Polytechnique Federale De Lausanne (Epfl) Substrates for o6-alkylguanine-dna alkyltransferase
EP1565480B1 (en) † 2002-11-22 2018-08-22 Roche Diagnostics GmbH Detectable labeled nucleoside analogs and methods of use thereof
WO2005044836A2 (en) † 2003-11-05 2005-05-19 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
EP2436778A3 (en) 2004-03-03 2012-07-11 The Trustees of Columbia University in the City of New York Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6380378B1 (en) * 1998-12-24 2002-04-30 Toagosei Company, Ltd. Nucleotide compound, nucleotide block oligonucleotide, and method for producing them
WO2004018493A1 (en) * 2002-08-23 2004-03-04 Solexa Limited Labelled nucleotides
WO2006097320A2 (en) * 2005-03-17 2006-09-21 Genovoxx Gmbh Macromolecular nucleotide links and methods for their use
WO2007015168A2 (en) * 2005-07-01 2007-02-08 Dako Denmark A/S Monomeric and polymeric linkers useful for conjugating biological molecules and other substances

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KURATA S ET AL: "Fluorescent quenching-based quantitative detection of specific DNA/RNA using a BODIPY((R)) FL-labeled probe or primer", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 29, no. 6, 15 March 2001 (2001-03-15), pages E34, XP002376104, ISSN: 0305-1048 *
TORIMURA M ET AL: "FLUORESCENCE-QUENCHING PHENOMENON BY PHOTOINDUCED ELECTRON TRANSFER BETWEEN A FLUORESCENT DYE AND A NUCLEOTIDE BASE", ANALYTICAL SCIENCES, JAPAN SOCIETY FOR ANALYTICAL CHEMISTRY, TOKYO,, JP, vol. 17, no. 1, January 2001 (2001-01-01), pages 155 - 160, XP002953171, ISSN: 0910-6340 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11959138B2 (en) 2022-07-18 2024-04-16 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels

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US7816503B2 (en) 2010-10-19
US8212015B2 (en) 2012-07-03
US20110020827A1 (en) 2011-01-27
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