WO2007044025A3 - Method for detecting analytes based on evanescent illumination and scatter-based detection of nonoparticle probe complexes - Google Patents

Method for detecting analytes based on evanescent illumination and scatter-based detection of nonoparticle probe complexes Download PDF

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Publication number
WO2007044025A3
WO2007044025A3 PCT/US2005/042326 US2005042326W WO2007044025A3 WO 2007044025 A3 WO2007044025 A3 WO 2007044025A3 US 2005042326 W US2005042326 W US 2005042326W WO 2007044025 A3 WO2007044025 A3 WO 2007044025A3
Authority
WO
WIPO (PCT)
Prior art keywords
nonoparticle
scatter
detecting analytes
probe complexes
evanescent illumination
Prior art date
Application number
PCT/US2005/042326
Other languages
French (fr)
Other versions
WO2007044025A9 (en
WO2007044025A2 (en
Inventor
James J Storhoff
Adam Lucas
Uwe R Mueller
Yijia Paul Bao
Michael Senical
Viswanadham Garimella
Original Assignee
Nanosphere Inc
James J Storhoff
Adam Lucas
Uwe R Mueller
Yijia Paul Bao
Michael Senical
Viswanadham Garimella
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/995,051 external-priority patent/US20050250094A1/en
Application filed by Nanosphere Inc, James J Storhoff, Adam Lucas, Uwe R Mueller, Yijia Paul Bao, Michael Senical, Viswanadham Garimella filed Critical Nanosphere Inc
Publication of WO2007044025A2 publication Critical patent/WO2007044025A2/en
Publication of WO2007044025A9 publication Critical patent/WO2007044025A9/en
Publication of WO2007044025A3 publication Critical patent/WO2007044025A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Abstract

The invention provides methods of detecting one or more specific binding analytes, such as nucleic acids and proteins, in the presence of a neutral or anionic polysaccharide, through light scattering techniques, where a change in light scattering caused by the formation of nanoparticle label complexes within the penetration depth of the evanescent wave of a wave guide signals the presence of the analyte.
PCT/US2005/042326 2004-11-22 2005-11-21 Method for detecting analytes based on evanescent illumination and scatter-based detection of nonoparticle probe complexes WO2007044025A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/995,051 2004-11-22
US10/995,051 US20050250094A1 (en) 2003-05-30 2004-11-22 Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes

Publications (3)

Publication Number Publication Date
WO2007044025A2 WO2007044025A2 (en) 2007-04-19
WO2007044025A9 WO2007044025A9 (en) 2007-06-28
WO2007044025A3 true WO2007044025A3 (en) 2007-08-09

Family

ID=37943235

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/042326 WO2007044025A2 (en) 2004-11-22 2005-11-21 Method for detecting analytes based on evanescent illumination and scatter-based detection of nonoparticle probe complexes

Country Status (1)

Country Link
WO (1) WO2007044025A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180258473A1 (en) 2015-11-10 2018-09-13 Administracion General De La Comunidad Autonoma De Euskadi Diagnostic methods and devices
CN115014886A (en) * 2021-03-05 2022-09-06 希森美康株式会社 Method for preparing measurement sample, method for analyzing measurement sample, reagent, and kit

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6506564B1 (en) * 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
WO2003029435A2 (en) * 2001-10-04 2003-04-10 The Regents Of The University Of California Detection of polynucleotide hybridization
WO2004053105A2 (en) * 2002-12-12 2004-06-24 Nanosphere, Inc. Direct snp detection with unamplified dna
WO2005001143A2 (en) * 2003-02-27 2005-01-06 Nanosphere, Inc. Label-free gene expression profiling with universal nanoparticle probes in microarray assay format
WO2005008222A2 (en) * 2003-05-30 2005-01-27 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes
US20050250094A1 (en) * 2003-05-30 2005-11-10 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6506564B1 (en) * 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
WO2003029435A2 (en) * 2001-10-04 2003-04-10 The Regents Of The University Of California Detection of polynucleotide hybridization
WO2004053105A2 (en) * 2002-12-12 2004-06-24 Nanosphere, Inc. Direct snp detection with unamplified dna
WO2005001143A2 (en) * 2003-02-27 2005-01-06 Nanosphere, Inc. Label-free gene expression profiling with universal nanoparticle probes in microarray assay format
WO2005008222A2 (en) * 2003-05-30 2005-01-27 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes
US20050250094A1 (en) * 2003-05-30 2005-11-10 Nanosphere, Inc. Method for detecting analytes based on evanescent illumination and scatter-based detection of nanoparticle probe complexes

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ELGHANIAN ET AL: "Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 277, 27 August 1997 (1997-08-27), pages 1078 - 1081, XP002294466, ISSN: 0036-8075 *
LIU TAO ET AL: "Sensitivity enhancement of DNA sensors by nanogold surface modification", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 295, no. 1, July 2002 (2002-07-01), pages 14 - 16, XP002313771, ISSN: 0006-291X *
MIRKIN C A ET AL: "A DNA-BASED METHOD FOR RATIONALLY ASSEMBLING NANOPARTICLES INTO MACROSCOPIC MATERIALS", NATURE, NATURE PUBLISHING GROUP, LONDON, GB, vol. 382, no. 6592, 15 August 1996 (1996-08-15), pages 609 - 611, XP001092721, ISSN: 0028-0836 *
STORHOFF J J ET AL: "One-pot colorimetric differentiation of polynucleotides with single base imperfection using gold nanoparticle probes", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 120, 11 March 1998 (1998-03-11), pages 1959 - 1964, XP002113277, ISSN: 0002-7863 *
STORHOFF J J ET AL: "What controls the optical properties of DNA-linked gold nanoparticle assemblies?", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 122, no. 19, 17 May 2000 (2000-05-17), pages 4640 - 4650, XP002313770, ISSN: 0002-7863 *

Also Published As

Publication number Publication date
WO2007044025A9 (en) 2007-06-28
WO2007044025A2 (en) 2007-04-19

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