WO2007049264A3 - Assays using nanoparticles - Google Patents

Assays using nanoparticles Download PDF

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Publication number
WO2007049264A3
WO2007049264A3 PCT/IE2006/000123 IE2006000123W WO2007049264A3 WO 2007049264 A3 WO2007049264 A3 WO 2007049264A3 IE 2006000123 W IE2006000123 W IE 2006000123W WO 2007049264 A3 WO2007049264 A3 WO 2007049264A3
Authority
WO
WIPO (PCT)
Prior art keywords
nanoparticles
buffered
assays
test sample
solution
Prior art date
Application number
PCT/IE2006/000123
Other languages
French (fr)
Other versions
WO2007049264A2 (en
Inventor
Yuri Volkov
Yury Rakovich
Iouri Kuzmich Gounko
John Dongegan
Dermot Kelleher
Siobhan Mitchell
Original Assignee
Trinity College Dublin
Yuri Volkov
Yury Rakovich
Iouri Kuzmich Gounko
John Dongegan
Dermot Kelleher
Siobhan Mitchell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Trinity College Dublin, Yuri Volkov, Yury Rakovich, Iouri Kuzmich Gounko, John Dongegan, Dermot Kelleher, Siobhan Mitchell filed Critical Trinity College Dublin
Priority to US12/084,239 priority Critical patent/US20090197291A1/en
Publication of WO2007049264A2 publication Critical patent/WO2007049264A2/en
Publication of WO2007049264A3 publication Critical patent/WO2007049264A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/588—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with semiconductor nanocrystal label, e.g. quantum dots
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery

Abstract

A method for quantitatively and qualitatively determining the presence of a macromolecule comprises providing nanoparticles in a buffered solution, adding a test sample to the buffered nanoparticle solution, and measuring the difference between the buffered nanoparticles in the presence and absence of the test sample. The nanoparticles are preferably less than 100 nm in size.
PCT/IE2006/000123 2005-10-27 2006-10-27 Assays using nanoparticles WO2007049264A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/084,239 US20090197291A1 (en) 2005-10-27 2006-10-27 Assays Using Nanoparticles

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US73036505P 2005-10-27 2005-10-27
US60/730,365 2005-10-27

Publications (2)

Publication Number Publication Date
WO2007049264A2 WO2007049264A2 (en) 2007-05-03
WO2007049264A3 true WO2007049264A3 (en) 2007-07-19

Family

ID=37714424

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IE2006/000123 WO2007049264A2 (en) 2005-10-27 2006-10-27 Assays using nanoparticles

Country Status (1)

Country Link
WO (1) WO2007049264A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6114038A (en) * 1998-11-10 2000-09-05 Biocrystal Ltd. Functionalized nanocrystals and their use in detection systems
US6306610B1 (en) * 1998-09-18 2001-10-23 Massachusetts Institute Of Technology Biological applications of quantum dots
US6468808B1 (en) * 1998-09-24 2002-10-22 Advanced Research And Technology Institute, Inc. Water-soluble luminescent quantum dots and biomolecular conjugates thereof and related compositions and method of use

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306610B1 (en) * 1998-09-18 2001-10-23 Massachusetts Institute Of Technology Biological applications of quantum dots
US6468808B1 (en) * 1998-09-24 2002-10-22 Advanced Research And Technology Institute, Inc. Water-soluble luminescent quantum dots and biomolecular conjugates thereof and related compositions and method of use
US6114038A (en) * 1998-11-10 2000-09-05 Biocrystal Ltd. Functionalized nanocrystals and their use in detection systems

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LI ET AL: "Application of L-cysteine-capped ZnS nanoparticles in the determination of nucleic acid using teh resonance light scattering method", MICROCHIMICA ACTA, vol. 146, 8 March 2004 (2004-03-08), pages 13 - 19, XP002419963 *
ZHU CHANG QING ET AL: "Application of L-cysteine-capped nano-ZnS as a fluorescence probe for the determination of proteins.", ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol. 378, no. 3, February 2004 (2004-02-01), pages 811 - 815, XP002419964, ISSN: 1618-2642 *

Also Published As

Publication number Publication date
WO2007049264A2 (en) 2007-05-03

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