WO2007070381A3 - Nucleic acid purification with a binding matrix - Google Patents

Nucleic acid purification with a binding matrix Download PDF

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Publication number
WO2007070381A3
WO2007070381A3 PCT/US2006/046879 US2006046879W WO2007070381A3 WO 2007070381 A3 WO2007070381 A3 WO 2007070381A3 US 2006046879 W US2006046879 W US 2006046879W WO 2007070381 A3 WO2007070381 A3 WO 2007070381A3
Authority
WO
WIPO (PCT)
Prior art keywords
binding matrix
dna
nucleic acid
rna
acid purification
Prior art date
Application number
PCT/US2006/046879
Other languages
French (fr)
Other versions
WO2007070381A2 (en
Inventor
Rex M Bitner
Paula R Brisco
Michelle N Mandrekar
Original Assignee
Promega Corp
Rex M Bitner
Paula R Brisco
Michelle N Mandrekar
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp, Rex M Bitner, Paula R Brisco, Michelle N Mandrekar filed Critical Promega Corp
Priority to EP06845029A priority Critical patent/EP1963526A4/en
Publication of WO2007070381A2 publication Critical patent/WO2007070381A2/en
Publication of WO2007070381A3 publication Critical patent/WO2007070381A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

The present invention relates to methods, kits, and compositions for generating purified RNA samples and purified DNA samples. In particular, the present invention provides methods for generating a purified RNA or DNA sample from a sample containing both DNA and RNA molecules using a binding matrix that preferentially binds DNA or RNA in the presence of an acidic dilution buffer, or using a binding matrix that comprises acid zeolites, as well as compositions and kits for practicing such methods.
PCT/US2006/046879 2005-12-09 2006-12-08 Nucleic acid purification with a binding matrix WO2007070381A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP06845029A EP1963526A4 (en) 2005-12-09 2006-12-08 Nucleic acid purification with a binding matrix

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US74882505P 2005-12-09 2005-12-09
US60/748,825 2005-12-09

Publications (2)

Publication Number Publication Date
WO2007070381A2 WO2007070381A2 (en) 2007-06-21
WO2007070381A3 true WO2007070381A3 (en) 2008-04-17

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/046879 WO2007070381A2 (en) 2005-12-09 2006-12-08 Nucleic acid purification with a binding matrix

Country Status (3)

Country Link
US (2) US8030034B2 (en)
EP (1) EP1963526A4 (en)
WO (1) WO2007070381A2 (en)

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US20110046361A1 (en) * 2008-04-30 2011-02-24 Ge Healthcare Bio-Sciences Corp. Method for separation of double-stranded and single-stranded nucleic acids
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US8222397B2 (en) 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
US8039613B2 (en) * 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US9040679B2 (en) 2012-04-30 2015-05-26 General Electric Company Methods and compositions for extraction and storage of nucleic acids
US9040675B2 (en) 2012-04-30 2015-05-26 General Electric Company Formulations for nucleic acid stabilization on solid substrates
US9044738B2 (en) 2012-04-30 2015-06-02 General Electric Company Methods and compositions for extraction and storage of nucleic acids
US9480966B2 (en) 2012-04-30 2016-11-01 General Electric Company Substrates and methods for collection, stabilization and elution of biomolecules
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US11410751B2 (en) * 2016-05-27 2022-08-09 Life Technologies Corporation Methods and systems for graphical user interfaces for biological data
KR102090877B1 (en) * 2017-10-30 2020-03-18 울산대학교 산학협력단 Method for pathogen lysis and nucleic acid extract using zinc oxide nanostar
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Also Published As

Publication number Publication date
US8030034B2 (en) 2011-10-04
US20120059160A1 (en) 2012-03-08
US8658360B2 (en) 2014-02-25
WO2007070381A2 (en) 2007-06-21
EP1963526A2 (en) 2008-09-03
US20070172855A1 (en) 2007-07-26
EP1963526A4 (en) 2009-11-18

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