WO2007087570A3 - Polymerized hemoglobin media and its use in isolation and transplantation of islet cells - Google Patents

Polymerized hemoglobin media and its use in isolation and transplantation of islet cells Download PDF

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Publication number
WO2007087570A3
WO2007087570A3 PCT/US2007/060989 US2007060989W WO2007087570A3 WO 2007087570 A3 WO2007087570 A3 WO 2007087570A3 US 2007060989 W US2007060989 W US 2007060989W WO 2007087570 A3 WO2007087570 A3 WO 2007087570A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
suspensions
solutions
mammalian cells
isolation
Prior art date
Application number
PCT/US2007/060989
Other languages
French (fr)
Other versions
WO2007087570A2 (en
Inventor
Marc DOUBLEDAY
Original Assignee
Northfield Lab
Marc DOUBLEDAY
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Northfield Lab, Marc DOUBLEDAY filed Critical Northfield Lab
Priority to EP07762535A priority Critical patent/EP1978802A2/en
Priority to JP2008552556A priority patent/JP2009524436A/en
Publication of WO2007087570A2 publication Critical patent/WO2007087570A2/en
Publication of WO2007087570A3 publication Critical patent/WO2007087570A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/39—Pancreas; Islets of Langerhans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/41—Porphyrin- or corrin-ring-containing peptides
    • A61K38/42—Haemoglobins; Myoglobins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/482—Serine endopeptidases (3.4.21)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/4886—Metalloendopeptidases (3.4.24), e.g. collagenase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics

Abstract

Solutions and suspensions comprising polymerized hemoglobin derived from human blood are disclosed. The solutions and suspensions may comprise cell culture medium, an enzyme (such as a protease), and/or a buffer. Processes of preparing the solutions and suspensions are also disclosed. The solutions and suspensions may be employed in methods of isolating mammalian cells, such as pancreatic islets, methods of preserving mammalian tissue and organs, methods of aiding the recovery of mammalian cells following their isolation, methods of maintaining mammalian cells, methods of propagating mammalian cells, and methods of treating a mammal with diabetes.
PCT/US2007/060989 2006-01-24 2007-01-24 Polymerized hemoglobin media and its use in isolation and transplantation of islet cells WO2007087570A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP07762535A EP1978802A2 (en) 2006-01-24 2007-01-24 Polymerized hemoglobin media and its use in isolation and transplantation of islet cells
JP2008552556A JP2009524436A (en) 2006-01-24 2007-01-24 Polymerized hemoglobin medium and its use in isolation and transplantation of islet cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US76166306P 2006-01-24 2006-01-24
US60/761,663 2006-01-24

Publications (2)

Publication Number Publication Date
WO2007087570A2 WO2007087570A2 (en) 2007-08-02
WO2007087570A3 true WO2007087570A3 (en) 2007-12-06

Family

ID=38293339

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2007/060987 WO2008021577A2 (en) 2006-01-24 2007-01-24 Polymerized hemoglobin media and its use in isolation and transplantation of islet cells
PCT/US2007/060989 WO2007087570A2 (en) 2006-01-24 2007-01-24 Polymerized hemoglobin media and its use in isolation and transplantation of islet cells

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2007/060987 WO2008021577A2 (en) 2006-01-24 2007-01-24 Polymerized hemoglobin media and its use in isolation and transplantation of islet cells

Country Status (4)

Country Link
US (6) US20070196810A1 (en)
EP (1) EP1978802A2 (en)
JP (1) JP2009524436A (en)
WO (2) WO2008021577A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1978802A2 (en) * 2006-01-24 2008-10-15 Northfield Laboratories, Inc. Polymerized hemoglobin media and its use in isolation and transplantation of islet cells
US20090004159A1 (en) * 2006-01-24 2009-01-01 The Board Of Trustees Of The University Of Illinoi Polymerized Hemoglobin Media and Its Use in Isolation and Transplantation of Islet Cells
FR2931164B1 (en) 2008-05-16 2010-08-20 Maco Pharma Sa METHOD FOR PRESERVING INSULIN SECRETORY CELLS FOR TRANSPLANTING
FR2959754B1 (en) 2010-05-05 2012-06-08 Maco Pharma Sa BIOLOGICAL MEDIUM FOR PRESERVING PREPARATION OF INSULIN SECRETORY CELLS.
US10433538B2 (en) 2012-10-12 2019-10-08 University of Pittsburgh—of the Commonwealth System of Higher Education Compositions and methods for organ preservation
US20140227677A1 (en) * 2013-02-07 2014-08-14 OPK Biotech, LLC Perfusion of prospective donor hearts with polymerized hemoglobin

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Also Published As

Publication number Publication date
WO2008021577A2 (en) 2008-02-21
US20130316455A1 (en) 2013-11-28
WO2008021577A3 (en) 2008-10-02
US20170355961A1 (en) 2017-12-14
WO2007087570A2 (en) 2007-08-02
JP2009524436A (en) 2009-07-02
EP1978802A2 (en) 2008-10-15
US20130095467A1 (en) 2013-04-18
US9657271B2 (en) 2017-05-23
US20070196810A1 (en) 2007-08-23
US20110200981A1 (en) 2011-08-18
US20110142808A1 (en) 2011-06-16

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