WO2007130521A2 - Fermentive production of four carbon alcohols - Google Patents
Fermentive production of four carbon alcohols Download PDFInfo
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- WO2007130521A2 WO2007130521A2 PCT/US2007/010744 US2007010744W WO2007130521A2 WO 2007130521 A2 WO2007130521 A2 WO 2007130521A2 US 2007010744 W US2007010744 W US 2007010744W WO 2007130521 A2 WO2007130521 A2 WO 2007130521A2
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- WO
- WIPO (PCT)
- Prior art keywords
- seq
- amino acid
- acid sequence
- butanol
- diol
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
- C12P7/26—Ketones
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims
Priority Applications (8)
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BRPI0710412-0A BRPI0710412A2 (en) | 2006-05-02 | 2007-05-02 | "RECOMBINANT MICROBIAN HOST CELLS, METHOD FOR 2-BUTHANOL PRODUCTION, METHOD FOR 2-BUTANONE, 2-BUTANOL AND 2-BUTANONE PRODUCTION" |
JP2009509717A JP5315232B2 (en) | 2006-05-02 | 2007-05-02 | Fermentation of 4-carbon alcohol |
NZ570931A NZ570931A (en) | 2006-05-02 | 2007-05-02 | Fermentive production of four carbon alcohols |
CA002645497A CA2645497A1 (en) | 2006-05-02 | 2007-05-02 | Fermentive production of four carbon alcohols |
ES07776684.8T ES2441182T3 (en) | 2006-05-02 | 2007-05-02 | Fermentative production of four-carbon alcohols |
EP07776684.8A EP2010643B1 (en) | 2006-05-02 | 2007-05-02 | Fermentive production of four carbon alcohols |
MX2008013882A MX2008013882A (en) | 2006-05-02 | 2007-05-02 | Fermentive production of four carbon alcohols. |
AU2007248560A AU2007248560C1 (en) | 2006-05-02 | 2007-05-02 | Fermentive production of four carbon alcohols |
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US79681606P | 2006-05-02 | 2006-05-02 | |
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WO2007130521A3 WO2007130521A3 (en) | 2008-03-20 |
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PCT/US2007/010741 WO2007130518A2 (en) | 2006-05-02 | 2007-05-02 | Fermentive production of four carbon alcohols |
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PCT/US2007/010741 WO2007130518A2 (en) | 2006-05-02 | 2007-05-02 | Fermentive production of four carbon alcohols |
Country Status (12)
Country | Link |
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US (4) | US8962298B2 (en) |
EP (4) | EP2405014A1 (en) |
JP (2) | JP2009535062A (en) |
AU (2) | AU2007248557B2 (en) |
BR (2) | BRPI0710414A2 (en) |
CA (2) | CA2645497A1 (en) |
ES (1) | ES2441182T3 (en) |
MX (2) | MX2008013882A (en) |
NZ (2) | NZ570930A (en) |
RU (2) | RU2008147393A (en) |
WO (2) | WO2007130521A2 (en) |
ZA (1) | ZA200807718B (en) |
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WO2009149270A2 (en) | 2008-06-04 | 2009-12-10 | E. I. Du Pont De Nemours And Company | A method for producing butanol using two-phase extractive fermentation |
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US8017364B2 (en) * | 2006-12-12 | 2011-09-13 | Butamax(Tm) Advanced Biofuels Llc | Solvent tolerant microorganisms |
EP2094844A4 (en) * | 2006-12-15 | 2010-01-06 | Biofuelchem Co Ltd | Enhanced butanol producing microorganisms and method for preparing butanol using the same |
WO2008074794A1 (en) * | 2006-12-19 | 2008-06-26 | Dsm Ip Assets B.V. | Butanol production in a prokaryotic cell |
US8673601B2 (en) | 2007-01-22 | 2014-03-18 | Genomatica, Inc. | Methods and organisms for growth-coupled production of 3-hydroxypropionic acid |
KR100971792B1 (en) * | 2007-02-08 | 2010-07-23 | 바이오퓨얼켐 주식회사 | Method for preparing butanol through butyryl-coa as an intermediate using yeast |
LT2821494T (en) | 2007-03-16 | 2017-04-25 | Genomatica, Inc. | Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors |
KR101573775B1 (en) | 2007-04-18 | 2015-12-04 | 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 | - fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes |
US8426174B2 (en) * | 2007-05-02 | 2013-04-23 | Butamax(Tm) Advanced Biofuels Llc | Method for the production of 2-butanol |
US20080274522A1 (en) * | 2007-05-02 | 2008-11-06 | Bramucci Michael G | Method for the production of 2-butanone |
CA2693084A1 (en) * | 2007-06-08 | 2008-12-18 | Benjamin S. Bower | Heterologous and homologous cellulase expression system |
US7947483B2 (en) | 2007-08-10 | 2011-05-24 | Genomatica, Inc. | Methods and organisms for the growth-coupled production of 1,4-butanediol |
AU2008307381B2 (en) * | 2007-08-10 | 2014-02-20 | Genomatica, Inc. | Methods for the synthesis of acrylic acid and derivatives from fumaric acid |
US7819976B2 (en) | 2007-08-22 | 2010-10-26 | E. I. Du Pont De Nemours And Company | Biomass treatment method |
EP2225351A4 (en) | 2007-12-03 | 2016-11-09 | Gevo Inc | Renewable compositions |
US8193402B2 (en) | 2007-12-03 | 2012-06-05 | Gevo, Inc. | Renewable compositions |
WO2009076480A2 (en) * | 2007-12-10 | 2009-06-18 | Synthetic Genomics, Inc. | Methylbutanol as an advanced biofuel |
EP2237679A4 (en) * | 2007-12-14 | 2014-01-01 | Abengoa Bioenergy New Technologies Inc | Improved quality and value of co-products of the ethanol production industry |
MX2010006679A (en) | 2007-12-17 | 2010-11-30 | Univ Amsterdam | Light-driven co2 reduction to organic compounds to serve as fuels or as industrial half products by an autotroph containing a fermentative gene cassette. |
US20090162911A1 (en) * | 2007-12-21 | 2009-06-25 | E.I. Du Pont De Nemours And Company | Strain for butanol production |
US8518678B2 (en) * | 2007-12-21 | 2013-08-27 | Butamax(Tm) Advanced Biofuels Llc | Strain comprising increased expression of a CFA coding region for butanol production |
EP2072621A1 (en) * | 2007-12-21 | 2009-06-24 | DSM IP Assets B.V. | Solvent tolerant microorganisms |
US8372612B2 (en) * | 2007-12-21 | 2013-02-12 | Butamax(Tm) Advanced Biofuels Llc | Production of four carbon alcohols using improved strain |
CA2712779C (en) | 2008-01-22 | 2021-03-16 | Genomatica, Inc. | Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol |
SI2262901T1 (en) | 2008-03-05 | 2019-02-28 | Genomatica, Inc. | Primary alcohol producing organisms |
TWI701230B (en) * | 2008-03-11 | 2020-08-11 | 美商吉諾瑪蒂卡股份有限公司 | PREPARATION OF 6-AMINOCAPROIC ACID FROM α-KETOPIMELIC ACID |
CN103555643B (en) | 2008-03-27 | 2016-08-10 | 基因组股份公司 | For producing the microorganism of adipic acid and other compounds |
US8188250B2 (en) * | 2008-04-28 | 2012-05-29 | Butamax(Tm) Advanced Biofuels Llc | Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans |
CA2722680A1 (en) * | 2008-05-01 | 2009-11-05 | Genomatica, Inc. | Microorganisms for the production of methacrylic acid |
US8389252B2 (en) | 2008-05-12 | 2013-03-05 | Butamax(Tm) Advanced Biofuels Llc | Yeast strain for production of four carbon alcohols |
WO2009143455A2 (en) | 2008-05-22 | 2009-11-26 | Microbia, Inc. | Engineering resistance to aliphatic alcohols |
CA2723874A1 (en) * | 2008-06-04 | 2009-12-10 | Butamaxtm Advanced Biofuels Llc | Deletion mutants for the production of isobutanol |
WO2009149260A1 (en) * | 2008-06-04 | 2009-12-10 | Solix Biofuels, Inc. | Compositions, methods and uses for growth of microorganisms and production of their products |
WO2009155382A1 (en) * | 2008-06-17 | 2009-12-23 | Genomatica, Inc. | Microorganisms and methods for the biosynthesis of fumarate, malate, and acrylate |
EP3514242A3 (en) | 2008-09-10 | 2019-08-28 | Genomatica, Inc. | Microrganisms for the production of 1,4-butanediol |
WO2010031772A2 (en) * | 2008-09-16 | 2010-03-25 | Dsm Ip Assets B.V. | Alternative butanol production process in a microbial cell |
WO2010037111A1 (en) * | 2008-09-29 | 2010-04-01 | Butamax™ Advanced Biofuels LLC | INCREASED HETEROLOGOUS Fe-S ENZYME ACTIIVTY IN YEAST |
ES2521675T3 (en) | 2008-09-29 | 2014-11-13 | Butamax Advanced Biofuels Llc | Identification and use of [2Fe-2S] -dihydroxy acid-bacterial dehydratases |
AU2009296227A1 (en) * | 2008-09-29 | 2010-04-01 | Butamax(Tm) Advanced Biofuels Llc | Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria |
US20100184173A1 (en) * | 2008-11-14 | 2010-07-22 | Genomatica, Inc. | Microorganisms for the production of methyl ethyl ketone and 2-butanol |
US8652823B2 (en) * | 2008-12-03 | 2014-02-18 | Butamax(Tm) Advanced Biofuels Llc | Strain for butanol production with increased membrane unsaturated trans fatty acids |
US20100140166A1 (en) * | 2008-12-04 | 2010-06-10 | E.I. Du Pont De Nemours And Company | Process for fermentive preparation of alcohols and recovery of product |
US20100144000A1 (en) * | 2008-12-04 | 2010-06-10 | E. I. Du Pont De Nemours And Company | Process for fermentive preparation fo alcohols and recovery of product |
US20100143995A1 (en) | 2008-12-04 | 2010-06-10 | E. I. Du Pont De Nemours And Company | Process for Fermentive Preparation of Alcohols and Recovery of Product |
US20100143999A1 (en) * | 2008-12-04 | 2010-06-10 | E.I. Du Pont De Nemours And Company | Process for fermentive preparation of alcohols and recovery of product |
US20100139154A1 (en) * | 2008-12-04 | 2010-06-10 | E.I. Du Pont De Nemours And Company | Process for fermentive preparation of alcohols and recovery of product |
US20100143992A1 (en) * | 2008-12-04 | 2010-06-10 | E. I Du Pont De Nemours And Company | Process for Fermentive Preparation of Alcohols and Recovery of Product |
US20100143994A1 (en) * | 2008-12-04 | 2010-06-10 | E. I. Du Pont De Memours And Company | Process for fermentive preparation of alcohols and recovery of product |
US20100143993A1 (en) * | 2008-12-04 | 2010-06-10 | E.I. Du Pont De Nemours And Company | Process for fermentive preparationfor alcolhols and recovery of product |
AU2009327490A1 (en) | 2008-12-16 | 2011-07-28 | Genomatica, Inc. | Microorganisms and methods for conversion of syngas and other carbon sources to useful products |
US20100159521A1 (en) * | 2008-12-19 | 2010-06-24 | E. I. Du Pont De Nemours And Company | Ozone treatment of biomass to enhance enzymatic saccharification |
RU2405826C2 (en) | 2008-12-29 | 2010-12-10 | Дэвон Инвестмент Лимитед | Method of preparing organic solvents, apparatus for realising said method, product obtained using said method |
US8557562B2 (en) * | 2008-12-29 | 2013-10-15 | Butamax(Tm) Advanced Biofuels Llc | Yeast with increased butanol tolerance involving filamentous growth response |
US8455225B2 (en) * | 2008-12-29 | 2013-06-04 | Butamax Advanced Biofuels Llc | Yeast with increased butanol tolerance involving high osmolarity/glycerol response pathway |
US8795992B2 (en) * | 2008-12-29 | 2014-08-05 | Butamax Advanced Biofuels Llc | Yeast with increased butanol tolerance involving cell wall integrity pathway |
CN102341494B (en) | 2009-01-08 | 2014-10-15 | 科德克希思公司 | Transaminase polypeptides |
RU2405828C2 (en) | 2009-01-29 | 2010-12-10 | Дэвон Инвестмент Лимитед | Method of preparing organic solvents |
US8614085B2 (en) | 2009-02-27 | 2013-12-24 | Butamax(Tm) Advanced Biofuels Llc | Yeast with increased butanol tolerance involving a multidrug efflux pump gene |
EP2449120A4 (en) | 2009-04-01 | 2013-11-20 | Xylofuel Llc | Process to produce organic compounds from synthesis gases |
CN106119113A (en) | 2009-04-30 | 2016-11-16 | 基因组股份公司 | For producing the biology of 1,3 butanediols |
WO2010127303A1 (en) | 2009-04-30 | 2010-11-04 | Genomatica, Inc. | Organisms for the production of isopropanol, n-butanol, and isobutanol |
EP2427544B1 (en) | 2009-05-07 | 2019-07-17 | Genomatica, Inc. | Microorganisms and methods for the biosynthesis of adipate, hexamethylenediamine and 6-aminocaproic acid |
JP2012526561A (en) | 2009-05-15 | 2012-11-01 | ゲノマチカ, インク. | Organisms for the production of cyclohexanone |
JP6133599B2 (en) | 2009-06-04 | 2017-05-24 | ゲノマチカ, インク. | Method for separating components of fermentation broth |
CN113528417A (en) | 2009-06-04 | 2021-10-22 | 基因组股份公司 | Microorganisms for producing 1, 4-butanediol and related methods |
WO2010144746A2 (en) * | 2009-06-10 | 2010-12-16 | Genomatica, Inc. | Microorganisms and methods for carbon-efficient biosynthesis of mek and 2-butanol |
EP3190174A1 (en) | 2009-08-05 | 2017-07-12 | Genomatica, Inc. | Semi-synthetic terephthalic acid via microorganisms that produce muconic acid |
BR112012005296A2 (en) | 2009-09-09 | 2019-01-15 | Genomatica Inc | microorganisms and methods for the co-production of isopropanol with primary alcohols, dios and acids. |
WO2011037414A2 (en) * | 2009-09-22 | 2011-03-31 | 한국과학기술원 | Recombinant mutant microorganism with increased alcohol production ability, and preparation method of alcohol using same |
BR112012006939A2 (en) * | 2009-09-29 | 2015-09-08 | Butamax Tm Advanced Biofuels | Recombinant yeast production host cell and method for producing a product selected from the group consisting of 2,3-butanediol, isobutanol, 2-butanol, 1-butanol, 2-butanone, valine, leucine, lactic acid, malic acid , alcohol, isoampyl and isoprenoids |
US20110195505A1 (en) * | 2009-10-08 | 2011-08-11 | Butamax(Tm) Advanced Biofuels Llc | Bacterial strains for butanol production |
JP2013507145A (en) | 2009-10-13 | 2013-03-04 | ゲノマチカ, インク. | Microorganisms and related methods for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA, putrescine and related compounds |
KR20120088753A (en) | 2009-10-23 | 2012-08-08 | 게노마티카 인코포레이티드 | Microorganisms for the production of aniline |
US8530210B2 (en) | 2009-11-25 | 2013-09-10 | Genomatica, Inc. | Microorganisms and methods for the coproduction 1,4-butanediol and gamma-butyrolactone |
WO2011071682A1 (en) | 2009-12-10 | 2011-06-16 | Genomatica, Inc. | Methods and organisms for converting synthesis gas or other gaseous carbon sources and methanol to 1,3-butanediol |
CN102666863A (en) | 2009-12-15 | 2012-09-12 | 思德力公司 | Method for recovery of organic components from dilute aqueous solutions |
BR112012016043A2 (en) | 2009-12-29 | 2015-11-24 | Butamax Advanced Biofuels Llc | "Recombinant yeast host cell, methods for reproducing isobutanol, for increasing glucose consumption, for enhancing the formation of a biosynthetic pathway product and for improving redox balance" |
CA2786607A1 (en) | 2010-01-08 | 2011-07-14 | Gevo, Inc. | Integrated methods of preparing renewable chemicals |
SG182686A1 (en) * | 2010-01-29 | 2012-08-30 | Genomatica Inc | Microorganisms and methods for the biosynthesis of p-toluate and terephthalate |
US8574406B2 (en) | 2010-02-09 | 2013-11-05 | Butamax Advanced Biofuels Llc | Process to remove product alcohol from a fermentation by vaporization under vacuum |
GB2501143B (en) | 2010-02-12 | 2014-03-26 | Gevo Inc | Yeast microorganisms genetically engineered to improve isobutanol biosynthesis |
WO2011103300A2 (en) | 2010-02-17 | 2011-08-25 | Butamax(Tm) Advanced Biofuels Llc | Improving activity of fe-s cluster requiring proteins |
US8048661B2 (en) | 2010-02-23 | 2011-11-01 | Genomatica, Inc. | Microbial organisms comprising exogenous nucleic acids encoding reductive TCA pathway enzymes |
US8637286B2 (en) | 2010-02-23 | 2014-01-28 | Genomatica, Inc. | Methods for increasing product yields |
US9023636B2 (en) | 2010-04-30 | 2015-05-05 | Genomatica, Inc. | Microorganisms and methods for the biosynthesis of propylene |
KR20180005263A (en) | 2010-05-05 | 2018-01-15 | 게노마티카 인코포레이티드 | Microorganisms and methods for the biosynthsis of butadiene |
EP2566830B1 (en) | 2010-05-07 | 2017-03-22 | GEVO, Inc. | Renewable jet fuel blendstock from isobutanol |
EP2582799B1 (en) | 2010-06-17 | 2017-12-20 | Codexis, Inc. | Biocatalysts and methods for the synthesis of (s)-3-(1-aminoethyl)-phenol |
AU2011268326B2 (en) | 2010-06-17 | 2015-02-05 | Butamax(Tm) Advanced Biofuels Llc | Yeast production culture for the production of butanol |
US8871488B2 (en) | 2010-06-18 | 2014-10-28 | Butamax Advanced Biofuels Llc | Recombinant host cells comprising phosphoketolases |
CA2806230A1 (en) | 2010-07-26 | 2012-02-09 | Genomatica, Inc. | Microorganisms and methods for the biosynthesis of aromatics, 2,4-pentadienoate and 1,3-butadiene |
WO2012027642A1 (en) * | 2010-08-27 | 2012-03-01 | Gevo, Inc. | Balanced four-step pathways to renewable butanols |
CA2807930A1 (en) | 2010-09-02 | 2012-03-08 | Butamax (Tm) Advanced Buofuels Llc | Process to remove product alcohol from a fermentation by vaporization under vacuum |
BR112013005386A2 (en) | 2010-09-07 | 2016-06-07 | Butamax Advanced Biofuels Llc | recombinant host cell, method, composition and use of a host cell |
DE102011003383A1 (en) * | 2011-01-31 | 2012-08-02 | Wacker Chemie Ag | Process for the fermentative production of 2,3-butanediol |
DE102011003394A1 (en) * | 2011-01-31 | 2012-08-02 | Wacker Chemie Ag | Process for the fermentative production of 2,3-butanediol |
US8759044B2 (en) | 2011-03-23 | 2014-06-24 | Butamax Advanced Biofuels Llc | In situ expression of lipase for enzymatic production of alcohol esters during fermentation |
US8765425B2 (en) | 2011-03-23 | 2014-07-01 | Butamax Advanced Biofuels Llc | In situ expression of lipase for enzymatic production of alcohol esters during fermentation |
HUE045191T2 (en) | 2011-03-24 | 2019-12-30 | Butamax Tm Advanced Biofuels | Host cells and methods for production of isobutanol |
EP2699618A4 (en) | 2011-04-19 | 2014-12-17 | Gevo Inc | Variations on prins-like chemistry to produce 2,5-dimethylhexadiene from isobutanol |
KR101298988B1 (en) * | 2011-05-18 | 2013-08-26 | 서강대학교산학협력단 | Heterologous Klebsiella pneumoniae strains for producing 2,3-butanediol and manufacturing method |
EP2721163B1 (en) | 2011-06-17 | 2016-12-21 | Butamax Advanced Biofuels LLC | Co-products from biofuel production processes and methods of making |
EP2721165A2 (en) | 2011-06-17 | 2014-04-23 | Butamax Advanced Biofuels LLC | Lignocellulosic hydrolysates as feedstocks for isobutanol fermentation |
US20130180164A1 (en) | 2011-07-28 | 2013-07-18 | Butamax(Tm) Advanced Biofuels Llc | Low sulfur fuel compositions having improved lubricity |
CA2849877A1 (en) | 2011-07-28 | 2013-01-31 | Butamax Advanced Biofuels Llc | Keto-isovalerate decarboxylase enzymes and methods of use thereof |
EP2771381B1 (en) * | 2011-10-27 | 2017-12-06 | The Regents of The University of California | Bacterial production of methyl ketones |
EP2788096A2 (en) | 2011-12-09 | 2014-10-15 | Butamax Advanced Biofuels LLC | Process to remove product alcohols from fermentation broth |
JP2015503350A (en) | 2011-12-30 | 2015-02-02 | ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー | Alcoholic fermentation production |
BR112014016005A8 (en) | 2011-12-30 | 2017-07-04 | Butamax Advanced Biofuels Llc | genetic alterations for butanol production |
US9689004B2 (en) | 2012-03-23 | 2017-06-27 | Butamax Advanced Biofuels Llc | Acetate supplemention of medium for butanologens |
CN104822820A (en) | 2012-05-04 | 2015-08-05 | 布特马斯先进生物燃料有限责任公司 | Processes and systems for alcohol production and recovery |
US9169467B2 (en) | 2012-05-11 | 2015-10-27 | Butamax Advanced Biofuels Llc | Ketol-acid reductoisomerase enzymes and methods of use |
CN111705028A (en) | 2012-06-04 | 2020-09-25 | 基因组股份公司 | Microorganisms and methods for making 4-hydroxybutyrate, 1, 4-butanediol, and related compounds |
CA2879331A1 (en) | 2012-07-17 | 2014-01-23 | Antonia Maria ROJAS MARTINEZ | Method for producing 2,3-butanediol using improved strains of raoultella planticola |
US20140024064A1 (en) | 2012-07-23 | 2014-01-23 | Butamax(Tm) Advanced Biofuels Llc | Processes and systems for the production of fermentative alcohols |
CA2881407A1 (en) | 2012-08-22 | 2014-02-27 | Butamax Advanced Biofuels Llc | Production of fermentation products |
JP6302471B2 (en) * | 2012-08-28 | 2018-03-28 | ランザテク・ニュージーランド・リミテッド | Recombinant microorganisms and uses thereof |
EP2895612A1 (en) | 2012-09-12 | 2015-07-22 | Butamax Advanced Biofuels LLC | Processes and systems for the production of fermentation products |
US9840724B2 (en) | 2012-09-21 | 2017-12-12 | Butamax Advanced Biofuels Llc | Production of renewable hydrocarbon compositions |
WO2014052670A1 (en) | 2012-09-26 | 2014-04-03 | Butamax (Tm) Advanced Biofuels Llc | Polypeptides with ketol-acid reductoisomerase activity |
US20140093931A1 (en) | 2012-09-28 | 2014-04-03 | Butamax Advanced Biofuels Llc | Production of fermentation products |
WO2014052920A2 (en) * | 2012-09-28 | 2014-04-03 | The Regents Of The University Of California | Methods of producing acetoin and 2,3-butanediol using photosynthetic microorganisms |
US9273330B2 (en) | 2012-10-03 | 2016-03-01 | Butamax Advanced Biofuels Llc | Butanol tolerance in microorganisms |
EP2906706B1 (en) | 2012-10-11 | 2020-02-26 | Butamax Advanced Biofuels LLC | Processes and systems for the production of fermentation products |
WO2014074701A1 (en) | 2012-11-07 | 2014-05-15 | Ohio University | Ionization of chemicals in mixture at low ph by ambient ionization / mass spectrometry |
WO2014074699A1 (en) * | 2012-11-07 | 2014-05-15 | Ohio University | Online monitoring of fuel cell reactions by desorption electrospray mass spectrometry |
US10288610B2 (en) * | 2012-11-13 | 2019-05-14 | The General Hospital Corporation | Vitro assays for detecting Salmonella enterica serotype typhi |
WO2014092562A1 (en) * | 2012-12-11 | 2014-06-19 | Photanol B.V. | Synthesis of acetoin, 2,3-butanediol and 2-butanol by cyanobacteria by heterologous expression of a catabolic pathway |
US20140178954A1 (en) | 2012-12-20 | 2014-06-26 | E I Du Pont De Nemours And Company | Expression of xylose isomerase activity in yeast |
WO2014106107A2 (en) | 2012-12-28 | 2014-07-03 | Butamax (Tm) Advanced Biofuels Llc | Dhad variants for butanol production |
WO2014105840A1 (en) | 2012-12-31 | 2014-07-03 | Butamax Advanced Biofuels Llc | Fermentative production of alcohols |
US8669076B1 (en) | 2013-03-11 | 2014-03-11 | E I Du Pont De Nemours And Company | Cow rumen xylose isomerases active in yeast cells |
US9187743B2 (en) | 2013-03-11 | 2015-11-17 | E I Du Pont De Nemours And Company | Bacterial xylose isomerases active in yeast cells |
US20140273105A1 (en) | 2013-03-12 | 2014-09-18 | E I Du Pont De Nemours And Company | Gradient pretreatment of lignocellulosic biomass |
WO2014159309A1 (en) | 2013-03-12 | 2014-10-02 | Butamax Advanced Biofuels Llc | Processes and systems for the production of alcohols |
WO2014151190A1 (en) | 2013-03-15 | 2014-09-25 | Butamax Advanced Biofuels Llc | Dhad variants and methods of screening |
US10227623B2 (en) | 2013-11-24 | 2019-03-12 | E I Du Pont De Nemours And Company | High force and high stress destructuring of cellulosic biomass |
BR112016012634A2 (en) * | 2013-12-03 | 2017-09-26 | Lanzatech New Zealand Ltd | recombinant acetogenic carboxidotrophic bacteria, and methods for producing acetone and mek |
CN103627698A (en) * | 2013-12-05 | 2014-03-12 | 江南大学 | Breeding of acetoin high-tolerance bacterial strain and acetoin fermentation production with bacterial strain |
CN105219666B (en) * | 2014-06-18 | 2021-03-26 | 清华大学 | Symbiotic bacteria system and method for producing butanol under micro-aerobic conditions |
CA2957920A1 (en) | 2014-08-11 | 2016-02-18 | Butamax Advanced Biofuels Llc | Yeast preparations and methods of making the same |
ES2836800T3 (en) * | 2014-12-08 | 2021-06-28 | Lanzatech New Zealand Ltd | Recombinant microorganisms exhibiting increased flux through a fermentation pathway |
US10793879B2 (en) * | 2014-12-31 | 2020-10-06 | Poet Research, Inc. | High purity starch stream methods and systems |
DK3387111T3 (en) * | 2015-12-11 | 2021-05-10 | Wacker Chemie Ag | MICRO-ORGANISM STEM AND PROCEDURE FOR ANTIBIOTIC-FREE, FERMENTATIVE MANUFACTURE OF LOW MOLECULAR SUBSTANCES AND PROTEINS |
US20190112590A1 (en) | 2016-04-08 | 2019-04-18 | Ei Du Pont De Nemours And Company | Arabinose isomerases for yeast |
KR102028161B1 (en) * | 2017-01-10 | 2019-10-02 | 경희대학교 산학협력단 | Process for preparing 2,3-butanediol using transformant |
WO2018131898A2 (en) | 2017-01-10 | 2018-07-19 | 경희대학교 산학협력단 | Novel use of methylomonas sp. dh-1 strain |
BR112019015754A2 (en) * | 2017-01-30 | 2020-06-09 | Intrexon Corp | methods and microorganisms for making 2,3-butanediol and derivatives thereof from c1 carbons |
EP3615676A4 (en) | 2017-04-28 | 2021-07-07 | Intrexon Corporation | Methods and microorganisms for the fermentation of methane to multi-carbon compounds |
US20200291366A1 (en) * | 2017-09-12 | 2020-09-17 | Igc Bio, Inc. | Pross optimized enzymes |
US11788071B2 (en) | 2020-04-10 | 2023-10-17 | Codexis, Inc. | Engineered transaminase polypeptides |
FR3115988A1 (en) | 2020-11-06 | 2022-05-13 | Global Bioenergies | Make-up composition based on biosourced isododecane and process for its preparation |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6432688B1 (en) | 1999-01-18 | 2002-08-13 | Daicel Chemical Industries, Ltd. | Amino alcohol dehydrogenase converts keto alcohol to amino alcohol and amino alcohol to keto alcohol |
US6514733B1 (en) | 1999-08-18 | 2003-02-04 | E. I. Du Pont De Nemours And Company | Process for the biological production of 1,3-propanediol with high titer |
US6586229B1 (en) | 2000-06-01 | 2003-07-01 | North Carolina State University | Method for the production of ρ-Hydroxybenzoate in species of pseudomonas and agrobacterium |
WO2003089621A2 (en) | 2002-04-22 | 2003-10-30 | E. I. Du Pont De Nemours And Company | Promoter and plasmid system for genetic engineering |
US20070031918A1 (en) | 2005-04-12 | 2007-02-08 | Dunson James B Jr | Treatment of biomass to obtain fermentable sugars |
Family Cites Families (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2198104A (en) * | 1938-12-15 | 1940-04-23 | Commercial Solvents Corp | Butyl alcohol fermentation process |
US2730542A (en) | 1951-06-25 | 1956-01-10 | Ferrari Vittorio Emiliano | Process for preparing ethanol-2-aminophosphoric acid |
US4424275A (en) | 1979-09-17 | 1984-01-03 | Sidney Levy | Continuous process for producing N-butanol employing anaerobic fermentation |
US4568643A (en) | 1981-08-03 | 1986-02-04 | Sidney Levy | Continuous process for producing n-butanol employing anaerobic fermentation |
US4520104A (en) | 1982-11-18 | 1985-05-28 | Cpc International Inc. | Production of butanol by a continuous fermentation process |
JPS61209594A (en) | 1985-03-14 | 1986-09-17 | Res Assoc Petroleum Alternat Dev<Rapad> | Production of alcohol |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
JPS6317695A (en) | 1986-07-09 | 1988-01-25 | Idemitsu Kosan Co Ltd | Production of alcohol |
JPS63102687A (en) | 1986-10-20 | 1988-05-07 | Res Assoc Petroleum Alternat Dev<Rapad> | Production of butanol |
AT388174B (en) | 1987-03-10 | 1989-05-10 | Vogelbusch Gmbh | METHOD FOR THE FERMENTATION OF CARBOHYDRATE-CONTAINING MEDIA USING BACTERIA |
JPS63254986A (en) | 1987-04-10 | 1988-10-21 | Res Assoc Petroleum Alternat Dev<Rapad> | Production of alcohol |
JPH084515B2 (en) | 1987-11-09 | 1996-01-24 | 出光興産株式会社 | Method for producing organic compound |
US5424202A (en) * | 1988-08-31 | 1995-06-13 | The University Of Florida | Ethanol production by recombinant hosts |
US5000000A (en) | 1988-08-31 | 1991-03-19 | University Of Florida | Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes |
US5063156A (en) * | 1990-04-30 | 1991-11-05 | Glassner David A | Process for the fermentative production of acetone, butanol and ethanol |
US5192673A (en) * | 1990-04-30 | 1993-03-09 | Michigan Biotechnology Institute | Mutant strain of C. acetobutylicum and process for making butanol |
WO1993002463A1 (en) | 1991-07-24 | 1993-02-04 | Elin Energieversorgung Gesellschaft M.B.H. | Pole for a power switch |
US5210032A (en) | 1992-01-21 | 1993-05-11 | Trustees Of The Boston University | Degeneration-resistant solventogenic clostridia |
US5523223A (en) * | 1992-03-13 | 1996-06-04 | Forschungszentrum Julich Gmbh | Ketoester reductase for conversion of keto acid esters to optically active hydroxy acid esters |
JP3574682B2 (en) | 1993-09-24 | 2004-10-06 | ダイセル化学工業株式会社 | Novel enzymes, methods for producing the enzymes, DNAs encoding the enzymes, transformants containing the DNAs, methods for producing optically active alcohols and the like using the enzymes |
US5686276A (en) * | 1995-05-12 | 1997-11-11 | E. I. Du Pont De Nemours And Company | Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism |
US6358717B1 (en) | 1997-05-14 | 2002-03-19 | The Board Of Trustees Of The University Of Illinois | Method of producing butanol using a mutant strain of Clostridium beijerinckii |
US7074608B1 (en) | 1998-05-12 | 2006-07-11 | E. I. Du Pont De Nemours And Company | Method for the production of 1,3-propanediol by recombinant organisms comprising genes for coenzyme B12 synthesis |
EP1149918A1 (en) | 2000-04-27 | 2001-10-31 | Creavis Gesellschaft für Technologie und Innovation mbH | Process for the oxidation of hydrocarbons by use of microorganisms |
US6960465B1 (en) * | 2001-06-27 | 2005-11-01 | Northwestern University | Increased cell resistance to toxic organic substances |
CA2474905A1 (en) * | 2002-03-18 | 2003-09-25 | Ciba Specialty Chemicals Holding Inc. | Alcohol dehydrogenases with high solvent and temperature stability |
BRPI0314498B1 (en) | 2002-10-04 | 2019-08-20 | E.I. Du Pont De Nemours And Company | Transgenic Escherichia coli and the method for the production of 1,3-propanediol |
MXPA05009553A (en) * | 2003-03-10 | 2006-03-17 | Broin And Associates Inc | Method for producing ethanol using raw starch. |
JP4294382B2 (en) * | 2003-06-06 | 2009-07-08 | ダイセル化学工業株式会社 | (2S, 3S) -2,3-butanediol dehydrogenase |
AT413541B (en) | 2004-05-10 | 2006-03-15 | Iep Gmbh | PROCESS FOR THE PREPARATION OF 2-BUTANOL |
US8962298B2 (en) * | 2006-05-02 | 2015-02-24 | Butamax Advanced Biofuels Llc | Recombinant host cell comprising a diol dehydratase |
US8828704B2 (en) * | 2006-05-02 | 2014-09-09 | Butamax Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
US7659104B2 (en) * | 2006-05-05 | 2010-02-09 | E.I. Du Pont De Nemours And Company | Solvent tolerant microorganisms and methods of isolation |
US7541173B2 (en) * | 2006-06-15 | 2009-06-02 | E.I. Du Pont De Nemours And Company | Solvent tolerant microorganisms and methods of isolation |
WO2008006038A2 (en) | 2006-07-06 | 2008-01-10 | Integrated Genomics, Inc. | Methods and compositions for butanol production |
US8426174B2 (en) * | 2007-05-02 | 2013-04-23 | Butamax(Tm) Advanced Biofuels Llc | Method for the production of 2-butanol |
US8188250B2 (en) * | 2008-04-28 | 2012-05-29 | Butamax(Tm) Advanced Biofuels Llc | Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans |
-
2007
- 2007-04-30 US US11/741,916 patent/US8962298B2/en active Active
- 2007-04-30 US US11/741,892 patent/US8206970B2/en active Active
- 2007-05-02 JP JP2009509715A patent/JP2009535062A/en active Pending
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- 2007-05-02 CA CA002645497A patent/CA2645497A1/en not_active Abandoned
- 2007-05-02 BR BRPI0710414-6A patent/BRPI0710414A2/en not_active Application Discontinuation
- 2007-05-02 MX MX2008013882A patent/MX2008013882A/en active IP Right Grant
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- 2007-05-02 MX MX2008013881A patent/MX2008013881A/en active IP Right Grant
- 2007-05-02 CA CA002646641A patent/CA2646641A1/en not_active Abandoned
- 2007-05-02 WO PCT/US2007/010744 patent/WO2007130521A2/en active Application Filing
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-
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-
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- 2011-10-26 US US13/281,872 patent/US20120196341A1/en not_active Abandoned
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6432688B1 (en) | 1999-01-18 | 2002-08-13 | Daicel Chemical Industries, Ltd. | Amino alcohol dehydrogenase converts keto alcohol to amino alcohol and amino alcohol to keto alcohol |
US6514733B1 (en) | 1999-08-18 | 2003-02-04 | E. I. Du Pont De Nemours And Company | Process for the biological production of 1,3-propanediol with high titer |
US6586229B1 (en) | 2000-06-01 | 2003-07-01 | North Carolina State University | Method for the production of ρ-Hydroxybenzoate in species of pseudomonas and agrobacterium |
WO2003089621A2 (en) | 2002-04-22 | 2003-10-30 | E. I. Du Pont De Nemours And Company | Promoter and plasmid system for genetic engineering |
US20070031918A1 (en) | 2005-04-12 | 2007-02-08 | Dunson James B Jr | Treatment of biomass to obtain fermentable sugars |
Non-Patent Citations (10)
Title |
---|
BACHOVCHIN ET AL., BIOCHEMISTRY, vol. 16, 1977, pages 1082 - 1092 |
DESHPANDE, MUKUND V., APPL. BIOCHEM. BIOTECHNOL., vol. 36, 1992, pages 227 |
DURBIN ET AL.: "Biological sequence analysis: probabilistic models of proteins and nucleic acids", 1998, CAMBRIDGE UNIVERSITY PRESS |
FLEMING ET AL., APPL. ENVIRON. MICROBIOL., vol. 61, no. 11, 1995, pages 3775 - 3780 |
JONES ET AL., BIOCHEM. J., vol. 134, 1973, pages 167 - 182 |
KROGH ET AL., J. MOL. BIOL., vol. 235, 1994, pages 1501 - 1531 |
SAMBROOK, J.; FRITSCH, E. F.; MANIATIS, T.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY |
SULTER ET AL., ARCH. MICROBIOL., vol. 153, 1990, pages 485 - 489 |
TAGHAVI ET AL., APPL. ENVIRON. MICROBIOL., vol. 60, no. 10, 1994, pages 3585 - 3591 |
THOMAS D. BROCK: "Biotechnology: A Textbook of Industrial Microbiology", 1989, SINAUER ASSOCIATES, INC. |
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