WO2008024319A3 - Microfluidic devices for nucleic acid assembly - Google Patents
Microfluidic devices for nucleic acid assembly Download PDFInfo
- Publication number
- WO2008024319A3 WO2008024319A3 PCT/US2007/018437 US2007018437W WO2008024319A3 WO 2008024319 A3 WO2008024319 A3 WO 2008024319A3 US 2007018437 W US2007018437 W US 2007018437W WO 2008024319 A3 WO2008024319 A3 WO 2008024319A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- microfluidic devices
- reagents
- acid assembly
- devices
- Prior art date
Links
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00279—Features relating to reactor vessels
- B01J2219/00281—Individual reactor vessels
- B01J2219/00286—Reactor vessels with top and bottom openings
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00279—Features relating to reactor vessels
- B01J2219/00306—Reactor vessels in a multiple arrangement
- B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
- B01J2219/00315—Microtiter plates
- B01J2219/00317—Microwell devices, i.e. having large numbers of wells
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00423—Means for dispensing and evacuation of reagents using filtration, e.g. through porous frits
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00585—Parallel processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/0059—Sequential processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00781—Aspects relating to microreactors
- B01J2219/00905—Separation
- B01J2219/00912—Separation by electrophoresis
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
- B01L2300/0838—Capillaries
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
Abstract
Certain aspects of the present invention provide devices and methods for assembling nucleic acid molecules on micro fluidic devices, e.g. via extension and/or ligation reactions. The invention also provides systems comprising one or more acoustic liquid handlers, one or more microfluidic devices, and/or one or more robotic liquid handlers for automating a nucleic acid assembly procedure. The microdevice may comprise the following components: a sipper, or means for transferring reagents (310); a main channel (315); a spitter, or means for transferring reagents (320), sample wells for storage of reagents or buffers (330, 335, 340), separation or concentration channels (350, 355) and sample or reaction wells (360, 365) e.g. a thermocycler.
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US83896006P | 2006-08-20 | 2006-08-20 | |
US60/838,960 | 2006-08-20 | ||
US87833206P | 2006-12-31 | 2006-12-31 | |
US87833006P | 2006-12-31 | 2006-12-31 | |
US60/878,330 | 2006-12-31 | ||
US60/878,332 | 2006-12-31 |
Publications (3)
Publication Number | Publication Date |
---|---|
WO2008024319A2 WO2008024319A2 (en) | 2008-02-28 |
WO2008024319A3 true WO2008024319A3 (en) | 2008-04-10 |
WO2008024319A8 WO2008024319A8 (en) | 2008-05-29 |
Family
ID=38996695
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2007/018437 WO2008024319A2 (en) | 2006-08-20 | 2007-08-20 | Microfluidic devices for nucleic acid assembly |
Country Status (1)
Country | Link |
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WO (1) | WO2008024319A2 (en) |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8388908B2 (en) | 2009-06-02 | 2013-03-05 | Integenx Inc. | Fluidic devices with diaphragm valves |
US8394642B2 (en) | 2009-06-05 | 2013-03-12 | Integenx Inc. | Universal sample preparation system and use in an integrated analysis system |
US8431340B2 (en) | 2004-09-15 | 2013-04-30 | Integenx Inc. | Methods for processing and analyzing nucleic acid samples |
US8476063B2 (en) | 2004-09-15 | 2013-07-02 | Integenx Inc. | Microfluidic devices |
US8512538B2 (en) | 2010-05-28 | 2013-08-20 | Integenx Inc. | Capillary electrophoresis device |
US8557518B2 (en) | 2007-02-05 | 2013-10-15 | Integenx Inc. | Microfluidic and nanofluidic devices, systems, and applications |
US8584703B2 (en) | 2009-12-01 | 2013-11-19 | Integenx Inc. | Device with diaphragm valve |
US8672532B2 (en) | 2008-12-31 | 2014-03-18 | Integenx Inc. | Microfluidic methods |
US8748165B2 (en) | 2008-01-22 | 2014-06-10 | Integenx Inc. | Methods for generating short tandem repeat (STR) profiles |
US8763642B2 (en) | 2010-08-20 | 2014-07-01 | Integenx Inc. | Microfluidic devices with mechanically-sealed diaphragm valves |
US9121058B2 (en) | 2010-08-20 | 2015-09-01 | Integenx Inc. | Linear valve arrays |
Families Citing this family (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7563600B2 (en) | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
US8053191B2 (en) | 2006-08-31 | 2011-11-08 | Westend Asset Clearinghouse Company, Llc | Iterative nucleic acid assembly using activation of vector-encoded traits |
GB0815472D0 (en) * | 2008-08-22 | 2008-10-01 | Iti Scotland Ltd | Microfluidic system |
US8808986B2 (en) | 2008-08-27 | 2014-08-19 | Gen9, Inc. | Methods and devices for high fidelity polynucleotide synthesis |
WO2011056872A2 (en) | 2009-11-03 | 2011-05-12 | Gen9, Inc. | Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly |
EP3085791A1 (en) | 2009-11-25 | 2016-10-26 | Gen9, Inc. | Methods and apparatuses for chip-based dna error reduction |
WO2011066185A1 (en) | 2009-11-25 | 2011-06-03 | Gen9, Inc. | Microfluidic devices and methods for gene synthesis |
US9217144B2 (en) | 2010-01-07 | 2015-12-22 | Gen9, Inc. | Assembly of high fidelity polynucleotides |
US8716467B2 (en) | 2010-03-03 | 2014-05-06 | Gen9, Inc. | Methods and devices for nucleic acid synthesis |
GB2481425A (en) * | 2010-06-23 | 2011-12-28 | Iti Scotland Ltd | Method and device for assembling polynucleic acid sequences |
US10457935B2 (en) | 2010-11-12 | 2019-10-29 | Gen9, Inc. | Protein arrays and methods of using and making the same |
US9295965B2 (en) | 2010-11-12 | 2016-03-29 | Gen9, Inc. | Methods and devices for nucleic acid synthesis |
US10131903B2 (en) * | 2011-04-01 | 2018-11-20 | The Regents Of The University Of California | Microfluidic platform for synthetic biology applications |
US9752176B2 (en) | 2011-06-15 | 2017-09-05 | Ginkgo Bioworks, Inc. | Methods for preparative in vitro cloning |
AU2012300401B2 (en) | 2011-08-26 | 2018-02-08 | Gen9, Inc. | Compositions and methods for high fidelity assembly of nucleic acids |
US20150136604A1 (en) | 2011-10-21 | 2015-05-21 | Integenx Inc. | Sample preparation, processing and analysis systems |
US10865440B2 (en) | 2011-10-21 | 2020-12-15 | IntegenX, Inc. | Sample preparation, processing and analysis systems |
US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
CN111876409A (en) | 2012-04-24 | 2020-11-03 | Gen9股份有限公司 | Method for sorting nucleic acids and multiplex preparations in vitro cloning |
CN104685116A (en) | 2012-06-25 | 2015-06-03 | Gen9股份有限公司 | Methods for nucleic acid assembly and high throughput sequencing |
CA2880687C (en) * | 2012-08-16 | 2023-09-19 | Synthetic Genomics, Inc. | Digital to biological converter |
EP3071333A4 (en) | 2013-11-18 | 2017-11-15 | IntegenX Inc. | Cartridges and instruments for sample analysis |
WO2015179098A1 (en) | 2014-05-21 | 2015-11-26 | Integenx Inc. | Fluidic cartridge with valve mechanism |
EP3552690A1 (en) | 2014-10-22 | 2019-10-16 | IntegenX Inc. | Systems and methods for sample preparation, processing and analysis |
EP3366370A1 (en) * | 2017-02-22 | 2018-08-29 | Briefcase Biotec GmbH | Device for the synthesis of oligonucleotides |
WO2019074512A1 (en) * | 2017-10-12 | 2019-04-18 | Hewlett-Packard Development Company, L.P. | Embedded microfluidic devices |
US11249100B2 (en) | 2018-01-05 | 2022-02-15 | Worcester Polytechnic Institute | Modular robotic systems for delivering fluid to microfluidic devices |
US10046322B1 (en) | 2018-03-22 | 2018-08-14 | Talis Biomedical Corporation | Reaction well for assay device |
WO2019209273A1 (en) | 2018-04-24 | 2019-10-31 | Hewlett-Packard Development Company, L.P. | Microfluidic devices |
WO2019209375A1 (en) | 2018-04-24 | 2019-10-31 | Hewlett-Packard Development Company, L.P. | Droplet ejectors to draw fluids through microfluidic networks |
US11325380B2 (en) | 2018-07-17 | 2022-05-10 | Hewlett-Packard Development Company, L.P. | Droplet ejectors to provide fluids to droplet ejectors |
US11547993B2 (en) | 2018-07-17 | 2023-01-10 | Hewlett-Packard Development Company, L.P. | Droplet ejectors with target media |
WO2020264260A1 (en) * | 2019-06-27 | 2020-12-30 | Zymergen Inc. | Laboratory automation system implementing efficient path for material and labware transfers |
US11008627B2 (en) | 2019-08-15 | 2021-05-18 | Talis Biomedical Corporation | Diagnostic system |
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Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8551714B2 (en) | 2004-09-15 | 2013-10-08 | Integenx Inc. | Microfluidic devices |
US8431340B2 (en) | 2004-09-15 | 2013-04-30 | Integenx Inc. | Methods for processing and analyzing nucleic acid samples |
US8431390B2 (en) | 2004-09-15 | 2013-04-30 | Integenx Inc. | Systems of sample processing having a macro-micro interface |
US8476063B2 (en) | 2004-09-15 | 2013-07-02 | Integenx Inc. | Microfluidic devices |
US8557518B2 (en) | 2007-02-05 | 2013-10-15 | Integenx Inc. | Microfluidic and nanofluidic devices, systems, and applications |
US8748165B2 (en) | 2008-01-22 | 2014-06-10 | Integenx Inc. | Methods for generating short tandem repeat (STR) profiles |
US8672532B2 (en) | 2008-12-31 | 2014-03-18 | Integenx Inc. | Microfluidic methods |
US8388908B2 (en) | 2009-06-02 | 2013-03-05 | Integenx Inc. | Fluidic devices with diaphragm valves |
US8562918B2 (en) | 2009-06-05 | 2013-10-22 | Integenx Inc. | Universal sample preparation system and use in an integrated analysis system |
US8394642B2 (en) | 2009-06-05 | 2013-03-12 | Integenx Inc. | Universal sample preparation system and use in an integrated analysis system |
US9012236B2 (en) | 2009-06-05 | 2015-04-21 | Integenx Inc. | Universal sample preparation system and use in an integrated analysis system |
US8584703B2 (en) | 2009-12-01 | 2013-11-19 | Integenx Inc. | Device with diaphragm valve |
US8512538B2 (en) | 2010-05-28 | 2013-08-20 | Integenx Inc. | Capillary electrophoresis device |
US8763642B2 (en) | 2010-08-20 | 2014-07-01 | Integenx Inc. | Microfluidic devices with mechanically-sealed diaphragm valves |
US9121058B2 (en) | 2010-08-20 | 2015-09-01 | Integenx Inc. | Linear valve arrays |
Also Published As
Publication number | Publication date |
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WO2008024319A2 (en) | 2008-02-28 |
WO2008024319A8 (en) | 2008-05-29 |
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