WO2008031020A3 - Arginine wash in protein purification using affinity chromatography - Google Patents

Arginine wash in protein purification using affinity chromatography Download PDF

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Publication number
WO2008031020A3
WO2008031020A3 PCT/US2007/077865 US2007077865W WO2008031020A3 WO 2008031020 A3 WO2008031020 A3 WO 2008031020A3 US 2007077865 W US2007077865 W US 2007077865W WO 2008031020 A3 WO2008031020 A3 WO 2008031020A3
Authority
WO
WIPO (PCT)
Prior art keywords
affinity chromatography
protein purification
product
arginine
arginine wash
Prior art date
Application number
PCT/US2007/077865
Other languages
French (fr)
Other versions
WO2008031020A2 (en
Inventor
Shujun Sun
Christopher Gallo
Original Assignee
Wyeth Corp
Shujun Sun
Christopher Gallo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
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Application filed by Wyeth Corp, Shujun Sun, Christopher Gallo filed Critical Wyeth Corp
Priority to EP07814753.5A priority Critical patent/EP2059258B1/en
Priority to CA2661645A priority patent/CA2661645C/en
Priority to MX2009002576A priority patent/MX2009002576A/en
Priority to JP2009527581A priority patent/JP5386354B2/en
Priority to BRPI0716597-8A2A priority patent/BRPI0716597A2/en
Priority to AU2007292253A priority patent/AU2007292253A1/en
Priority to ES07814753T priority patent/ES2764222T3/en
Publication of WO2008031020A2 publication Critical patent/WO2008031020A2/en
Publication of WO2008031020A3 publication Critical patent/WO2008031020A3/en
Priority to IL197053A priority patent/IL197053A0/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

The invention relates to methods for isolating a product and/or reducing turbidity and/or impurities from a load fluid comprising the product and one or more impurities by passing the load fluid through a medium, followed by at least one wash solution comprising arginine or an arginine derivative, and collecting the product using an elution solution. The invention further relates to a product prepared using a method as described herein.
PCT/US2007/077865 2006-09-08 2007-09-07 Arginine wash in protein purification using affinity chromatography WO2008031020A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
EP07814753.5A EP2059258B1 (en) 2006-09-08 2007-09-07 Arginine wash in protein purification using affinity chromatography
CA2661645A CA2661645C (en) 2006-09-08 2007-09-07 Arginine wash in protein purification using affinity chromatography
MX2009002576A MX2009002576A (en) 2006-09-08 2007-09-07 Arginine wash in protein purification using affinity chromatography.
JP2009527581A JP5386354B2 (en) 2006-09-08 2007-09-07 Arginine washing in protein purification using affinity chromatography
BRPI0716597-8A2A BRPI0716597A2 (en) 2006-09-08 2007-09-07 Method for the isolation of a product; PRODUCT PREPARED BY THE METHOD; METHOD FOR INSULATING ANTIBODY; METHOD FOR REDUCING THE TURVATION OF A PRODUCT UNDERSTANDING ELUTE; AND METHOD FOR REDUCING TURVATION AND IMPURSES OF AN ELDER UNDERSTANDING A PRODUCT
AU2007292253A AU2007292253A1 (en) 2006-09-08 2007-09-07 Arginine wash in protein purification using affinity chromatography
ES07814753T ES2764222T3 (en) 2006-09-08 2007-09-07 Arginine wash in protein purification using affinity chromatography
IL197053A IL197053A0 (en) 2006-09-08 2009-02-15 Arginine wash in protein purification using affinity chromatography

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US84308406P 2006-09-08 2006-09-08
US60/843,084 2006-09-08

Publications (2)

Publication Number Publication Date
WO2008031020A2 WO2008031020A2 (en) 2008-03-13
WO2008031020A3 true WO2008031020A3 (en) 2008-10-23

Family

ID=39158086

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/077865 WO2008031020A2 (en) 2006-09-08 2007-09-07 Arginine wash in protein purification using affinity chromatography

Country Status (17)

Country Link
US (2) US8350013B2 (en)
EP (1) EP2059258B1 (en)
JP (1) JP5386354B2 (en)
KR (1) KR20090064377A (en)
CN (1) CN101511387A (en)
AR (1) AR062711A1 (en)
AU (1) AU2007292253A1 (en)
BR (1) BRPI0716597A2 (en)
CA (1) CA2661645C (en)
CL (1) CL2007002615A1 (en)
ES (1) ES2764222T3 (en)
IL (1) IL197053A0 (en)
MX (1) MX2009002576A (en)
PE (1) PE20080913A1 (en)
RU (1) RU2009106083A (en)
TW (1) TW200821026A (en)
WO (1) WO2008031020A2 (en)

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