WO2008120218A3 - Undifferentiated stem cell culture systems - Google Patents

Undifferentiated stem cell culture systems Download PDF

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Publication number
WO2008120218A3
WO2008120218A3 PCT/IL2008/000460 IL2008000460W WO2008120218A3 WO 2008120218 A3 WO2008120218 A3 WO 2008120218A3 IL 2008000460 W IL2008000460 W IL 2008000460W WO 2008120218 A3 WO2008120218 A3 WO 2008120218A3
Authority
WO
WIPO (PCT)
Prior art keywords
undifferentiated pluripotent
medium
methods
basic
serum replacement
Prior art date
Application number
PCT/IL2008/000460
Other languages
French (fr)
Other versions
WO2008120218A2 (en
Inventor
Benjamin Reubinoff
Debora Steiner
Original Assignee
Hadasit Medical Research Services & Development Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hadasit Medical Research Services & Development Limited filed Critical Hadasit Medical Research Services & Development Limited
Priority to EP08738164A priority Critical patent/EP2142641A4/en
Publication of WO2008120218A2 publication Critical patent/WO2008120218A2/en
Priority to IL201166A priority patent/IL201166A/en
Priority to US12/570,457 priority patent/US20100093091A1/en
Publication of WO2008120218A3 publication Critical patent/WO2008120218A3/en
Priority to US13/357,948 priority patent/US8940537B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Abstract

The present disclosure provides methods for maintaining and propagating undifferentiated pluripotent stem cells (SC) in suspension. The methods comprise culturing such SC in a non-adherent culture dish under conditions comprising a basic serum free medium and one or more of a basic medium, a serum replacement, an extra cellular matrix component and a factor supporting expansion of said SC. A specific and preferred culture condition comprise supplementing Neurobasal™ medium with KO serum replacement (KOSR). These conditions allowed for large scale and long term propagation of undifferentiated pluripotent SC. The culture system comprising suspended undifferentiated pluripotent SC were found to have many applications including in methods for directed as well as spontaneous differentiation of the SC into somatic cells. Also disclosed herein is a method of deriving SC, preferably human embryonic SC from human embryos via the formation of cell clusters.
PCT/IL2008/000460 2007-04-02 2008-04-02 Undifferentiated stem cell culture systems WO2008120218A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP08738164A EP2142641A4 (en) 2007-04-02 2008-04-02 Undifferentiated stem cell culture systems
IL201166A IL201166A (en) 2007-04-02 2009-09-24 Methods of expanding undifferentiated pluripotent stem cells in an undifferentiated state, culture systems comprising suspensions of stem cells in undifferentiated state and uses of the culture systems
US12/570,457 US20100093091A1 (en) 2007-04-02 2009-09-30 Undifferentiated Stem Cell Culture Systems
US13/357,948 US8940537B2 (en) 2007-04-02 2012-01-25 Undifferentiated stem cell culture systems

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/730,560 US8597947B2 (en) 2004-12-29 2007-04-02 Undifferentiated stem cell culture systems
US11/730,560 2007-04-02

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US11/730,560 Continuation-In-Part US8597947B2 (en) 2004-12-29 2007-04-02 Undifferentiated stem cell culture systems

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/570,457 Continuation US20100093091A1 (en) 2007-04-02 2009-09-30 Undifferentiated Stem Cell Culture Systems

Publications (2)

Publication Number Publication Date
WO2008120218A2 WO2008120218A2 (en) 2008-10-09
WO2008120218A3 true WO2008120218A3 (en) 2010-02-25

Family

ID=39870128

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2008/000460 WO2008120218A2 (en) 2007-04-02 2008-04-02 Undifferentiated stem cell culture systems

Country Status (3)

Country Link
US (3) US8597947B2 (en)
EP (1) EP2142641A4 (en)
WO (1) WO2008120218A2 (en)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2571355T3 (en) 2002-12-16 2016-05-24 Technion Res & Dev Foundation Culture system without feeder cells or xenocontaminants for human embryonic stem cells
EP1962719A4 (en) 2005-08-29 2011-05-04 Technion Res And Dev Of Foundation Ltd Media for culturing stem cells
EP3441459B1 (en) 2006-08-02 2021-03-17 Technion Research & Development Foundation Limited Methods of expanding embryonic stem cells in a suspension culture
US8623650B2 (en) 2007-10-19 2014-01-07 Viacyte, Inc. Methods and compositions for feeder-free pluripotent stem cell media containing human serum
WO2009124213A2 (en) * 2008-04-02 2009-10-08 The Trustees Of Columbia University In The City Of New York Dental stem cell differentiation
US9752124B2 (en) 2009-02-03 2017-09-05 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
EP2412800A1 (en) 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
PL2393917T3 (en) 2009-02-03 2016-12-30 Culture medium for epithelial stem cells and organoids comprising said stem cells.
CA2783437C (en) 2009-11-12 2020-08-04 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
WO2012009377A2 (en) 2010-07-12 2012-01-19 University Of Southern California Biocompatible substrate for facilitating interconnections between stem cells and target tissues and methods for implanting same
CN103354835B (en) * 2010-12-08 2016-03-02 韦尔赛特公司 Suppress reagent and the method for human pluripotent stem cells growth
JP6148429B2 (en) * 2011-01-31 2017-06-14 協和発酵バイオ株式会社 Method for culturing human pluripotent stem cells
WO2012105505A1 (en) * 2011-01-31 2012-08-09 独立行政法人国立国際医療研究センター Highly functional liver cells derived from pluripotent stem cells, method for producing same, and method for testing metabolism/toxicity of drug
DK2694644T3 (en) 2011-03-30 2018-04-16 Cellular Dynamics Int Inc Priming of pluripotent stem cells for neural differentiation
US10478206B2 (en) 2011-04-29 2019-11-19 University Of Southern California Instruments and methods for the implantation of cell-seeded substrates
US8877489B2 (en) 2011-12-05 2014-11-04 California Institute Of Technology Ultrathin parylene-C semipermeable membranes for biomedical applications
WO2013077423A1 (en) 2011-11-25 2013-05-30 国立大学法人京都大学 Method for culturing pluripotent stem cell
US9248013B2 (en) 2011-12-05 2016-02-02 California Institute Of Technology 3-Dimensional parylene scaffold cage
US20150072416A1 (en) * 2012-05-29 2015-03-12 Korea Research Institute Of Bioscience And Biotech Metabolite for improving production, maintenance and proliferation of pluripotent stem cells, composition comprising the same, and method of culturing pluripotent stem cell using the same
US20140220680A1 (en) * 2013-02-04 2014-08-07 Royan Research Xeno-free and a feeder free self-renewal extracellular matrix for long-term maintenance of undifferentiated human pluripotent stem cells and method of synthesizing the same
EP2966163B1 (en) 2013-03-06 2018-01-17 Kyoto University Culture system for pluripotent stem cells and method for subculturing pluripotent stem cells
US8859286B2 (en) * 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
JP2016527878A (en) 2013-06-14 2016-09-15 ザ ユニバーシティー オブ クイーンズランド Renal progenitor cells
JP6998772B2 (en) * 2015-04-09 2022-02-04 ビオラミナ アーベー Methods and Compositions for Producing Stem Cell-Derived Dopaminergic Cells for Use in the Treatment of Neurodegenerative Diseases
US11230696B2 (en) 2015-07-29 2022-01-25 Hadasit Medical Research Services And Development Ltd. Large scale production of retinal pigment epithelial cells
AU2017335841A1 (en) 2016-09-28 2019-04-04 Organovo, Inc. Use of engineered renal tissues in assays
CN112899222A (en) * 2021-03-17 2021-06-04 广州暨大美塑生物科技有限公司 Efficient cell serum-free culture medium system and application of cell secretion thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050037492A1 (en) * 2000-01-11 2005-02-17 Chunhui Xu Medium for growing human embryonic stem cells
US20050196864A1 (en) * 2004-02-10 2005-09-08 Goldman Steven A. Induction and high-yield preparative purification of mesencephalic dopaminergic neuronal progenitor cells and dopaminergic neurons from human embryonic stem cells
US20060073591A1 (en) * 2004-01-09 2006-04-06 Abitorabi M A Cell culture media
US20060078543A1 (en) * 2000-03-14 2006-04-13 Reubinoff Benjamin E Neural progenitor cells derived from embryonic stem cells
US20060211109A1 (en) * 2003-03-12 2006-09-21 Reliance Life Sciences Pvt. Ltd. Derivation of terminally differentiated dopaminergic neurons from human embryonic stem cells

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US6180404B1 (en) 1996-04-09 2001-01-30 The Board Of Trustees Of Southern Illinois University Cultural medium for maintaining neural cells in ambient atmosphere
JP2001508302A (en) 1997-01-10 2001-06-26 ライフ テクノロジーズ，インコーポレイテッド Embryonic stem cell serum replacement
TR199902432T2 (en) 1997-04-04 2000-01-21 Prizer Products Inc. Nicotinamide is highly valued.
GB9715584D0 (en) 1997-07-23 1997-10-01 Eisai Co Ltd Compounds
GB0001930D0 (en) 2000-01-27 2000-03-22 Novartis Ag Organic compounds
IL156413A0 (en) 2001-01-31 2004-01-04 Pfizer Prod Inc Nicotinamide biaryl derivatives useful as inhibitors of pde4 isozymes
CA2461859A1 (en) 2001-09-28 2003-04-10 Es Cell International Pte Ltd Methods of derivation and propagation of undifferentiated human embryonic stem (hes) cells on feeder-free matrices and human feeder layers
AU2003201745A1 (en) 2002-02-11 2003-09-04 Pfizer Limited Nicotinamide derivatives and a tiotropium salt in combination for the treatment of e.g. inflammatory, allergic and respiratory diseases
US7736892B2 (en) 2002-02-25 2010-06-15 Kansas State University Research Foundation Cultures, products and methods using umbilical cord matrix cells
US20030161818A1 (en) 2002-02-25 2003-08-28 Kansas State University Research Foundation Cultures, products and methods using stem cells
GB0206309D0 (en) 2002-03-16 2002-05-01 Axordia Ltd Isolated cells
GB2407821B (en) 2002-06-05 2007-03-21 Es Cell Int Pte Ltd Stem Cells
US7267981B2 (en) 2002-10-07 2007-09-11 Technion Research & Development Foundation Ltd. Human foreskin fibroblasts for culturing ES cells
AU2003285172A1 (en) 2002-11-08 2004-06-03 The Johns Hopkins University Human embryonic stem cell cultures, and compositions and methods for growing same
US7288658B2 (en) 2003-07-15 2007-10-30 Hoffmann-La Roche Inc. Process for preparation of pyridine derivatives
TWI280280B (en) 2003-12-09 2007-05-01 Ind Tech Res Inst Culture system and method for expansion and undifferentiated growth of human embryonic stem cells
EP1730261A4 (en) 2004-03-10 2007-11-28 Univ California Compositions and methods for growth of embryonic stem cells
WO2006070370A2 (en) 2004-12-29 2006-07-06 Hadasit Medical Research Services & Development Limited Stem cells culture systems
WO2007002086A2 (en) 2005-06-22 2007-01-04 Geron Corporation Suspension culture of human embryonic stem cells
JP5395058B2 (en) 2007-04-18 2014-01-22 ハダシット メディカル リサーチ サーヴィシーズ アンド ディヴェロップメント リミテッド Retinal pigment epithelial cells derived from stem cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050037492A1 (en) * 2000-01-11 2005-02-17 Chunhui Xu Medium for growing human embryonic stem cells
US20060078543A1 (en) * 2000-03-14 2006-04-13 Reubinoff Benjamin E Neural progenitor cells derived from embryonic stem cells
US20060211109A1 (en) * 2003-03-12 2006-09-21 Reliance Life Sciences Pvt. Ltd. Derivation of terminally differentiated dopaminergic neurons from human embryonic stem cells
US20060073591A1 (en) * 2004-01-09 2006-04-06 Abitorabi M A Cell culture media
US20050196864A1 (en) * 2004-02-10 2005-09-08 Goldman Steven A. Induction and high-yield preparative purification of mesencephalic dopaminergic neuronal progenitor cells and dopaminergic neurons from human embryonic stem cells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2142641A4 *

Also Published As

Publication number Publication date
US8597947B2 (en) 2013-12-03
EP2142641A2 (en) 2010-01-13
US20120122209A1 (en) 2012-05-17
EP2142641A4 (en) 2010-08-18
US20070212777A1 (en) 2007-09-13
US8940537B2 (en) 2015-01-27
US20100093091A1 (en) 2010-04-15
WO2008120218A2 (en) 2008-10-09

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