WO2009087681A3 - Methods for characterisation of mammalian embryonic stem cells by multiplex pcr - Google Patents

Methods for characterisation of mammalian embryonic stem cells by multiplex pcr Download PDF

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Publication number
WO2009087681A3
WO2009087681A3 PCT/IN2008/000868 IN2008000868W WO2009087681A3 WO 2009087681 A3 WO2009087681 A3 WO 2009087681A3 IN 2008000868 W IN2008000868 W IN 2008000868W WO 2009087681 A3 WO2009087681 A3 WO 2009087681A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cells
embryonic stem
characterisation
methods
multiplex pcr
Prior art date
Application number
PCT/IN2008/000868
Other languages
French (fr)
Other versions
WO2009087681A2 (en
Inventor
Krishna Murali
Pal Rajarshi
Khanna Aparna
Original Assignee
Reliance Life Sciences Pvt. Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Reliance Life Sciences Pvt. Ltd. filed Critical Reliance Life Sciences Pvt. Ltd.
Publication of WO2009087681A2 publication Critical patent/WO2009087681A2/en
Publication of WO2009087681A3 publication Critical patent/WO2009087681A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Abstract

The present disclosure relates to a rapid, cost effective, robust and sensitive method for routine testing of embryonic stem cells. The present disclosure in particular provides a simple inexpensive and definitive multitasked semi-quantitative multiplex RT-PCR system for human ES cell characterization.
PCT/IN2008/000868 2007-12-28 2008-12-26 Methods for characterisation of mammalian embryonic stem cells by multiplex pcr WO2009087681A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2594MU2007 2007-12-28
IN2594/MUM/2007 2007-12-28

Publications (2)

Publication Number Publication Date
WO2009087681A2 WO2009087681A2 (en) 2009-07-16
WO2009087681A3 true WO2009087681A3 (en) 2009-09-11

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2008/000868 WO2009087681A2 (en) 2007-12-28 2008-12-26 Methods for characterisation of mammalian embryonic stem cells by multiplex pcr

Country Status (1)

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WO (1) WO2009087681A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111575387A (en) * 2020-05-29 2020-08-25 云南中科胚胎工程生物技术有限公司 Method for identifying sex of early embryo of Dorper sheep

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060286544A1 (en) * 2005-05-17 2006-12-21 Arundhati Mandal Establishment of a human embryonic stem cell line using mammalian cells
US20070048757A1 (en) * 2005-05-31 2007-03-01 Applera Corporation Methods for characterizing cells using amplified micro rnas

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060286544A1 (en) * 2005-05-17 2006-12-21 Arundhati Mandal Establishment of a human embryonic stem cell line using mammalian cells
US20070048757A1 (en) * 2005-05-31 2007-03-01 Applera Corporation Methods for characterizing cells using amplified micro rnas

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CLAYTON HELEN ET AL: "Growth and differentiation of progenitor/stem cells derived from the human mammary gland", EXPERIMENTAL CELL RESEARCH, ACADEMIC PRESS, US, vol. 297, no. 2, 30 April 2004 (2004-04-30), pages 444 - 460, XP002372634, ISSN: 0014-4827 *
HARTSHORN C ET AL: "Single-cell duplex RT-LATE-PCR reveals Oct4 and Xist RNA gradients in 8-cell embryos", BMC BIOTECHNOLOGY 20071207 BIOMED CENTRAL LTD.; CURRENT SCIENCE GROUP; MIDDLESEX HOUSE GB, vol. 7, 7 December 2007 (2007-12-07), XP002534226 *
QUENARD A ET AL: "Deleterious mutations in exon 1 of MECP2 in Rett syndrome", EUROPEAN JOURNAL OF MEDICAL GENETICS, ELSEVIER, NL, vol. 49, no. 4, 1 July 2006 (2006-07-01), pages 313 - 322, XP024920057, ISSN: 1769-7212, [retrieved on 20060701] *
SANZ EVA ET AL: "Human cord blood CD34+Pax-5+ B-cell progenitors: Single-cell analyses of their gene expression profiles.", BLOOD, vol. 101, no. 9, May 2003 (2003-05-01), pages 3424 - 3430, XP002534227, ISSN: 0006-4971 *
TANG FUCHOU ET AL: "220-plex microRNA expression profile of a single cell.", NATURE PROTOCOLS 2006, vol. 1, no. 3, 2006, pages 1154 - 1159, XP001539381, ISSN: 1750-2799 *
TANG FUCHOU ET AL: "MicroRNA expression profiling of single whole embryonic stem cells", NUCLEIC ACIDS RESEARCH, vol. 34, no. 2, 2006, XP002534225, ISSN: 0305-1048 *

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Publication number Publication date
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