WO2009105570A3 - Methods, surface modified plates and compositions for cell attachment, cultivation and detachment - Google Patents

Methods, surface modified plates and compositions for cell attachment, cultivation and detachment Download PDF

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Publication number
WO2009105570A3
WO2009105570A3 PCT/US2009/034558 US2009034558W WO2009105570A3 WO 2009105570 A3 WO2009105570 A3 WO 2009105570A3 US 2009034558 W US2009034558 W US 2009034558W WO 2009105570 A3 WO2009105570 A3 WO 2009105570A3
Authority
WO
WIPO (PCT)
Prior art keywords
detachment
cultivation
compositions
cell attachment
methods
Prior art date
Application number
PCT/US2009/034558
Other languages
French (fr)
Other versions
WO2009105570A2 (en
Inventor
Benjamin Fryer
Shelley Nelson
Villy Nielsen
Thomas Brevig
Tina Kristensen Marwood
Original Assignee
Centocor Ortho Biotech Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP2010547755A priority Critical patent/JP5733986B2/en
Priority to CA2715878A priority patent/CA2715878C/en
Application filed by Centocor Ortho Biotech Inc. filed Critical Centocor Ortho Biotech Inc.
Priority to RU2010138801/10A priority patent/RU2551772C2/en
Priority to AU2009215516A priority patent/AU2009215516B2/en
Priority to CN2009801061086A priority patent/CN102046779A/en
Priority to MX2010009251A priority patent/MX2010009251A/en
Priority to KR1020167036144A priority patent/KR20170001727A/en
Priority to BRPI0908033-3A priority patent/BRPI0908033A2/en
Priority to KR1020177033213A priority patent/KR102026622B1/en
Priority to KR1020197014456A priority patent/KR20190057164A/en
Priority to BR122017025207-0A priority patent/BR122017025207B1/en
Priority to KR1020167003806A priority patent/KR101731474B1/en
Priority to EP09712342.6A priority patent/EP2254990B1/en
Publication of WO2009105570A2 publication Critical patent/WO2009105570A2/en
Publication of WO2009105570A3 publication Critical patent/WO2009105570A3/en
Priority to AU2014256400A priority patent/AU2014256400B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material

Abstract

The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.
PCT/US2009/034558 2008-02-21 2009-02-19 Methods, surface modified plates and compositions for cell attachment, cultivation and detachment WO2009105570A2 (en)

Priority Applications (14)

Application Number Priority Date Filing Date Title
KR1020177033213A KR102026622B1 (en) 2008-02-21 2009-02-19 Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
BRPI0908033-3A BRPI0908033A2 (en) 2008-02-21 2009-02-19 Surface modified plate method and compositions for cell adhesion, culture and detachment
RU2010138801/10A RU2551772C2 (en) 2008-02-21 2009-02-19 Methods, surface-modified carriers and compositions for immobilisation, cultivation and cell detachment
CA2715878A CA2715878C (en) 2008-02-21 2009-02-19 Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
CN2009801061086A CN102046779A (en) 2008-02-21 2009-02-19 Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
MX2010009251A MX2010009251A (en) 2008-02-21 2009-02-19 Methods, surface modified plates and compositions for cell attachment, cultivation and detachment.
KR1020197014456A KR20190057164A (en) 2008-02-21 2009-02-19 Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
JP2010547755A JP5733986B2 (en) 2008-02-21 2009-02-19 Methods, surface modified plates, and compositions for cell attachment, culture and detachment
AU2009215516A AU2009215516B2 (en) 2008-02-21 2009-02-19 Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
KR1020167036144A KR20170001727A (en) 2008-02-21 2009-02-19 Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
BR122017025207-0A BR122017025207B1 (en) 2008-02-21 2009-02-19 surface that is part of a container or matrix intended for use in a cell culture or analysis, devoid of a layer of feeder cells and devoid of an adsorbent layer
KR1020167003806A KR101731474B1 (en) 2008-02-21 2009-02-19 Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
EP09712342.6A EP2254990B1 (en) 2008-02-21 2009-02-19 Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
AU2014256400A AU2014256400B2 (en) 2008-02-21 2014-10-31 Methods, surface modified plates and compositions for cell attachment, cultivation and detachment

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3054408P 2008-02-21 2008-02-21
US61/030,544 2008-02-21

Publications (2)

Publication Number Publication Date
WO2009105570A2 WO2009105570A2 (en) 2009-08-27
WO2009105570A3 true WO2009105570A3 (en) 2010-04-15

Family

ID=40651830

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/034558 WO2009105570A2 (en) 2008-02-21 2009-02-19 Methods, surface modified plates and compositions for cell attachment, cultivation and detachment

Country Status (11)

Country Link
US (2) US10066203B2 (en)
EP (1) EP2254990B1 (en)
JP (1) JP5733986B2 (en)
KR (5) KR20190057164A (en)
CN (2) CN102046779A (en)
AU (1) AU2009215516B2 (en)
BR (2) BRPI0908033A2 (en)
CA (2) CA2715878C (en)
MX (1) MX2010009251A (en)
RU (1) RU2551772C2 (en)
WO (1) WO2009105570A2 (en)

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