WO2010111690A3 - Polymerase compositions and methods - Google Patents

Polymerase compositions and methods Download PDF

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Publication number
WO2010111690A3
WO2010111690A3 PCT/US2010/028972 US2010028972W WO2010111690A3 WO 2010111690 A3 WO2010111690 A3 WO 2010111690A3 US 2010028972 W US2010028972 W US 2010028972W WO 2010111690 A3 WO2010111690 A3 WO 2010111690A3
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WO
WIPO (PCT)
Prior art keywords
altered
disclosed
compositions
properties
methods
Prior art date
Application number
PCT/US2010/028972
Other languages
French (fr)
Other versions
WO2010111690A2 (en
Inventor
Stephen P. Hendricks
Michael Phelan
Marian Peris
Cheng-Yao Chen
Daniel Mazur
Xinzhan Peng
Amy Castillo
Original Assignee
Life Technologies Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Life Technologies Corporation filed Critical Life Technologies Corporation
Publication of WO2010111690A2 publication Critical patent/WO2010111690A2/en
Publication of WO2010111690A3 publication Critical patent/WO2010111690A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching

Abstract

Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.
PCT/US2010/028972 2009-03-27 2010-03-26 Polymerase compositions and methods WO2010111690A2 (en)

Applications Claiming Priority (22)

Application Number Priority Date Filing Date Title
US16432409P 2009-03-27 2009-03-27
US61/164,324 2009-03-27
US18477009P 2009-06-05 2009-06-05
US61/184,770 2009-06-05
US24277109P 2009-09-15 2009-09-15
US61/242,771 2009-09-15
US24545709P 2009-09-24 2009-09-24
US61/245,457 2009-09-24
US26397409P 2009-11-24 2009-11-24
US61/263,974 2009-11-24
US28938809P 2009-12-22 2009-12-22
US61/289,388 2009-12-22
US29361610P 2010-01-08 2010-01-08
US29361810P 2010-01-08 2010-01-08
US61/293,616 2010-01-08
US61/293,618 2010-01-08
US29991910P 2010-01-29 2010-01-29
US29991710P 2010-01-29 2010-01-29
US61/299,917 2010-01-29
US61/299,919 2010-01-29
US30735610P 2010-02-23 2010-02-23
US61/307,356 2010-02-23

Publications (2)

Publication Number Publication Date
WO2010111690A2 WO2010111690A2 (en) 2010-09-30
WO2010111690A3 true WO2010111690A3 (en) 2011-03-31

Family

ID=42781937

Family Applications (4)

Application Number Title Priority Date Filing Date
PCT/US2010/028972 WO2010111690A2 (en) 2009-03-27 2010-03-26 Polymerase compositions and methods
PCT/US2010/028952 WO2010111674A2 (en) 2009-03-27 2010-03-26 Methods and apparatus for single molecule sequencing using energy transfer detection
PCT/US2010/028967 WO2010111686A2 (en) 2009-03-27 2010-03-26 Labeled enzyme compositions, methods & systems
PCT/US2010/028974 WO2010111691A2 (en) 2009-03-27 2010-03-26 Conjugates of biomolecules to nanoparticles

Family Applications After (3)

Application Number Title Priority Date Filing Date
PCT/US2010/028952 WO2010111674A2 (en) 2009-03-27 2010-03-26 Methods and apparatus for single molecule sequencing using energy transfer detection
PCT/US2010/028967 WO2010111686A2 (en) 2009-03-27 2010-03-26 Labeled enzyme compositions, methods & systems
PCT/US2010/028974 WO2010111691A2 (en) 2009-03-27 2010-03-26 Conjugates of biomolecules to nanoparticles

Country Status (2)

Country Link
US (20) US20110014612A1 (en)
WO (4) WO2010111690A2 (en)

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US8603792B2 (en) 2009-03-27 2013-12-10 Life Technologies Corporation Conjugates of biomolecules to nanoparticles

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US8603792B2 (en) 2013-12-10
US10093973B2 (en) 2018-10-09
US20190062828A1 (en) 2019-02-28
US10093974B2 (en) 2018-10-09
US20160265045A1 (en) 2016-09-15
US11008612B2 (en) 2021-05-18
US20130040363A1 (en) 2013-02-14
US10093972B2 (en) 2018-10-09
US20160265044A1 (en) 2016-09-15
US11015220B2 (en) 2021-05-25
US20170191051A1 (en) 2017-07-06
US9932573B2 (en) 2018-04-03
US9567629B2 (en) 2017-02-14
US20160186243A1 (en) 2016-06-30
WO2010111691A3 (en) 2011-03-31
WO2010111674A3 (en) 2011-05-12
US20140308730A1 (en) 2014-10-16

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