WO2010141391A3 - Mutant dna polymerases - Google Patents

Mutant dna polymerases Download PDF

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Publication number
WO2010141391A3
WO2010141391A3 PCT/US2010/036760 US2010036760W WO2010141391A3 WO 2010141391 A3 WO2010141391 A3 WO 2010141391A3 US 2010036760 W US2010036760 W US 2010036760W WO 2010141391 A3 WO2010141391 A3 WO 2010141391A3
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WO
WIPO (PCT)
Prior art keywords
mutant dna
dna polymerases
mutant
polymerases
nucleotides
Prior art date
Application number
PCT/US2010/036760
Other languages
French (fr)
Other versions
WO2010141391A2 (en
Inventor
Marian Peris
Michael Phelan
Barnett B. Rosenblum
Stephen P. Hendricks
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Life Technologies Corporation
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Application filed by Life Technologies Corporation filed Critical Life Technologies Corporation
Publication of WO2010141391A2 publication Critical patent/WO2010141391A2/en
Publication of WO2010141391A3 publication Critical patent/WO2010141391A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6872—Methods for sequencing involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Abstract

Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.
PCT/US2010/036760 2009-06-05 2010-05-28 Mutant dna polymerases WO2010141391A2 (en)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US18477409P 2009-06-05 2009-06-05
US61/184,774 2009-06-05
US24276209P 2009-09-15 2009-09-15
US61/242,762 2009-09-15
US26332009P 2009-11-20 2009-11-20
US61/263,320 2009-11-20
US29553310P 2010-01-15 2010-01-15
US61/295,533 2010-01-15

Publications (2)

Publication Number Publication Date
WO2010141391A2 WO2010141391A2 (en) 2010-12-09
WO2010141391A3 true WO2010141391A3 (en) 2011-04-21

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2010/036755 WO2010141390A2 (en) 2009-06-05 2010-05-28 Nucleotide transient binding for sequencing methods
PCT/US2010/036760 WO2010141391A2 (en) 2009-06-05 2010-05-28 Mutant dna polymerases

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PCT/US2010/036755 WO2010141390A2 (en) 2009-06-05 2010-05-28 Nucleotide transient binding for sequencing methods

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US (11) US20100311144A1 (en)
WO (2) WO2010141390A2 (en)

Families Citing this family (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010111674A2 (en) 2009-03-27 2010-09-30 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
US20100311144A1 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Mutant dna polymerases
WO2011090745A1 (en) 2009-12-29 2011-07-28 Life Technologies Corporation Single molecule detection and sequencing using fluorescence lifetime imaging
US9353412B2 (en) 2010-06-18 2016-05-31 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
US8986930B2 (en) * 2010-07-12 2015-03-24 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
AU2011312218B2 (en) 2010-10-04 2015-11-05 Sequencing Health, Inc. Systems and methods for automated reusable parallel biological reactions
US20120252682A1 (en) * 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
JP6193252B2 (en) 2011-12-01 2017-09-06 ジナプシス インコーポレイテッド System and method for high efficiency electronic sequencing and detection
US9279154B2 (en) * 2011-12-21 2016-03-08 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
US20140004562A1 (en) * 2012-01-20 2014-01-02 East China University Of Science And Technology Reversibly water-soluble nanocrystals
KR101545848B1 (en) * 2012-04-09 2015-08-21 (주)바이오니아 High-sensitivity nucleic acid preparation methods for the detection of nucleic acid by nucleic acid polymerization
WO2013185137A1 (en) 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
WO2014152625A1 (en) 2013-03-15 2014-09-25 Genapsys, Inc. Systems and methods for biological analysis
US10605734B2 (en) 2013-04-03 2020-03-31 Life Technologies Corporation Systems and methods for genetic sequencing
US9546398B2 (en) * 2013-11-14 2017-01-17 Agilent Technologies, Inc. Polymerase idling method for single molecule DNA sequencing
WO2015089238A1 (en) 2013-12-11 2015-06-18 Genapsys, Inc. Systems and methods for biological analysis and computation
EP3132060B1 (en) 2014-04-18 2019-03-13 Genapsys Inc. Methods and systems for nucleic acid amplification
WO2016154215A1 (en) * 2015-03-23 2016-09-29 The Trustees Of Columbia University In The City Of New York Polymer tagged nucleotides for single molecule electronic snp assay
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
WO2017087724A1 (en) 2015-11-17 2017-05-26 Omniome, Inc. Methods for determining sequence profiles
US20170160187A1 (en) * 2015-12-08 2017-06-08 Tdk Corporation Laser Scanner Particle Counter and Imager
US11384391B2 (en) 2016-04-04 2022-07-12 The Trustees Of Columbia University In The City Of New York Flourescene energy transfer-based single molecule/ensemble DNA sequencing by synthesis
US11326206B2 (en) * 2016-04-07 2022-05-10 Pacific Biosciences Of California, Inc. Methods of quantifying target nucleic acids and identifying sequence variants
JP2019512209A (en) * 2016-04-22 2019-05-16 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． Nucleic acid sequencing method and system utilizing enhanced detection of nucleotide specific ternary complex formation
CA3022431C (en) * 2016-04-29 2023-03-21 Omniome, Inc. Method of nucleic acid sequence determination
CA3021769C (en) 2016-04-29 2021-11-23 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
US10597643B2 (en) 2016-04-29 2020-03-24 Omniome, Inc. Polymerases engineered to reduce nucleotide-independent DNA binding
CN116397014A (en) 2016-07-20 2023-07-07 测序健康公司 Systems and methods for nucleic acid sequencing
US10443098B2 (en) * 2016-08-15 2019-10-15 Omniome, Inc. Method and system for sequencing nucleic acids
WO2018034780A1 (en) 2016-08-15 2018-02-22 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
CA3040924A1 (en) * 2016-12-09 2018-06-14 Regeneron Pharmaceuticals, Inc. Systems and methods for sequencing t cell receptors and uses thereof
DE102016124692B4 (en) * 2016-12-16 2019-05-16 Gna Biosolutions Gmbh Method and system for amplifying a nucleic acid
WO2018125759A1 (en) * 2016-12-30 2018-07-05 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
AU2018208462B2 (en) * 2017-01-10 2021-07-29 Pacific Biosciences Of California, Inc. Polymerases engineered to reduce nucleotide-independent DNA binding
SG11201906567YA (en) * 2017-01-20 2019-08-27 Omniome Inc Allele-specific capture of nucleic acids
US9932631B1 (en) 2017-09-11 2018-04-03 Omniome, Inc. Genotyping by polymerase binding
EP3571319A1 (en) * 2017-01-20 2019-11-27 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
CA3050852C (en) * 2017-01-20 2021-03-09 Omniome, Inc. Genotyping by polymerase binding
EP3580350B1 (en) 2017-02-13 2020-11-18 Qiagen Sciences, LLC Polymerase enzyme from pyrococcus furiosus
EP3580351A1 (en) 2017-02-13 2019-12-18 Qiagen Sciences, LLC Polymerase enzyme from 9°n
EP3580334A1 (en) * 2017-02-13 2019-12-18 QIAGEN GmbH Polymerase enzyme from phage t4
WO2018148726A1 (en) * 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from phage t4
WO2018152162A1 (en) 2017-02-15 2018-08-23 Omniome, Inc. Distinguishing sequences by detecting polymerase dissociation
US11249161B2 (en) 2017-03-03 2022-02-15 The Johns Hopkins University Magnetic resonance method for detecting molecular binding with enhanced sensitivity
WO2018187013A1 (en) 2017-04-04 2018-10-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
US10883092B2 (en) * 2017-04-18 2021-01-05 Mgi Tech Co., Ltd. Phi29 DNA polymerase and encoding gene and application thereof
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
CA3076378A1 (en) 2017-09-21 2019-03-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
WO2019060830A1 (en) 2017-09-25 2019-03-28 Plexium, Inc. Oligonucleotide encoded chemical libraries
AU2018353136B2 (en) 2017-10-19 2022-05-19 Pacific Biosciences Of California, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
US10851131B2 (en) 2018-02-16 2020-12-01 Illumina, Inc. Charge-tagged nucleotides and methods of use thereof
WO2019161253A1 (en) 2018-02-16 2019-08-22 Ultima Genomics, Inc. Methods for sequencing with single frequency detection
WO2019209426A1 (en) 2018-04-26 2019-10-31 Omniome, Inc. Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
EP3802874A1 (en) 2018-05-31 2021-04-14 Omniome, Inc. Increased signal to noise in nucleic acid sequencing
US11180794B2 (en) 2018-05-31 2021-11-23 Omniome, Inc. Methods and compositions for capping nucleic acids
AU2019312152A1 (en) 2018-07-24 2021-02-18 Pacific Biosciences Of California, Inc. Serial formation of ternary complex species
GB2574746B (en) 2018-09-17 2020-06-03 Omniome Inc Engineered polymerases for improved sequencing
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
US10876148B2 (en) 2018-11-14 2020-12-29 Element Biosciences, Inc. De novo surface preparation and uses thereof
US10768173B1 (en) 2019-09-06 2020-09-08 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US20240011086A1 (en) 2018-11-15 2024-01-11 Omniome, Inc. Electronic detection of nucleic acid structure
AU2019392932B2 (en) 2018-12-07 2023-11-02 Element Biosciences, Inc. Flow cell device and use thereof
US20220340887A1 (en) * 2019-09-18 2022-10-27 SequLITE Genomics US, Inc. Polymerases
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
CN114599795A (en) 2019-10-18 2022-06-07 加利福尼亚太平洋生物科学股份有限公司 Methods and compositions for capping nucleic acids
US20210139867A1 (en) 2019-11-08 2021-05-13 Omniome, Inc. Engineered polymerases for improved sequencing by binding
CA3165571C (en) 2019-12-23 2023-02-07 Singular Genomics Systems, Inc. Methods for long read sequencing
CN115667507A (en) 2019-12-31 2023-01-31 奇异基因组学系统公司 Polynucleotide barcodes for long read sequencing
AU2021230282A1 (en) 2020-03-03 2022-09-22 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
CN115836135A (en) 2020-05-05 2023-03-21 加利福尼亚太平洋生物科学股份有限公司 Compositions and methods for modifying polymerase-nucleic acid complexes
US11198121B1 (en) * 2020-06-10 2021-12-14 Element Biosciences, Inc. Flow cell systems and devices
US20210403993A1 (en) * 2020-06-30 2021-12-30 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless dna
WO2022072393A1 (en) * 2020-09-29 2022-04-07 University Of Washington Use of a double-stranded dna cytosine deaminase for mapping dna-protein interactions
WO2022094332A1 (en) 2020-10-30 2022-05-05 Element Biosciences, Inc. Reagents for massively parallel nucleic acid sequencing
US20240084378A1 (en) 2022-05-11 2024-03-14 10X Genomics, Inc. Compositions and methods for in situ sequencing

Family Cites Families (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5151507A (en) 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5198543A (en) * 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5001050A (en) * 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
US5412087A (en) 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
FR2703052B1 (en) * 1993-03-26 1995-06-02 Pasteur Institut New method of nucleic acid sequencing.
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US6482590B1 (en) * 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
US5773308A (en) 1997-02-10 1998-06-30 The United States Of America As Represented By The Secretary Of The Navy Photoactivatable o-nitrobenzyl polyethylene glycol-silane for the production of patterned biomolecular arrays
JP2002537858A (en) * 1999-03-10 2002-11-12 エーエスエム サイエンティフィック， インコーポレイテッド Methods for direct sequencing of nucleic acids
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6492161B1 (en) * 1999-06-02 2002-12-10 Prokaria Ltd. Bacteriophage RM 378 of a thermophilic host organism
US6982146B1 (en) * 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
WO2001023610A2 (en) * 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
US6309836B1 (en) * 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
CN100462433C (en) * 2000-07-07 2009-02-18 维西根生物技术公司 Real-time sequence determination
US7041812B2 (en) * 2001-08-29 2006-05-09 Amersham Biosciences Corp Labeled nucleoside polyphosphates
US7052839B2 (en) * 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use
US7223541B2 (en) * 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
US7057026B2 (en) * 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
AU2003259350A1 (en) * 2002-08-23 2004-03-11 Solexa Limited Modified nucleotides for polynucleotide sequencing
US7454424B2 (en) * 2003-01-16 2008-11-18 Hewlett-Packard Development Company, L.P. System and method for efficiently replicating a file
DE60335144D1 (en) * 2003-02-05 2011-01-05 Ge Healthcare Bio Sciences nucleic acid amplification
EP1592779A4 (en) * 2003-02-05 2007-12-12 Ge Healthcare Bio Sciences Terminal-phosphate-labeled nucleotides with new linkers
WO2004074503A2 (en) * 2003-02-21 2004-09-02 Hoser Mark J Nucleic acid sequencing methods, kits and reagents
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP1716254B1 (en) * 2004-02-19 2010-04-07 Helicos Biosciences Corporation Methods for analyzing polynucleotide sequences
US6883246B1 (en) * 2004-03-29 2005-04-26 Phillip A. Latham Freeboard measuring device
US8071755B2 (en) * 2004-05-25 2011-12-06 Helicos Biosciences Corporation Nucleotide analogs
US20080103053A1 (en) 2005-11-22 2008-05-01 Helicos Biosciences Corporation Methods and compositions for sequencing a nucleic acid
US20060003383A1 (en) 2004-06-07 2006-01-05 Applera Corporation Fluorogenic enzyme activity assay methods and compositions using fragmentable linkers
US7264934B2 (en) 2004-06-10 2007-09-04 Ge Healthcare Bio-Sciences Corp. Rapid parallel nucleic acid analysis
AU2005254984B2 (en) * 2004-06-10 2008-10-16 Ge Healthcare Bio-Sciences Corp. Method for nucleic acid analysis
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
CA2593855A1 (en) * 2005-01-31 2006-08-10 Pacific Biosciences Of California, Inc. Use of reversible extension terminator in nucleic acid sequencing
US7405281B2 (en) * 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
CN101346472B (en) * 2005-12-21 2013-04-24 霍夫曼—拉罗奇有限公司 Sequencing and genotyping using reversibly 2'-modified nucleotides
WO2007076057A2 (en) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
US20080269476A1 (en) * 2006-04-26 2008-10-30 Helicos Biosciences Corporation Molecules and methods for nucleic acid sequencing
WO2007147110A2 (en) 2006-06-16 2007-12-21 Pacific Biosciences Of California, Inc. Controlled initiation of primer extension
WO2008005673A2 (en) 2006-06-30 2008-01-10 Applera Corporation Reversible terminator nucleotides and methods of use
US20080091005A1 (en) 2006-07-20 2008-04-17 Visigen Biotechnologies, Inc. Modified nucleotides, methods for making and using same
WO2008051530A2 (en) * 2006-10-23 2008-05-02 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US7897737B2 (en) * 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US20080138804A1 (en) * 2006-12-12 2008-06-12 Helicos Biosciences Corporation Buffer composition
US7678894B2 (en) * 2007-05-18 2010-03-16 Helicos Biosciences Corporation Nucleotide analogs
US7960116B2 (en) * 2007-09-28 2011-06-14 Pacific Biosciences Of California, Inc. Nucleic acid sequencing methods and systems
US20090176233A1 (en) * 2007-12-05 2009-07-09 Pacific Biosciences Of California, Inc. Screening assays for polymerase enhancement
US20110165652A1 (en) 2008-01-14 2011-07-07 Life Technologies Corporation Compositions, methods and systems for single molecule sequencing
WO2009145828A2 (en) * 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc. Two slow-step polymerase enzyme systems and methods
ES2614078T3 (en) * 2008-03-31 2017-05-29 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8999676B2 (en) * 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
US8420366B2 (en) * 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
EP2326733A2 (en) * 2008-09-05 2011-06-01 Pacific Biosciences of California, Inc. Engineering polymerases and reaction conditions for modified incorporation properties
US8481264B2 (en) * 2008-09-19 2013-07-09 Pacific Biosciences Of California, Inc. Immobilized nucleic acid complexes for sequence analysis
WO2010111674A2 (en) * 2009-03-27 2010-09-30 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
US20100311144A1 (en) * 2009-06-05 2010-12-09 Life Technologies Corporation Mutant dna polymerases
US8715932B2 (en) * 2010-08-20 2014-05-06 Intel Corporation Nucleic acid sequencing
US9546398B2 (en) * 2013-11-14 2017-01-17 Agilent Technologies, Inc. Polymerase idling method for single molecule DNA sequencing

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK 28 March 1996 (1996-03-28), "'DNA polymerase [Enterobacteria phage RB 69 ]", Database accession no. AAA93077.1 *
JACEWICZ, A. ET AL.: "The roles of Tyr391 and Tyr619 in RB69 DNA polymerase r eplication fidelity", JOURNAL OF MOLECULAR BIOLOGY, vol. 368, no. 1, 3 February 2007 (2007-02-03), pages 18 - 29, XP005931377, DOI: doi:10.1016/j.jmb.2007.01.067 *
TRZEMECKA, A. ET AL.: "Different behaviors in vivo of mutations in the beta h airpin loop of the DNA polymerases of the closely related phages T4 and RB69", JOURNAL OF MOLECULAR BIOLOGY, vol. 389, no. 5, 3 May 2009 (2009-05-03), pages 797 - 807, XP026174217, DOI: doi:10.1016/j.jmb.2009.04.055 *
ZHANG, H. ET AL.: "The L561A substitution in the nascent base-pair binding po cket of RB69 DNA polymerase reduces base discrimination", BIOCHEMISTRY, vol. 45, no. 7, 21 February 2006 (2006-02-21), pages 2211 - 2220 *
ZHONG, X. ET AL.: "Characterization of a replicative DNA polymerase mutant wi th reduced fidelity and increased translesion synthesis capacity", NUCLEIC ACIDS RESEARCH, vol. 36, no. 12, 24 May 2008 (2008-05-24), pages 3892 - 3904 *

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