WO2011058558A3 - Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state - Google Patents
Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state Download PDFInfo
- Publication number
- WO2011058558A3 WO2011058558A3 PCT/IL2010/000937 IL2010000937W WO2011058558A3 WO 2011058558 A3 WO2011058558 A3 WO 2011058558A3 IL 2010000937 W IL2010000937 W IL 2010000937W WO 2011058558 A3 WO2011058558 A3 WO 2011058558A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- pluripotent stem
- stem cells
- methods
- undifferentiated state
- cells
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/36—Lipids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
- C12N2500/92—Medium free of human- or animal-derived components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/98—Xeno-free medium and culture conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/99—Serum-free medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Transplantation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Priority Applications (13)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP19210132.7A EP3633025B1 (en) | 2009-11-12 | 2010-11-11 | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
JP2012538468A JP6276918B2 (en) | 2009-11-12 | 2010-11-11 | Medium, cell culture and method for culturing pluripotent stem cells in undifferentiated state |
ES10829622T ES2779048T3 (en) | 2009-11-12 | 2010-11-11 | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
US13/508,991 US9018010B2 (en) | 2009-11-12 | 2010-11-11 | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
DK10829622.9T DK2499236T3 (en) | 2009-11-12 | 2010-11-11 | CULTURAL MEDIA, CELL CULTURES AND METHODS FOR CULTIVATING PLURIPOTENT STEM CELLS IN AN UNDIFFERENTIAL CONDITION |
EP10829622.9A EP2499236B1 (en) | 2009-11-12 | 2010-11-11 | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
CA2783437A CA2783437C (en) | 2009-11-12 | 2010-11-11 | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
EP22185625.5A EP4166652A1 (en) | 2009-11-12 | 2010-11-11 | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
IL219736A IL219736A (en) | 2009-11-12 | 2012-05-10 | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
US14/696,520 US10876094B2 (en) | 2009-11-12 | 2015-04-27 | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
IL252607A IL252607B (en) | 2009-11-12 | 2017-06-01 | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
US17/097,023 US20210079342A1 (en) | 2009-11-12 | 2020-11-13 | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
US17/841,700 US20220333066A1 (en) | 2009-11-12 | 2022-06-16 | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US27286009P | 2009-11-12 | 2009-11-12 | |
US61/272,860 | 2009-11-12 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US13/508,991 A-371-Of-International US9018010B2 (en) | 2009-11-12 | 2010-11-11 | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
US14/696,520 Continuation US10876094B2 (en) | 2009-11-12 | 2015-04-27 | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2011058558A2 WO2011058558A2 (en) | 2011-05-19 |
WO2011058558A3 true WO2011058558A3 (en) | 2011-10-13 |
Family
ID=43992162
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IL2010/000937 WO2011058558A2 (en) | 2009-11-12 | 2010-11-11 | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
Country Status (8)
Country | Link |
---|---|
US (4) | US9018010B2 (en) |
EP (3) | EP4166652A1 (en) |
JP (5) | JP6276918B2 (en) |
CA (3) | CA3080762A1 (en) |
DK (2) | DK2499236T3 (en) |
ES (2) | ES2779048T3 (en) |
IL (1) | IL252607B (en) |
WO (1) | WO2011058558A2 (en) |
Families Citing this family (40)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA3080762A1 (en) | 2009-11-12 | 2011-05-19 | Technion Research & Development Foundation Limited | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
CN107189979B (en) | 2010-03-01 | 2021-05-04 | 詹森生物科技公司 | Method for purifying cells derived from pluripotent stem cells |
BR112013002811A8 (en) | 2010-08-05 | 2020-01-28 | Wisconsin Alumni Res Found | simplified basic means for human pluripotent cell culture |
US9279107B2 (en) | 2010-08-05 | 2016-03-08 | Wisconsin Alumni Research Foundation | Simplified basic media for human pluripotent cell culture |
DK2614141T3 (en) | 2010-09-07 | 2019-09-09 | Technion Res & Dev Foundation | PRESENT PROCEDURES AND CULTIVATION MEDIA FOR CULTURING PLURIPOTENT STEM CELLS |
GB201117469D0 (en) * | 2011-10-10 | 2011-11-23 | Cell Guidance Systems Ltd | Culture media for pluripotent stem cells |
JP6466170B2 (en) * | 2011-10-17 | 2019-02-06 | ミネルバ バイオテクノロジーズ コーポレーション | Medium for stem cell growth and induction |
JP5999658B2 (en) | 2011-11-25 | 2016-09-28 | 国立大学法人京都大学 | Method for culturing pluripotent stem cells |
EP2788033B1 (en) | 2011-12-05 | 2017-05-31 | Factor Bioscience Inc. | Methods and products for transfecting cells |
RU2668798C2 (en) | 2011-12-22 | 2018-10-02 | Янссен Байотек, Инк. | Methods for in vitro of step-by-step differentiation of pluripotent cells |
RU2668814C2 (en) * | 2012-05-07 | 2018-10-02 | Янссен Байотек, Инк. | Methods of producing cells of definitive and pancreatic endoderm |
RU2018108090A (en) | 2012-12-31 | 2019-02-25 | Янссен Байотек, Инк. | SUSPENDING AND CLUSTERING OF HUMAN PLURIPOTENT CELLS FOR THEIR DIFFERENTIATION IN PANCREATIC ENDOCRINE CELLS |
RU2658488C2 (en) | 2012-12-31 | 2018-06-21 | Янссен Байотек, Инк. | Method for obtaining cells expressing markers characteristic for pancreatic endocrine cells |
US10370644B2 (en) | 2012-12-31 | 2019-08-06 | Janssen Biotech, Inc. | Method for making human pluripotent suspension cultures and cells derived therefrom |
US9738861B2 (en) | 2013-03-06 | 2017-08-22 | Kyoto University | Culture system for pluripotent stem cells and method for subculturing pluripotent stem cells |
EP3008172B1 (en) | 2013-06-11 | 2020-05-06 | Ncardia B.V. | Culture medium compositions for maturating cardiomyocytes derived from pluripotent mammalian stem cells |
EP3015551B1 (en) * | 2013-06-28 | 2019-03-06 | Kaneka Corporation | Method for screening for pluripotent stem cell growth-promoting factor |
WO2015037622A1 (en) * | 2013-09-10 | 2015-03-19 | 富士フイルム株式会社 | Culture method for pluripotent stem cells, culture kit, and medium for pluripotent stem cell culture |
CN105637086B (en) * | 2013-09-10 | 2019-07-12 | 富士胶片株式会社 | The culture kit and culture medium of multipotential stem cell used in the cultural method of multipotential stem cell, this method |
WO2015098111A1 (en) * | 2013-12-26 | 2015-07-02 | 国立大学法人広島大学 | METHOD FOR ESTABLISHING iPS CELLS AND METHOD FOR LONG-TERM MAINTENANCE OF STEM CELLS |
JP6516280B2 (en) * | 2013-12-26 | 2019-05-22 | 国立大学法人広島大学 | Method for establishing iPS cells and method for long-term maintenance of stem cells |
EP3604499A1 (en) * | 2014-03-04 | 2020-02-05 | Fate Therapeutics, Inc. | Improved reprogramming methods and cell culture platforms |
KR20160147800A (en) * | 2014-05-06 | 2016-12-23 | 에프. 호프만-라 로슈 아게 | Method for differentiation of pluripotent stem cells into cardiomyocytes |
US20150335010A1 (en) | 2014-05-21 | 2015-11-26 | Bourbon Corporation | Maintenance medium for primate pluripotent stem cells |
CN104388382B (en) * | 2014-11-21 | 2017-05-10 | 吉林大学 | Method for rapidly preparing Feeder cells by using stirring-type cell culturing bottle rolling machine |
US9944894B2 (en) | 2015-01-16 | 2018-04-17 | General Electric Company | Pluripotent stem cell expansion and passage using a rocking platform bioreactor |
CN108138130A (en) * | 2015-08-31 | 2018-06-08 | 爱平世股份有限公司 | The method of multipotential stem cell manufacture system and production induced multi-potent stem cell |
CA3001917A1 (en) | 2015-10-16 | 2017-04-20 | Fate Therapeutics, Inc. | Platform for the induction & maintenance of ground state pluripotency |
CA3030252A1 (en) * | 2016-07-19 | 2018-01-25 | Accellta Ltd. | Culture media for culturing pluripotent stem cells in suspension |
WO2018029656A2 (en) * | 2016-08-12 | 2018-02-15 | The University Of Chicago | Methods for making and using therapeutic exosomes |
JP6647545B2 (en) * | 2017-05-02 | 2020-02-14 | 剛士 田邊 | Pharmaceutical and cosmetic compositions |
EP3770249A4 (en) * | 2018-03-20 | 2021-04-21 | FUJIFILM Corporation | Method for three-dimensionally culturing pluripotent stem cells |
KR102482097B1 (en) * | 2019-12-09 | 2022-12-29 | 주식회사 대웅제약 | Method for Preparing Mesenchymal Stem Cells from Human Pluripotent Stem Cells and Mesenchymal Stem Cells Prepared by Thereof |
WO2021145319A1 (en) * | 2020-01-14 | 2021-07-22 | 味の素株式会社 | Cell culture medium composition |
CN111484970B (en) * | 2020-04-30 | 2022-09-16 | 广州再生医学与健康广东省实验室 | Serum-free and feeder-layer-free embryo and pluripotent stem cell culture medium with low protein content |
CN117795057A (en) * | 2021-06-09 | 2024-03-29 | 艾策尔塔公司 | Serum-free medium for suspension culture of pluripotent stem cells of mammalian livestock |
US11735303B2 (en) | 2021-06-22 | 2023-08-22 | David Haase | Apparatus and method for determining a composition of a replacement therapy treatment |
CN113403262A (en) * | 2021-06-23 | 2021-09-17 | 昆明理工大学 | Layer-free culture method for stem cells of cynomolgus monkeys |
WO2023053220A1 (en) * | 2021-09-28 | 2023-04-06 | 公益財団法人京都大学iPS細胞研究財団 | Method for producing pluripotent stem cells |
WO2024030501A2 (en) * | 2022-08-02 | 2024-02-08 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Bovine trophoblast stem cells and uses thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050233446A1 (en) * | 2003-12-31 | 2005-10-20 | Parsons Xuejun H | Defined media for stem cell culture |
WO2008015682A2 (en) * | 2006-08-02 | 2008-02-07 | Technion Research & Development Foundation Ltd. | Methods of expanding embryonic stem cells in a suspension culture |
US20090191159A1 (en) * | 2007-06-15 | 2009-07-30 | Kazuhiro Sakurada | Multipotent/pluripotent cells and methods |
Family Cites Families (190)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL154600B (en) | 1971-02-10 | 1977-09-15 | Organon Nv | METHOD FOR THE DETERMINATION AND DETERMINATION OF SPECIFIC BINDING PROTEINS AND THEIR CORRESPONDING BINDABLE SUBSTANCES. |
NL154598B (en) | 1970-11-10 | 1977-09-15 | Organon Nv | PROCEDURE FOR DETERMINING AND DETERMINING LOW MOLECULAR COMPOUNDS AND PROTEINS THAT CAN SPECIFICALLY BIND THESE COMPOUNDS AND TEST PACKAGING. |
NL154599B (en) | 1970-12-28 | 1977-09-15 | Organon Nv | PROCEDURE FOR DETERMINING AND DETERMINING SPECIFIC BINDING PROTEINS AND THEIR CORRESPONDING BINDABLE SUBSTANCES, AND TEST PACKAGING. |
US3901654A (en) | 1971-06-21 | 1975-08-26 | Biological Developments | Receptor assays of biologically active compounds employing biologically specific receptors |
US3853987A (en) | 1971-09-01 | 1974-12-10 | W Dreyer | Immunological reagent and radioimmuno assay |
US3867517A (en) | 1971-12-21 | 1975-02-18 | Abbott Lab | Direct radioimmunoassay for antigens and their antibodies |
NL171930C (en) | 1972-05-11 | 1983-06-01 | Akzo Nv | METHOD FOR DETERMINING AND DETERMINING BITES AND TEST PACKAGING. |
US3850578A (en) | 1973-03-12 | 1974-11-26 | H Mcconnell | Process for assaying for biologically active molecules |
US3935074A (en) | 1973-12-17 | 1976-01-27 | Syva Company | Antibody steric hindrance immunoassay with two antibodies |
US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
US4034074A (en) | 1974-09-19 | 1977-07-05 | The Board Of Trustees Of Leland Stanford Junior University | Universal reagent 2-site immunoradiometric assay using labelled anti (IgG) |
US3984533A (en) | 1975-11-13 | 1976-10-05 | General Electric Company | Electrophoretic method of detecting antigen-antibody reaction |
US4098876A (en) | 1976-10-26 | 1978-07-04 | Corning Glass Works | Reverse sandwich immunoassay |
US4879219A (en) | 1980-09-19 | 1989-11-07 | General Hospital Corporation | Immunoassay utilizing monoclonal high affinity IgM antibodies |
IL68218A (en) | 1983-03-23 | 1985-12-31 | Univ Ramot | Compositions for cartilage repair comprising embryonal chondrocytes |
US5011771A (en) | 1984-04-12 | 1991-04-30 | The General Hospital Corporation | Multiepitopic immunometric assay |
US4666828A (en) | 1984-08-15 | 1987-05-19 | The General Hospital Corporation | Test for Huntington's disease |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4801531A (en) | 1985-04-17 | 1989-01-31 | Biotechnology Research Partners, Ltd. | Apo AI/CIII genomic polymorphisms predictive of atherosclerosis |
US5272057A (en) | 1988-10-14 | 1993-12-21 | Georgetown University | Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase |
US5192659A (en) | 1989-08-25 | 1993-03-09 | Genetype Ag | Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes |
US5281521A (en) | 1992-07-20 | 1994-01-25 | The Trustees Of The University Of Pennsylvania | Modified avidin-biotin technique |
US5489304A (en) | 1994-04-19 | 1996-02-06 | Brigham & Women's Hospital | Method of skin regeneration using a collagen-glycosaminoglycan matrix and cultured epithelial autograft |
US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
US7410773B2 (en) | 1995-02-02 | 2008-08-12 | Ghazi Jaswinder Dhoot | Method of preparing an undifferentiated cell |
AU7243100A (en) | 1995-02-02 | 2001-05-03 | Tristem Trading (Cyprus) Limited | A method of preparing an undifferentiated cell |
JPH08308561A (en) * | 1995-05-16 | 1996-11-26 | Sumitomo Electric Ind Ltd | Serum-free medium for animal cell culture |
US5811297A (en) | 1996-03-07 | 1998-09-22 | Amba Biosciences, Llc | Immortalized hematopoietic cell lines, cell system thereof with stromal cells, in vitro, ex vivo and in vivo uses, & in vitro generation of dendritic cells and macrophages |
JP4307552B2 (en) | 1996-03-15 | 2009-08-05 | ミューニン コーポレイション | Extracellular matrix signaling molecules |
US6878544B2 (en) | 1996-04-19 | 2005-04-12 | Neurotech Sa | Retinal cell lines with extended life-span and their applications |
EP0986635A4 (en) | 1997-01-10 | 2001-11-07 | Life Technologies Inc | Embryonic stem cell serum replacement |
US6090622A (en) | 1997-03-31 | 2000-07-18 | The Johns Hopkins School Of Medicine | Human embryonic pluripotent germ cells |
US6331406B1 (en) | 1997-03-31 | 2001-12-18 | The John Hopkins University School Of Medicine | Human enbryonic germ cell and methods of use |
AU8284198A (en) | 1997-07-02 | 1999-01-25 | Board Of Regents, The University Of Texas System | P53 as a regulator of cell differentiation |
IL122818A0 (en) | 1997-07-10 | 1998-08-16 | Yeda Res & Dev | Chimeric interleukin-6 soluble receptor/ligand protein analogs thereof and uses thereof |
AU1197699A (en) | 1997-10-23 | 1999-05-10 | Geron Corporation | Methods and materials for the growth of primate-derived primordial stem cells |
US7410798B2 (en) | 2001-01-10 | 2008-08-12 | Geron Corporation | Culture system for rapid expansion of human embryonic stem cells |
US7413904B2 (en) | 1998-10-23 | 2008-08-19 | Geron Corporation | Human embryonic stem cells having genetic modifications |
US6667176B1 (en) | 2000-01-11 | 2003-12-23 | Geron Corporation | cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells |
WO2000027995A1 (en) | 1998-11-09 | 2000-05-18 | Monash University | Embryonic stem cells |
IL142914A0 (en) * | 1998-11-09 | 2002-04-21 | Consorzio Per La Gestione Del | Serum free medium for chondrocyte-like cells |
US20030129745A1 (en) | 1999-10-28 | 2003-07-10 | Robl James M. | Gynogenetic or androgenetic production of pluripotent cells and cell lines, and use thereof to produce differentiated cells and tissues |
US7455983B2 (en) | 2000-01-11 | 2008-11-25 | Geron Corporation | Medium for growing human embryonic stem cells |
JP2001229797A (en) | 2000-02-15 | 2001-08-24 | Yazaki Corp | Gas type breaker for source circuit |
WO2001068815A1 (en) | 2000-03-14 | 2001-09-20 | Es Cell International Pte Ltd | Embryonic stem cells and neural progenitor cells derived therefrom |
AU2001244354A1 (en) | 2000-03-30 | 2001-10-15 | Oxford Biomedica (Uk) Limited | Retinoic acid receptor beta-2, its antagonists, and gene therapy vectors for thetreatment of neurological disorders |
US7473555B2 (en) | 2000-04-27 | 2009-01-06 | Geron Corporation | Protocols for making hepatocytes from embryonic stem cells |
NL1017973C2 (en) | 2000-05-10 | 2002-11-08 | Tristem Trading Cyprus Ltd | Design. |
US7250294B2 (en) | 2000-05-17 | 2007-07-31 | Geron Corporation | Screening small molecule drugs using neural cells differentiated from human embryonic stem cells |
CN100580079C (en) | 2000-05-17 | 2010-01-13 | 杰龙公司 | Neural progenitor cell populations |
US20020090722A1 (en) | 2000-06-15 | 2002-07-11 | Tanja Dominko | Pluripotent mammalian cells |
GB0025088D0 (en) | 2000-10-13 | 2000-11-29 | Univ Edinburgh | Stem cells |
US6576464B2 (en) | 2000-11-27 | 2003-06-10 | Geron Corporation | Methods for providing differentiated stem cells |
CA2430653A1 (en) | 2000-11-27 | 2002-08-08 | Yissum Research Development Company Of The Hebrew University In Jerusalem | Transfection of human embryonic stem cells |
JP2004532648A (en) | 2000-11-30 | 2004-10-28 | ステムロン インコーポレイテッド | Isolated homozygous stem cells, differentiated cells derived therefrom, and materials and methods for making and using the same |
CA2447015A1 (en) | 2001-05-15 | 2002-11-21 | Rappaport Family Institute For Research In The Medical Sciences | Insulin producing cells derived from human embryonic stem cells |
CN101029302B (en) | 2001-06-21 | 2011-03-30 | 杰龙公司 | Dopaminergic neurons and proliferation-competent precursor cells for treating parkinson's disease |
CN1636054A (en) | 2001-07-06 | 2005-07-06 | 杰龙公司 | Mesenchymal cells and osteoblasts from human embryonic stem cell |
JP2004535199A (en) | 2001-07-12 | 2004-11-25 | ジェロン コーポレイション | Cardiomyocyte lineage cells produced from human pluripotent stem cells |
AU2002317039B2 (en) | 2001-07-24 | 2007-10-04 | Es Cell International Pte Ltd | Methods of inducing differentiation of stem cells |
US7432104B2 (en) | 2001-08-06 | 2008-10-07 | Bresgen Inc. | Methods for the culture of human embryonic stem cells on human feeder cells |
US20030211603A1 (en) | 2001-08-14 | 2003-11-13 | Earp David J. | Reprogramming cells for enhanced differentiation capacity using pluripotent stem cells |
WO2003018780A1 (en) | 2001-08-27 | 2003-03-06 | Advanced Cell Technology, Inc. | De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies |
WO2003023018A2 (en) | 2001-09-12 | 2003-03-20 | DeveloGen Aktiengesellschaft für entwicklungsbiologische Forschung | A method for isolating, culturing and differentiating intestinal stem cells for therapeutic use |
US20050095708A1 (en) | 2001-11-09 | 2005-05-05 | Pera Martin F. | Characterization and isolation of subsets of human embryonic stem cells (HES) and cells associated or derived therefrom |
WO2003050251A2 (en) | 2001-12-07 | 2003-06-19 | Geron Corporation | Hematopoietic cells from human embryonic stem cells |
CN101240262A (en) | 2001-12-07 | 2008-08-13 | 杰龙公司 | Islet cells from human embryonic stem cells |
AU2002366602B2 (en) | 2001-12-07 | 2008-07-03 | Asterias Biotherapeutics, Inc. | Chondrocyte precursors derived from human embryonic stem cells |
AU2002357410A1 (en) | 2001-12-21 | 2003-07-09 | Thromb-X Nv | Compositions for the in vitro derivation and culture of embryonic stem (es) cell lines with germline transmission capability and for the culture of adult stem cells |
US20050095703A1 (en) | 2001-12-28 | 2005-05-05 | Henrik Semb | Method for the establishment of a pluripotent human blastocyst - derived stem cell line |
AU2009213101B2 (en) | 2002-06-05 | 2012-03-08 | Es Cell International Pte Ltd | Generation of neural stem cells from undifferentiated human embryonic stem cells |
WO2003104423A2 (en) | 2002-06-11 | 2003-12-18 | Roy Ogle | Meningeal-derived stem cells |
US7662625B2 (en) | 2002-07-02 | 2010-02-16 | Cancer Research Technology Limited | Methods for detecting the differentiation status of cells using 5T4 antigen expression |
US7285415B2 (en) | 2002-07-11 | 2007-10-23 | The Regents Of The University Of California | Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury |
US20050101014A1 (en) | 2002-07-11 | 2005-05-12 | Keirstead Hans S. | Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury |
US7267981B2 (en) | 2002-10-07 | 2007-09-11 | Technion Research & Development Foundation Ltd. | Human foreskin fibroblasts for culturing ES cells |
US20060252150A1 (en) | 2002-11-08 | 2006-11-09 | Linzhao Cheng | Human embryonic stem cell cultures, and compositions and methods for growing same |
US20040110286A1 (en) | 2002-12-06 | 2004-06-10 | The John P. Robarts Research Institute | Method for making hematopoietic cells |
SI1572984T1 (en) | 2002-12-16 | 2016-07-29 | Technion Research & Development Foundation Ltd. | Feeder-free, xeno-free culture system for human embryonic stem cells |
WO2004078944A2 (en) | 2003-03-05 | 2004-09-16 | Maine Medical Center Research Institute | Thrombin, soluble jaggedi, and trap and growth of stem cells |
US20060134636A1 (en) | 2003-03-13 | 2006-06-22 | Stanton Lawrence W | Standardization of growth conditions for human embryonic stem cells intended for use in regenerative medicine |
US7153650B2 (en) | 2003-03-13 | 2006-12-26 | Geron Corporation | Marker system for preparing and characterizing high-quality human embryonic stem cells |
US20030224411A1 (en) | 2003-03-13 | 2003-12-04 | Stanton Lawrence W. | Genes that are up- or down-regulated during differentiation of human embryonic stem cells |
WO2004085631A2 (en) | 2003-03-28 | 2004-10-07 | Mount Sinai Hospital | Canine embryonic stem cells |
US7638328B2 (en) | 2003-05-08 | 2009-12-29 | Cellartis Ab | Method for efficient transfer of human blastocyst-derived stem cells (hBS cells) from a feeder-supported to a feeder-free culture system, long-term propagation of hBS cells under feeder-free conditions and use of cultured hBS cells for applications in myocardial regeneration |
US20040229350A1 (en) | 2003-05-12 | 2004-11-18 | Nikolai Strelchenko | Morula derived embryonic stem cells |
US20050118713A1 (en) | 2003-05-12 | 2005-06-02 | Nikolai Strelchenko | Morula derived embryonic stem cells |
US7569385B2 (en) | 2003-08-14 | 2009-08-04 | The Regents Of The University Of California | Multipotent amniotic fetal stem cells |
CA2549158A1 (en) | 2003-12-01 | 2005-06-16 | Technion Research And Development Foundation Ltd. | Methods of generating stem cells and embryonic bodies carrying disease-causing mutations and methods of using same for studying genetic disorders |
TWI280280B (en) | 2003-12-09 | 2007-05-01 | Ind Tech Res Inst | Culture system and method for expansion and undifferentiated growth of human embryonic stem cells |
GB2441530B (en) | 2004-02-12 | 2009-09-23 | Univ Newcastle | Stem Cells |
US20060216821A1 (en) | 2004-02-26 | 2006-09-28 | Reliance Life Sciences Pvt. Ltd. | Pluripotent embryonic-like stem cells derived from corneal limbus, methods of isolation and uses thereof |
WO2005086845A2 (en) | 2004-03-10 | 2005-09-22 | Regents Of The University Of California | Compositions and methods for growth of embryonic stem cells |
IL178006A (en) | 2004-03-19 | 2011-08-31 | Geron Corp | Method for making high purity cardiomyocyte preparations suitable for regenerative medicine |
CN1969040B (en) | 2004-03-19 | 2014-12-10 | 阿斯特利亚斯生物治疗股份公司 | Method for making high purity cardiomyocyte preparations suitable for regenerative medicine |
RU2375448C2 (en) | 2004-03-23 | 2009-12-10 | Асубио Фарма Ко., Лтд. | Method of cultivation pluripotential stem cells |
US7452718B2 (en) | 2004-03-26 | 2008-11-18 | Geron Corporation | Direct differentiation method for making cardiomyocytes from human embryonic stem cells |
US20050214938A1 (en) | 2004-03-26 | 2005-09-29 | Gold Joseph D | Cardiac bodies: clusters of spontaneously contracting cells for regenerating cardiac function |
US20050227352A1 (en) | 2004-04-09 | 2005-10-13 | Stowers Institute For Medical Research | Use of BMP (BAM) signaling to control stem cell fate determination |
WO2005113747A2 (en) | 2004-05-21 | 2005-12-01 | Fraunhofer Gesellschaft zur Förderung der angewandten Forschung e.V. | Multicellular tissue and organ culture systems |
EP1749091B1 (en) | 2004-05-21 | 2013-07-31 | Wicell Research Institute, Inc. | Feeder independent extended culture of embryonic stem cells |
US20080038820A1 (en) | 2004-06-22 | 2008-02-14 | Rudy-Reil Diane E | Induction of pluripotent stem cells into mesodermal lineages |
US20090136559A1 (en) | 2004-07-09 | 2009-05-28 | Athanasiou Kyriacos A | Chondrocyte Differentiation from Human Embryonic Stem Cells and Their Use in Tissue Engineering |
AU2005271723B2 (en) | 2004-07-13 | 2010-12-16 | Asterias Biotherapeutics, Inc. | Medium for growing human embryonic stem cells |
CA2504451A1 (en) | 2004-08-10 | 2006-02-10 | Geron Corporation | Dendritic cell vaccines for treating cancer made from embryonic stem cells |
US20080311607A1 (en) | 2004-08-12 | 2008-12-18 | Board Of Regents Of The University Of Texas System | Methods and Compositions for Regulation of Stem Cell Survival, Proliferation, and Differentiation by Protein Ubiquitination |
DE102004043256B4 (en) | 2004-09-07 | 2013-09-19 | Rheinische Friedrich-Wilhelms-Universität Bonn | Scalable process for culturing undifferentiated stem cells in suspension |
NZ553235A (en) | 2004-09-08 | 2009-11-27 | Wisconsin Alumni Res Found | Culturing human pluripotent stem cells |
CA2580754A1 (en) | 2004-09-21 | 2006-03-30 | Nvr Labs, Ltd. | Compositions and methods for stem cell expansion and differentiation |
WO2006040763A2 (en) | 2004-10-12 | 2006-04-20 | Technion Research & Development Foundation Ltd. | Isolated primate embryonic cells and methods of generating and using same |
JP4646600B2 (en) * | 2004-11-02 | 2011-03-09 | オリンパス株式会社 | Method for culturing mesenchymal stem cells |
US7641897B2 (en) | 2004-11-23 | 2010-01-05 | The Board Of Trustees Of The Leland Stanford Junior University | Feeder layer and serum independent embryonic stem cells |
FR2878860A1 (en) | 2004-12-08 | 2006-06-09 | Vivalis Sa | HUMAN STEM CELL LINES DERIVED FROM ES CELLS AND USES FOR THE PRODUCTION OF RECOMBINANT VACCINES AND PROTEINS |
US8597947B2 (en) | 2004-12-29 | 2013-12-03 | Hadasit Medical Research Services & Development Limited | Undifferentiated stem cell culture systems |
EP2410043A3 (en) | 2004-12-29 | 2013-01-23 | Hadasit Medical Research Services And Development Ltd. | Stem cells culture systems |
GB2437689B (en) | 2005-01-28 | 2009-10-28 | Novathera Ltd | Culture and screening methods |
WO2006088867A2 (en) | 2005-02-15 | 2006-08-24 | Medistem Laboratories, Incorporated | Method for expansion of stem cells |
RU2007135164A (en) | 2005-02-23 | 2009-03-27 | Эйдженси Фор Сайенс, Текнолоджи Энд Рисерч (Sg) | IMMORTALIZED FEEDER CELLS |
US8012751B2 (en) | 2005-03-31 | 2011-09-06 | Wisconsin Alumni Research Foundation | Differentiation of pluripotent embryonic stem cells |
US7972767B2 (en) | 2005-05-09 | 2011-07-05 | Olympus Corporation | Method for culturing mesenchymal stem cell and method for producing biological tissue prosthesis |
JP4755460B2 (en) * | 2005-07-27 | 2011-08-24 | オリンパス株式会社 | Method for culturing mesenchymal stem cells |
US9446073B2 (en) | 2005-05-15 | 2016-09-20 | Biodontos, Llc | Non-lineage committed precursor cells from the dental papillary tissue of teeth |
BRPI0612423A2 (en) | 2005-05-17 | 2009-01-27 | Reliance Life Sciences Pvt Ltd | pedigree establishment of human embryonic stem cells using mammalian cells |
US20110311977A1 (en) | 2005-05-17 | 2011-12-22 | Arundhati Mandal | In Vitro Generation of Hepatocytes from Human Embryonic Stem Cells |
WO2007002210A2 (en) | 2005-06-20 | 2007-01-04 | Bresagen, Inc. | Embryonic stem cell culture compositions and methods of use thereof |
WO2007002086A2 (en) | 2005-06-22 | 2007-01-04 | Geron Corporation | Suspension culture of human embryonic stem cells |
CA2615396A1 (en) * | 2005-07-15 | 2007-01-25 | Primegen Biotech, Llc | Therapeutic reprogramming of germ line stem cells |
EP1962719A4 (en) | 2005-08-29 | 2011-05-04 | Technion Res And Dev Of Foundation Ltd | Media for culturing stem cells |
US7754483B2 (en) | 2005-11-09 | 2010-07-13 | The Penn State Research Foundation | Systems and methods for selection and maintenance of homogeneous and pluripotent human embryonic stem cells |
US20080070303A1 (en) | 2005-11-21 | 2008-03-20 | West Michael D | Methods to accelerate the isolation of novel cell strains from pluripotent stem cells and cells obtained thereby |
EA018039B1 (en) | 2005-12-13 | 2013-05-30 | Киото Юниверсити | Nuclear reprogramming factor |
US20070249044A1 (en) | 2006-01-30 | 2007-10-25 | University Of Illinois At Chicago | Microstructures in three dimensional gel suspensions for growth of cells |
US9115342B2 (en) | 2006-01-31 | 2015-08-25 | Daiichi Sankyo Company | Method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses |
SG10201405107YA (en) | 2006-02-23 | 2014-10-30 | Viacyte Inc | Compositions and methods useful for culturing differentiable cells |
JP2007228815A (en) | 2006-02-27 | 2007-09-13 | Gifu Univ | Method for maintaining embryonic stem cell |
WO2007122233A1 (en) | 2006-04-25 | 2007-11-01 | Vrije Universiteit Brussel | Preparation of mesenchymal progenitor cells, particularly osteogenic progenitor cells |
US8241908B2 (en) | 2006-05-16 | 2012-08-14 | Becton, Dickinson And Company | Extracellular matrix coated surface for culturing cells |
US9096831B2 (en) | 2006-05-19 | 2015-08-04 | Versitech Limited | Methods to enhance cell migration and engraftment |
GB0613756D0 (en) * | 2006-07-12 | 2006-08-23 | Univ Sheffield | Cell culture medium |
US20100069251A1 (en) | 2006-09-15 | 2010-03-18 | Children's Medical Center Corporation | Methods for producing embryonic stem cells from parthenogenetic embryos |
WO2008054819A2 (en) | 2006-11-02 | 2008-05-08 | The General Hospital Corporation | Cardiovascular stem cells, methods for stem cell isolation, and uses thereof |
WO2008058942A2 (en) | 2006-11-13 | 2008-05-22 | Charite - Universitätsmedezin Berlin | Method of cell culture and method of treatment comprising a vepo protein variant |
US8414928B2 (en) | 2006-12-06 | 2013-04-09 | George R. Dodge | Methods for the generation of cartilage-like material by mechanical loading |
WO2008075378A2 (en) | 2006-12-19 | 2008-06-26 | Stempeutics Research Private Limited | Xeno-free culture conditions for human embryonic stem cells and methods thereof |
US20080182328A1 (en) | 2006-12-19 | 2008-07-31 | The Burnham Institute | Mammalian extraembryonic endoderm cells and methods of isolation |
CN101605885B (en) | 2007-01-04 | 2013-03-27 | 卡尔·特吕格瓦松 | Composition and method for enabling proliferation of pluripotent stem cells |
US10829733B2 (en) | 2007-01-04 | 2020-11-10 | Biolamina Ab | Composition and method for enabling proliferation of pluripotent human stem cells |
GB2458863B (en) | 2007-01-17 | 2011-10-12 | Wisconsin Alumni Res Found | Improved culture of stem cells |
WO2008087257A1 (en) | 2007-01-18 | 2008-07-24 | Suomen Punainen Risti, Veripalvelu | Novel methods and reagents directed to production of cells |
US20110033928A1 (en) | 2007-03-09 | 2011-02-10 | The Regents Of The University Of Michigan | Methods and compositions for growth of cells and embryonic tissue on a synthetic polymer matrix |
DE102007020302B4 (en) | 2007-04-20 | 2012-03-22 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Improved three-dimensional biocompatible scaffold structure that incorporates nanoparticles |
WO2008148105A1 (en) | 2007-05-25 | 2008-12-04 | Medistem Laboratories, Inc. | Endometrial stem cells and methods of making and using same |
FI20075417A0 (en) * | 2007-06-05 | 2007-06-05 | Marjo-Riitta Suuronen | Formulations and methods for culturing embryonic stem cells |
DK2173863T3 (en) | 2007-06-29 | 2019-01-21 | Fujifilm Cellular Dynamics Inc | Automated method and apparatus for embryonic stem cell culture |
EP2028268A1 (en) | 2007-08-20 | 2009-02-25 | Université Libre De Bruxelles | Generation of neuronal cells from pluripotent stem cells |
EP2191022A2 (en) | 2007-08-21 | 2010-06-02 | The Salk Institute for Biological Studies | Robust regression based exon array protocol system and applications |
US8623650B2 (en) | 2007-10-19 | 2014-01-07 | Viacyte, Inc. | Methods and compositions for feeder-free pluripotent stem cell media containing human serum |
EP2072618A1 (en) | 2007-12-14 | 2009-06-24 | Johannes Gutenberg-Universität Mainz | Use of RNA for reprogramming somatic cells |
CA2709566A1 (en) | 2007-12-17 | 2009-06-25 | Gliamed, Inc. | Stem-like cells and method for reprogramming adult mammalian somatic cells |
JP2011510658A (en) * | 2008-01-30 | 2011-04-07 | ジェロン・コーポレーション | Synthetic surface for culturing stem cell-derived cardiomyocytes |
BRPI0906724A2 (en) | 2008-01-30 | 2019-05-07 | Geron Corp | cell culture article and screening |
CA2725251A1 (en) | 2008-05-27 | 2009-12-17 | Aarhus Universitet | Biocompatible materials for mammalian stem cell growth and differentiation |
US20100120142A1 (en) | 2008-07-11 | 2010-05-13 | Suomen Punainen Risti Veripalvelu | Culture of human embryonic cells |
US20100035327A1 (en) | 2008-08-11 | 2010-02-11 | Ann Marie Steele | Use of rice-derived products in a universal cell culture medium |
SG160248A1 (en) | 2008-09-18 | 2010-04-29 | Agency Science Tech & Res | Use of novel monoclonal antibodies targeting human embryonic stem cells to characterize and kill induced pluripotent stem cells |
US8895300B2 (en) | 2008-11-04 | 2014-11-25 | Viacyte, Inc. | Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof |
US8008075B2 (en) | 2008-11-04 | 2011-08-30 | Viacyte, Inc. | Stem cell aggregate suspension compositions and methods of differentiation thereof |
US8956867B2 (en) | 2008-11-07 | 2015-02-17 | Wisconsin Alumni Research Foundation | Method for culturing stem cells |
KR101720961B1 (en) | 2009-02-27 | 2017-03-29 | 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 | Differentiation of pluripotent cells |
CN102741396B (en) | 2009-04-27 | 2017-10-20 | 威盛细胞有限公司 | Support the small molecule and its method of pluripotent cell growth |
US20120142103A1 (en) | 2009-05-18 | 2012-06-07 | Kohji Nishida | Method for inducing differentiation into epithelial progenitor cell/stem cell population and corneal epithelial cell population from induced pluripotent stem cells |
JP2012529890A (en) | 2009-06-11 | 2012-11-29 | ミネルバ バイオテクノロジーズ コーポレーション | Method for culturing stem cells and progenitor cells |
TW201111328A (en) | 2009-06-12 | 2011-04-01 | Nanyang Polytechnic | Novel uses |
KR101166257B1 (en) | 2009-06-16 | 2012-07-19 | 한국생명공학연구원 | Media composition comprising neuropeptide y for the generation, maintenance, prologned undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same |
WO2011019953A1 (en) | 2009-08-12 | 2011-02-17 | University Of Southern California | Defined conditions for human embryonic stem cell culture and passage |
US8415155B2 (en) | 2009-10-19 | 2013-04-09 | Cellular Dynamics International, Inc. | Cardiomyocyte production |
CA3080762A1 (en) | 2009-11-12 | 2011-05-19 | Technion Research & Development Foundation Limited | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
KR20120102709A (en) | 2009-11-17 | 2012-09-18 | 어드밴스드 셀 테크놀로지, 인코포레이티드 | Methods of producing human rpe cells and pharmaceutical preparations of human rpe cells |
EP2512516B1 (en) | 2009-12-18 | 2016-02-17 | The Governing Council Of The University Of Toronto | Injectable polymer composition for use as a cell delivery vehicle |
US8609416B2 (en) | 2009-12-18 | 2013-12-17 | Ventria Bioscience | Methods and compositions comprising heat shock proteins |
US20140030236A1 (en) | 2010-02-25 | 2014-01-30 | The Johns Hopkins University | Derivation and maturation of synthetic and contractile vascular smooth muscle cells from human pluripotent stem cells |
WO2011110886A1 (en) | 2010-03-09 | 2011-09-15 | Biolamina Ab | Composition and method for enabling proliferation of pluripotent human stem cells |
EP2561087A4 (en) | 2010-04-20 | 2013-10-16 | Int Stem Cell Corp | Physiological methods for isolation of high purity cell populations |
WO2011140397A2 (en) | 2010-05-05 | 2011-11-10 | The Regents Of The University Of California Office Of The President | Stem cell defined media for xeno-free and feeder free conditions and uses thereof |
US11786636B2 (en) | 2010-06-15 | 2023-10-17 | Versitech Limited | Methods for complex tissue engineering |
WO2011160128A2 (en) | 2010-06-18 | 2011-12-22 | Cellular Dynamics International, Inc. | Cardiomyocyte medium with dialyzed serum |
PL2596119T3 (en) | 2010-07-23 | 2021-12-06 | Astellas Institute For Regenerative Medicine | Methods for detection of rare subpopulations of cells and highly purified compositions of cells |
US20120141432A1 (en) | 2010-09-03 | 2012-06-07 | University Of Pittsburgh- Of The Commonwealth System Of Higher Education | Use of catalytic antioxidant to preserve stem cell phenotype and control cell differentiation |
DK2614141T3 (en) | 2010-09-07 | 2019-09-09 | Technion Res & Dev Foundation | PRESENT PROCEDURES AND CULTIVATION MEDIA FOR CULTURING PLURIPOTENT STEM CELLS |
WO2012034101A2 (en) | 2010-09-09 | 2012-03-15 | The Regents Of The University Of California | Expandable cell source of neuronal stem cell populations and methods for obtaining and using them |
-
2010
- 2010-11-11 CA CA3080762A patent/CA3080762A1/en active Pending
- 2010-11-11 ES ES10829622T patent/ES2779048T3/en active Active
- 2010-11-11 CA CA2783437A patent/CA2783437C/en active Active
- 2010-11-11 ES ES19210132T patent/ES2932664T3/en active Active
- 2010-11-11 JP JP2012538468A patent/JP6276918B2/en active Active
- 2010-11-11 EP EP22185625.5A patent/EP4166652A1/en active Pending
- 2010-11-11 EP EP10829622.9A patent/EP2499236B1/en active Active
- 2010-11-11 US US13/508,991 patent/US9018010B2/en active Active
- 2010-11-11 WO PCT/IL2010/000937 patent/WO2011058558A2/en active Application Filing
- 2010-11-11 CA CA3174901A patent/CA3174901A1/en active Pending
- 2010-11-11 EP EP19210132.7A patent/EP3633025B1/en active Active
- 2010-11-11 DK DK10829622.9T patent/DK2499236T3/en active
- 2010-11-11 DK DK19210132.7T patent/DK3633025T3/en active
-
2015
- 2015-04-27 US US14/696,520 patent/US10876094B2/en active Active
- 2015-10-22 JP JP2015207773A patent/JP2016047056A/en active Pending
-
2017
- 2017-06-01 IL IL252607A patent/IL252607B/en active IP Right Grant
- 2017-08-07 JP JP2017152419A patent/JP6603694B2/en active Active
-
2019
- 2019-10-11 JP JP2019188087A patent/JP7053551B2/en active Active
-
2020
- 2020-11-13 US US17/097,023 patent/US20210079342A1/en active Pending
-
2022
- 2022-03-31 JP JP2022059325A patent/JP7383069B2/en active Active
- 2022-06-16 US US17/841,700 patent/US20220333066A1/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050233446A1 (en) * | 2003-12-31 | 2005-10-20 | Parsons Xuejun H | Defined media for stem cell culture |
WO2008015682A2 (en) * | 2006-08-02 | 2008-02-07 | Technion Research & Development Foundation Ltd. | Methods of expanding embryonic stem cells in a suspension culture |
US20090191159A1 (en) * | 2007-06-15 | 2009-07-30 | Kazuhiro Sakurada | Multipotent/pluripotent cells and methods |
Also Published As
Publication number | Publication date |
---|---|
JP2020022480A (en) | 2020-02-13 |
CA2783437A1 (en) | 2011-05-19 |
JP2013510567A (en) | 2013-03-28 |
IL252607B (en) | 2019-02-28 |
CA3080762A1 (en) | 2011-05-19 |
DK3633025T3 (en) | 2022-12-12 |
EP3633025A1 (en) | 2020-04-08 |
CA3174901A1 (en) | 2011-05-19 |
CA2783437C (en) | 2020-08-04 |
WO2011058558A2 (en) | 2011-05-19 |
US20150240202A1 (en) | 2015-08-27 |
ES2932664T3 (en) | 2023-01-23 |
US10876094B2 (en) | 2020-12-29 |
JP6276918B2 (en) | 2018-02-07 |
EP4166652A1 (en) | 2023-04-19 |
JP6603694B2 (en) | 2019-11-06 |
JP2017225456A (en) | 2017-12-28 |
IL252607A0 (en) | 2017-07-31 |
EP2499236A2 (en) | 2012-09-19 |
EP2499236A4 (en) | 2013-05-22 |
JP2022088599A (en) | 2022-06-14 |
US9018010B2 (en) | 2015-04-28 |
EP2499236B1 (en) | 2020-01-01 |
JP7383069B2 (en) | 2023-11-17 |
US20120225480A1 (en) | 2012-09-06 |
JP2016047056A (en) | 2016-04-07 |
JP7053551B2 (en) | 2022-04-12 |
DK2499236T3 (en) | 2020-03-23 |
EP3633025B1 (en) | 2022-09-14 |
ES2779048T3 (en) | 2020-08-13 |
US20210079342A1 (en) | 2021-03-18 |
US20220333066A1 (en) | 2022-10-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2011058558A3 (en) | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state | |
WO2011159359A3 (en) | Serum-free chemically defined cell culture medium | |
AU2019268194A1 (en) | Cell culture system | |
JP2013510567A5 (en) | ||
SG150567A1 (en) | Pluripotent stem cell growing method | |
WO2012032521A3 (en) | Novel methods and culture media for culturing pluripotent stem cells | |
WO2008004990A3 (en) | Method for stem cell culture and cells derived therefrom | |
MX343462B (en) | Cell culture medium. | |
WO2008129563A3 (en) | Human mesenchymal stem cells and preparation thereof | |
WO2007115337A3 (en) | Chondrocyte differentiation from human embryonic stem cells and their use in tissue engineering | |
WO2011005773A3 (en) | Method of culturing eukaryotic cells | |
WO2010147395A3 (en) | Medium composition comprising neuropeptide y for the generation, maintenance, prologned undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same | |
CA2863795A1 (en) | Culturing of mesenchymal stem cells | |
EP4223769A3 (en) | Nuclear reprogramming factor | |
WO2004055155A3 (en) | Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and stem cell cultures prepared using same | |
EP1789534A4 (en) | Isolation of stem/progenitor cells from amniotic membrane of umbilical cord. | |
RU2011124900A (en) | CULTURE OF PLURIPOTENT STEM CELLS ON MICRO-CARRIERS | |
WO2007088372A3 (en) | Cell culture | |
WO2006070370A3 (en) | Stem cells culture systems | |
RU2016121404A (en) | SUSPENDING AND CLUSTERING OF HUMAN PLURIPOTENT STEM CELLS WITH THE PURPOSE OF THEIR DIFFERENTIATION IN PANCREATIC ENDOCRINE CELLS | |
WO2008120218A3 (en) | Undifferentiated stem cell culture systems | |
WO2012034101A3 (en) | Expandable cell source of neuronal stem cell populations and methods for obtaining and using them | |
WO2008152640A3 (en) | Three dimensional biocompatible scaffolds for ex-vivo expansion and transplantation of stem cells | |
WO2005094353A3 (en) | Methods for production of regulatory t cells and uses thereof | |
SG179458A1 (en) | Growth medium for c. histolyticum without ingredients of mamalian sources |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 10829622 Country of ref document: EP Kind code of ref document: A1 |
|
ENP | Entry into the national phase |
Ref document number: 2783437 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 219736 Country of ref document: IL Ref document number: 13508991 Country of ref document: US |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2012538468 Country of ref document: JP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2010829622 Country of ref document: EP |