WO2011132938A3 - Gpcr-bpb specifically binding to gpcr - Google Patents

Gpcr-bpb specifically binding to gpcr Download PDF

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Publication number
WO2011132938A3
WO2011132938A3 PCT/KR2011/002838 KR2011002838W WO2011132938A3 WO 2011132938 A3 WO2011132938 A3 WO 2011132938A3 KR 2011002838 W KR2011002838 W KR 2011002838W WO 2011132938 A3 WO2011132938 A3 WO 2011132938A3
Authority
WO
WIPO (PCT)
Prior art keywords
gpcr
present
peptide binder
bipodal peptide
region
Prior art date
Application number
PCT/KR2011/002838
Other languages
French (fr)
Korean (ko)
Other versions
WO2011132938A2 (en
Inventor
전상용
김성현
박세호
김대진
이상헌
Original Assignee
광주과학기술원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 광주과학기술원 filed Critical 광주과학기술원
Priority to KR1020127023551A priority Critical patent/KR20130103300A/en
Publication of WO2011132938A2 publication Critical patent/WO2011132938A2/en
Publication of WO2011132938A3 publication Critical patent/WO2011132938A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/0002—General or multifunctional contrast agents, e.g. chelated agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/005—Fluorescence in vivo characterised by the carrier molecule carrying the fluorescent agent
    • A61K49/0056—Peptides, proteins, polyamino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH

Abstract

The present invention relates to a GPCR-bipodal peptide binder specifically binding to GPCR, comprising: (a) a structure stabilizing region comprising parallel, antiparallel, or parallel and antiparallel amino acid strands in which an interstrand noncovalent bond is formed; and (b) a GPCR-target binding region I and a GPCR-target binding region II which bind respectively to both ends of the structure stabilizing region and comprise randomly selected n and m amino acids, respectively. The GPCR-bipodal peptide binder of the present invention shows a very low level (for example, nM level) of KD value (dissociation constant) to GPCR, thereby showing very high affinity to a GPCR target. The GPCR-bipodal peptide binder of the present invention has medical use, can be used for in vivo molecular imaging, in vitro cell imaging and targeting for drug delivery, and also can be very usefully used as an escort molecule.
PCT/KR2011/002838 2010-04-20 2011-04-20 Gpcr-bpb specifically binding to gpcr WO2011132938A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020127023551A KR20130103300A (en) 2010-04-20 2011-04-20 Gprr-bpb specifically binding to gpcr

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2010-0036599 2010-04-20
KR20100036599 2010-04-20

Publications (2)

Publication Number Publication Date
WO2011132938A2 WO2011132938A2 (en) 2011-10-27
WO2011132938A3 true WO2011132938A3 (en) 2012-05-10

Family

ID=44834644

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/002838 WO2011132938A2 (en) 2010-04-20 2011-04-20 Gpcr-bpb specifically binding to gpcr

Country Status (2)

Country Link
KR (1) KR20130103300A (en)
WO (1) WO2011132938A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101531944B1 (en) * 2013-12-24 2015-06-29 광주과학기술원 VEGF-BPB specifically binding to VEGF

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5427908A (en) * 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
US6914123B2 (en) * 2001-04-17 2005-07-05 Genentech, Inc. Hairpin peptides with a novel structural motif and methods relating thereto
KR20100044128A (en) * 2008-10-20 2010-04-29 광주과학기술원 Bipodal peptide binder

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5427908A (en) * 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
US6914123B2 (en) * 2001-04-17 2005-07-05 Genentech, Inc. Hairpin peptides with a novel structural motif and methods relating thereto
KR20100044128A (en) * 2008-10-20 2010-04-29 광주과학기술원 Bipodal peptide binder

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
2010 KOREAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY ANNUAL INTERNATIONAL CONFERENCE POSTER SESSTION A-18-85, 17 May 2010 (2010-05-17), pages 153 *
BAGALKOT, V. ET AL.: "An aptamer doxorubicin physical conjugate as a novel targeted drug-delivery platform.", ANGEW. CHEM. INT. ED., vol. 45, 2006, pages 8149 - 8152 *
CLACKSON, T. ET AL.: "Making antibody fragments using phage display libraries.", NATURE, vol. 352, 15 August 1991 (1991-08-15), pages 624 - 628 *

Also Published As

Publication number Publication date
KR20130103300A (en) 2013-09-23
WO2011132938A2 (en) 2011-10-27

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