WO2011132940A3 - Rtk-bpb specifically binding to rtk - Google Patents

Rtk-bpb specifically binding to rtk Download PDF

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Publication number
WO2011132940A3
WO2011132940A3 PCT/KR2011/002840 KR2011002840W WO2011132940A3 WO 2011132940 A3 WO2011132940 A3 WO 2011132940A3 KR 2011002840 W KR2011002840 W KR 2011002840W WO 2011132940 A3 WO2011132940 A3 WO 2011132940A3
Authority
WO
WIPO (PCT)
Prior art keywords
cytokine
rtk
present
peptide binder
bipodal peptide
Prior art date
Application number
PCT/KR2011/002840
Other languages
French (fr)
Korean (ko)
Other versions
WO2011132940A2 (en
Inventor
전상용
김성현
박세호
김대진
이상헌
Original Assignee
광주과학기술원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 광주과학기술원 filed Critical 광주과학기술원
Priority to KR1020127023550A priority Critical patent/KR20130103299A/en
Publication of WO2011132940A2 publication Critical patent/WO2011132940A2/en
Publication of WO2011132940A3 publication Critical patent/WO2011132940A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/715—Assays involving receptors, cell surface antigens or cell surface determinants for cytokines; for lymphokines; for interferons

Abstract

The present invention relates to a cytokine-bipodal peptide binder specifically binding to cytokine, comprising (a) a structure stabilizing region comprising parallel, antiparallel, or parallel and antiparallel amino acid strands in which an interstrand noncovalent bond is formed; and (b) a cytokine-target binding region I and a cytokine-target binding region II which bind to both ends of the structure stabilizing region and comprise n and m amino acids randomly selected, respectively. The cytokine-bipodal peptide binder of the present invention shows a very low level (for example, nM level) of KD value (dissociation constant) to cytokine, thereby showing very high affinity to a cytokine target. The cytokine-bipodal peptide binder of the present invention has medical use, can be used for in vivo molecular imaging and targeting for drug delivery, and is very useful as an escort molecule.
PCT/KR2011/002840 2010-04-20 2011-04-20 Rtk-bpb specifically binding to rtk WO2011132940A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020127023550A KR20130103299A (en) 2010-04-20 2011-04-20 Rtk-bpb specifically binding to rtk

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2010-0036608 2010-04-20
KR20100036608 2010-04-20

Publications (2)

Publication Number Publication Date
WO2011132940A2 WO2011132940A2 (en) 2011-10-27
WO2011132940A3 true WO2011132940A3 (en) 2012-04-05

Family

ID=44834646

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/002840 WO2011132940A2 (en) 2010-04-20 2011-04-20 Rtk-bpb specifically binding to rtk

Country Status (2)

Country Link
KR (1) KR20130103299A (en)
WO (1) WO2011132940A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2909226A4 (en) * 2012-10-19 2016-04-13 Nat Res Council Canada Derivatives of collagen-binding hairpin peptides

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5427908A (en) * 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
JP2001002592A (en) * 1999-06-18 2001-01-09 Dai Ichi Seiyaku Co Ltd Composition for gene-transducing
US20040152647A1 (en) * 2003-01-31 2004-08-05 Max-Delbruck-Cenrum Fur Molekulare Medizin Agent for gene transfer
US6914123B2 (en) * 2001-04-17 2005-07-05 Genentech, Inc. Hairpin peptides with a novel structural motif and methods relating thereto
WO2010047515A2 (en) * 2008-10-20 2010-04-29 광주과학기술원 Bipodal peptide binder

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5427908A (en) * 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
JP2001002592A (en) * 1999-06-18 2001-01-09 Dai Ichi Seiyaku Co Ltd Composition for gene-transducing
US6914123B2 (en) * 2001-04-17 2005-07-05 Genentech, Inc. Hairpin peptides with a novel structural motif and methods relating thereto
US20040152647A1 (en) * 2003-01-31 2004-08-05 Max-Delbruck-Cenrum Fur Molekulare Medizin Agent for gene transfer
WO2010047515A2 (en) * 2008-10-20 2010-04-29 광주과학기술원 Bipodal peptide binder

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
TIM CLACKSON ET AL.: "Making antibody fragments using phage display libraries", NATURE, vol. 352, 15 August 1991 (1991-08-15), pages 624 - 628 *
VAISHALI BAGALKOT E ET AL.: "An Aptamer-Doxorubicin Physical Conjugate as a Novel Targeted Drug-Delivery Platform", ANGEW. CHEM. INT. ED., vol. 45, 2006, pages 8149 - 8152 *

Also Published As

Publication number Publication date
WO2011132940A2 (en) 2011-10-27
KR20130103299A (en) 2013-09-23

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