WO2011162826A3 - Group culture system and method with helper embryos - Google Patents

Group culture system and method with helper embryos Download PDF

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Publication number
WO2011162826A3
WO2011162826A3 PCT/US2011/001135 US2011001135W WO2011162826A3 WO 2011162826 A3 WO2011162826 A3 WO 2011162826A3 US 2011001135 W US2011001135 W US 2011001135W WO 2011162826 A3 WO2011162826 A3 WO 2011162826A3
Authority
WO
WIPO (PCT)
Prior art keywords
embryos
group
culture system
group culture
helper
Prior art date
Application number
PCT/US2011/001135
Other languages
French (fr)
Other versions
WO2011162826A2 (en
Inventor
Fuliang Du
Giorgio Antonio Presicce
Juan Moreno
Jie Xu
Shinn-Chin Wu
Original Assignee
Inguran, Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inguran, Llc filed Critical Inguran, Llc
Priority to CA2803814A priority Critical patent/CA2803814A1/en
Priority to BR112012032921A priority patent/BR112012032921A2/en
Publication of WO2011162826A2 publication Critical patent/WO2011162826A2/en
Publication of WO2011162826A3 publication Critical patent/WO2011162826A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0012—Cell encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0604—Whole embryos; Culture medium therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/02—Coculture with; Conditioned medium produced by embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/76—Agarose, agar-agar

Abstract

Methods and systems for physically separating helper embryos from desired embryos in a group culturing technique in order to maintain the pedigree and genetic information of the desired embryos from different species, including cattle and human, and to provide the developmental benefits of group culturing. The separation of the groups of embryos can be the result of embedding one group of embryos in a gel or solid, or the groups can be physically separated by a membrane or other structure.
PCT/US2011/001135 2010-06-23 2011-06-24 Group culture system and method with helper embryos WO2011162826A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA2803814A CA2803814A1 (en) 2010-06-23 2011-06-24 Group culture system and method with helper embryos
BR112012032921A BR112012032921A2 (en) 2010-06-23 2011-06-24 group cultivation system and method with helper embryos

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35786910P 2010-06-23 2010-06-23
US61/357,869 2010-06-23

Publications (2)

Publication Number Publication Date
WO2011162826A2 WO2011162826A2 (en) 2011-12-29
WO2011162826A3 true WO2011162826A3 (en) 2014-04-10

Family

ID=45372004

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/001135 WO2011162826A2 (en) 2010-06-23 2011-06-24 Group culture system and method with helper embryos

Country Status (3)

Country Link
BR (1) BR112012032921A2 (en)
CA (1) CA2803814A1 (en)
WO (1) WO2011162826A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102559585A (en) * 2012-02-03 2012-07-11 兰诺生物技术无锡有限公司 Culture medium for in vitro culture of bovine embryo

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080145860A1 (en) * 2001-04-13 2008-06-19 Lee Ike W Encapsulated cell indicator system
US20100036492A1 (en) * 2008-07-06 2010-02-11 The Curators Of The University Of Missouri Osteochondral implants, arthroplasty methods, devices, and systems

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080145860A1 (en) * 2001-04-13 2008-06-19 Lee Ike W Encapsulated cell indicator system
US20100036492A1 (en) * 2008-07-06 2010-02-11 The Curators Of The University Of Missouri Osteochondral implants, arthroplasty methods, devices, and systems

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GOPICHANDRAN ET AL.: "The effect of paracrine/autocrine interactions on the in vitro culture of bovine preimplantation embryos.", REPRODUCTION, vol. 131, 2006, pages 269 - 277 *
SHEN ET AL.: "Endothelial cells stimulate self-renewal and expand neurogenesis of neural stem cells.", SCIENCE, vol. 304, 2004, pages 1338 - 1340 *
WILLADSEN ET AL.: "The viability of early cleavage stages containing half the normal number of blastomeres in the sheep.", J REPROD FERT, vol. 59, 1980, pages 357 - 362 *

Also Published As

Publication number Publication date
BR112012032921A2 (en) 2015-10-06
CA2803814A1 (en) 2011-12-29
WO2011162826A2 (en) 2011-12-29

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