WO2012095078A3 - Arrangement for carrying out individual sample analysis and manipulation for raman microspectroscopy - Google Patents

Arrangement for carrying out individual sample analysis and manipulation for raman microspectroscopy Download PDF

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Publication number
WO2012095078A3
WO2012095078A3 PCT/DE2011/002150 DE2011002150W WO2012095078A3 WO 2012095078 A3 WO2012095078 A3 WO 2012095078A3 DE 2011002150 W DE2011002150 W DE 2011002150W WO 2012095078 A3 WO2012095078 A3 WO 2012095078A3
Authority
WO
WIPO (PCT)
Prior art keywords
fibres
laser
arrangement
collection
excitation
Prior art date
Application number
PCT/DE2011/002150
Other languages
German (de)
French (fr)
Other versions
WO2012095078A2 (en
Inventor
Jürgen Popp
Christoph Krafft
Sebastian Dochow
Original Assignee
Institut Für Phonetische Technologien E.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Für Phonetische Technologien E.V. filed Critical Institut Für Phonetische Technologien E.V.
Publication of WO2012095078A2 publication Critical patent/WO2012095078A2/en
Publication of WO2012095078A3 publication Critical patent/WO2012095078A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0346—Capillary cells; Microcells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N2021/651—Cuvettes therefore

Abstract

The invention relates to an arrangement for carrying out individual sample analysis and manipulation for Raman microspectroscopy and to a method for operating such an arrangement. The aim of the invention is to provide an arrangement for carrying out individual sample analysis and manipulation for Raman microspectroscopy, which requires no microscope and only one laser and enables lower power densities by comparison with the prior art. Said aim is achieved in that the arrangement comprises a planar substrate (1) into which a channel (11) for transporting samples and channels (12) containing optical fibres (4; 6) are introduced, wherein the channel (11) has an inlet (111) connected to sample supply means (81) and an outlet (112) connected to sample discharge means (9), and a laser trap (5) into which the fibres (4; 6) are optically coupled is disposed in the channel (11) between the inlet (111) and the outlet (112). The optical fibres (4) are at least two excitation and trapping fibres (41) and the optical fibres (6) are a plurality of collection fibres (61) which converge centrally on the laser trap (5), wherein an excitation and trapping fibre (41) is formed from an individual fibre, excitation and collection laser light can be simultaneously coupled into the laser trap (5) via the excitation and collection fibres (41), scattered light can be coupled out of the laser trap (5) via the collection fibres (61), individual samples can be generated by the means (81), the individual samples can be sorted by the means (9), and the collection fibres (61) open into a Raman spectrometer (7) and the excitation and collection fibres (41) open into a laser (2) with a beam splitter (3), wherein unfocussed laser beams can be generated by the laser (2).
PCT/DE2011/002150 2011-01-14 2011-12-14 Arrangement for carrying out individual sample analysis and manipulation for raman microspectroscopy WO2012095078A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102011008788.5 2011-01-14
DE201110008788 DE102011008788B3 (en) 2011-01-14 2011-01-14 Arrangement for performing a single-sample analysis and manipulation for Raman microspectroscopy

Publications (2)

Publication Number Publication Date
WO2012095078A2 WO2012095078A2 (en) 2012-07-19
WO2012095078A3 true WO2012095078A3 (en) 2012-09-07

Family

ID=45935985

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2011/002150 WO2012095078A2 (en) 2011-01-14 2011-12-14 Arrangement for carrying out individual sample analysis and manipulation for raman microspectroscopy

Country Status (2)

Country Link
DE (1) DE102011008788B3 (en)
WO (1) WO2012095078A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017089427A1 (en) * 2015-11-23 2017-06-01 Celltool Gmbh Device and method for analyzing biological objects with raman spectroscopy
CN111454832B (en) * 2020-04-27 2023-12-15 深圳大学 Cell sorting system and method based on micro-flow control

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004008121A2 (en) * 2002-07-16 2004-01-22 East Carolina University Laser tweezers and raman spectroscopy systems and methods for studying microscopic particles

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002503331A (en) * 1995-12-05 2002-01-29 ガメラ バイオサイエンス コーポレイション Apparatus and method for using centripetal acceleration to drive liquid transfer in a microfluidic device engineering system with onboard information science
DE19830596B4 (en) * 1997-07-10 2008-08-21 Ruprecht-Karls-Universität Heidelberg Wave field microscope, wave field microscopy method, also for DNA sequencing, and calibration method for wave field microscopy
DE19742227A1 (en) * 1997-09-25 1999-04-01 Juergen Prof Dipl Phys Wolfrum Method of sequencing a single DNA molecule
US6852492B2 (en) * 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
GB0611289D0 (en) * 2006-06-08 2006-07-19 Univ St Andrews Raman spectroscopy

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004008121A2 (en) * 2002-07-16 2004-01-22 East Carolina University Laser tweezers and raman spectroscopy systems and methods for studying microscopic particles

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LAU ADRIAN Y ET AL: "An integrated optofluidic platform for Raman-activated cell sorting", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY, CAMBRIDGE, GB, vol. 8, 28 May 2008 (2008-05-28), pages 1116 - 1120, XP002519719, ISSN: 1473-0197, DOI: 10.1039/B803598A *
P. R. T. JESS ET AL: "Dual beam fibre trap for Raman micro-spectroscopy of single cells", OPTICS EXPRESS, vol. 14, no. 12, 1 January 2006 (2006-01-01), pages 5779, XP055030023, ISSN: 1094-4087, DOI: 10.1364/OE.14.005779 *

Also Published As

Publication number Publication date
WO2012095078A2 (en) 2012-07-19
DE102011008788B3 (en) 2012-05-03

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