WO2012131733A3 - Media compositions for enriching cultures of stem cells - Google Patents
Media compositions for enriching cultures of stem cells Download PDFInfo
- Publication number
- WO2012131733A3 WO2012131733A3 PCT/IN2012/000234 IN2012000234W WO2012131733A3 WO 2012131733 A3 WO2012131733 A3 WO 2012131733A3 IN 2012000234 W IN2012000234 W IN 2012000234W WO 2012131733 A3 WO2012131733 A3 WO 2012131733A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- present
- culture medium
- cells
- stem cells
- stem
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/33—Insulin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/90—Polysaccharides
- C12N2501/91—Heparin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
Abstract
The present invention provides a culture medium for selectively enriching and maintaining stem cells or cancer stem-like cells comprising basic nutrients, gelatin, methyl cellulose and optionally growth factors or other supplementary elements in an effective amount wherein the culture medium is essentially serum free. The present invention further provides feeding medium comprising components similar to the culture medium except the feeding medium is devoid of methyl cellulose. Present invention still further provides cell cultures comprising stem cells or cancer stem-like cells and the culture medium of the present invention. Also provides are methods for selectively enriching and maintaining stem cells or cancer stem-like cells using the culture medium of the present invention and kits for the same.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IN1085MU2011 | 2011-03-31 | ||
IN1085/MUM/2011 | 2011-03-31 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2012131733A2 WO2012131733A2 (en) | 2012-10-04 |
WO2012131733A3 true WO2012131733A3 (en) | 2015-06-18 |
Family
ID=46932019
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IN2012/000234 WO2012131733A2 (en) | 2011-03-31 | 2012-03-30 | Media compositions, method of initiating and enriching cultures of stem cells and/or cancer stem-like cells |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2012131733A2 (en) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2014097318A2 (en) * | 2012-12-18 | 2014-06-26 | Godavari Biorefineries Limited | Agents for eliminating tumour-initiating cells |
CN104630140A (en) * | 2013-11-06 | 2015-05-20 | 吉林济惠生物科技有限公司 | Isolation and culture method of placenta mesenchyma precursor stem cells |
JP6512662B2 (en) * | 2014-01-23 | 2019-05-15 | 日産化学株式会社 | Undifferentiated maintenance culture material |
CN103898043B (en) * | 2014-03-11 | 2016-05-11 | 山东大学附属千佛山医院 | Cell feeder layer and the application in cultivation people primary tumor cell thereof |
GB201507894D0 (en) * | 2015-05-08 | 2015-06-24 | Imagen Biotech Ltd | Personalised media |
AU2016275046B2 (en) | 2015-06-10 | 2022-07-28 | Board Of Regents, The University Of Texas System | Use of exosomes for the treatment of disease |
GB201609663D0 (en) * | 2016-06-02 | 2016-07-20 | Stemtek Therapeutics Sl | Methods for producing cancer stem cell spheroids |
US20190085284A1 (en) * | 2017-09-21 | 2019-03-21 | Codiak Biosciences, Inc. | Production of Extracellular Vesicles in Single-Cell Suspension using Chemically-Defined Cell Culture Media |
CN110878286B (en) * | 2019-12-24 | 2023-07-18 | 江苏信安佳医疗科技有限公司 | Culture medium for culturing liver cancer organoid cell balls |
CN115261325B (en) * | 2021-04-29 | 2023-12-12 | 合肥中科普瑞昇生物医药科技有限公司 | Culture medium and culture method of primary cells of oral cancer |
CN113862216B (en) * | 2021-09-17 | 2024-03-19 | 深圳市旷逸生物科技有限公司 | Purifying reagent and method for purifying cells |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040028649A1 (en) * | 2000-06-07 | 2004-02-12 | GIANNI Alessandro | Human growth hormone to stimulate mobilization of pluripotent hematopoietic stem cells |
WO2006020919A2 (en) * | 2004-08-13 | 2006-02-23 | University Of Georgia Research Foundation, Inc. | Compositions and methods for self-renewal and differentiation in human embryonic stem cells |
US20060127370A1 (en) * | 2002-10-31 | 2006-06-15 | Riken | Composition for culturing multipotent stem cells and utilization of the same |
US20100099186A1 (en) * | 2007-04-23 | 2010-04-22 | Perry John M | Methods, kits, and compositions for stem cell self-renewal |
US20100323027A1 (en) * | 2005-09-02 | 2010-12-23 | Agency For Science, Technology And Research | Mesenchymal stem cell conditioned medium |
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2012
- 2012-03-30 WO PCT/IN2012/000234 patent/WO2012131733A2/en active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040028649A1 (en) * | 2000-06-07 | 2004-02-12 | GIANNI Alessandro | Human growth hormone to stimulate mobilization of pluripotent hematopoietic stem cells |
US20060127370A1 (en) * | 2002-10-31 | 2006-06-15 | Riken | Composition for culturing multipotent stem cells and utilization of the same |
WO2006020919A2 (en) * | 2004-08-13 | 2006-02-23 | University Of Georgia Research Foundation, Inc. | Compositions and methods for self-renewal and differentiation in human embryonic stem cells |
US20100323027A1 (en) * | 2005-09-02 | 2010-12-23 | Agency For Science, Technology And Research | Mesenchymal stem cell conditioned medium |
US20100099186A1 (en) * | 2007-04-23 | 2010-04-22 | Perry John M | Methods, kits, and compositions for stem cell self-renewal |
Also Published As
Publication number | Publication date |
---|---|
WO2012131733A2 (en) | 2012-10-04 |
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