WO2012131733A3 - Media compositions for enriching cultures of stem cells - Google Patents

Media compositions for enriching cultures of stem cells Download PDF

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Publication number
WO2012131733A3
WO2012131733A3 PCT/IN2012/000234 IN2012000234W WO2012131733A3 WO 2012131733 A3 WO2012131733 A3 WO 2012131733A3 IN 2012000234 W IN2012000234 W IN 2012000234W WO 2012131733 A3 WO2012131733 A3 WO 2012131733A3
Authority
WO
WIPO (PCT)
Prior art keywords
present
culture medium
cells
stem cells
stem
Prior art date
Application number
PCT/IN2012/000234
Other languages
French (fr)
Other versions
WO2012131733A2 (en
Inventor
Martin Annette
Patil SHRUTI
Original Assignee
Godavari Biorefineries Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Godavari Biorefineries Limited filed Critical Godavari Biorefineries Limited
Publication of WO2012131733A2 publication Critical patent/WO2012131733A2/en
Publication of WO2012131733A3 publication Critical patent/WO2012131733A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Abstract

The present invention provides a culture medium for selectively enriching and maintaining stem cells or cancer stem-like cells comprising basic nutrients, gelatin, methyl cellulose and optionally growth factors or other supplementary elements in an effective amount wherein the culture medium is essentially serum free. The present invention further provides feeding medium comprising components similar to the culture medium except the feeding medium is devoid of methyl cellulose. Present invention still further provides cell cultures comprising stem cells or cancer stem-like cells and the culture medium of the present invention. Also provides are methods for selectively enriching and maintaining stem cells or cancer stem-like cells using the culture medium of the present invention and kits for the same.
PCT/IN2012/000234 2011-03-31 2012-03-30 Media compositions, method of initiating and enriching cultures of stem cells and/or cancer stem-like cells WO2012131733A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN1085MU2011 2011-03-31
IN1085/MUM/2011 2011-03-31

Publications (2)

Publication Number Publication Date
WO2012131733A2 WO2012131733A2 (en) 2012-10-04
WO2012131733A3 true WO2012131733A3 (en) 2015-06-18

Family

ID=46932019

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2012/000234 WO2012131733A2 (en) 2011-03-31 2012-03-30 Media compositions, method of initiating and enriching cultures of stem cells and/or cancer stem-like cells

Country Status (1)

Country Link
WO (1) WO2012131733A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014097318A2 (en) * 2012-12-18 2014-06-26 Godavari Biorefineries Limited Agents for eliminating tumour-initiating cells
CN104630140A (en) * 2013-11-06 2015-05-20 吉林济惠生物科技有限公司 Isolation and culture method of placenta mesenchyma precursor stem cells
JP6512662B2 (en) * 2014-01-23 2019-05-15 日産化学株式会社 Undifferentiated maintenance culture material
CN103898043B (en) * 2014-03-11 2016-05-11 山东大学附属千佛山医院 Cell feeder layer and the application in cultivation people primary tumor cell thereof
GB201507894D0 (en) * 2015-05-08 2015-06-24 Imagen Biotech Ltd Personalised media
AU2016275046B2 (en) 2015-06-10 2022-07-28 Board Of Regents, The University Of Texas System Use of exosomes for the treatment of disease
GB201609663D0 (en) * 2016-06-02 2016-07-20 Stemtek Therapeutics Sl Methods for producing cancer stem cell spheroids
US20190085284A1 (en) * 2017-09-21 2019-03-21 Codiak Biosciences, Inc. Production of Extracellular Vesicles in Single-Cell Suspension using Chemically-Defined Cell Culture Media
CN110878286B (en) * 2019-12-24 2023-07-18 江苏信安佳医疗科技有限公司 Culture medium for culturing liver cancer organoid cell balls
CN115261325B (en) * 2021-04-29 2023-12-12 合肥中科普瑞昇生物医药科技有限公司 Culture medium and culture method of primary cells of oral cancer
CN113862216B (en) * 2021-09-17 2024-03-19 深圳市旷逸生物科技有限公司 Purifying reagent and method for purifying cells

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040028649A1 (en) * 2000-06-07 2004-02-12 GIANNI Alessandro Human growth hormone to stimulate mobilization of pluripotent hematopoietic stem cells
WO2006020919A2 (en) * 2004-08-13 2006-02-23 University Of Georgia Research Foundation, Inc. Compositions and methods for self-renewal and differentiation in human embryonic stem cells
US20060127370A1 (en) * 2002-10-31 2006-06-15 Riken Composition for culturing multipotent stem cells and utilization of the same
US20100099186A1 (en) * 2007-04-23 2010-04-22 Perry John M Methods, kits, and compositions for stem cell self-renewal
US20100323027A1 (en) * 2005-09-02 2010-12-23 Agency For Science, Technology And Research Mesenchymal stem cell conditioned medium

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040028649A1 (en) * 2000-06-07 2004-02-12 GIANNI Alessandro Human growth hormone to stimulate mobilization of pluripotent hematopoietic stem cells
US20060127370A1 (en) * 2002-10-31 2006-06-15 Riken Composition for culturing multipotent stem cells and utilization of the same
WO2006020919A2 (en) * 2004-08-13 2006-02-23 University Of Georgia Research Foundation, Inc. Compositions and methods for self-renewal and differentiation in human embryonic stem cells
US20100323027A1 (en) * 2005-09-02 2010-12-23 Agency For Science, Technology And Research Mesenchymal stem cell conditioned medium
US20100099186A1 (en) * 2007-04-23 2010-04-22 Perry John M Methods, kits, and compositions for stem cell self-renewal

Also Published As

Publication number Publication date
WO2012131733A2 (en) 2012-10-04

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