WO2013067069A3 - Dual digestion method for high yield nucleic acid recovery - Google Patents
Dual digestion method for high yield nucleic acid recovery Download PDFInfo
- Publication number
- WO2013067069A3 WO2013067069A3 PCT/US2012/062884 US2012062884W WO2013067069A3 WO 2013067069 A3 WO2013067069 A3 WO 2013067069A3 US 2012062884 W US2012062884 W US 2012062884W WO 2013067069 A3 WO2013067069 A3 WO 2013067069A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- high yield
- acid recovery
- digestion method
- yield nucleic
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
Methods and kits for isolating nucleic acids from a sample are provided, comprising performing digestion of a biological sample under acidic and reduced temperature conditions, and optionally comprising performing prior digestion under alkaline conditions.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161553842P | 2011-10-31 | 2011-10-31 | |
US61/553,842 | 2011-10-31 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2013067069A2 WO2013067069A2 (en) | 2013-05-10 |
WO2013067069A3 true WO2013067069A3 (en) | 2015-06-11 |
Family
ID=48193024
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2012/062884 WO2013067069A2 (en) | 2011-10-31 | 2012-10-31 | Dual digestion method for high yield nucleic acid recovery |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2013067069A2 (en) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5386024A (en) * | 1993-02-10 | 1995-01-31 | Gen-Probe Incorporated | Method to prepare nucleic acids from a biological sample using low pH and acid protease |
US5981235A (en) * | 1996-07-29 | 1999-11-09 | Promega Corporation | Methods for isolating nucleic acids using alkaline protease |
US20040157244A1 (en) * | 2002-12-23 | 2004-08-12 | Vical Incorporated | Process for purification of plasmid DNA |
US20070160999A1 (en) * | 2004-02-04 | 2007-07-12 | Fiorella Calabrese | Method for simultaneous extraction of nucleic acids from a biological sample |
US20090182120A1 (en) * | 2005-01-21 | 2009-07-16 | Argylla Technologies, Llp | Surface mediated self-assembly of nanoparticles |
-
2012
- 2012-10-31 WO PCT/US2012/062884 patent/WO2013067069A2/en active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5386024A (en) * | 1993-02-10 | 1995-01-31 | Gen-Probe Incorporated | Method to prepare nucleic acids from a biological sample using low pH and acid protease |
US5981235A (en) * | 1996-07-29 | 1999-11-09 | Promega Corporation | Methods for isolating nucleic acids using alkaline protease |
US20040157244A1 (en) * | 2002-12-23 | 2004-08-12 | Vical Incorporated | Process for purification of plasmid DNA |
US20070160999A1 (en) * | 2004-02-04 | 2007-07-12 | Fiorella Calabrese | Method for simultaneous extraction of nucleic acids from a biological sample |
US20090182120A1 (en) * | 2005-01-21 | 2009-07-16 | Argylla Technologies, Llp | Surface mediated self-assembly of nanoparticles |
Also Published As
Publication number | Publication date |
---|---|
WO2013067069A2 (en) | 2013-05-10 |
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