WO2013188638A3 - Endoribonucleases and methods of use thereof - Google Patents

Endoribonucleases and methods of use thereof Download PDF

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Publication number
WO2013188638A3
WO2013188638A3 PCT/US2013/045602 US2013045602W WO2013188638A3 WO 2013188638 A3 WO2013188638 A3 WO 2013188638A3 US 2013045602 W US2013045602 W US 2013045602W WO 2013188638 A3 WO2013188638 A3 WO 2013188638A3
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WO
WIPO (PCT)
Prior art keywords
endoribonucleases
methods
present disclosure
nucleic acids
variant cas
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PCT/US2013/045602
Other languages
French (fr)
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WO2013188638A2 (en
Inventor
Jennifer A. Doudna
Martin Jinek
Samuel H. Sternberg
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The Regents Of The University Of California
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Publication date
Application filed by The Regents Of The University Of California filed Critical The Regents Of The University Of California
Priority to US14/403,413 priority Critical patent/US9688971B2/en
Publication of WO2013188638A2 publication Critical patent/WO2013188638A2/en
Publication of WO2013188638A3 publication Critical patent/WO2013188638A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present disclosure provides variant Cas endoribonucleases, nucleic acids encoding the variant Cas endoribonucleases, and host cells genetically modified with the nucleic acids. The variant Cas endoribonucleases find use in a variety of applications, which are also provided. The present disclosure also provides methods of detecting a specific sequence in a target polyribonucleotide; and methods of regulating production of a target RNA in a eukaryotic cell.
PCT/US2013/045602 2012-06-15 2013-06-13 Endoribonucleases and methods of use thereof WO2013188638A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/403,413 US9688971B2 (en) 2012-06-15 2013-06-13 Endoribonuclease and methods of use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261660414P 2012-06-15 2012-06-15
US61/660,414 2012-06-15

Publications (2)

Publication Number Publication Date
WO2013188638A2 WO2013188638A2 (en) 2013-12-19
WO2013188638A3 true WO2013188638A3 (en) 2014-03-20

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ID=49758893

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/045602 WO2013188638A2 (en) 2012-06-15 2013-06-13 Endoribonucleases and methods of use thereof

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US (1) US9688971B2 (en)
WO (1) WO2013188638A2 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11970711B2 (en) 2021-09-21 2024-04-30 The Regents Of The University Of California Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription

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US20150152398A1 (en) 2015-06-04
US9688971B2 (en) 2017-06-27
WO2013188638A2 (en) 2013-12-19

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