WO2014035547A3 - Methods of isolating nucleic acids under reduced degradation condition - Google Patents

Methods of isolating nucleic acids under reduced degradation condition Download PDF

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Publication number
WO2014035547A3
WO2014035547A3 PCT/US2013/048853 US2013048853W WO2014035547A3 WO 2014035547 A3 WO2014035547 A3 WO 2014035547A3 US 2013048853 W US2013048853 W US 2013048853W WO 2014035547 A3 WO2014035547 A3 WO 2014035547A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
methods
under reduced
isolating nucleic
acids under
Prior art date
Application number
PCT/US2013/048853
Other languages
French (fr)
Other versions
WO2014035547A2 (en
Inventor
John Richard Nelson
Li Zhu
Erin Jean FINEHOUT
Xiaohui Chen
Kashan Ali Shaikh
Christopher Michael PULEO
Patrick McCoy SPOONER
Original Assignee
General Electric Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/599,124 external-priority patent/US20140039177A1/en
Application filed by General Electric Company filed Critical General Electric Company
Priority to GB1503908.4A priority Critical patent/GB2519910B/en
Publication of WO2014035547A2 publication Critical patent/WO2014035547A2/en
Publication of WO2014035547A3 publication Critical patent/WO2014035547A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Abstract

A method of isolating nucleic acids from a biological material, comprises applying the biological material on a substrate comprising one or more cell lysis reagents impregnated therein; applying a fluid to the biological material applied on the substrate; extracting the nucleic acids from the biological material applied on the substrate; and collecting the extracted nucleic acids in a substantially intact form, wherein the collected nucleic acid has a molecular weight greater than or equal to 20 kb.
PCT/US2013/048853 2012-08-30 2013-07-01 Methods of isolating nucleic acids under reduced degradation condition WO2014035547A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB1503908.4A GB2519910B (en) 2012-08-30 2013-07-01 Methods of isolating nucleic acids under reduced degradation condition

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/599,124 2012-08-30
US13/599,124 US20140039177A1 (en) 2012-07-31 2012-08-30 Methods of isolating nucleic acids under reduced degradation condition

Publications (2)

Publication Number Publication Date
WO2014035547A2 WO2014035547A2 (en) 2014-03-06
WO2014035547A3 true WO2014035547A3 (en) 2015-07-23

Family

ID=50184574

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/048853 WO2014035547A2 (en) 2012-08-30 2013-07-01 Methods of isolating nucleic acids under reduced degradation condition

Country Status (2)

Country Link
GB (2) GB2552110B (en)
WO (1) WO2014035547A2 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999022868A1 (en) * 1997-10-31 1999-05-14 Bbi Bioseq, Inc. Pressure-enhanced extraction and purification
US6555945B1 (en) * 1999-02-25 2003-04-29 Alliedsignal Inc. Actuators using double-layer charging of high surface area materials
WO2006132666A1 (en) * 2005-06-06 2006-12-14 Decision Biomarkers, Inc. Assays based on liquid flow over arrays
WO2007011919A2 (en) * 2005-07-15 2007-01-25 Microlin, Llc Dual membrane electro-osmotic fluid delivery device
US20110097215A1 (en) * 2009-10-23 2011-04-28 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Flexible Solid-State Pump Constructed of Surface-Modified Glass Fiber Filters and Metal Mesh Electrodes
WO2012082849A1 (en) * 2010-12-17 2012-06-21 General Electric Company Method for electroeluting genetic material from dried samples

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6572830B1 (en) * 1998-10-09 2003-06-03 Motorola, Inc. Integrated multilayered microfludic devices and methods for making the same
WO2004005553A1 (en) * 2002-07-10 2004-01-15 Massachusetts Institute Of Technology Apparatus and method for isolating a nucleic acid from a sample

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999022868A1 (en) * 1997-10-31 1999-05-14 Bbi Bioseq, Inc. Pressure-enhanced extraction and purification
US6555945B1 (en) * 1999-02-25 2003-04-29 Alliedsignal Inc. Actuators using double-layer charging of high surface area materials
WO2006132666A1 (en) * 2005-06-06 2006-12-14 Decision Biomarkers, Inc. Assays based on liquid flow over arrays
WO2007011919A2 (en) * 2005-07-15 2007-01-25 Microlin, Llc Dual membrane electro-osmotic fluid delivery device
US20110097215A1 (en) * 2009-10-23 2011-04-28 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Flexible Solid-State Pump Constructed of Surface-Modified Glass Fiber Filters and Metal Mesh Electrodes
WO2012082849A1 (en) * 2010-12-17 2012-06-21 General Electric Company Method for electroeluting genetic material from dried samples

Also Published As

Publication number Publication date
GB2519910A (en) 2015-05-06
GB201503908D0 (en) 2015-04-22
GB2552110B (en) 2018-05-23
WO2014035547A2 (en) 2014-03-06
GB2552110A (en) 2018-01-10
GB2519910B (en) 2018-05-09
GB201714375D0 (en) 2017-10-25

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