WO2015072718A1

WO2015072718A1 - Multi-well cuvette provided with integrated reaction and detection means

Info

Publication number: WO2015072718A1
Application number: PCT/KR2014/010784
Authority: WO
Inventors: 김병철; 문봉석; 이원희; 전찬영
Original assignee: 바디텍메드 주식회사
Priority date: 2013-11-12
Filing date: 2014-11-11
Publication date: 2015-05-21

Abstract

The present application discloses a multi-well cuvette for integrating the reaction between a sample and a reagent with the detection of a specific analyte included therein, and the multi-well cuvette comprises: a reaction chamber part having a sample and a reagent placed therein; and a detection part for detecting the reaction between the sample and the reagent, wherein the reaction chamber part comprises: an extraction member standby chamber in which a member for dividing or distributing the sample is on standby; a sample filling chamber; and a reagent filling chamber which is filled with the reagent, and in which the reaction with the sample is carried out, wherein the detection part comprises: a chromatography analysis means for a reactive product between the sample and the reagent; and a detection chamber for accommodating the same.

Description

Multiwell cuvettes with integrated reaction and detection means

The present invention relates to multiwell cuvettes used for the detection and analysis of certain components in a sample, such as a biological sample.

With the development of medicine and various related technologies, the testing of substances such as blood cells, nucleic acids, proteins and antigens contained in certain biological samples such as blood and the like has been made. After taking the sample as described above, by analyzing and observing the change that occurs after reacting the sample with a predetermined reagent, the presence or absence of various substances contained in the sample, the ratio, and the amount can be examined, accordingly Information on the presence or absence of a disease, the condition of the disease, and the like can be obtained.

In the inspection of such a sample, the sample and the reagents used for the inspection of the sample are not influenced by external factors, and using the correct amount each time is very important for obtaining accurate and reproducible results. In the course of inspection, the samples and reagents may be exposed to the outside, so it is necessary to effectively prevent exposure of such samples and reagents and to use the correct amount to ensure the accuracy of the inspection.

In addition, in the case of the conventional test, the detection means for detecting the reaction of the reagent and the sample for each test item and the reaction result thereafter are performed in separate apparatuses, thereby causing frequent misuse such as incorporation between reagents and use of the wrong amount. There was a problem.

Therefore, after the reaction between the reagent and the sample, the test for the detection of the reaction result is carried out in a single apparatus to reduce the test cost, the accuracy of the test as well as the steps included in the test and the cost to be added need.

[Preceding technical literature]

[Patent Documents]

(Patent Document 1) Republic of Korea Patent Application 10-2007-7031051

SUMMARY OF THE INVENTION The present invention has been made to solve the above-described problems, and it is an object of the present invention to provide a test apparatus capable of performing reaction and detection of a sample and a reagent in one apparatus.

In order to achieve the above object, the present application provides a multi-well cuvette having an integrated reaction and detection means, the multi-well cuvette according to the present invention, in the multi-well cuvette to examine the reaction between the sample and the reagent, A reaction chamber portion in which a sample and a reagent are placed; And a detector configured to detect a reaction between the sample and the reagent, wherein the reaction chamber includes: a sampling member waiting chamber in which a member for dispensing or dispensing a sample stands by; One or more sample filling chambers; And one or more reagent filling chambers in which a reagent is filled and a reaction with a sample is performed, wherein the detection unit includes a chromatographic analysis means for a reaction product between the sample and the reagent and a detection chamber accommodating the same. .

In one embodiment according to the present invention, the multiwell cuvette includes the collecting member atmospheric chamber, the reagent filling chamber and the detection chamber included therein in order to have at least one row.

In one embodiment according to the present invention, the chromatographic analysis means includes, but is not limited to, lateral flow analysis means, the lateral flow analysis means is a lateral flow analysis cartridge, the cartridge is a base member; And a cover member engaged with the base member, wherein the base member includes a strip receiving portion for receiving a strip used for the lateral flow analysis and a reaction result well formed at a position extending from one end of the strip receiving portion The cover member may include a measuring window formed in a portion corresponding to the strip receiving portion and an inlet formed in a portion corresponding to the reaction result well when the cover member is filled with the base member, or the base member or the cover member, or The base member and the cover member include a capillary structure for delivering the reaction product to the strip, wherein the capillary structure formed on the cover member is formed on a lower surface adjacent to the inlet of the cover member, The formed capillary structure is formed in the sample well. It will, may further comprise a strip that is accommodated in the strip accommodation portion for analysis.

In one embodiment according to the present application, the multiwell cuvette of the present application further comprises a separation unit located at the bottom of the reaction product well of the means for lateral flow analysis. The separating part is used to separate impurities contained in the reaction product, for example, including a magnet part. The magnet part is composed of a magnet holder and a space in which the magnet is inserted, and is used to remove impurities contained in the sample to be analyzed.

In one embodiment according to the present application, the collecting member atmospheric chamber and the reagent filling chamber included in the multi-well cuvette of the present application includes an open portion formed at an upper portion thereof so that inspection equipment having a predetermined tip may be introduced.

In one embodiment according to the present application, two or more sample filling chambers and / or reagent filling chambers of the multiwellcuvet herein may be formed, and when two or more are filled, each chamber contains the same or different samples and reagents. can do.

In one embodiment according to the present application the multi-well cuvette of the present application may further comprise a first cover covering the top surface of the reagent filling chamber. In one embodiment, the first cover has a thin film structure such that inspection equipment having a predetermined tip penetrates through at least a portion thereof.

Furthermore, in one embodiment according to the present application, the multi-well cuvette of the present invention may include a second cover that covers all or part of the upper surface of the multi-cube, and in one embodiment, the cover is used with the cover mounted.

Meltiwell cuvettes according to the present disclosure further comprise a barcode in any position of the second cover in one embodiment.

When performing the detection and analysis of the specific components contained in the sample using the multi-well cuvette according to the present invention, the inspection can be made with only a quick and simple operation as well as accuracy. That is, a sample filled in the sample filling chamber is collected by using a collecting member in the collecting member chamber by using a predetermined test equipment, and then reacted with the reagent filled in the reagent filling chamber. Then, specific components included in the reaction product are quantitatively and / or qualitatively analyzed by chromatographic methods.

All of these inspections are carried out on one multiwell cuvette including the configuration formed integrally and sequentially arranged in one direction, and when used with the inspection equipment, the inspection is carried out by displacing the cuvette on a straight line. Can be done.

In addition, as the collecting member atmospheric chamber, the reagent filling chamber, and the detection chamber are sequentially disposed, the sampling, the mixing of the reagent and the sample, the reaction between the reagent and the sample, and the detection of the reaction result may be sequentially performed. In the process, the positional change of the inspection equipment may be simply made in one direction.

Therefore, when performing the inspection using the multi-well cuvette according to the present invention, the structure of the inspection equipment is simple and the inspection through this can also be made easily and quickly.

In addition, the multiwell cuvette according to the present invention has a structure in which the collecting member atmospheric chamber, the reagent filling chamber, and the detection chamber are integrally formed and arranged in one direction so that the structure of the manufacturing apparatus used for manufacturing is simple. It can be simplified and the manufacturing cost can be reduced.

In addition, when the test is performed using the multi-well cuvette according to the present invention, the reagent chamber and the detection chamber are integrated, and in the case of the conventional chromatography test, the reagent and the detection cartridge are separately supplied for each test item. This overcomes the disadvantages of frequent misuse, such as mixing between reagents or using the wrong amount.

1 is a perspective view schematically showing a multiwell cuvette according to an embodiment of the present invention.

2 is an exploded perspective view schematically showing a multiwell cuvette according to an embodiment of the present invention.

3 is a diagram illustrating a process of performing a test using a multiwell cuvette according to an embodiment of the present invention.

Figure 4 is an exploded perspective view schematically showing a cartridge for lateral flow analysis included in the multi-well cuvette according to an embodiment of the present invention.

5A is a perspective view schematically illustrating a multiwell cuvette including a second cover according to an embodiment of the present invention.

FIG. 5B is a perspective view schematically illustrating a multiwell cuvette with the second cover of FIG. 5A partially removed.

6 is a cross-sectional view taken along line AA ′ of FIG. 5A.

Hereinafter, exemplary embodiments of the present invention will be described with reference to the accompanying drawings. This example is illustrative and does not limit the invention in any way.

The spatially relative terms "bottom", "back", "top", "top", etc., as shown in the figures, facilitate the correlation of one element or component with another element or components. Can be used to describe. Spatially relative terms are to be understood as terms that include different directions of the device in use or operation in addition to the directions shown in the figures. For example, when flipping the member shown in the figure, the member described as "below" or "below" of the other member may be placed "above" of the other member. Thus, the exemplary term "below" can encompass both an orientation of above and below. The member can also be oriented in other directions, so that spatially relative terms can be interpreted according to the orientation. For example, "horizontal direction" may also be interpreted as "up and down direction", but is not limited thereto.

In the drawings, the thickness or size of each member is exaggerated, omitted, or schematically illustrated for convenience and clarity of description. In addition, the size and area of each component does not necessarily reflect the actual size or area.

In addition, the angle and direction mentioned in the process of demonstrating the structure of this invention in the Example are based on what was described in drawing. In the description of the structure constituting the present invention in the specification, if the reference point and the positional relationship with respect to the angle is not clearly mentioned, reference is made to related drawings.

1 is a perspective view of an integrated multiwell cuvette 1 according to one embodiment of the invention, and FIG. 2 is an exploded perspective view of an integrated multiwell cuvette 1 of the present invention.

Cuvettes according to the invention are used for the detection and / or analysis of analytes contained in a sample. As used herein, the term “sample” refers to an analyte or composition that includes an analyte, and a sample that may be used in the present invention is a liquid phase or liquid like material. In one embodiment according to the present application, the sample is a biological sample, and may be a body-derived body component such as whole blood, plasma, serum, urine, saliva, manure and cell extracts.

As used herein, the term “analyte” is a compound of interest in a sample, also referred to as a target, that includes proteins and nucleic acids. Proteins herein include polypeptides and peptides. The term “nucleic acid” herein also includes chemical synthetic DNA and RNA, including genome DNA, cDNA and oligonucleotides. In addition, the nucleic acid includes single- or double-stranded, genome DNA, cDNA and oligonucleotides and RNA can be prepared or synthesized according to methods known in the art.

In one embodiment according to the present invention, a protein in a sample, for example, high sensitivity C-reactive protein (hsCRP), MicroCRP, HbA1c (glycosylated hemoglobin), microalbumin, PSA (prostate specific antigen), AFP (Alpha-fetoprotein), cTnI (Cardiac) Used to detect Troponin I).

Reagents herein are materials suitable for the detection and analysis of the analytes described above, depending on the specific analyte, for example, the reagents may be any antibody or the like that reacts with various substances in the body component, eg, antigens, and the like. It is not limited to this.

Multi-well cuvette 1 according to the present invention, the reaction chamber unit 100 for receiving a sample and a reagent; And a detection unit 200 for detecting a reaction between the sample and the reagent, wherein the chamber unit 100 includes: a collecting member waiting chamber 110 in which a member for dispensing or dispensing the sample stands by; A sample filling chamber 120 and a reagent filling chamber, and a reagent filling chamber 130 in which a reaction between the sample and the reagent is performed, wherein the detection unit is chromatographic analysis of the reaction product between the sample and the reagent. Means for detection and detection chamber 210 containing the same.

The reaction chamber part 100 included in the multiwell cuvette 1 according to the present invention is configured to have a plurality of chambers for accommodating various samples used for the test and predetermined reagents reacting with the samples. Accordingly, the chamber part 100 may have a structure having a barrier for preventing unnecessary mixing between reagents and samples by separating the plurality of wells and the wells. That is, the reaction chamber part 100 may have a predetermined multi-well structure. On the other hand, the chamber formed in the reaction chamber unit 100 may be a plurality, the number is not limited.

The reaction chamber unit 100 according to the present invention includes a collecting member atmospheric chamber 110 for dispensing or dispensing a sample, a sample filling chamber 120 for receiving a sample, and a reagent filling chamber 130 for filling a reagent. Can be configured. As described above, the sampling member atmospheric chamber 110 and the sample filling chamber 120 and the reagent filling chamber 130 in which the sample is filled are configured in a well structure separated by a predetermined barrier, respectively, so that the collecting member atmospheric chamber is formed. 110, the reagent filling chamber 120 and the reagent filling chamber 130 may have a structure that prevents unnecessary mixing of the reagent and the sample. The reagent filling chamber 130 may include a reagent suitable for detection and / or analysis thereof according to the type of analyte, and when a sample is introduced from the sample filling chamber 120, a reaction between the sample and the reagent introduced in the reagent filling chamber is performed. This is done. In addition, the reagent filling chamber 130 may be formed in plural to be filled with the same or different types of samples and reagents, respectively, but in the case of a plurality of reagent filling chambers, not all of the reagent filling chambers should include reagents, only a part of them. Of course, it can also be used. One embodiment according to the present invention includes, but is not limited to, the first reagent filling chamber 130 and the second reagent filling chamber 132.

The detection unit 200 according to the present invention includes a detection chamber 210 including chromatographic analysis means suitable for chromatographic analysis of the reaction product between the sample and the reagent performed in the reaction chamber unit 100. Accordingly, the detection unit 200 may include a means suitable for chromatographic analysis of reactants, for example, lateral flow analysis. Preferably, the detection chamber 210 may comprise a cartridge, for example for a lateral flow assay.

Lateral flow analysis is a method of quantitatively or qualitatively examining a target analyte, eg, a particular nucleic acid or protein, contained in a sample, e.g., an oligonucleotide or a specific antibody and / or antigen that hybridizes to a nucleic acid of a constant sequence. A chromatographic method comprising a nitrocellulose membrane (development medium) called strip 20 bound to a specific position is used to transfer the analyte in the reaction product to identify the sample in the sample through sequence specific hybridization or antigen antibody reactions. It is a method for detecting a nucleic acid or protein. For example, reference may be made to those disclosed in Korean Unexamined Patent Publication Nos. 2003-0065341, 2011-0007699, 2011-0127386, and Registration No. 1149357.

Thus, referring to FIG. 2, in one embodiment according to the present disclosure, the detection chamber 210 comprises a cartridge suitable for lateral flow analysis, the cartridge comprising: a strip receiving portion for receiving a strip comprising a development medium; And a base member formed on one end side of the strip receiving portion in a position extending from the strip receiving portion, and formed with a well for receiving a reaction product of the reaction chamber portion, and a cover member engaged with the base member. For example, reference may be made to those disclosed in Korean Unexamined Patent Publication Nos. 2003-0065341, 2011-0007699, 2011-0127386, and Registration No. 1149357.

For example, the detection chamber according to the present application is a chromatographic analysis means of the reaction result, and includes a cartridge for lateral flow analysis as disclosed in, for example, Korean Patent Publication No. 2011-0046833 or FIG. 4.

Referring to FIG. 4, the cartridge 220 is installed in the detection chamber 210 and includes a cover member 30 and a base member 10. The reaction product is introduced into the well 12 through the inlet 32, and the triangular

capillary structures

35 and 37 and the base member are formed on the lower surface of the cover member to transfer the reaction product from the well to the strip 20. The corresponding structure is formed. Specifically, the lateral flow analysis cartridge included in the cuvette of the present application includes a base member 10; And a cover member 30 engaged with the base member, the base member having a strip receiving portion 18 for receiving a strip used for the lateral flow analysis, and a position extending from one end of the strip receiving portion. And a sample well 12 formed in the cover member, wherein the cover member includes a measurement window 34 and a sample well for detecting a reaction result formed at a portion corresponding to the strip accommodating portion when the base member is filled with the base member. A sample inlet 32 formed in a corresponding portion may be included, and if necessary, an air window 38 may be further included. The base member or the cover member, or the base member and the cover member includes

capillary structures

35 and 37, wherein the capillary structure formed on the cover member is formed on a lower surface adjacent to the sample inlet of the cover member, The capillary structure formed on the base member is formed in the sample well.

The sample inlet 32 is formed to be perpendicular to the well containing the reaction product. Covering the cover member 30 on the base member is interlocked with each other at its interface via a fastening means including a locking jaw and an uneven protrusion 16, thereby substantially waterproof and aerosol proof. ) It becomes a seal. The strip receptacle also includes a guide for receiving a plurality of strips to prevent the mounted strip from shaking and to position the strip in a fixed position.

The reaction product introduced into the inlet 32 is moved to the strip 20 through various methods. For example, the lateral flow cartridge may have a predetermined microchannel structure formed between the well formed at a position perpendicular to the inlet and the strip receiving portion 18, and through the capillary phenomenon, the reaction product of the well is transferred to the strip. And lateral flow is disclosed, but not limited thereto. In another example, one end of the strip is in direct contact with the well to absorb a liquid sample into the strip to initiate lateral flow, but is not limited thereto.

The multi-well cuvette according to the present invention may further include a magnet part 138 located at the bottom of the well containing the reaction product constituting the lateral flow cartridge. The magnet part is composed of a magnet 140 and a well 142 for accommodating the magnet 140. The magnet is for removing impurities contained in the reaction product to be analyzed. To this end, the reagent filling chamber 130 includes a magnetic bead, and an antibody capable of specifically binding to impurities to be removed is bound to the magnetic bead. For example, impurities may include red blood cells contained in whole blood, and after reaction with samples and reagents, reaction products may include magnetic beads capable of reacting with a magnet and antibodies specific to impurities bound to the magnetic beads, such as red blood cells. For erythrocyte antibodies are bound. Thereafter, the red blood cells in the reaction product are bound to the magnetic beads through the antibody, and the magnetic beads located in the lower part of the well containing the reaction product settle to the bottom when contacted with the magnet 140 and do not move to the strip, resulting in analysis. It doesn't work.

5A and 5B, the cuvette 1 according to the present disclosure may further include a second cover 136. The second cover 136 covers the upper end of the cuvette and covers all or part of the reaction chamber 100 and the detection unit 200 to identify and protect the surface of the product. In this case, as shown in FIG. 5B, a first cover 134 covering the first reagent filling chamber and the second reagent filling chamber is located below the second cover. As described below, the second cover is provided for dispensing or collecting the liquid sample provided in the equipment used with the cuvette according to the present application, for example, for entering or exiting the tip T or reading the detection result (in the case of a measuring window). Corresponding to the sample inlet 32 and the measurement window 34 formed in the collecting member atmospheric chamber 110, the sample filling chamber 120, the first reagent filling chamber 132, the second reagent filling chamber 132, and the cartridge. In order to expose the parts, a hole of appropriate shape is formed in each of the corresponding parts. In addition, the second cover can be peeled off if necessary as shown in FIG. 5B, but is normally used in a state in which the second cover is present.

In one embodiment according to the present application, the second cover may further include a barcode 400 at any position thereof. Meltiwell cuvettes according to the present application are used in conjunction with certain inspection equipment as described below, such as automated equipment in which the dispensing of a sample, the reaction between a sample and a reagent, and the detection of a reaction result are performed automatically in one equipment. In this case, the material or sample to be measured or analyzed by using a multi-well cuvette is made to match the ID chip inserted into the predetermined inspection equipment, and the ID chip contains information of the material to be measured and quantification thereof accordingly. Standard curve information, and information on error processing when the range is out of range are stored.

Cartridges mounted and used in the cuvette according to the present application may be manufactured in various sizes and shapes suitable for mounting and use in the detection chamber 210, may be manufactured in a rectangular shape and such a rectangle of the cuvette according to the present application It can be used in a variety of measuring equipment (readers) that can accommodate cartridges in the form, and can be made from chemically stable synthetic resins and combinations thereof.

As shown in FIG. 1, the multiwell cuvette 1 according to the present invention includes a collecting member atmospheric chamber 110, a sample filling chamber 120, a

reagent filling chamber

130, 132, and a detection chamber 210. It is formed integrally on the multiwell cuvette 1, and are sequentially arranged in one direction to form heat. In this case, although not shown in FIG. 1, a plurality of rows formed by the collecting member atmospheric chamber 110, the reagent filling chamber 130, and the detection chamber 210 may be plural, but is not limited thereto.

The multiwell cuvette 1 according to the invention having the structure as described above can be used with certain inspection equipment. For example, the test equipment may be an automated equipment in which a dispensing of a sample, a reaction between a sample and a reagent, and detection of a reaction result are automatically performed in one equipment.

With the arrangement arrangement as described above, when the inspection is performed using the multi-well cuvette 1 according to the present invention, the inspection can be made faster and simpler operation with only a linear displacement. In addition, since the reaction filling chamber including the reaction reagent is integrally structured in the detecting means, a separate reagent container is not required, thereby reducing the probability of erroneous inspection due to confusion of reagents during a plurality of different tests. In addition, when multiple reagents, which are the same or different, are included in the reaction filling chamber, this feature allows the detection of various analytes without separate reagent replacement in one instrument.

In addition, in the multi-well cuvette 1 according to the present invention, the collecting member atmospheric chamber 110, the sample filling chamber 120, the reagent filling chamber 130, and the detection chamber 210 are integrally formed in one direction. Since the structure of the arrangement is simple, the structure of the manufacturing apparatus used for manufacturing is simplified and the manufacturing cost can be reduced.

The cuvettes according to the present application are used for single use and include the following disposable microtips (e.g. 2-1000μl micropipette tips) used in practice and with means for dispensing reagents and dispensing, thus providing a separate reagent supply device and contamination. Can be used with equipment that does not have a means to clean the equipment, simplifying the operation of the equipment.

For example, the predetermined test equipment is an automated equipment in which the distribution of a sample and a reaction result and the detection of an analyte in the reaction result may be automated. The automated equipment may include a means for distributing or dispensing a sample and a reaction result. . Dispensing or dispensing means included in the inspection equipment is the collecting member atmospheric chamber 110, the sample filling chamber 120, the reagent filling chamber 130 and the cartridge 230 of the detection unit 200 of the cuvette (1) according to the present application It is movable on the X axis along the inlet 32 formed in the inlet, and includes an arm equipped with a pump for suction and discharge of the liquid material for dispensing or dispensing a sample, a reagent, or a reaction product. The end of the tip is formed. The tip is inserted into a harvesting member, eg, 10 µl, 100 µl, 200 µl, or 500 µl volume microtip, located in the harvesting member atmospheric chamber 110, for use in dispensing and / or dispensing samples, reagents, and / or reaction products. . The collecting member standby chamber 110 is formed so that the upper portion is opened so that the inspection equipment having a predetermined tip is input as described above to collect a sample. That is,

open portions

112 and 114 may be formed on the upper surfaces of the collecting member atmospheric chamber 110 and the sample filling chamber 120 to allow the tip to be introduced. Of course, the microtips may be mounted through the

openings

112 and 114 and a predetermined sample may be dispensed.

In one embodiment according to the present invention, the multiwell cuvette 1 according to the present invention may include a cover covering the reagent filling chamber 130 and the upper surface of the detection chamber 210.

The cover covers at least a portion of the reagent filling chamber 130 and the detection chamber 210 and at least partially isolates the inner space covered by the cover to the outside. Accordingly, the reagent filled in the reagent filling chamber 130 and the mixture of the reagent and the sample placed in the detection chamber 210 may be prevented from being altered or inadvertently reacted by an external environment, thereby improving test reliability. Can be.

In this case, preferably, a first cover 134 is disposed on the reagent filling chamber 130, and the first cover 134 has a thin film structure so that inspection equipment having a predetermined tip penetrates at least a portion thereof. It can have That is, a first cover 134 having a thin thin film structure is disposed on the reagent filling chamber 130, and the first cover 134 is drilled by a tip equipped with a sampling member provided in the inspection equipment, and the collecting member is disposed. It may have a configuration that can be penetrated with a tip. For example, the first cover 134 may be a thin film made of a metal such as aluminum, or a thin film made of a synthetic resin such as plastic, but is not limited thereto.

Preferably, a predetermined connection jig 300 may be provided at one end. The connecting jig 300 is disposed at one end of the multi-well cuvette 1 according to the present invention, and may be provided at one end in the longitudinal direction as shown in FIG. 1, but is not limited thereto. Can be placed in.

As the connecting jig 300 is provided, replacement or disposal may be easily performed after the inspection is performed using the multi-well cuvette 1 according to the present invention. That is, an easy inspection can be performed by detachably connecting the multiwell cuvette 1 according to the present invention to the predetermined inspection apparatus using the connection jig 300.

3 is a view showing performing a predetermined test using the multi-well cuvette 1 according to the present invention.

First, the tip T provided in the predetermined inspection equipment S mounts the micro tip of the collecting member atmospheric chamber 110 through the open part 112, and then moves to the X axis, so that the sample as shown in (A). Take a sample filled in the filling chamber.

Next, the inspection equipment (S) is moved as shown in (B) to dispense a sample into the reagent filling chamber 130 to react the reagent with the sample. In this case, the collected sample is administered in the reagent filling chamber 130 to react.

Subsequently, as in (C), the reaction product of the reactant and the sample is administered to the detection chamber 210 through an inlet. At this time, the detection chamber 210 may be provided with a cartridge having a strip 20 for lateral flow as described above, the analysis result in the chromatography is detected through the reader of the equipment (S) through the measurement window .

Although the preferred embodiments have been illustrated and described above, the invention is not limited to the specific embodiments described above, and does not depart from the gist of the invention as claimed in the claims. Various modifications may be made by the person having the above, and these modifications should not be individually understood from the technical spirit or the prospect of the present invention.

Although the exemplary embodiments of the present application have been described in detail above, the scope of the present application is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concepts of the present invention defined in the following claims are also provided. It belongs to.

All technical terms used in the present invention, unless defined otherwise, are used in the meaning as commonly understood by those skilled in the art in the related field of the present invention. The contents of all publications described herein by reference are incorporated into the present invention.

[Description of the code]

1: cuvette

100: reaction chamber portion

110: collecting member atmospheric chamber

112, 114: open section

120: sample filling chamber

130: first reagent filling chamber

132: second reagent filling chamber

134: first cover

136: second cover

138: separator or magnet

140: magnet

142: magnet holder

200: detector

210: detection chamber

220: cartridge

300: connecting jig

400: barcode

Claims

A multiwell cuvette having integrated reaction and detection means,

A reaction chamber portion in which a sample and a reagent are placed; And

Including; detection unit for detecting a reaction between the sample and the reagent,

The reaction chamber portion,

A collecting member waiting chamber in which a member for dispensing or dispensing a sample waits; A sample filling chamber; And at least one reagent filling chamber in which a reagent is filled and a reaction with the sample is performed.

The detection unit,

And means for chromatographic analysis of the reaction product between the sample and the reagent and a detection chamber therein.
The method of claim 1,

The means for chromatographic analysis is a cartridge for lateral flow analysis, multi-well cuvette.
3. The lateral flow analysis cartridge according to claim 2, further comprising: a base member; And a cover member engaged with the base member,

The base member includes a strip receiving portion containing a strip used for the lateral flow analysis and a reaction result well formed at a position extending from one end of the strip receiving portion,

The cover member includes a measuring window formed at a portion corresponding to the strip receiving portion and an opening formed at a portion corresponding to the reaction result well when the base member is filled with the base member.

The base member or the cover member, or the base member and the cover member includes a capillary structure for delivering the reaction product to the strip, wherein the capillary structure formed on the cover member is adjacent to the inlet of the cover member. Is formed on the lower surface, the capillary structure formed in the base member is formed in the sample well, multi-well cuvette.
4. The multi-well cuvette of claim 3, wherein the multiwell cuvette further comprises a magnet located under the reaction well.
The method of claim 1,

The collection member atmospheric chamber, the reagent filling chamber and the detection chamber are arranged in sequence in one direction to have at least one row, multi-well cuvette.
The method of claim 1,

And the collecting member atmospheric chamber and the reagent filling chamber have an open portion formed at an upper portion thereof so that inspection equipment having a predetermined tip can be inserted therein.
The method of claim 1,

Wherein at least two reagent filling chambers are formed.
The method of claim 1,

And a first cover covering a top surface of said reagent filling chamber.
The method of claim 1,

And the first cover has a thin film structure such that inspection equipment having a predetermined tip penetrates through at least a portion thereof.
The method of claim 1,

And the cuvette comprises a second cover covering all or part of its top surface.
The multiwell cuvette of claim 10, wherein the second cover further comprises a barcode that identifies the type of sample to be analyzed using the Meltiwell cuvette.