WO2015092719A1 - Aqueous compositions of copper complexes, polymeric copper complexes and aloe vera - Google Patents

Abstract

The invention relates to an aqueous composition based on a polymeric copper complex and aloe vera, comprising a bactericidal and fungicidal copper complex. The invention also relates to the use of a polymeric copper complex and aloe vera in order to produce a composition that can be used in the treatment of wounds, in the prevention of mastitis in cows, such as in post-milking dipping, and as a wound cicatrizant, in the treatment of fungi on animals.

Description

 DESCRIPTIVE MEMORY

 Aqueous compositions of copper complexes, polymer copper complexes and aloe vera

 FIELD OF THE INVENTION

 The present invention describes aqueous compositions based on copper and Aloe Vera complexes. These complexes have a marked bactericidal and fungicidal power. When mixing these copper complexes with aloe vera gel, we observe that they maintain their bactericidal and fungicidal power and additionally showed a remarkable healing effect when applied to wounds in animals and humans. The use of the compositions for animal use is also described, in particular as pre-dipping and dipping for cow udders.

 BACKGROUND OF THE INVENTION

 Since ancient times, the fungicidal and bactericidal capacity of copper and its salts was known empirically, as well as the healing capacity of aloe vera. The fundamental concept of this composition is the synergy observed when the two products are used together.

 The fungicidal and bactericidal capacities of cupric salts are known, however it is also known that soluble copper salts such as copper sulfate are phytotoxic even if they are still used at low doses such as 100 ppm. expressed as metallic copper.

The original purpose of this development was to formulate a dipping for the tits of the cows based on copper. In the art of dippings formulation, most of the formulations contain the bactericidal element in a typical concentration between 1500 and 10,000 ppm, as is the case for example of iodinated dippings. This type of product is characterized because virtually all of the available iodine is complexed with organic molecules such as with 12M ethoxylated nonylphenol.

 In equilibrium a small amount of the available iodine is in the state of free or active iodine (2-20ppm). This small amount of free iodine is responsible for the bactericidal properties of these products. To the extent that free iodine acts as an oxidant on bacteria, free iodine is consumed, the ethoxylated mud-Nonylphenol complex spontaneously releases a small amount of iodine to re-establish equilibrium. In this way, it is possible to maintain a residual of free or active iodine for a longer time since there is reserve iodine as a complex that will provide free iodine as it is consumed. If all the iodine available in the dipping was as free iodine, the product would be very irritating and would not be usable as dipping.

 This same concept was used in the development of a dipping based on copper salts. We look in the literature for copper complexes suitable for this development.

 Spanish patent ES 2 125 947 T3 published on October 13, 1993, refers to a bactericidal and fungicidal composition for the treatment of fungal and bacterial diseases based on a copper complex with semi-neutralized polycarboxylic acids, which has the particularity of not be phytotoxic, being able to apply on the foliage of plants and trees even in high concentrations without damaging the leaves.

This quality is precisely what we are looking for, that is, forming a copper complex that has a small amount of free Cu ++ in equilibrium, responsible for germicidal capacity. The reasoning was that if these complexes were not phytotoxic, it is because most of the copper is complexed Therefore, they would not be toxic or irritating to animal tissues.

 In a first stage the product was developed oriented to its use as dipping post milking to prevent mastitis. Aloe vera was used as a healing aid, since we have observed that always within the dairy herds there are cows with nipples with some degree of wounds, be they due to trauma, irritations, low temperatures etc. Nipple wounds are a source of colonization of bacteria, whether they are contagious or environmental.

 Mastitis is the most common and most expensive disease that affects milking herds.

 It has been estimated that at least 50% of the population of animals in milking has some degree or form of mastitis. This condition produces a decrease in production yields and also reduces milk quality.

 Mastitis is an inflammation of the mammary gland. Inflammation is a response of tissues or organs that have been damaged. Tissue damage can be caused by an aggression, which can be physical, chemical, thermal, causing trauma to the tissues and will produce an inflammatory response.

 In milking cows, mastitis is caused by microorganisms, usually bacteria that invade the udder, multiply in milk-producing tissues. These bacteria produce toxins due to their metabolism. Typical characteristics of inflammation include swelling, heat, redness, pain and decreased tissue function (less milk production).

While the animals' immune system can act on intramammary infections, many chronic infections remain sub-clinical (asymptomatic) and are not detected unless a diagnosis is made in a clinical laboratory. Subclinical mastitis can be a reservoir of microorganisms that leads to infection of other animals within the herd due to contamination during milking.

 Approximately 95% of mastitis is caused by 4 pathogens: staphylococcus aureus, streptpcoccus agalactiae, streptococcus dysagalacteae and strptococcus uberis.

 The pathogens that cause mastitis are classified into two categories, the so-called contagious bacteria and environmental bacteria.

 Contagious bacteria such as staphylococcus aureus and streptococcus agalactiae first colonize host tissue such as the mammary gland, milk canal, nipple lesions, etc. And they are propagated to other animals during the milking process.

 Environmental bacteria such as coliforms, streptococci, enterococci, are present in the environment where animals live, from sources such as feces, soil, beds and water, and infect udders by casual contact during the period between milking

 In all cases of bovine mastitis, whatever the causative microorganism, the route of transmission of the microorganisms to the mammary gland is through the hole of the teat and the teat canal.

 The management of milking herds focuses on two simultaneous aspects, the treatment of animals with mastitis and the prevention of new intramammary infections.

The most effective treatment to combat mastitis is treatment with antibiotics introduced as an infusion in the four quarters at the end of the period of lactation and the use of a germicidal dipping after milking during the entire lactation period.

 Dippings for tits applied after milking is the most effective procedure to reduce new intramammary infections in lactating cows.

 Bovine mastitis is an inflammation of any part of the mammary gland produced mainly by two large groups based on the origin of the bacteria: contagious pathogens and environmental pathogens. The main contagious agents are Streptococcus agalactiae, Staphylococcus aureus and several Mycoplasma species.

 The main environmental pathogens include two types of bacteria: coliform bacteria and several species of streptococci other than Strep.agalactiae These other streptococcus species are called "environmental streptococci."

 The main source of environmental pathogens is the environment where cows live, for example cows in confinement are at greater risk of environmental mastitis than grazing cows, sources of environmental pathogens include manure, beds, food, dust , The Earth and the water.

Bed materials are a significant source of exposure of environmental pathogens at the tip of the nipple. The number of bacteria in the beds fluctuates depending on the contamination (and therefore nutrient availability), humidity availability, temperature. Finely ground organic materials such as sawdust, shavings, recycled manure, husks or chopped straw often contain high streptococcus and coliform counts. Attempts to keep the number of coliforms low by applying chemical disinfectants or lime are generally impractical because they should be daily or more for positive results. Environmental conditions that may increase exposure include: overpopulation; poor ventilation: inadequate removal of manure at the rear of the dumps, corridors, feeder areas and pens, excess mud on dairy access roads, dirty maternity cubicles, etc.

 Among the main factors that influence exposure to mastitis pathogens are:

 Accommodation, animal management and nutritional management

 Size and conformation of the animal

 Pre-milking cow preparation

 SUMMARY OF THE INVENTION

 The present invention describes aqueous compositions based on copper and Aloe Vera complexes. These complexes have a marked bactericidal and fungicidal power.

DESCRIPTION OF THE FIGURES

Figure 1: Laboratory test photograph (LABSUR Laboratory, Osorno) taken on microbiological culture plates

Figure 2: Composition test photograph No. 1 on resistant S.aureus cultures. It is clear that in the areas where the product was placed there was no bacterial growth. Figure 3: Photographs showing the conditions in which the udders of approximately 40% of the herds are found.

 Figure 4: Left photograph shows an ideal state of hygiene (score 1), prior to milking, photograph on the right shows the worst state of hygiene (score 5), prior to milking.

 Figure 5: Results of the application of Copper and Aloe Vera gel in the hand of the 85-year-old Isabel Osorio Henriquez patient

 Figure 5 a: hand wound is seen before treatment

 Figure 5b: the wound is healed after 21 days.

 Figure 6: Results of the application of Copper and Aloe Vera gel in wounds of the 95-year-old Rosa Galaz patient

 Figure 6 a: Rosa Galaz before wound treatment 1

 Figure 6 b: Rosa Galaz before wound treatment 2

 Figure 6 c: Rosa Galaz wound 1 after three weeks

 Figure 6 d Rosa Galaz wound 2 after three weeks

 Figure 7: Results of the application of Copper and Aloe Vera gel in wounds of the 95-year-old Roberto Netle patient

 Figure 7 a: Roberto Netle 95 years before treatment

 Figure 7 b: Roberto Netle after 10 days of treatment

 DETAILED DESCRIPTION OF THE INVENTION

The composition includes a bactericidal and fungicidal copper complex that is prepared by forming a solution of a partially neutralized polycarboxylic acid having a molecular weight between about 1000 and 300,000, preferably a molecular weight between 2000 and 50,000 and a pH between about 3 and 8. The structure of the polycarboxylic acid is not critical in the present invention. Among the polycarboxylic acids that are useful in the present invention include partially neutralized salts of polyacrylic acid, polymethacrylic acid, copolymers of acrylic acid and methacrylic acid, copolymers of acrylic acid and acrylamide, copolymers of acrylic acid and methacrylamide, acid copolymers acrylic and methacrylate esters, copolymers of acrylic acid and maleic anhydride, carboxymethyl cellulose, copolymers of maleic acid and butadiene, polymers of maleic acid and maleic anhydride, copolymers of maleic acid and acrylic acid, copolymers of vinyl ether and maleic anhydride.

 When polycarboxylic acids are combined with water, the solution has an acidic pH.

 The partial neutralization of acidity is critical, so that the acidity is between pH 3-9, preferably between pH 3.5-5.

 In general, it has been observed that the amount of copper that can be complexed with a polycarboxylic acid is at least partly dependent on pH. The greatest amount of complexed copper is reached between the pH indicated above. Outside this pH range, the amount of copper that will complex with polycarboxylic acid is virtually useless.

 The nature of the basic compounds for neutralization of polycarboxylic acids is not critical to the composition. Sodium hydroxide, potassium hydroxide, sodium bicarbonate, sodium carbonate are included among the neutralizing agents.

The copper-containing compounds that can be used in the present invention are compounds such that when combined with partially neutralized polycarboxylic acid solutions, they provide Cu ²⁺ salts that they will complex with partially neutralized polycarboxylic acid. Among the copper compounds useful for the present invention, Cu (OH) ₂ , CuSO ₄ , Cu (CIO ₄ ) 2, Cu (NO3) 2, CuO, CuC, copper oxychloride, basic copper carbonate (Cu (OH) ₂ .CuCO ₃₎ and tribasic copper sulfate (3Cu (OH) ₂ .CuSO _4).

 The concentration of copper in the composition expressed as metallic copper may vary between 50 ppm and 10,000 ppm, preferably between 2,000 and 3,000 ppm. It is speculated that most of the copper ions (ll) are complexed and only a small part is free. Free copper is responsible for the fungicidal and bactericidal capacity of the composition.

 Another important component of the composition is aloe vera. The product used in the invention is the juice of the leaves of Aloe barbadensis which has been dried and brought to a powder state by means of a spray drying tower or freeze and vacuum drying. When this powder dissolves in water, the aloe gel is reconstituted, allowing it to be mixed together with the aqueous solution of the copper complex described above. The amounts of aloe powder present in the composition may vary from 100 ppm to 5,000 ppm preferably between 800 and 1,200 ppm expressed as 100% dry powder of Aloe Barbadensis leaf juice.

 Other components of the composition

 Although the polymer copper complex and Aloe Vera are the pillars of this composition, there are other elements that are very necessary for its good performance.

Within these elements we can point out film-forming agents, which produce improved adhesion of the composition on the skin, skin conditioning agents, thickening agents to give a consistency suitable, wetting agents that allow a better contact of the composition with the skin, dyes or pigments to color the product and one or more preservative agents.

 In order to give the composition adhesion to the skin, some type of polymer with adhesive and film-forming characteristics is used. They are included within said compounds without implying a limitation, Polyvinyl alcohol, (PVA), Polyacrylamide, polyvinylpyrrolidone (PVP), polyethylene glycols.

 The concentration of these adhesives is in the range of 0.1% and 10% by weight, preferably between 0.5% and 1%. All these elements help to produce adherent film when the product dries on the skin.

 Emollients are used as skin conditioning agents to give lubrication and protection to the skin, as conditioning elements are included without implying a limitation glycerin, Propylene Glycol and Sorbitol. Generally the range of use of these elements in the composition is 0.5% to 20% by weight, preferably in the range of 1 to 10%.

 Another necessary element of the composition is a thickening or viscosifying agent. These thickeners contribute to the formation of a protective barrier together with the adhesive compounds mentioned above. Included within said compounds without this being a limitation, xanthan gum, carboxymethyl cellulose, hydroxyethyl cellulose and natural gums. The quantity is related to giving the product the appropriate viscosity, such as to prevent the product from dripping if it is applied as dipping in the udders of cows.

Useful in the present invention are the wetting agents, these act by lowering the surface tension between the composition and the skin, allowing a better contact of the composition with the skin, thereby increasing the effectiveness of the product.

 Wetting agents that can be used in the composition are included without meaning a limitation, products such as anionic surfactants such as carboxylates, sulphonates, and alkyl sulfates, sodium dodecylbenzene sulfonate, sodium or potassium alkylsarcosinates, alpha olefin sulfonates, sulphonates with an amide or ether bond. Additionally, wetting agents such as sulfated alcohols, ethoxylated sulfated alcohols, ethoxylated alkylphenols, sulfated natural oils and fats and wetting agents such as sodium dioctylsulfoccinate are included.

 A dye or pigment can also be incorporated into the composition, as a visual aid that the product has been applied, as is the case when applied as a post dipping.

The dyes or pigments used in the composition may be any organic dye or organic or inorganic pigment which are chemically acceptable as traces in the composition. Dyes that are useful in the composition include FD&C Yellow N ^< 5 and 6, and F D&C blue N ° 1. Although a large number of dyes can be used, those dyes that have relative acceptability in liquid systems and food solids are preferred. Generally the dosage of these dyes in the composition is in the range of 0.001% to 0.01%.

As a preservative, sodium benzoate and lactic acid were used, without constituting a limitation, the dosages are between 0.1% and 3.0% Derived from the studies that were done in these formulations, another product was developed based on a copper complex with lactic acid for the purpose of using it as a milking pre-dipping agent. The purpose of pre-dipping is to wash and sanitize in a short time, udders before milking.

 It is important that the product to be used contains elements that help wash udders and also products that are effective in eliminating bacteria in short times. Very good results were obtained by adding a copper salt to form a lactic acid copper complex to a traditional pre-dipping product based on surfactants and lactic acid. Other alpha hydroxy acids can be used in this invention such as glycolic acid, citric acid.

 Composition examples- Example Ν (Composition for use as post dippinq)

 993 grams of demineralized water was loaded into a 2 liter beaker, 1.5 grams of sodium benzoate was added with stirring. It was stirred until complete dissolution, then 187.5 g of 1 100 sodium polyacrylate (partially neutralized acrylic homopolymer (20%) of molecular weight 4,500) was added. and stirred until complete homogenization. Next, 34.5 grams of 50% sodium hydroxide was added to leave the pH at a value between 4.5-4.7.

With continuous stirring, 18 grams of copper sulfate pentahydrate (CuSO ₄ x 5H ₂ O) was added.

 Stirring was continued for 1 hour to allow time for the formation of the copper complex.

Subsequently, 9 grams of food grade xanthan gum was added, which was previously dispersed in 120 grams of glycerin. Stirring continued for an hour. At this stage the solution acquires viscosity.

 Then a solution prepared by diluting 1.5 grams of 100% Aloe barbadensis Miller extract in 15 grams of demineralized water was added. The mixture was stirred for 15 minutes.

 Then 75 grams of a solution of polyvinyl alcohol (Celvol was added

325)

 At 10% concentration. It was stirred for 15 minutes.

 Subsequently, 30 grams of sodium caprilsulfonate with 38% of active ingredients were added. The mixture was stirred for an additional 15 minutes.

 Finally, a solution of 96 mgr of FD&C No. 1 dye dissolved in demineralized water was added, stirring was continued for an additional 30 minutes.

 To this preparation the copper content was measured by the atomic absorption spectroscopy method and gave a copper content of 0.29% (2,900 ppm), a refractive index of 16¾rix, a pH of 4.7 and a viscosity 800 cps with spin n ° 3 and 50 rpm (Brookfield viscometer)

 To this formulation the germicidal power was measured as described below and was also used in the field tests, also described below.

 Evaluation No. 1

 Exams with Microbiological Crops in Petri dishes

 From the beginning it was tried to determine the germicidal potential of the copper product on the most common bacterial agents in the mastitis-generating dairy farms.

While the studies were being carried out at the UACH (Universidad Austral de Chile), protocolized under international regulations (National mastitis council), preliminary field tests were carried out. Many dairies send to the laboratories milk samples for microbiological analysis, thus determining the infectious agent, in some of these samples the site determines that it is necessary to know what type of antibiotic is the most recommended by the type of agent, for this a antibiogram in which bacterial cultures are exposed to sensi-discs with antibiotics and depending on the type of growth that the bacteria have in the sensi-disc diameters will be the resistance or sensitivity to this antibiotic.

 Many of the milk samples with Staphylococcus aureus are resistant to antibiotics, in these plates we tested the Copper product with Aloe Vera.

 The tests were performed SURVET laboratory and LABSUR Laboratory, both veterinary laboratories, located in the city of Osorno, Chile.

 Survet laboratory

We realized that the product, being of gel consistency, did not penetrate homogeneously in the agar (culture medium), for which we diluted the gel solution with 2 ce of distilled water and performed the tests, which are explained with the photograph (LABSUR Laboratory, Osorno) of figure 1. In said figure, A is the plate with diluted product and B is the plate with undiluted product On the plate with the diluted product, the result for S.aureus significantly improved, inhibiting its growth in its entirety in the areas where the product was.

 The plate with the undiluted product does not penetrate the agar in a good way, lowering the effectiveness of the study, with a slight bacterial growth of S. aureus

 Conclusions

 The preliminary study carried out on microbiological culture plates helped us to know the possible bactericidal potential of the copper product with aloe vera against recurrent breast pathogens in the southern part of Chile, resulting in a wide range of bacterididal action of S. aureus, Streptoccocos, Staphylococcus coagulase negative and E.Coli.

 While this type of test is qualitative, it gave us some evidence of the bactericidal efficacy of the composition.

 Evaluation No. 2

 The product of example No. 1 was evaluated in the following work in the

Austral University.

 EVALUATION OF THE MANAGED POWER OF A FORMATION BASED ON COPPER SALTS TO BE USED AS POST MILK DIPPING IN

DAIRY COWS

 The germicidal power of a sanitizing solution based on copper salts on the most frequent mammary pathogens of bovine mastitis was evaluated in vitro to be used as post milking dipping in dairy herds.

METHODOLOGY a) Challenge strains: the following bacterial strains were used to evaluate the germicidal power of the sanitizing product: Staphylococcus aureus ATCC 25923 and Streptococcus uberis ATCC 9927 (Pankey 1984), the two most common breast pathogens in dairy farms in southern Chile

b) Method: Germicidal power was evaluated in vitro using the technique of "Extruded Nipples", recommended by the National Mastitis Council, USA (Philpot et al, 1978). The procedure consists in obtaining nipples from cows at the slaughterhouse level, all of equal size, and without lesions of the skin and hole, which were debrided to remove excess tissue, thoroughly washed and disinfected with 70% alcohol for 30 min. and subsequently maintained at -80 ^< C until the tests are carried out. The disinfectant solution was tested separately against each breast pathogen using a series of 10 nipples treated with the sanitizing product and 10 control nipples without sanitizer per assay. Before the start of each trial, the reference strains were inoculated in TSB from which bacterial suspensions containing at least 1 x 108 cfu / mL were prepared, which were maintained at maximum cooling for one week. Before performing each test, the nipples were thawed, air dried, immersed in 70% alcohol and air dried. Subsequently the nipples (10) were submerged to a depth of 15 mm in the challenge bacterial suspension for 10 sec and subsequently allowed to drain for 5 min. The same operation was repeated with the 10 control nipples (without sanitizing solution) with the difference that the drainage time in this case was 15 min. Once the drainage time has elapsed, the sanitizing product was applied to the series of treated nipples by submerging the nipples to a depth of 30 mm, allowing them to drain for 10 min. After this drainage time, each nipple was washed by dipping the nipple for 20 sec in a pp vessel containing 10 mL of TSB, rotating the vessel to recover all the post-treatment surviving bacteria. The same procedure was followed for the 10 control nipples without sanitizing solution. From each wash solution of both treated and control nipples, a duplicate plaque count was performed to determine the number of bacteria recovered from each nipple using special culture media for each breast pathogen. To obtain the final count of bacteria recovered first, the geometric mean of the duplicate plate counts was calculated and then the geometric mean of the counts of the 10 treated nipples and the 10 control nipples used in each test was calculated. Finally, to determine the germicidal power of the sanitizing solution, the geometric mean was converted to the logarithmic form and the difference between the bacteria recovered from the control group and those recovered from the treated group was calculated.

 c) Interpretation: according to the protocols of the National Mastitis Council, USA, a disinfectant product for dipping with log reductions equal to or greater than 3 with respect to the control group is considered satisfactory and can be subsequently evaluated to determine its effectiveness in preventing intramammary neoinfections using live animals with natural or experimental exposure of breast pathogens.

 RESULTS

 The log reductions of the geometric means of the mammary pathogenic bacterial counts used are presented in Table 1.

TABLE 1 Log reductions in bacterial counts of challenge strains recovered from nipples treated with a solution of copper salts and untreated control nipples. Initial concentration of geometric mean of bacteria Geometric mean of bacteria in treated and recovered nipples of the nipples bacteria recovered from controls controls the treated nipples

CFU / mL strain Log ₁₀ cfu / mL Log ₁₀ cfu / mL Log ₁₀ Reduction Reduction challenge Log%

 Staph. 170,014,705 8.23 94,478,954 7.98 1 0.00 7.98 99.9999 aureus

Strep. 2,919,000,000 9.47 1 .000.81 1 .420 8.0 2 0.30 7.7 99.9999 uberis

CONCLUSION

The composition has excellent germicidal power against Staph.aureus and Strep.uberis.

Evaluation No. 3

Use of the composition of example Ν as an antiseptic and regenerator of post-milking tissue in dairy cattle

The composition of Example No. 1 was evaluated in order to determine direct effects on the skin of the nipples of dairy cattle. Prevalence analyzes of bacteria causing mastitis of the farms were through current and remote BTA (bacteriological test of ponds), microbiological cultures of animals positive to C.M.T. (California mastitis test), in addition to T'enT® microbiological cultures used to identify bacterial presence in the sphincter tip of the nipple. An experimental trial was carried out to determine the effectiveness of the product against bacteria such as S.aureus, E.coli and Streptococcus sp, under parameters established by NMC (national mastitis council).

Description of testing grounds

Owner and geographical area N¾e Cows Type of agent Prevalent average use time of mastitis

 in milking

 Augusto Willer Sotomayor, 400 Streptococcus 18 months Pelleco, Osorno Dysgalactiae

 Jorge Matzner, Cascada, 200 S.aureus 19 months Puerto Octay

 Cristino Nanning, Frutillar 500 Streptococcus uberis, 21 months

 streptococcus

 dysgalactiae

 José Yul, Panguipulli 250 Staphylococcus 20 months

coagulase negative Staphylococcus

 aureus

 Oscar Stange, Paillaco, 700 Streptococcus uberis, 14 months Valdivia Staphylococcus

 negative coagulase

 Tomás krausse, Trafún, 200 S.aureus, Streptococcus 5 months

 Osorno uberis, S.C.N.

 Molina Crichton Ltda., 600 S.aureus, S.C.N. 1 month

 Waterfall, Puerto Octay

Nipple condition evaluation

The evaluation of the state of the nipple tips and their skin was obtained using the qualitative evaluation system developed in the milk quality laboratory, University of Vermont, Vermont. The punctuation on the states of the nipple tip and the nipple skin status score was performed before and after the use of the product to determine the general condition of the campus, in addition to identifying the cases with alterations. All nipples with alternations are evaluated monthly. Healthy nipples prior to use are selected according to the lot they represent.

Putting of the state of the tip of the nipples

0 Tip of the nipple to this subject to physical or chemical damage, not related to the treatment, or the quarter is not in lactation.

 1 The end of the sphincter of the nipple is smooth without evidence of any irritation

2 The tip of the nipple has an embossed ring

 3 The end of the sphincter of the nipple is rough with slight cracks but no redness

 4 The end of the sphincter of the nipple is inverted with many cracks that give a flowery appearance.The tip of the nipple may have old but healed constras

5 The tip of the nipple is severely damaged and ulcerated with cons open lesions Large and / or numerous warts are found, which interfere with the nipple closure function.

Score of the state of the skin of the nipples

0 The skin of the nipple is subject to physical or chemical damage (footprint, frozen, etc.), unrelated to the treatment or the cradle is not breastfeeding

 1 The nipple skin is soft, free of scales or cracks.

 2 Nipple skin shows evidence of peeling

 3 The skin of the nipple is cracked, small warts may occur

4 The skin of the nipple is cracked. There is redness indicating inflammation. Numerous warts may appear

 5 The skin of the nipple is severely damaged or ulcerated, with open scabs or lesions. Large and / or numerous warts appear that interfere with the nipple closure function

The evaluation of the condition of the nipples and especially the diagnosis of the causes that may be affecting this condition were standardized for the last time at the second symposium of mastitis and milk quality of the NMC (National Mastitis Council and the AABP (American Association) of Bovine Practitioners) held in Vancouver in 2001. Below are certain items that will make the evaluation of the nipples of dairy cattle more clearly understood, instructions for re-evaluating the nipple evaluation

1) Evaluate them after removing the milking unit and before putting on the nipple bath (post-dipping). If it is necessary to see in more detail the changes in the nipple skin, they will also need to be examined before milking.

2) Be careful when handling the nipples.

3) Observe and record data following a pattern 4) First look at the nipples without touching them

 5) Dry the nipples with paper if dark remains remain in the hole

 6) Grasp the nipple and observe it from side to side and from the base to the tip. It is very important to do it in good light, using a head lamp if necessary.

7) At least evaluate 80 cows or 20% in herds larger than 400 animals. To ensure data confidence, evaluate a representative sample of all feeding and management groups. If the herd has less than 100 animals it is better to evaluate them all.

 8) The work is much easier using a recorder, or if possible, perform the evaluation between two people, one of which will record the data.

 Types of alterations of the nipple condition

 The following characteristics were observed in the nipples, evaluating them as described below.

 Color changes

 Scale:

 one . Normal (pink)

 2. Red or bluish (cyanotic) in part or in the entire nipple

Swelling at the base of the nipple

 Scale:

 one . Normal (no ring at the base or a little swollen)

 2. Mouth mark of visible liner or "league" mark

 3. Marked swelling or palpable and thickened ring

 To simplify categories 1 and 2 you can join in a single category:

one . NORMAL (1 +2)

2. INFLATED (3) Swelling or hardness not near the tip of the nipple

 Cows with labor edema should be excluded (with one week postpartum)

one . Normal (soft and flexible)

 2. Firm, hard or wedge-shaped very marked

Nipple hole opening

 The nipple hole should be closed in the shortest possible time. However, after removing the teat cups, in some farms it is appreciated that the nipples remain open.

 one . Closed

 2. Open (more than 2mm wide or deep)

Nipple Skin Condition

 one . Normal (soft and healthy)

 2. Dry (flaky, scaly or rough but without cracks)

 3. Open lesions (also specify the type of injury: crack, fissure or black spot)

 Vascular damage

 one . Normal

 2. Petechiae or more extensive bleeding

 It is important to see how many animals are affected, as it gives us an idea of the degree of importance of the problem.

 The incidence is lower in herds with narrow lids, low milking pressure or with removers.

Hyperkeratosis of the nipple hole

 one . Without ring (N)

2. Soft or slightly rough ring (S) 3. Rough ring (R) with some strand of keratin

 4. Very rough ring (VR), keratin needles with more than 4mm of the rg, flowery appearance of the tip of the nipple.

 Interpretation of results

 Once all the nipples or at least a significant number of them have been evaluated, it should be determined whether there are alterations or problems at the level of the herd. The frequencies from which a problematic situation is considered are shown below (Table 1).

 Table 1.

 Frequencies of problems in the condition that indicate the need for further investigation

Main causes of these alterations

Alterations When Main Causes Appear

 Short-term redness • About milking

 • Wide diameter teat cups

 • Conical or wide-body upper teat cups

 • Heavy collectors

 • High milking pressure

 • Pulse failures

 • Difference between the diameter or of the nipples and the diameter of the nipples of the flock

 Swelling of the base In the short term • High pressure in the mouth of the nipple nipple associated with wide diameter

 • About milking, especially in wide or conical teat cups

 • Nipples with a rigid and narrow mouth in relation to the nipple size

 Short-term swelling or hardness • Hardness or wedge shape due to:

near or at the tip hard nipples; nipple mounted with nipple a lot

 tension (with housings longer than the teat cup); prolonged phase D; liner failure

 At the time of opening completely.

 • Swelling in the tip: over milking; wide nipples; teat cups with the camera

 wide mouth; high pressure; insufficient rest phase (D), or pulsation phase

 (A and C)

 Opening of the hole Short term • High milking pressure

 • About milking

 • Design in the mouth of the teat cup

• Units too heavy • High-pressure mounted teat cup because it is the longest housing than the teat cup

 Bad condition of the medium term • Bad weather conditions: cold, skin nipples wind, moisture, produce peeling of

(Cracks, fissures ...) the

 skin, irritation and cracking

 • Dry mud: dry skin and it loses elasticity

 • Milking machine failures: all these cracking and cracking problems get worse

 • Nipple bath disinfectants: type and concentration also make cracks worse

 • Shortage of emollients: in these nipple baths, they also make the situation worse.

Vascular damage In the medium term • Pulsation failures associated with high

(petechiae or milking pressure and / or over milking hemorrhages more

extensive)

 Long-term hyperkeratosis • Hard weather conditions or changes

(roughness, (if not abrupt of these conditions

cornification or observe • Season of the year

Callosity at the tip none of the • Nipple tip shape

of the nipple) alterations that • Production level (high)

 appear short • Lactation status and term of delivery, little is • About milking and especially milking likely to slow

 these • Genetic influence, in the shape and size alterations of the nipple.

 are caused • Composition of the nipple bath mainly • Total time of teat cups placed with by the lower milk flow machine is 1 kg. / min

milking) • Pre-milking preparation • Time the teat cups are on

• Removers

 • The last four factors are seen

 worsened by high milking pressures,

 On milking, teat cups with rigid mouths and teats mounted with a lot of tension.

Chemical, infectious and environmental factors

It is important to consider that the disinfectant used in the nipple bath, as well as the absence of suitable emollients, can be an additional source of nipple irritation and worsening of existing lesions. One must be cautious when relating lesions of the nipples with a certain post-dipping product. The problems associated with the nipple bath are more frequently related to the drying of the skin than with ulcers and erosions. If the source of the problem is really the product, simply changing the product will solve it.

Also the sudden climatic changes, can be origin of alterations in the condition. The nipples most sensitive to these changes are those of animals with edema, and wet nipples by post-dipping or milk exposed to winds and low temperatures

Just after milking. Stopping post-dipping is not the most appropriate solution, since it can cause an increase in infections by contagious microorganisms. Withdrawal of excess product before animals go outside may be an alternative.

As for infectious factors, there are two viral problems. For On the other hand, we have the bovine Pseudopoxvirus, which produces vesicles, which when broken will produce circular lesions normally in the form of a horseshoe. Healing occurs in a maximum of two weeks. The most frequent is the appearance of vesicles in the body of the nipple that cause pain to the animal and therefore hinder milking. This will eventually cause an increase in cases of mastitis. Normally the outbreak arises at a certain time, staying for a period of time, and then disappearing. Although there can also be a slow diffusion of the disease in the herd, with a low percentage of affected animals, but maintaining this situation over time. The use of nipple bath with emollients is recommended.

 On the other hand there are also lesions due to Herpesvirus or mastitis due to herpesvirus. The range of lesions is wide, from animals without symptoms, but that become difficult to milk, passing through animals with lesions that heal quickly and animals that are going to have very severe erosive lesions, which can ulcerate much of the nipple, healing very slowly and getting to produce secondary intra breast infections. Most herds have a high number of cases at first, but as the years go by, the incidence becomes occasional. Although on some occasions, the herds have a 5 or 10% incidence year after year. Both prevention and cure of the infection is quite frustrating. The reduction of edema at birth may decrease the severity of the lesions. For wound healing, barrier sealants that protect the injury from external aggression can be used.

Other infectious lesions would include papillomas or warts, which can cause difficulty in milking management, as well as being a source of bacterial infection when they break and form nipple wounds. Also exist Bacterial infections and are of greater importance, since they are a source of new intra-mammary infections. The wounds of the skin of the nipple not cured, suppose a rapid colonization by diverse bacteria, within which the Staphylococcus aureus and the Streptococcus dysgalactiae are clearly represented. You can also find primary skin infections, which cause the appearance of small purulent pustules. This process is known as staphylococcal impetigo. (II international symposium of mamitis and milk quality of the NMC and the AABP, Vancouver 2001)

 It can be seen, under the information gathered that the existence of alterations to the nipples due to the baths of post-milking solutions are recognized in the medium term and the climatic conditions fulfill a fundamental role as favoring in the presentation of these lesions.

The climate of the areas where the product of Copper Complexes was tested in a polymeric solution of Aloe Vera is rainy temperate. Its annual temperatures are quite low and the average ranges between 9 and 12 ^< C. It rains in all months of the year, although the greatest intensity in winter, rainfall varies from 1 .800 to 2,500 mm per year (Clima de Chile, Universidad From Chile)

 Results

 Dairy machinery was checked with Pullscrip measurement system, analyzing Vacuum in collectors and pulsation ratio, in cases that were outside the established parameters their status was regularized according to international recommendations.

 The following boxes describe the data to be analyzed.

The number of injured nipples after use, not considered the injured nipples before use, but its evolution will be described. Predios No. No. N ^<of No. No. No.

 No. of cows cows nipples and nipples and nipples Nipples in analyzed with Score Score Injured Injured milking nipples State Status Within Within altered Skin Tip 1 -3 months 3-6 months

 Previous nipple nipples

 use

 Property A 100 4 4 grade 2 4 grade 2 0 0

 400 4 grade 3 4 grade 1

cows

Property B 80 5 8 grade 4 8 grade 2 0 0

 200 1 grade 3 3 grade 3

cows

 Property C 100 3 4 grade 3 8 grade 2 0 0

 500

cows

 Property D 100 3 12 grade 2 0 0

 250

cows

 Property E 140 6 4 grade 2 20 grade 2 0 0

 700

cows

 Property F 80 2 4 grade 0 4 grade 0 0 0

 200

cows

 Property G 120 4 16 grade 2 0

 600

cows

Total number of cows analyzed: 720 animals

60% of cases of alteration of the nipple tip or nipple skin, are It was due to adverse environmental conditions that dried the skin of the nipples.

30% of the cases of altercations to the tip of the nipple were due to high vacuum level or inadequate pulsation ratio.

 10% of the cases of alterations of the nipple or skin tip were due to the use of improper or irritating immersion products.

 90% of the cases were resolved between 7 to 15 days, while 10% could not be evaluated by selling these animals while the study was being carried out, that 10% were cows with S.aureus in the affected rooms, at Microbiological culture with tórulas and milk.

 To these antibiotics were performed to see the degree of antibiotic resistance which was quite high, in the same antibiogram plates of resistant strains the composition of example No. 1 on S. aureus cultures was tested (Figure 2). It is clear that in the areas where the product was placed there was no bacterial growth.

 The nipples analyzed after 7 days of use of the product described above already showed clear signs of improvement, the nipples with score 4 on the nipple tip with scabs at 15 days no longer had it and the skin felt clearly soft and supple, without discomfort for cows to touch, which was very difficult at the beginning of treatment.

 The dry nipples 100% was resolved at 7 days.

 Tissues with hyperkeratosis were lowering their grade as the treatment passed, and at 30 days they were 95% resolved.

No negative alteration or damage to the nipples was seen in the cases analyzed in the course of the analyzes, if there were damages but of traumatic origin such as lacerations with barbed wires and stomps. conclusion

 The product of example No. 1 markedly improved the damage to the nipples, produced no negative alterations in any of the nipples treated at the time of study, lowered the existing prevalence rate of S.aureus and generated a markedly superior skin conditioning to what has been commonly seen or used.

 It should be noted that nipples lacerated by cuts were immersed with the product, without generating irritation in the affected area, on the contrary their healing was faster.

 Example 2

 Copper foam for use in cleaning the nipples before milking.

 (pre-dippinq)

 In a 2-liter pp glass it was loaded with 1349 grams of demineralized water, with stirring 1.5 grams of sodium benzoate was added. It was stirred until complete dissolution. Then 15 grams of sodium lauryl sulfate was added, once dissolved 42 grams of 70% sodium lauryl ether sulfate was added, once dissolved 15 grams of 85% lactic acid was added. Subsequently, sodium citrate was added to leave the Ph in a range of 2.8-3.1. Then 75 grams of Ethanol (molasses alcohol) was added and finally 2.13 grams of hydrated penta copper sulfate (CuSO4x5H2O) was added. It was stirred until complete dissolution. At this point the solution acquired a light blue color.

This solution contains 360 ppm of copper, a refractive index of 5¾rix and a pH 3. This solution was evaluated by applying it in the form of foam on the nipples, allowed to act for 30 seconds and then the product was removed with a paper towel.

 N * evaluation

 Use of Copper Foam as a pre-milking Antiseptic in dairy cattle of the X region.

 In the X region of Chile, approximately 40% of the herds are in the situations reflected in Figure 3.

Score from 1 to 5, where 1 is the animal to the left of Figure 4 and 5 is that of the right of Figure 4 (Schreinner & Ruegg, 2005, JDS)

 Research shows that an effective preparation of the udder before milking, means a reduction in the number of bacteria present in the tip of the nipple, and reduces the risk of bacteria penetration through the sphincter and therefore of new infections. To prevent mastitis and maintain good milk quality, it is essential to milk healthy, clean and dry udders.

 Well-prepared udders can accelerate the milking process and avoid residual milk, since good preparation contributes to a greater discharge of oxytocin.

In the southern part of Chile, 80% of the properties that use pre-dipping are based on iodine, but particularly when performing the procedure, they bathe the nipple with the antiseptic solution and after 15-20 seconds they already remove it, ideally the Iodine needs a minimum of 30 seconds to generate adequate bactericidal activity. In addition, many of these properties that use this pre-milking methodology have a high amount of organic matter in the nipples and this acts directly with the iodized product lowering its effectiveness through organic interaction, being a process with little effectiveness.

 Then the presence of mud and manure in the nipples of cattle are an important point for the effectiveness of a pre-milking product and thus lower the bacterial load present in the tip of the pre-milking nipple, from this the FOAM A was tested COPPER BASE as a nipple cleansing foam.

 Procedures

-Deppinera foam generator is used (AMBIC ®)

-Fill ¾ parts of the dippinera

- Foaming softener is created in the dippinera cup

 -Let it act for a minimum of 20 sec. On the nipple so that it acts with dirt and

 -Removes with disposable paper towels.

Field Testing

 A comparison of effectiveness in the field was made with two types of solutions commonly used as pre-dipping:

 A) Iodophore 3000 ppm available iodine

 B) Chlorine dioxide (0.7% lactic acid-0.64% sodium chlorite) 2400 ppm as chlorous acid.

 C) Copper foam (360 ppm copper complexed with lactic acid).

Test Site

 Owner: Cristino Nanning

 Location: Frutillar

 No. of cows in milking: approx. 500

Environmental conditions: unfavorable in winter Test Methodology

 1) 30 animals are admitted to the milking parlor

 2) Samples are taken with T'enT® torques on the nipple tip and 5 cm of

 outline of it. 1 rear nipple of each animal (left nipple) with the precaution that these tórulas do not have contact with anything except for! Nipple analyzed, in less than 5 seconds should be inserted into the guard gel. Animals are identified in torulas.

 3) Nipples of 5 animals with SOLUTION A are submerged in the left and right nipple

 4) Nipples of 5 animals with SOLUTION B are immersed in left and right rear nipples

 5) Nipples of 5 animals with SOLUTION C are submerged in left and right nipples

 6) Leave on for a minimum of 30 sec. Each solution

7) The products are removed with ELITE ^s DISPOSABLE PAPER TOWELS, leaving no product residue or visual dirt, emphasizing the tip of the nipple.

 8) Samples are taken with T'enT® torques on the nipple tip and 5 cm of

 outline of! same. 1 rear nipple of each animal (left nipple) with the precaution that these tórulas do not have contact with anything except the analyzed nipple, in less than 5 seconds it must be inserted in the guard gel. Animals are identified in torulas.

Samples are sent to SURVET laboratory in Osorno, to introduce them in culture media and identify bacterial agents present in each sample. Terms of study

Control nipples: are the nipples in which S.C.N. sample was taken: Staphylococcus coagulase negative

Negative: there was no presence or bacterial growth to the cultures

Nipples control FOOD DIOXIDE IODINE

 COPPER CHLORINE

 Bacterium

N ° 1 HONGO HONGO

N¾ HONGO HONGO

N ° 3 HONGO HONGO

N ° 4 S.C.N.- NEGATIVE

 HONGO HONGO

N¾ S.C.N. S.C.N.

N¾ Strep.uberis NEGATIVE

No. 7 S.C.N. S.C.N.

N¾ HONGO HONGO

N¾ NEGATIVE NEGATIVE Strep.uberís

N ° 10 E.coli NEGATIVE

N ° 1 1 NEGATIVE FUNGI

No. 12 S.C.N. S.C.N.

N ° 13 NEGATIVE HONGO

N ° 14 NEGATIVE FUNGI

N ° 15 Strep. NEGATIVE Uberis

N ° 16 NEGATIVE HONGO

No. 17 S.C.N. S.C.N.

N ° 18 S.C.N. S.C.N.

N ° 19 HONGO- SCNSCN NEGATIVE N¾0 Strep.uberis NEGATIVE

 N¾1 NEGATIVE HONGO

N¾2 S.C.N. NEGATIVE

N¾3 NEGATIVE FUNGI

N¾4 NEGATIVE NEGATIVE

N¾5 E.coli NEGATIVE

N¾6 NEGATIVE FUNGI

N¾7 NEGATIVE FUNGI

N¾8 S.C.N. NEGATIVE

N¾9 S.C.N. NEGATIVE

N ° 30 HONGO HONGO

Results

 The abundant presence of fungus in the samples stands out, coinciding with the environmental conditions in said analysis site. Environmental contamination of the sample is discarded by exposure time, sampling methodology and quantity present in results.

 Bacteria present are: Staphylococcus coagulase negative, E.coli,

Streptococcus uberis.

 At the time of removing the solutions in the nipples, the ease of the copper foam to remove the organic matter present in the nipples, followed by chlorine dioxide and finally iodine, is clearly noted.

 The bactericidal power of the foam seems to be adequate or more than that its drag power is effective enough to remove the microbial remains present at the tip of the nipple, it does not seem to be easily deactivated by the exposed results.

This product is being used for more than 6 months until today (August 2013) and there is no adverse effect on the skin of cows, on the contrary the nipples feel and look softer and more flexible, according to the manipulators or milkers consider This foam is very helpful because it is easy to clean and promotes softness of the skin. Example N 3

 Product formulated to treat wounds in animals and humans

 1403 grams of demineralized water was loaded into a 2 liter beaker, with stirring 4 grams of sodium benzoate was added. It was stirred until complete dissolution, then 180 g of 1 100 sodium polyacrylate (partially neutralized acrylic homopolymer 20% molecular weight 4,500) was added, stirred until complete homogenization. Then 20.3 grams of 50% sodium hydroxide was added to leave the Ph at a value between 4.5-4.7.

 With continuous stirring, 8.46 grams of copper hydroxide with a copper content of 50% of its weight was added.

 Stirring was continued for 48 hours to allow time for the formation of the copper complex. At the end of this period no insolubles are observed.

 A solution prepared with 60 grams of demineralized water, 8 grams of 85% lactic acid and 0.17 grams of copper sulphate pentahydrate was added. The mixture was stirred for 5 minutes.

 Then a solution prepared by diluting 10 grams of 100% Aloe barbadensis Miller extract in 40 grams of demineralized water was added. The mixture was stirred for one hour.

 Subsequently, 40 grams of sodium Caprylsulfonate with 38% of active ingredients were added. The mixture was stirred for an additional 15 minutes.

 Then 1 1 grams of food grade xanthan gum was added which was previously dispersed in 40 grams of glycerin. Stirring continued for an hour.

At this stage the solution acquires viscosity. Finally, 175 grams of a 10% concentration of polyvinyl alcohol solution (Celvol 325) was added. It stirred for another hour.

 A bluish green gel was obtained with a pH of 4.47 with a refractive index of 10.8 ° brix and a viscosity of 400 cps with spin 3 and 50 rpm.

 This preparation was evaluated in its healing capacity in human wounds.

 Evaluation N

 This product was evaluated in an age group of elderly people. It is known that the healing capacity of this group is diminished and they often have wounds that take a long time to heal.

 The following use protocol was used:

1 ^or the wounds are washed with physiological serum, d ebridated if applicable. Subsequently, the Copper and Aloe Vera gel of the formulation of Example No. 3 was applied and finally a surgical gauze was applied which was fixed with self-adhesive bands. Healings were made daily or day by means of which they consisted of removing the patch with the gauze, the wounds were cleaned with physiological serum and subsequently the gel was applied again and protected again with gauze and self-adhesive bands. This procedure was applied until the end of the treatment.

 Figures 5 to 7 show photographic evidence of the results of this application.

As can be seen from the photographs, all the wounds that were treated with the composition No. 3 presented a notable scar, especially considering that these people due to their advanced age have a limited healing capacity. Bibliography

Patents

Spanish Patent 2125947 Published: 10/13/1993

US Patent 5,641, 498 Published: 09/15/1995

- Dod and co-workers (F.K. Neave, F.H. Dodd and R.G. Kingwell, 1966, "A method for the control of udder diseases".

- F.H. Dodd and F.K. Neave, 1970, "Mastitis Control", Procedures, Natural Institute Research Dayring, pgs. 21 -60

- "Acid-Anionic Surfactant Sanitizers", G. R. Dychdala, Disinfection,

Sterilization and Preservation, Second Edition, 1977, pp. 253-256 ..

- "Present concepts of Bovine Mastitis." 1996, fourth Ed. National Mastitis Council, Madison Wis.

- "Antimicrobial activity of lactic acid and copper in growth of salmonella and Escherichia Coli 0: 157: H7 in a laboratory medium in Carrot Juice ^" "Salam A. Ibrahim, Hong Yang, Chung W. Seo Food Chemistry 109 (2008) pages 137-143.

-Wound Healing, Oral & Topical Activity Of Aloe Vera Davis RH; Leitner MG; Russo JM; Byrne ME J Am Podiatr Med Assoc Vol 79, Number 11, Nov 1989, P559- 62

-Anti-lnflammatory & Wound Healing Activity Of A Growth Substance In Aloe Vera Davis RH; Donato JJ; Hartman GM; Haas RC Department Of Biomedical Sciences, Pennsylvania College Of Podiatric Medicine J Am Podiatr Med Assoc 84 (2): 77-81 1994 Feb

- Aloe Vera, Hydrocortisone, & Sterol Influence On Wound Tensile Strength & Anti-lnflammation Davis RH; JJ DiDonato; Johnson RW; Stewart CB Pennsylvania College Of Podiatric Medicine J Am Podiatr Med Assoc 84 (12): 614-21 1994 Dec

-Beneficial Effects Of Aloe In Wound Healing Heggers JP; Pelley RP; Robson MC Department Of Surgery & Graduate School Of Biomedical Sciences, University Of Texas Medical Branch Phytotherapy Research (1993) Vol 7, No. Special issue, pp. S48-S52. 10 pl. (5 col. Pl.). 17 laughed.

Effect Of Aloe Vera Gel To Healing Of Burn Wound, A Clinical & Histologic Study Visuthikosol V; Chowchuen B; Sukwanarat Y; Sriurairatana S; Boonpucknavig V Department Of Surgery, Faculty Of Medicine, Ramathibodi Hospital, Mahidol University Journal heard The Medical Association of Thailand (1995 Aug) 78 (8) 403-9 Comparative Evaluation Of Aloe Vera In The Management Of Burn Wounds In Guinea Pigs Rodriguez-Bigas M; NI cross; Suarez A Surgical Research Laboratories, University Of Puerto Rico School Of Medicine Plast Reconstr Surg 81 (3): 386-9 1988 Mar

Claims

1 CLAIMS

 1. Aqueous composition based on a complex of polymeric copper and Aloe Vera CHARACTERIZED because it comprises:

a bactericidal and fungicidal copper complex

 2. Aqueous composition according to claim 1, CHARACTERIZED in that the bactericidal and fungicidal copper complex is prepared by forming a solution of a partially neutralized polycarboxylic acid having a molecular weight between about 1,000 and 300,000, preferably a molecular weight between 2000 and 50,000 and a pH between approximately 3 and 8.

 3. Aqueous composition according to claim 1, CHARACTERIZED in that the polycarboxylic acids can be partially neutralized salts of polyacrylic acid, polymethacrylic acid, copolymers of acrylic acid and methacrylic acid, copolymers of acrylic acid and acrylamide, copolymers of acrylic acid and methacrylamide, copolymers of acrylic acid and methacrylate esters, copolymers of acrylic acid and maleic anhydride, copolymers of maleic acid and butadiene, polymers of maleic acid and maleic anhydride, copolymers of maleic acid and acrylic acid, copolymers of vinyl ether and maleic anhydride.

 4. Aqueous composition according to claim 1, CHARACTERIZED in that the neutralizing agents may be sodium hydroxide, potassium hydroxide, sodium bicarbonate, sodium carbonate, among others.

5. Aqueous composition according to claim 1, CHARACTERIZED in that the copper-containing compounds are compounds such that when combined with polycarboxylic acid solutions 2

 partially neutralized, they provide Cu ++ salts that complex with partially neutralized polycarboxylic acid, being able to be Cu (OH) 2, CuSO4, Cu (CIO4) 2, Cu (NO3) 2, CuO, CuCI2, copper oxychloride, basic carbonate copper (Cu (OH) 2.CuCO3) and tribasic copper sulfate (3Cu (OH) 2.CuSO4), among others.

 6. Aqueous composition according to claim 1, CHARACTERIZED in that the concentration of copper in the composition expressed as metallic copper can vary between 50 ppm and 10,000 ppm, preferably between 2000 and 3000 ppm.

 7. Aqueous composition according to claim 1, CHARACTERIZED in that the aloe vera used corresponds to the juice of the leaves of the Aloe barbadensis which has been dried and brought to a powder state by means of a spray drying tower or freeze and vacuum drying.

 8. Aqueous composition according to claim 7, CHARACTERIZED in that the amount of aloe powder present in the composition is between 100 ppm to 10,000 ppm preferably between 800 and 1200 ppm expressed as 100% dry powder of Aloe Barbadensis leaf juice when the composition is used as post-dipping

 9. Aqueous composition according to claim 7, CHARACTERIZED in that the amount of aloe powder present in the composition is between 100 ppm to 10,000 ppm preferably between 4,000 and 6,000 when the composition is used as wound healing whether animal or human .

10. Aqueous composition according to claim 1, CHARACTERIZED in that it further comprises film-forming agents, which produce 3

 Improved adhesion of the composition on the skin, skin conditioning agents, thickening agents to give an adequate consistency, moisturizing agents that allow a better contact of the composition with the skin, dyes or pigments to color the product and one or more preservatives .

 11. Aqueous composition according to claim 9, CHARACTERIZED in that the film-forming agents are polymers with adhesive and film-forming characteristics selected from polyvinyl alcohol, (PVA), Polyacrylamide, polyvinylpyrrolidone (PVP), polyethylene glycols, among others.

12. Aqueous composition according to claim 10, CHARACTERIZED in that the concentration of the film-forming agents is in the range of 0.1% and 10% by weight, preferably between 0.5% and 1%.

 13. Aqueous composition according to claim 10, CHARACTERIZED in that it further comprises skin conditioning agents selected from glycerin, Propylene Glycol and Sorbitol.

 14. Aqueous composition according to claim 12, CHARACTERIZED in that the skin conditioning agents are in the range of 0.5% to 20% by weight, preferably in the range of 1 to 10%.

 15. Aqueous composition according to claim 10, CHARACTERIZED in that it further comprises a thickening agent selected from natural gums such as xanthan gum, carboxymethyl cellulose, hydroxyethyl cellulose and others.

16. Aqueous composition according to claim 10, CHARACTERIZED in that it further comprises wetting agents, selected from products such as anionic surfactants such as carboxylates, 4

 sulphonates, and alkyl sulfates, sodium dodecylbenzene sulphonate, sodium or potassium alkylsarcosinates, alpha olefinsulfonates, sulphonates with an amide or ether bond, additionally sulfated alcohols, ethoxylated sulfated alcohols, ethoxylated alkylphenols, sulfated natural oils and sulfoctating agents such as sodium sulphocating agents .

 17. Aqueous composition according to claim 10, CHARACTERIZED in that it further comprises dyes or pigments which can be any organic or organic or inorganic pigment which are chemically acceptable as traces in the composition, selected from FD&C Yellow N¾ and 6, and the FD&C blue No. 1.

 18. Aqueous composition according to claim 16, CHARACTERIZED in that the dyes are in the composition in the range of 0.001% to 0.01%.

 19. Aqueous composition according to claim 10, CHARACTERIZED in that it further comprises a preservative agent such as sodium benzoate and lactic acid, in the range of 0.1% and 0.5%.

 20. Use of a complex of polymeric copper and Aloe Vera CHARACTERIZED because it serves to prepare a composition useful in the treatment of wounds.

21. Use of a complex of polymer copper and Aloe Vera CHARACTERIZED because it serves to prepare a composition useful in the prevention of mastitis in cows.

22. Use of a complex of polymeric copper and Aloe Vera CHARACTERIZED because it serves to prepare a useful composition such as Post dipping in dairy 5

 23. Use of a complex of polymeric copper and Aloe Vera CHARACTERIZED because it serves to prepare a composition useful as a wound healing.

24. Use of a complex of polymeric copper and Aloe Vera CHARACTERIZED because it serves to prepare a composition useful in the treatment of fungi in animals.

 25. Use of a copper complex with alpha hydroxy acids such as lactic acid and glycolic acid, without these being a limitation, useful as a pre-dipping when used together with anionic surfactants, ethanol or isopropanol in aqueous medium.

 26. The aqueous composition according to claim 24 may have the equivalent of metallic copper in a concentration of 100 ppm and 1 000 ppm, preferably between 300 and 400 ppm

 27. The aqueous composition according to claim 24 contains anionic surfactants such as sodium lauryl, sodium lauryl ether sulfate without these being a limitation in a concentration between 0.2% and 20%, preferably between 2 and 3% .

 28. The aqueous composition according to claim 24 contains ethanol. The alcohol concentration is between 2 and 50%, preferably between 3 and 6%. Isopropanol can be used interchangeably in the same proportion.

 29. The aqueous composition according to claim 24 is useful as a pre-dipping for cleaning and hygiene of udders before milking.