WO2015112767A3 - Novel reverse transcriptases for use in high temperature nucleic acid synthesis - Google Patents

Novel reverse transcriptases for use in high temperature nucleic acid synthesis Download PDF

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Publication number
WO2015112767A3
WO2015112767A3 PCT/US2015/012534 US2015012534W WO2015112767A3 WO 2015112767 A3 WO2015112767 A3 WO 2015112767A3 US 2015012534 W US2015012534 W US 2015012534W WO 2015112767 A3 WO2015112767 A3 WO 2015112767A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
novel reverse
reverse transcriptases
high temperature
acid synthesis
Prior art date
Application number
PCT/US2015/012534
Other languages
French (fr)
Other versions
WO2015112767A2 (en
Inventor
Jeffrey Rogers
Jason Potter
Original Assignee
Life Technologies Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corporation filed Critical Life Technologies Corporation
Priority to EP15702365.6A priority Critical patent/EP3097190A2/en
Priority to CN201580014630.7A priority patent/CN106164261A/en
Priority to JP2016548054A priority patent/JP2017503521A/en
Priority to KR1020167022831A priority patent/KR20160113177A/en
Publication of WO2015112767A2 publication Critical patent/WO2015112767A2/en
Publication of WO2015112767A3 publication Critical patent/WO2015112767A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)

Abstract

The invention provides novel reverse transcriptases (RTs) with desirable properties such as increased thermostability, increased thermoreactivity and/or increased resistance to inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cDNA molecules) using kits, compositions and/or reactions mixtures containing such novel reverse transcriptase enzymes.
PCT/US2015/012534 2014-01-22 2015-01-22 Novel reverse transcriptases for use in high temperature nucleic acid synthesis WO2015112767A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP15702365.6A EP3097190A2 (en) 2014-01-22 2015-01-22 Novel reverse transcriptases for use in high temperature nucleic acid synthesis
CN201580014630.7A CN106164261A (en) 2014-01-22 2015-01-22 It is applicable to the novel reverse transcriptase of high temperature nucleic acid synthesis
JP2016548054A JP2017503521A (en) 2014-01-22 2015-01-22 Novel reverse transcriptase for use in high temperature nucleic acid synthesis
KR1020167022831A KR20160113177A (en) 2014-01-22 2015-01-22 Novel reverse transcriptases for use in high temperature nucleic acid synthesis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461930395P 2014-01-22 2014-01-22
US61/930,395 2014-01-22

Publications (2)

Publication Number Publication Date
WO2015112767A2 WO2015112767A2 (en) 2015-07-30
WO2015112767A3 true WO2015112767A3 (en) 2015-10-22

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/012534 WO2015112767A2 (en) 2014-01-22 2015-01-22 Novel reverse transcriptases for use in high temperature nucleic acid synthesis

Country Status (6)

Country Link
US (2) US9663770B2 (en)
EP (1) EP3097190A2 (en)
JP (1) JP2017503521A (en)
KR (1) KR20160113177A (en)
CN (1) CN106164261A (en)
WO (1) WO2015112767A2 (en)

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Also Published As

Publication number Publication date
US9663770B2 (en) 2017-05-30
KR20160113177A (en) 2016-09-28
CN106164261A (en) 2016-11-23
JP2017503521A (en) 2017-02-02
EP3097190A2 (en) 2016-11-30
US20150210989A1 (en) 2015-07-30
US20180010105A1 (en) 2018-01-11
WO2015112767A2 (en) 2015-07-30

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