WO2017057917A1 - Human scalp mimic and method for measuring scalp cleansing effect by using same - Google Patents

Human scalp mimic and method for measuring scalp cleansing effect by using same Download PDF

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Publication number
WO2017057917A1
WO2017057917A1 PCT/KR2016/010903 KR2016010903W WO2017057917A1 WO 2017057917 A1 WO2017057917 A1 WO 2017057917A1 KR 2016010903 W KR2016010903 W KR 2016010903W WO 2017057917 A1 WO2017057917 A1 WO 2017057917A1
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scalp
human
mimetic
measuring
human scalp
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PCT/KR2016/010903
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French (fr)
Korean (ko)
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정인근
박상철
김부민
조선아
김지훈
배우리
김현나
김세현
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(주)아모레퍼시픽
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/10—Hair or skin implants
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L23/00—Compositions of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Compositions of derivatives of such polymers
    • C08L23/02—Compositions of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Compositions of derivatives of such polymers not modified by chemical after-treatment
    • C08L23/04—Homopolymers or copolymers of ethene
    • C08L23/06—Polyethene
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L27/00—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a halogen; Compositions of derivatives of such polymers
    • C08L27/02—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a halogen; Compositions of derivatives of such polymers not modified by chemical after-treatment
    • C08L27/04—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a halogen; Compositions of derivatives of such polymers not modified by chemical after-treatment containing chlorine atoms
    • C08L27/06—Homopolymers or copolymers of vinyl chloride
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L83/00—Compositions of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing silicon with or without sulfur, nitrogen, oxygen or carbon only; Compositions of derivatives of such polymers
    • C08L83/04—Polysiloxanes

Definitions

  • Disclosed herein is a method for measuring the scalp cleansing effect using a human scalp mimetic.
  • an object of the present invention is to provide a scalp mimic that reproduces a human scalp.
  • an object of the present invention is to provide a scalp mimetic with chemical stability.
  • an object of the present invention is to provide a method for measuring the scrubbing effect of the scalp using a scalp mimetic.
  • the invention comprises a polymer plate comprising a plurality of grooves on the surface, the plurality of grooves, the average distance between the edges of adjacent grooves located in the same column or row, 700 ⁇ m to 1500 ⁇ m,
  • the elasticity of the polymer plate is a scalp mimetic that is 300 MPa or less as measured at 23 ° C. according to ISO 27 (2011) certified by the International Organization for Standardization.
  • the present invention comprises the steps of applying a hair care material to the scalp mimetic.
  • the scalp mimetic which is an aspect of the present invention, reflects the structure of human scalp, and thus may be usefully used in scalp related experiments. Specifically, since the spacing of the space, the depth of the pores and the distance between the pores and the hair is similar to the actual human scalp, more accurate experimental results can be obtained. In addition, the production cost is reduced and economical, and it is free from ethical issues because it does not require the use of animal scalp.
  • 1 is a schematic representation of a human scalp mimetic.
  • Figure 2 is a diagram of the human scalp mimetic observation with an electron microscope.
  • FIG. 3 shows a photomask used to prepare a human scalp mimetic.
  • Figure 4 is a photograph confirming the presence of coacervate in the pores of the human scalp mimetic.
  • the present invention includes a polymer plate comprising a plurality of grooves on the surface, the plurality of grooves, the average distance between the edge of the adjacent grooves is 700 ⁇ m to 1500 ⁇ m, the elasticity of the polymer plate is Human scalp mimetic, measured at 23 ° C. according to ISO 37 (2011), certified by the International Organization for Standardization.
  • ISO 37 (2011) is a method for measuring the tensile stress-strain characteristics of thermoplastic rubbers, certified by the International Organization for Standardization.
  • the test conditions of ISO 37 (2011) follow ISO 23529 (Rubber-General procedures for preparing and conditioning test pieces for physical test methods, 2010) certified by the International Organization for Standardization, and humidity conditions are not considered.
  • the measurement temperature can be 23 ⁇ 2 ° C or 27 ⁇ 2 ° C.
  • Preparation of the scalp mimetic sample for elasticity measurement is performed 3 hours before the elasticity measurement.
  • the human scalp mimetic may refer to an artificial scalp that reflects physical characteristics of an actual human scalp.
  • human scalp mimetics are similar to those of real human scalp, such as the density of artificial pores in the scalp mimetics or the distance of artificial hair space, the distance between artificial pores and artificial hairs present in artificial pores, diameter and elasticity of artificial pores. can do.
  • the human scalp mimetic may also reflect the electrical characteristics of the actual human scalp.
  • human scalp mimetics may contain some negative charges, including hydroxyl groups on their surface.
  • the polymer plate may be a flat plate made of a polymer material, but may be modified in shape depending on the intended use.
  • the plurality of grooves may have an average distance between edges of adjacent grooves located in the same column or row, and may be 700 ⁇ m to 1500 ⁇ m, and 700 to 1400 ⁇ m, 800 to 1400 ⁇ m, 900 to 1400 ⁇ m, 700 To 1300 ⁇ m, 700 to 1200 ⁇ m, 800 to 1300 ⁇ m, 800 to 1200 ⁇ m, 900 to 1100 ⁇ m, but are not limited thereto.
  • the average distance between edges of adjacent grooves may mean a value obtained by dividing the measured distance between individual adjacent grooves by the number of pairs of adjacent grooves measuring the distance.
  • the groove on the surface of the polymer plate may mean a portion dug to a certain depth on the surface, and in one embodiment, the groove may be cylindrical.
  • the polymer plate may have an elasticity of less than 300MPa.
  • the elasticity of the polymer plate can be measured by a known elasticity measuring method.
  • the elasticity is 10 to 300 MPa, 10 to 290 MPa, 10 to 270 MPa, 10 to 250 MPa, 10 to 230 MPa, 10 to 210 MPa, 10 to 190 MPa, 10 to 170 MPa, 10 to 150 MPa, 10 to 130 MPa, 10 to 110 MPa, 10 to 10 90 MPa, 10 to 70 MPa, 10 to 50 MPa, 10 to 30 MPa, 30 to 300 MPa, 50 to 250 MPa, 80 to 200 MPa, or 120 to 150 MPa, but is not limited thereto.
  • the polymer plate may include a protrusion located inside the groove in a state spaced apart from the inner wall of the groove.
  • the protrusion may refer to the reproduction of the hair on the actual human scalp, and the length or height thereof is not limited. In one embodiment, the height (length) of the protrusion may be equal to the depth of the groove.
  • the average distance between the inner wall of the groove and the outer wall of the protrusion may be 30 ⁇ m to 60 ⁇ m.
  • the average distance between the inner wall of the groove and the outer wall of the protrusion may mean an average value of the shortest distance and the longest distance between the outer wall of the protrusion and the inner wall of the groove in each groove.
  • the groove and the protrusion may be cylindrical, each center may coincide.
  • the average distance between the inner wall of the groove and the outermost part of the protrusion is 30 ⁇ m, 40 ⁇ m, 41 ⁇ m, 42 ⁇ m, 43 ⁇ m, 44 ⁇ m, 45 ⁇ m, 46 ⁇ m, 47 ⁇ m, 48 ⁇ m, 49 ⁇ m, 50 ⁇ m, 55 ⁇ m or 60 ⁇ m, but is not limited thereto.
  • the depth of the groove may be 370 ⁇ m or less.
  • the depth of the groove may mean a vertical distance from the virtual horizon of the surface of the polymer plate to the bottom of the groove.
  • the depth is 60 ⁇ m to 370 ⁇ m, 60 ⁇ m to 360 ⁇ m, 60 ⁇ m to 350 ⁇ m, 70 ⁇ m to 370 ⁇ m, 80 ⁇ m to 370 ⁇ m, 90 ⁇ m to 360 ⁇ m, 90 ⁇ m to 350 ⁇ m, 120 ⁇ m to 370 ⁇ m , 150 ⁇ m to 340 ⁇ m, 180 ⁇ m to 300 ⁇ m, 200 ⁇ m to 250 ⁇ m, 60 ⁇ m to 90 ⁇ m, or 60 ⁇ m to 80 ⁇ m, but is not limited thereto.
  • the polymer may be, but is not limited to, polydimethylsiloxane (PDMS), low density polyethylene (LDPE) or polyvinyl chloride (polyvinyl chloride).
  • PDMS polydimethylsiloxane
  • LDPE low density polyethylene
  • polyvinyl chloride polyvinyl chloride
  • the human scalp mimetic may be made of polydimethylsiloxane, which may be easy for precise analysis because elasticity is similar to real human scalp and little chemical reactivity.
  • the thickness of the polymer plate may be 1mm to 4mm.
  • the thickness may be 1 mm to 4 mm, 1.1 mm to 3.9 mm, 1.5 mm to 3.5 mm, 1.8 mm to 3.3 mm, 2.0 mm to 3.1 mm, 2.0 mm to 3.0 mm, but is not limited thereto.
  • the polymer plate may comprise a hydroxyl group on the surface.
  • the human scalp is generally neutral, but partially negatively charged, including hydroxyl groups on the polymer plate, to reproduce the electrical and chemical properties, such as hydrophilicity, of the human scalp.
  • the hydroxyl group may be attached onto the polymer plate by plasma etching, but is not limited thereto and may attach the hydroxyl group by a known surface treatment method.
  • the present invention may relate to the use of a human scalp mimetic for measuring scalp cleansing effects.
  • the invention may relate to a human scalp mimetic for artificial scalp experiments.
  • the present invention may relate to a human scalp mimetic for measuring scalp cleansing effect.
  • the step of applying a hair care material to the scalp mimetic; And washing the scalp after application may further comprise measuring the amount of material change on the scalp before and after scalp cleaning.
  • the cleaning may mean not only removing the scalp contaminants from the scalp, but also removing the hair cleansing agent composition used to remove the scalp contaminants from the scalp. Cleaning can be performed using only water, and can also be performed using a cleaning material composition.
  • the contaminants may include fine dust, dandruff and skin secretions.
  • the hair care material may be a hair cleaning composition or hair cosmetic composition.
  • the hair care material may be a generic name for materials used for cleaning, nourishing, or cosmetology of the scalp or hair.
  • the hair cleansing composition may include a shampoo or rinse
  • the hair cosmetic composition may include a hair tonic, a hair dressing agent such as a gel or a mousse, a wool or a hair dye, and the like.
  • a photomask suitable for a 6 inch silicon wafer was prepared according to the scalp pattern (FIG. 3).
  • the spacing between the hair was set to 1000 mu m and the thickness of the hair was 80 mu m.
  • a photoresist (Photoregistive, JSR 151-N manufacturer JSR, Japan) was coated on the 6-inch silicon wafer to the same thickness as the depth of the pores. Thereafter, the photomask was attached onto the silicon wafer coated with the photosensitive agent, and then irradiated with UV to harden only the hair and the pores of the pattern, and the outer parts of the hair and the pores were removed with an organic solution. Thereafter, the surface of the silicon wafer was again coated with a metal or an inorganic coating material using a vacuum deposition apparatus, and the photoresist was removed with an organic solution.
  • the polydimethylsiloxane solution (manufacturer; Dow Corning) dissolved on the surface of the silicon wafer inscribed with the scalp pattern was engraved and hardened at room temperature. The hardened polydimethylsiloxane was removed from the silicon wafer to finally produce a scalp mimetic.
  • the cationic polymer used was polyquaternium-10 (sigma aldrich) and the anionic polymer was sodium laureth sulfate (sigma aldrich). Thereafter, the mixed solution was discarded, and purified water was placed on the scalp mimic surface and washed. Thereafter, the coacervate remaining in the artificial pores was identified using a confocal laser scanning microscope (Confocal Laser Scanning Microsocpy) to confirm the coacervate formed in the pores (FIG. 4).

Abstract

Disclosed in the present specification are a human scalp mimic and a method for measuring a scalp cleansing effect by using the same. The human scalp mimic, which is one aspect of the present invention, reflects the scalp structure of a person, thereby being useful in experiments related to the scalp. Particularly, the gap between pores, the depth of pores, and the distance between pores and the hair are similar to those of the scalp of a real person, and thus a more accurate experimental result can be obtained. In addition, the present invention is economical since manufacturing costs are reduced and is free from ethical issues since the scalp of an animal does not need to be used.

Description

인간 두피 모사체와 이를 이용한 두피 세정효과 측정 방법Human scalp mimetics and methods for measuring scalp cleaning effects
본 명세서에는 인간의 두피 모사체와 이를 이용한 두피 세정효과의 측정 방법이 개시된다.Disclosed herein is a method for measuring the scalp cleansing effect using a human scalp mimetic.
현재 국내 인구의 약 25% 정도가 두피 질환을 경험하고 있으며, 두피 질환의 원인은 다양하나, 크게 오염물질 등의 외인성 요인에 의한 것과 유전 및 스트레스 등의 내인성 요인으로 나뉘며, 그 발생 형태 역시 건선, 탈모 등으로 다양하다. 특히 최근에는 탈모 인구가 점차 증가하고, 특히 20 ~ 30대의 탈모 환자도 증가하고 있으며, 여성 탈모인구 또한 급격하게 증가하고 있는 추세이다. 이러한 추이는 세계적으로도 유사하여, 육류 섭취가 많은 유럽과 미국을 중심으로 두피 질환 환자의 증가 폭이 큰 것으로 나타나고 있다. 따라서, 두피 질환의 치료 중요성은 매우 높아지고 있으며, 관련 치료기술 및 진단기술의 개발이 절실한 실정이다.Currently, about 25% of the Korean population experiences scalp disease, and the causes of scalp disease vary widely, but are divided into exogenous factors such as pollutants and endogenous factors such as heredity and stress. It is various, such as hair loss. In particular, in recent years, the hair loss population is gradually increasing, especially the hair loss patients in their 20s and 30s, and the female hair loss population is also increasing rapidly. This trend is similar in the world, and the number of patients with scalp disease is increasing, especially in Europe and the United States, where meat consumption is high. Therefore, the importance of the treatment of scalp disease is very high, the development of related treatment techniques and diagnostic techniques are urgently needed.
최근 두피 건강을 향상시키기 위하여, 건강 보조식품, 헤어 세정 용품 등의 연구가 활발하다. 그러나, 인간 두피 환경을 반영한 인공 두피의 개발은 미미하고, 생체 시료를 이용한 실험이 진행 중이나, 이는 윤리 문제가 뒤따르며, 실험의 정밀성이 떨어진다. 그 결과, 현재 두피 건강에 대한 연구는 한계가 있다.Recently, in order to improve scalp health, research on health supplements, hair cleaning products, and the like is active. However, the development of artificial scalp reflecting the human scalp environment is insignificant, and experiments using biological samples are in progress, but this is accompanied by ethical issues, and the precision of the experiment is poor. As a result, current research on scalp health is limited.
일 측면에서 본 발명의 목적은, 사람의 두피를 재연한 두피 모사체를 제공하는 것이다.In one aspect, an object of the present invention is to provide a scalp mimic that reproduces a human scalp.
다른 측면에서 본 발명의 목적은, 화학적 안정성이 있는 두피 모사체를 제공하는 것이다.In another aspect, an object of the present invention is to provide a scalp mimetic with chemical stability.
다른 측면에서 본 발명의 목적은, 두피 모사체를 이용하여, 두피의 세정효과를 측정하는 방법을 제공하는 것이다.In another aspect, an object of the present invention is to provide a method for measuring the scrubbing effect of the scalp using a scalp mimetic.
일 측면에서, 본 발명은, 표면상에 복수 개의 홈들을 포함하는 고분자 플레이트를 포함하고, 상기 복수 개의 홈들은, 동일한 열 또는 행에 위치한 인접 홈의 가장자리 간의 평균 거리가 700㎛ 내지 1500㎛이고, 상기 고분자 플레이트의 탄성은, 국제 표준화 기구에 의해 인증된 ISO 27(2011)에 따라 23℃에서 측정하였을 때 300MPa 이하인, 두피 모사체이다. In one aspect, the invention comprises a polymer plate comprising a plurality of grooves on the surface, the plurality of grooves, the average distance between the edges of adjacent grooves located in the same column or row, 700㎛ to 1500㎛, The elasticity of the polymer plate is a scalp mimetic that is 300 MPa or less as measured at 23 ° C. according to ISO 27 (2011) certified by the International Organization for Standardization.
다른 측면에서, 본 발명은, 두피 모사체에 헤어 케어용 물질을 도포하는 단계; 및In another aspect, the present invention comprises the steps of applying a hair care material to the scalp mimetic; And
도포 후 두피를 세정하는 단계를 포함하는, 두피 세정효과 측정 방법이다.It is a method of measuring the scalp cleaning effect, comprising the step of washing the scalp after application.
본 발명의 일 측면인 두피 모사체는, 사람의 두피의 구조를 반영하였으므로, 두피 관련 실험에서 유용하게 사용할 수 있다. 구체적으로, 모공간의 간격, 모공의 깊이 및 모공과 모발간의 거리가 실제 사람 두피와 유사하므로, 보다 정확한 실험 결과를 얻을 수 있다. 또한, 제작 비용이 절감되어 경제적이고, 동물의 두피를 사용하지 않아도 되기 때문에, 윤리적 문제로부터 자유롭다.The scalp mimetic, which is an aspect of the present invention, reflects the structure of human scalp, and thus may be usefully used in scalp related experiments. Specifically, since the spacing of the space, the depth of the pores and the distance between the pores and the hair is similar to the actual human scalp, more accurate experimental results can be obtained. In addition, the production cost is reduced and economical, and it is free from ethical issues because it does not require the use of animal scalp.
도 1은 인간 두피 모사체의 개략도이다.1 is a schematic representation of a human scalp mimetic.
도 2는 인간 두피 모사체를 전자 현미경으로 관찰한 도이다.Figure 2 is a diagram of the human scalp mimetic observation with an electron microscope.
도 3은 인간 두피 모사체를 제조하는데 사용된 포토마스크를 보이는 도이다.3 shows a photomask used to prepare a human scalp mimetic.
도 4는 인간 두피 모사체의 모공 내 코아세르베이트의 존재를 확인한 사진이다.Figure 4 is a photograph confirming the presence of coacervate in the pores of the human scalp mimetic.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
일 측면에서, 본 발명은, 표면상에 복수 개의 홈들을 포함하는 고분자 플레이트를 포함하고, 상기 복수 개의 홈들은, 인접 홈들의 가장자리 간의 평균 거리가 700㎛ 내지 1500㎛이고, 상기 고분자 플레이트의 탄성은, 국제 표준화 기구에 의해 인증된 ISO 37(2011)에 따라 23℃에서 측정하였을 때, 300MPa 이하인, 인간 두피 모사체이다.In one aspect, the present invention includes a polymer plate comprising a plurality of grooves on the surface, the plurality of grooves, the average distance between the edge of the adjacent grooves is 700㎛ to 1500㎛, the elasticity of the polymer plate is Human scalp mimetic, measured at 23 ° C. according to ISO 37 (2011), certified by the International Organization for Standardization.
상기 ISO 37(2011)은, 국제 표준화 기구(International Organization for Standardization)에 의해 인증 받은, 열가소성 고무의 인장 응력-변형 특성을 측정하기 위한 방법이다. 상기 ISO 37(2011)의 실험 조건은 국제 표준화기구에 의해 인증 받은 ISO 23529(Rubber-General procedures for preparing and conditioning test pieces for physical test methods, 2010)를 따르며, 습도 조건은 고려하지 않는다. 측정 온도는 23 ± 2℃ 또는 27 ± 2℃일 수 있다. 탄성 측정을 위한 두피모사체 샘플의 준비는 탄성 측정 3시간 이전에 한다.ISO 37 (2011) is a method for measuring the tensile stress-strain characteristics of thermoplastic rubbers, certified by the International Organization for Standardization. The test conditions of ISO 37 (2011) follow ISO 23529 (Rubber-General procedures for preparing and conditioning test pieces for physical test methods, 2010) certified by the International Organization for Standardization, and humidity conditions are not considered. The measurement temperature can be 23 ± 2 ° C or 27 ± 2 ° C. Preparation of the scalp mimetic sample for elasticity measurement is performed 3 hours before the elasticity measurement.
본 명세서에서 인간 두피 모사체란, 실제 인간 두피의 물리적 특성을 반영한 인공 두피를 의미할 수 있다. 예컨대, 인간 두피 모사체는, 두피 모사체 내의 인공 모공의 밀도 또는 인공 모공간의 거리, 인공 모공과 인공 모공 내 존재하는 인공 모발간의 거리, 인공 모공의 지름 및 탄성 등이 실제 인간 두피의 그것들과 유사할 수 있다. 또한, 인간 두피 모사체에는 실제 인간 두피의 전기적 특성도 반영되어 있을 수 있다. 예컨대, 인간 두피 모사체는 표면에 하이드록시기를 포함하여 일부 음성 전하를 포함할 수 있다.As used herein, the human scalp mimetic may refer to an artificial scalp that reflects physical characteristics of an actual human scalp. For example, human scalp mimetics are similar to those of real human scalp, such as the density of artificial pores in the scalp mimetics or the distance of artificial hair space, the distance between artificial pores and artificial hairs present in artificial pores, diameter and elasticity of artificial pores. can do. In addition, the human scalp mimetic may also reflect the electrical characteristics of the actual human scalp. For example, human scalp mimetics may contain some negative charges, including hydroxyl groups on their surface.
본 명세서에서, 고분자 플레이트는 고분자 물질로 만들어진 평판일 수 있으나, 사용 목적에 따라서 모양을 변형할 수 있다.In the present specification, the polymer plate may be a flat plate made of a polymer material, but may be modified in shape depending on the intended use.
상기와 같은 관점에서, 상기 복수개의 홈은 동일한 열 또는 행에 위치한 인접 홈의 가장자리 간의 평균 거리가 700㎛ 내지 1500㎛일 수 있고, 700 내지 1400㎛, 800 내지 1400㎛, 900 내지 1400㎛, 700 내지 1300㎛, 700 내지 1200㎛, 800 내지 1300㎛, 800 내지 1200㎛, 900 내지 1100㎛ 일 수 있으나 이에 제한되는 것은 아니다. In view of the above, the plurality of grooves may have an average distance between edges of adjacent grooves located in the same column or row, and may be 700 μm to 1500 μm, and 700 to 1400 μm, 800 to 1400 μm, 900 to 1400 μm, 700 To 1300 μm, 700 to 1200 μm, 800 to 1300 μm, 800 to 1200 μm, 900 to 1100 μm, but are not limited thereto.
본 명세서에서, 인접 홈의 가장자리 간의 평균 거리란, 측정된 개개의 인접 홈 간의 거리의 합을, 거리를 측정한 인접 홈의 쌍의 수로 나눈 값을 의미할 수 있다. In the present specification, the average distance between edges of adjacent grooves may mean a value obtained by dividing the measured distance between individual adjacent grooves by the number of pairs of adjacent grooves measuring the distance.
본 명세서에서 고분자 플레이트 표면상의 홈이란, 표면상에 일정 깊이로 파인 부분을 의미할 수 있으며, 일 구현예에서, 홈은 원통 모양일 수 있다.In the present specification, the groove on the surface of the polymer plate may mean a portion dug to a certain depth on the surface, and in one embodiment, the groove may be cylindrical.
또한, 상기와 같은 측면에서, 상기 고분자 플레이트는, 300MPa 이하의 탄성을 가질 수 있다. 고분자 플레이트의 탄성은 공지의 탄성 측정 방법에 의하여 측정될 수 있다. 상기 탄성은, 10 내지 300MPa, 10 내지 290MPa, 10 내지 270MPa, 10 내지 250MPa, 10 내지 230MPa, 10 내지 210MPa, 10 내지 190MPa, 10 내지 170MPa, 10 내지 150MPa, 10 내지 130MPa, 10 내지 110MPa, 10 내지 90MPa, 10 내지 70MPa, 10 내지 50MPa, 10 내지 30MPa, 30 내지 300MPa, 50 내지 250MPa, 80 내지 200MPa, 또는 120 내지 150MPa일 수 있으나, 이에 제한되는 것은 아니다. In addition, in the above aspect, the polymer plate may have an elasticity of less than 300MPa. The elasticity of the polymer plate can be measured by a known elasticity measuring method. The elasticity is 10 to 300 MPa, 10 to 290 MPa, 10 to 270 MPa, 10 to 250 MPa, 10 to 230 MPa, 10 to 210 MPa, 10 to 190 MPa, 10 to 170 MPa, 10 to 150 MPa, 10 to 130 MPa, 10 to 110 MPa, 10 to 10 90 MPa, 10 to 70 MPa, 10 to 50 MPa, 10 to 30 MPa, 30 to 300 MPa, 50 to 250 MPa, 80 to 200 MPa, or 120 to 150 MPa, but is not limited thereto.
상기와 같은 측면에서, 상기 고분자 플레이트는, 상기 홈의 내벽과 이격된 상태로 홈의 내부에 위치하는 돌출부를 포함할 수 있다. In the above aspect, the polymer plate may include a protrusion located inside the groove in a state spaced apart from the inner wall of the groove.
본 명세서에서, 돌출부란, 실제 인간 두피 상의 모발을 재연한 것을 의미할 수 있고, 그 길이 또는 높이는 제한되지 않는다. 일 구현예에서, 상기 돌출부의 높이(길이)는 상기 홈의 깊이와 동일할 수 있다. In the present specification, the protrusion may refer to the reproduction of the hair on the actual human scalp, and the length or height thereof is not limited. In one embodiment, the height (length) of the protrusion may be equal to the depth of the groove.
상기와 같은 측면에서, 상기 홈의 내벽과 돌출부의 외벽 사이의 평균 거리는 30㎛ 내지 60㎛일 수 있다. In the above aspect, the average distance between the inner wall of the groove and the outer wall of the protrusion may be 30㎛ to 60㎛.
본 명세서에서 홈의 내벽과 돌출부의 외벽 사이의 평균 거리란, 개개의 홈에서 돌출부의 외벽과 그 홈의 내벽간의 최단 거리와 최장 거리의 평균값을 의미할 수 있다. 또한 일 구현예에서, 상기 홈과 상기 돌출부는 원통 모양일 수 있고, 각각의 중심이 일치할 수 있다. In this specification, the average distance between the inner wall of the groove and the outer wall of the protrusion may mean an average value of the shortest distance and the longest distance between the outer wall of the protrusion and the inner wall of the groove in each groove. In addition, in one embodiment, the groove and the protrusion may be cylindrical, each center may coincide.
상기 홈의 내벽과 돌출부의 최외각 사이의 평균 거리는, 30㎛, 40㎛, 41㎛, 42㎛, 43㎛, 44㎛, 45㎛, 46㎛, 47㎛, 48㎛, 49㎛, 50㎛, 55㎛ 또는 60㎛일 수 있으나, 이에 제한되는 것은 아니다.The average distance between the inner wall of the groove and the outermost part of the protrusion is 30 μm, 40 μm, 41 μm, 42 μm, 43 μm, 44 μm, 45 μm, 46 μm, 47 μm, 48 μm, 49 μm, 50 μm, 55 μm or 60 μm, but is not limited thereto.
또한, 상기와 같은 측면에서, 상기 홈의 깊이는 370㎛ 이하일 수 있다. 홈의 깊이란, 상기 고분자 플레이트 표면의 가상의 지평선으로부터 홈의 바닥까지의 수직 거리를 의미할 수 있다. 상기 깊이는 60㎛ 내지 370㎛, 60㎛ 내지 360㎛, 60㎛ 내지 350㎛, 70㎛ 내지 370㎛, 80㎛ 내지 370㎛, 90㎛ 내지 360㎛, 90㎛ 내지 350㎛, 120㎛ 내지 370㎛, 150㎛ 내지 340㎛, 180㎛ 내지 300㎛, 200㎛ 내지 250㎛, 60㎛ 내지 90㎛, 또는 60㎛ 내지 80㎛일 수 있으나, 이에 제한되는 것은 아니다.In addition, in the above aspect, the depth of the groove may be 370㎛ or less. The depth of the groove may mean a vertical distance from the virtual horizon of the surface of the polymer plate to the bottom of the groove. The depth is 60 μm to 370 μm, 60 μm to 360 μm, 60 μm to 350 μm, 70 μm to 370 μm, 80 μm to 370 μm, 90 μm to 360 μm, 90 μm to 350 μm, 120 μm to 370 μm , 150 μm to 340 μm, 180 μm to 300 μm, 200 μm to 250 μm, 60 μm to 90 μm, or 60 μm to 80 μm, but is not limited thereto.
상기와 같은 측면에서, 상기 고분자는 폴리디메틸실록산(polydimethylsiloxane, PDMS), 저밀도 폴리에틸렌(low density polyethylene, LDPE) 또는 폴리비닐클로라이드 (polyvinyl chloride)일 수 있으나, 이에 제한되지 않는다.In the above aspect, the polymer may be, but is not limited to, polydimethylsiloxane (PDMS), low density polyethylene (LDPE) or polyvinyl chloride (polyvinyl chloride).
일 구현예에서 상기 인간 두피 모사체는 폴리디메틸실록산으로 제조될 수 있는데, 이러한 두피 모사체는 탄성이 실제 인간 두피와 유사하고, 화학 반응성이 거의 없으므로, 정밀 분석에 용이할 수 있다.In one embodiment, the human scalp mimetic may be made of polydimethylsiloxane, which may be easy for precise analysis because elasticity is similar to real human scalp and little chemical reactivity.
상기와 같은 측면에서, 상기 고분자 플레이트의 두께는, 1mm 내지 4mm일 수 있다. 상기 두께는, 1mm 내지 4mm, 1.1mm 내지 3.9mm, 1.5mm 내지 3.5mm, 1.8mm 내지 3.3mm, 2.0mm 내지 3.1mm, 2.0mm 내지 3.0mm일 수 있으나, 이에 제한되는 것은 아니다.In the above aspect, the thickness of the polymer plate may be 1mm to 4mm. The thickness may be 1 mm to 4 mm, 1.1 mm to 3.9 mm, 1.5 mm to 3.5 mm, 1.8 mm to 3.3 mm, 2.0 mm to 3.1 mm, 2.0 mm to 3.0 mm, but is not limited thereto.
일 구현예에서, 상기 고분자 플레이트는 표면상에 수산화기를 포함할 수 있다. 일반적으로, 인간 두피는 전체적으로는 중성이지만, 일부분 음전하를 띄고 있는데, 상기 고분자 플레이트 상에 수산화기를 포함하여, 상기와 같은 인간 두피의 전기적 특성과 화학적 특성, 예컨대 친수성을 재연할 수 있다. In one embodiment, the polymer plate may comprise a hydroxyl group on the surface. In general, the human scalp is generally neutral, but partially negatively charged, including hydroxyl groups on the polymer plate, to reproduce the electrical and chemical properties, such as hydrophilicity, of the human scalp.
상기 수산화기는 플라즈마 에칭법에 의하여 고분자 플레이트 상에 부착될 수 있으나, 제한되지 않고, 공지의 표면 처리법에 의하여 수산화기를 부착시킬 수 있다.The hydroxyl group may be attached onto the polymer plate by plasma etching, but is not limited thereto and may attach the hydroxyl group by a known surface treatment method.
본 발명은 다른 관점에서, 두피 세정 효과를 측정하기 위한 인간 두피 모사체의 용도에 관한 것일 수 있다.In another aspect, the present invention may relate to the use of a human scalp mimetic for measuring scalp cleansing effects.
본 발명은 또 다른 관점에서, 인공 두피 실험용 인간 두피 모사체에 관한 것일 수 있다.In another aspect, the invention may relate to a human scalp mimetic for artificial scalp experiments.
본 발명은 또 다른 관점에서, 두피 세정 효과 측정용 인간 두피 모사체에 관한 것일 수 있다. In another aspect, the present invention may relate to a human scalp mimetic for measuring scalp cleansing effect.
다른 측면에서, 본 발명은, 상기 두피 모사체에 헤어 케어용 물질을 도포하는 단계; 및 도포 후 두피를 세정하는 단계를 포함하는, 두피 세정효과 측정 방법이다. 일 구현예에서, 상기 방법은, 두피 세정 전후의 두피 상의 물질 변화량을 측정하는 단계를 더 포함할 수 있다.In another aspect, the present invention, the step of applying a hair care material to the scalp mimetic; And washing the scalp after application. In one embodiment, the method may further comprise measuring the amount of material change on the scalp before and after scalp cleaning.
본 명세서에서, 세정이란, 두피 오염물질을 두피에서 제거하는 것뿐만 아니라, 두피 오염물질을 제거하기 위하여 사용되는 모발 세정료 조성물도 함께 두피에서 제거하는 것을 의미할 수 있다. 세정은, 물만을 이용하여 할 수 있고, 세정료 조성물을 이용하여 할 수도 있다. 상기 오염물질은, 미세먼지, 비듬균 및 피부 분비물을 포함할 수 있다.In the present specification, the cleaning may mean not only removing the scalp contaminants from the scalp, but also removing the hair cleansing agent composition used to remove the scalp contaminants from the scalp. Cleaning can be performed using only water, and can also be performed using a cleaning material composition. The contaminants may include fine dust, dandruff and skin secretions.
상기와 같은 측면에서, 상기 헤어 케어용 물질은, 모발 세정료 조성물 또는 모발용 화장료 조성물일 수 있다. 상기 헤어 케어용 물질은, 두피 또는 모발의 세정, 영양 공급 또는 미용을 위하여 사용되는 물질들을 통칭하는 것일 수 있다. 예컨대, 상기 모발 세정료 조성물에는, 샴푸 또는 린스가 포함될 수 있고, 상기 모발용 화장료 조성물에는, 헤어토닉, 젤 또는 무스 등의 정발제, 양모제 또는 염모제 등이 포함될 수 있다.In the above aspect, the hair care material may be a hair cleaning composition or hair cosmetic composition. The hair care material may be a generic name for materials used for cleaning, nourishing, or cosmetology of the scalp or hair. For example, the hair cleansing composition may include a shampoo or rinse, and the hair cosmetic composition may include a hair tonic, a hair dressing agent such as a gel or a mousse, a wool or a hair dye, and the like.
이하, 하기의 실시예와 제조예를 통하여 본 발명을 보다 구체적으로 설명한다. 그러나 하기의 실시에는 본 발명에 대한 이해를 돕기 위해 예시의 목적으로만 제공된 것일 뿐, 본 발명의 범주 및 범위가 이에 한정되지 않는다.Hereinafter, the present invention will be described in more detail with reference to the following Examples and Preparation Examples. However, the following practice is provided only for the purpose of illustration in order to help the understanding of the present invention, the scope and scope of the present invention is not limited thereto.
[제조예] 인간 두피 모사체의 제조Preparation Example Preparation of Human Scalp Mimics
우선, 6인치 실리콘 웨이퍼에 맞는 포토마스크(photomask)를 두피 패턴에 따라 제작하였다(도 3). 모발과 모발의 간격은 1000㎛으로 설정하였고 모발의 두께는 80㎛로 하였다.First, a photomask suitable for a 6 inch silicon wafer was prepared according to the scalp pattern (FIG. 3). The spacing between the hair was set to 1000 mu m and the thickness of the hair was 80 mu m.
그 후, 6인치 실리콘 웨이퍼에 감광제(Photoregistive, 제품명 : JSR 151-N 제조사 JSR, 일본)를 모공의 깊이와 동일한 두께로 코팅하였다. 그 후 감광제가 코팅된 실리콘 웨이퍼 위에 포토마스크를 부착시킨 후, UV로 조사하여 패턴의 모발, 모공 부분만 단단히 굳히고, 모발 및 모공 부분의 바깥 부분은 유기용액으로 제거하였다. 그 후, 진공증착장비를 사용하여 금속 또는 무기 코팅물질로 다시 실리콘 웨이퍼 표면을 코팅하고, 감광제는 유기 용액으로 제거하였다. 두피 패턴이 음각으로 새겨져 있는 실리콘 웨이퍼 표면에 용해된 폴리디메틸실록산 용액(제조사; 다우코닝)을 3 ~ 5 mm 두께로 부은 후 상온에서 굳혔다. 굳어진 폴리디메틸실록산을 실리콘 웨이퍼에서 떼어내 최종적으로 두피 모사체를 제작하였다.Thereafter, a photoresist (Photoregistive, JSR 151-N manufacturer JSR, Japan) was coated on the 6-inch silicon wafer to the same thickness as the depth of the pores. Thereafter, the photomask was attached onto the silicon wafer coated with the photosensitive agent, and then irradiated with UV to harden only the hair and the pores of the pattern, and the outer parts of the hair and the pores were removed with an organic solution. Thereafter, the surface of the silicon wafer was again coated with a metal or an inorganic coating material using a vacuum deposition apparatus, and the photoresist was removed with an organic solution. The polydimethylsiloxane solution (manufacturer; Dow Corning) dissolved on the surface of the silicon wafer inscribed with the scalp pattern was engraved and hardened at room temperature. The hardened polydimethylsiloxane was removed from the silicon wafer to finally produce a scalp mimetic.
[실시예] 인간 두피 모사체의 인공 모공과 표면에 잔존하는 물질의 규명EXAMPLES Identification of substances remaining on artificial pores and surfaces of human scalp mimetics
양이온 폴리머, 음이온 폴리머 혼합액(양이온:음이온:정제수 = 0.5:7.5:92) 을 만든 후, 두피 모사체 표면에 올린 다음 5분간 교반시켰다. 사용된 양이온 폴리머는 폴리쿼터늄-10(polyquaternium-10, 시그마알드리치)이고, 음이온 폴리머는 소듐라우레스설페이트(sodium laureth sulfate, 시그마알드리치)였다. 그 후, 상기 혼합액을 버리고, 정제수를 두피 모사체 표면에 올린 후 세척시켰다. 그 후, 인공 모공 내에 잔존하는 코아세르베이트를 공초점 주사 레이져 현미경(Confocal Laser Scanning Microsocpy)을 사용해서 모공 내 형성된 코아세르베이트를 확인하였다(도 4).A cationic polymer and an anionic polymer mixed solution (cation: anion: purified water = 0.5: 7.5: 92) were prepared, and then placed on the scalp replica surface and stirred for 5 minutes. The cationic polymer used was polyquaternium-10 (sigma aldrich) and the anionic polymer was sodium laureth sulfate (sigma aldrich). Thereafter, the mixed solution was discarded, and purified water was placed on the scalp mimic surface and washed. Thereafter, the coacervate remaining in the artificial pores was identified using a confocal laser scanning microscope (Confocal Laser Scanning Microsocpy) to confirm the coacervate formed in the pores (FIG. 4).
[부호의 설명][Description of the code]
10: 고분자 플레이트10: polymer plate
20: 홈(인공 모공)20: home (artificial pores)
21: 홈의 내벽21: the inner wall of the groove
30: 돌출부(인공 모발)30: protrusion (artificial hair)
31: 돌출부 외벽31: protrusion outer wall

Claims (11)

  1. 인간 두피 모사체로서,As a human scalp mimetic,
    표면상에 복수 개의 홈들을 포함하는 고분자 플레이트를 포함하고,A polymer plate comprising a plurality of grooves on a surface thereof,
    상기 복수 개의 홈들은, 인접 홈들의 가장자리 간의 평균 거리가 700㎛ 내지 1500㎛이고, The plurality of grooves have an average distance between edges of adjacent grooves of 700 μm to 1500 μm,
    상기 고분자 플레이트의 탄성은 국제 표준화 기구에 의해 인증된 ISO 37(2011)에 따라 23℃에서 측정하였을 때, 300MPa 이하인, 인간 두피 모사체.The elasticity of the polymer plate is a human scalp mimetic, when measured at 23 ° C according to ISO 37 (2011) certified by the International Organization for Standardization.
  2. 제1항에 있어서,The method of claim 1,
    상기 고분자 플레이트는, 상기 홈의 내벽과 이격된 상태로 홈의 내부에 위치하는 돌출부를 포함하는, 인간 두피 모사체.The polymer plate, human scalp mimetic comprising a protrusion located inside the groove in a state spaced apart from the inner wall of the groove.
  3. 제2항에 있어서,The method of claim 2,
    상기 홈의 내벽과 돌출부의 외벽 사이의 평균 거리는 30㎛ 내지 60㎛인, 인간 두피 모사체.A human scalp mimetic having an average distance between the inner wall of the groove and the outer wall of the protrusion is 30 μm to 60 μm.
  4. 제1항에 있어서,The method of claim 1,
    상기 홈의 깊이는 370㎛ 이하인, 인간 두피 모사체.The depth of the groove is 370㎛ or less, human scalp mimetics.
  5. 제1항에 있어서, The method of claim 1,
    상기 고분자는, 폴리디메틸실록산(polydimethylsiloxane, PDMS), 저밀도 폴리에틸렌(low density polyethylene, LDPE) 또는 폴리비닐클로라이드(polyvinyl chloride)인, 인간 두피 모사체.The polymer is a polydimethylsiloxane (PDMS), low density polyethylene (LDPE) or polyvinyl chloride (polyvinyl chloride), human scalp mimetics.
  6. 제1항에 있어서,The method of claim 1,
    상기 고분자 플레이트의 두께는 1mm 내지 4mm인, 인간 두피 모사체.The thickness of the polymer plate is 1mm to 4mm, human scalp mimetics.
  7. 제1항에 있어서,The method of claim 1,
    상기 고분자 플레이트는,The polymer plate,
    표면상에 수산화기를 포함하는, 인간 두피 모사체.A human scalp mimetic comprising a hydroxyl group on a surface.
  8. 제7항에 있어서,The method of claim 7, wherein
    상기 수산화기는, 플라즈마 에칭법에 의하여 고분자 플레이트 상의 표면상에 부착되는, 인간 두피 모사체.The hydroxyl group is a human scalp mimic attached to the surface on the polymer plate by a plasma etching method.
  9. 제1항 내지 제8항 중 어느 한 항의 두피 모사체에 To the scalp mimetics of any one of claims 1 to 8.
    헤어 케어용 물질을 도포하는 단계; 및Applying a hair care material; And
    도포 후 두피를 세정하는 단계를 포함하는, 두피 세정효과 측정 방법.Method for measuring the scalp cleaning effect, comprising the step of cleaning the scalp after application.
  10. 제9항에 있어서,The method of claim 9,
    두피 세정 전후의 두피 상의 물질 변화량을 측정하는 단계를 더 포함하는, 두피 세정효과 측정 방법.A method for measuring the scalp cleansing effect further comprising the step of measuring the amount of material change on the scalp before and after scalp cleaning.
  11. 제9항에 있어서,The method of claim 9,
    상기 헤어 케어용 물질은,The hair care material,
    모발 세정료 조성물 또는 모발용 화장료 조성물인, 두피 세정효과 측정 방법.A method for measuring the scalp cleaning effect, which is a hair cleaning composition or a cosmetic composition for hair.
PCT/KR2016/010903 2015-09-30 2016-09-29 Human scalp mimic and method for measuring scalp cleansing effect by using same WO2017057917A1 (en)

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KR102372722B1 (en) * 2017-11-20 2022-03-11 (주)아모레퍼시픽 Pore Mimetics, Method of Evaluating Substance Having Skin Cleansing Power Using the Same, and Method of Screening Substance Having Skin Cleansing Power Using the Same

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US5634944A (en) * 1995-02-23 1997-06-03 The Nemours Foundation Body membrane prosthesis
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US5634944A (en) * 1995-02-23 1997-06-03 The Nemours Foundation Body membrane prosthesis
KR100210473B1 (en) * 1995-06-07 1999-07-15 시로 야마다 Man-made hair for hair implanting and process for producing the same
KR19990040321U (en) * 1998-04-30 1999-11-25 이정자 Artificial Scalp for Wig
KR100269270B1 (en) * 1998-07-09 2000-10-16 이임석 Method for the preparation of the wig
JP2012112869A (en) * 2010-11-26 2012-06-14 Mandom Corp Sebum model and method for evaluating washability of detergent for hair

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