WO2017156652A1 - Biological sample detection method based on continuously adjustable biological sample holder - Google Patents
Biological sample detection method based on continuously adjustable biological sample holder Download PDFInfo
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- WO2017156652A1 WO2017156652A1 PCT/CN2016/000610 CN2016000610W WO2017156652A1 WO 2017156652 A1 WO2017156652 A1 WO 2017156652A1 CN 2016000610 W CN2016000610 W CN 2016000610W WO 2017156652 A1 WO2017156652 A1 WO 2017156652A1
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- biological sample
- sample
- terahertz
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- sample holder
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- 239000012472 biological sample Substances 0.000 title claims abstract description 81
- 238000001514 detection method Methods 0.000 title claims abstract description 15
- 229920001903 high density polyethylene Polymers 0.000 claims abstract description 10
- 239000004700 high-density polyethylene Substances 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 4
- 239000000523 sample Substances 0.000 claims description 43
- 239000004698 Polyethylene Substances 0.000 claims description 16
- -1 polyethylene Polymers 0.000 claims description 16
- 229920000573 polyethylene Polymers 0.000 claims description 16
- 238000000862 absorption spectrum Methods 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 238000011109 contamination Methods 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims 1
- 238000012360 testing method Methods 0.000 description 7
- 238000010521 absorption reaction Methods 0.000 description 5
- 230000005855 radiation Effects 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 125000006850 spacer group Chemical group 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3581—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared light; using Terahertz radiation
Definitions
- the present invention relates to a biological sample holder, and more particularly to a method for detecting a suspended, viscous biological sample by using a terahertz wave based on a continuously adjustable biological sample holder.
- THz waves Terahertz radiation, T-rays, sub-millimeter waves, far-infrared rays
- T-rays Terahertz radiation
- T-rays sub-millimeter waves
- far-infrared rays Terahertz radiation
- terahertz waves to detect biological samples is the use of certain substances in biological samples for the non-cooperativeness of terahertz radiation propagation, also known as absorption attenuation. Some specific substances in the sample, the internal atoms of the sample itself will vibrate and rotate. These vibrations and rotations are in the terahertz band, and the terahertz radiation resonates with these vibrations and rotations to obtain many absorption peaks. To achieve the identification of one or more indicators that determine an uncertain biological sample.
- terahertz waves are used to detect fresh biological samples.
- the common method is to use two polyethylene plates with concentric circles of the same size as the support and fix them by a metal ring, while placing a stretchable between the two polyethylene plates.
- a film or plastic gasket (polyethylene, polystyrene, etc.) with high transmittance in the terahertz band constitutes a biological sample detecting device.
- an absorption peak characteristic of the biological sample can be obtained.
- this method also has its limitations: 1. The thickness of the sample is determined by the plastic spacer between the two polyethylene sheets.
- the thickness of the sample is fixed, and the thickness of the sample cannot be adjusted without removing the device. Cumbersome; 2, when the film is filled with the biological sample device, the film is easily deformed, resulting in uneven thickness of the sample; 3, when testing the same biological sample of different thickness, the gasket must be replaced and the biological sample needs to be refilled, the biological sample pole It is easy to be polluted and used in large quantities.
- the invention is directed to the problem of using a terahertz wave to detect a fresh biological sample, and proposes a biological sample detection method based on a continuously adjustable biological sample holder.
- the continuously adjustable biological sample holder is composed of two pieces high. Concentrated polyethylene plate, a high-density and high-elastic round O-ring and four M6 screws, can use a small amount of biological samples, only need to adjust the 4 without disassembling the device, no need to replace the gasket M6 screws to test specific information at different biological sample thicknesses. The whole operation process is simple, convenient and efficient.
- the technical scheme of the invention is: a biological sample detecting method based on a continuously adjustable biological sample rack, wherein the biological sample rack is composed of two high-density polyethylene sheets of the same material size and two high-concentration polyethylene sheets aligned in the middle A high-density, high-elastic circular O-ring and two high-density polyethylene plates with four corners of fixed-adjustment M6 screws, using terahertz waves to detect biological samples filled with the biological sample holder:
- the biological sample holder is disinfected with alcohol to avoid contaminating the biological sample; the empty sample holder is placed in the terahertz detection system, and the terahertz signal of the entire biological sample at no load is measured, and the signal is used as a reference signal;
- the fresh biological sample to be tested is filled in the center circular O-ring, and the biological sample holder is placed in the terahertz detection system after being packaged, and the absorption spectrum signal of the sample is obtained, and four M6 screws are repeatedly adjusted to obtain
- the absorption spectrum of the terahertz wave of the biological sample at different thicknesses is compared with the thickness of the sample when the absorption spectrum signal is most obvious, and the data is collected corresponding to the thickness of the sample for analyzing the characteristic parameters of the sample.
- the invention has the beneficial effects that the invention is based on the biological sample detection method of the continuously adjustable biological sample holder, has a simple structure, and the biological sample thickness can be continuously adjusted, thereby greatly reducing the possibility of contamination of the biological sample.
- FIG. 1 is a schematic view showing the structure of a continuously adjustable biological sample holder according to the present invention
- FIG. 2 is a schematic view showing a biological sample rack in which the thickness of the sample is excessively adjusted during the test of the biological sample of the present invention
- Fig. 3 is a schematic view showing the biological sample holder in which the thickness of the sample is controlled at the most suitable place during the test of the biological sample of the present invention.
- a continuously adjustable biological sample holder structure is characterized in that the surface of the biological sample is uniform in thickness, the required sample is small, and the thickness of the sample can be continuously adjusted, which is very suitable for a small and precious biological sample.
- the biological sample holder is aligned with two high-density polyethylene plates 3 and 4 of the same size and material, a high-density and high-elastic circular O-ring 3 in the middle of the two plates, and a fixed-adjustment M6 screw set with two plates and four corners. to make.
- the high-concentration polyethylene plate transmits terahertz light waves and is opaque to visible light.
- the high-density round O-ring has a certain elasticity. It rotates four M6 screws and squeezes two high-concentration polyethylene plates to change the thickness of the high-density round O-ring containing the biological sample.
- the change in the thickness of the circular O-ring directly drives the change in the thickness of the biological sample.
- the whole device is placed in the terahertz detection system, and the thickness of the sample is repeatedly adjusted to find the position where the sample absorbs the signal with the highest amplitude. At this time, the thickness of the sample is the optimal thickness of the test sample, and the thickness is first measured by the micrometer. The thickness can be obtained by subtracting the thickness of each of the two high-concentration polyethylene sheets.
- a femtosecond wave is radiated by a femtosecond laser having a center wavelength of 800 nm, and a THz-terahertz detection system is used to irradiate a terahertz wave in a dry environment (humidity of 5% or less, temperature of about 20 ° C). The sample is tested.
- the laser output light has a center wavelength of 800 nm, a spectral range of 760-840 nm, a pulse width of 100 fs, and a repetition rate of 78 MHz.
- the biological sample filled with the biological sample holder is detected by the terahertz wave: the terahertz wave generated by the laser through the photoconductive antenna is incident on the first high-purity polyethylene plate, and transmitted through the biological sample, from the second high The purity polyethylene sheet exits to form a transmissive biological sample detection light path.
- the biological sample holder needs to be disinfected with alcohol to avoid contamination of the biological sample; the empty sample holder is placed in the terahertz detection system to measure the terahertz signal of the entire device at no load. And use the signal as a reference signal; then fill the center of the round O-ring with the fresh biological sample to be tested, and then package the whole device in the terahertz detection system to test the absorption spectrum signal of the sample.
- the absorption spectrum of the terahertz wave of the biological sample at different thicknesses is obtained, and the thickness of the sample when the absorption spectrum signal is most obvious is selected, and the data is collected corresponding to the thickness of the sample. Later, it was used to analyze the characteristic parameters of the sample.
- the sample thickness is controlled at the most suitable location for the biological sample holder device.
Abstract
A biological sample detection method based on a continuously adjustable biological sample holder. The biological sample holder consists of two vertically aligned high-density polyethylene sheets (3, 4) having the same size and made of the same material, a high-density high-elasticity circular O-ring (3) in the middle between the two high-density polyethylene sheets, and M6 screws (1) for fastening and adjusting at four corners of the two high-density polyethylene sheets (3, 4). A biological sample with which the biological sample holder is filled is detected by using terahertz waves. Specific information about the biological sample of different thicknesses is tested by adjusting four M6 screws (1). The whole operation process is simple, easy and efficient. The thickness of a biological sample can be continuously adjusted, such that the probability of the biological sample being polluted is greatly reduced.
Description
本发明涉及一种生物样品架，特别涉及一种通过基于连续可调生物样品架用太赫兹波检测悬浊状、粘稠状生物样品的方法。The present invention relates to a biological sample holder, and more particularly to a method for detecting a suspended, viscous biological sample by using a terahertz wave based on a continuously adjustable biological sample holder.
太赫兹(Terahertz，THz)波(或称太赫兹辐射、T-射线、亚毫米波、远红外)因其光子能量低、对生物组织辐射伤害低而备受全世界研究者的关注。其频域范围在0.1THz～10THz(1THz＝1012Hz)，波长在0.03mm～3mm范围。经过诸多研究者多年的不懈努力，曾经的“太赫兹空隙”(THz Gap)已逐渐被填补。Terahertz (THz) waves (or terahertz radiation, T-rays, sub-millimeter waves, far-infrared rays) have attracted the attention of researchers all over the world because of their low photon energy and low radiation damage to biological tissues. The frequency range is from 0.1 THz to 10 THz (1 THz = 10 12 Hz) and the wavelength is in the range of 0.03 mm to 3 mm. After years of unremitting efforts by many researchers, the “THz Gap” has been gradually filled.
利用太赫兹波对生物样品进行检测，就是利用生物样品中某些物质对太赫兹辐射传播存在一定的非协作性，也称之为吸收衰减。样品中的某些特定物质，其内部的原子本身会存在振动和转动，这些振动和转动所在的频段正好是太赫兹波段，通过太赫兹辐射，与这些振动和转动实现共振，从而得到诸多吸收峰，实现找到判定某一不确定生物样品的一个或多个指标。The use of terahertz waves to detect biological samples is the use of certain substances in biological samples for the non-cooperativeness of terahertz radiation propagation, also known as absorption attenuation. Some specific substances in the sample, the internal atoms of the sample itself will vibrate and rotate. These vibrations and rotations are in the terahertz band, and the terahertz radiation resonates with these vibrations and rotations to obtain many absorption peaks. To achieve the identification of one or more indicators that determine an uncertain biological sample.
当前利用太赫兹波检测新鲜生物样品，普遍的方法是将两个中间具有相同尺寸同心圆的聚乙烯板作为支架，并由金属环固定，同时在两个聚乙烯板中间放一个可拉伸且对太赫兹波段具有高透射率的薄膜或者塑料垫片(聚乙烯、聚苯乙烯等)，组成一个生物样品检测装置。在薄膜或塑料垫片处填充生物样品，将整个装置置于太赫兹系统中，就能够得到该生物样品所特有的吸收峰。然而这种方法也有其局限性：1、样品厚度由两块聚乙烯板中间的塑料垫片决定，一旦填充样品，样品厚度就被固定，无法在不拆装置的前提下调节样品厚度，操作过程繁琐；2、选择薄膜填装生物样品装置时，薄膜容易变形，使得样品厚度不均；3、测试不同厚度的同种生物样品时，必须更换垫片且需要重新填装生物样品，生物样品极易被污染，且用量较大。Currently, terahertz waves are used to detect fresh biological samples. The common method is to use two polyethylene plates with concentric circles of the same size as the support and fix them by a metal ring, while placing a stretchable between the two polyethylene plates. A film or plastic gasket (polyethylene, polystyrene, etc.) with high transmittance in the terahertz band constitutes a biological sample detecting device. By filling the biological sample with a film or plastic spacer and placing the entire device in a terahertz system, an absorption peak characteristic of the biological sample can be obtained. However, this method also has its limitations: 1. The thickness of the sample is determined by the plastic spacer between the two polyethylene sheets. Once the sample is filled, the thickness of the sample is fixed, and the thickness of the sample cannot be adjusted without removing the device. Cumbersome; 2, when the film is filled with the biological sample device, the film is easily deformed, resulting in uneven thickness of the sample; 3, when testing the same biological sample of different thickness, the gasket must be replaced and the biological sample needs to be refilled, the biological sample pole It is easy to be polluted and used in large quantities.
发明内容Summary of the invention
本发明是针对利用太赫兹波检测新鲜生物样品的支架存在的问题，提出了一种基于连续可调生物样品架的生物样品检测方法，连续可调生物样品架由两块高
浓度聚乙烯板，一个高密度高弹性的圆形O圈和四个M6螺丝组成，能够利用及少量的生物样品，在不需要拆装置，不需要更换垫片的情况下，仅通过调节4个M6螺丝来测试不同生物样品厚度下的具体信息。整个操作过程简单，方便，工作效率高。The invention is directed to the problem of using a terahertz wave to detect a fresh biological sample, and proposes a biological sample detection method based on a continuously adjustable biological sample holder. The continuously adjustable biological sample holder is composed of two pieces high.
Concentrated polyethylene plate, a high-density and high-elastic round O-ring and four M6 screws, can use a small amount of biological samples, only need to adjust the 4 without disassembling the device, no need to replace the gasket M6 screws to test specific information at different biological sample thicknesses. The whole operation process is simple, convenient and efficient.
本发明的技术方案为：一种基于连续可调生物样品架的生物样品检测方法，生物样品架由上下对齐的2块大小材质相同的高浓度聚乙烯板、2块高浓度聚乙烯板正中间的一个高密度高弹性圆形O圈和2块高浓度聚乙烯板4个角的固定调节M6螺丝组成，利用太赫兹波对采用所述生物样品架填装的生物样品进行检测：The technical scheme of the invention is: a biological sample detecting method based on a continuously adjustable biological sample rack, wherein the biological sample rack is composed of two high-density polyethylene sheets of the same material size and two high-concentration polyethylene sheets aligned in the middle A high-density, high-elastic circular O-ring and two high-density polyethylene plates with four corners of fixed-adjustment M6 screws, using terahertz waves to detect biological samples filled with the biological sample holder:
首先将生物样品架用酒精消毒处理，以免污染生物样品；将空的样品架置于太赫兹检测系统中，测出整个生物样品空载时的太赫兹信号，并把该信号作为参考信号；然后在中心圆形O圈内填充待测新鲜的生物样品，封装好后再将生物样品架置于太赫兹检测系统中，测试得到该样品的吸收光谱信号，重复多次调节4个M6螺丝，获得该生物样品在不同厚度下对太赫兹波的吸收光谱，比较选出吸收光谱信号最明显时的样品厚度，对应此样品厚度采集数据，用于分析该样品的特征参数。First, the biological sample holder is disinfected with alcohol to avoid contaminating the biological sample; the empty sample holder is placed in the terahertz detection system, and the terahertz signal of the entire biological sample at no load is measured, and the signal is used as a reference signal; The fresh biological sample to be tested is filled in the center circular O-ring, and the biological sample holder is placed in the terahertz detection system after being packaged, and the absorption spectrum signal of the sample is obtained, and four M6 screws are repeatedly adjusted to obtain The absorption spectrum of the terahertz wave of the biological sample at different thicknesses is compared with the thickness of the sample when the absorption spectrum signal is most obvious, and the data is collected corresponding to the thickness of the sample for analyzing the characteristic parameters of the sample.
本发明的有益效果在于：本发明基于连续可调生物样品架的生物样品检测方法，结构简单，生物样品厚度可连续调节，极大的降低生物样品被污染的可能性。The invention has the beneficial effects that the invention is based on the biological sample detection method of the continuously adjustable biological sample holder, has a simple structure, and the biological sample thickness can be continuously adjusted, thereby greatly reducing the possibility of contamination of the biological sample.
图1为本发明连续可调的生物样品架结构示意图；1 is a schematic view showing the structure of a continuously adjustable biological sample holder according to the present invention;
图2为本发明测试生物样品过程中，样品厚度调节过度的生物样品架示意图；2 is a schematic view showing a biological sample rack in which the thickness of the sample is excessively adjusted during the test of the biological sample of the present invention;
图3为本发明测试生物样品过程中，样品厚度控制在最合适处的生物样品架示意图。Fig. 3 is a schematic view showing the biological sample holder in which the thickness of the sample is controlled at the most suitable place during the test of the biological sample of the present invention.
如图1所示一种连续可调的生物样品架结构示意，其特点就在于保证生物样品表面平整厚度均匀、所需样品少、样品厚度可连续调节，非常适合量少又珍贵的生物样品。生物样品架由上下对齐2块大小材质相同的高浓度聚乙烯板3、4，两板正中间一个高密度高弹性的圆形O圈3和两板4个角的固定调节M6螺丝组
成。高浓度聚乙烯板对太赫兹光波透过，对可见光不透过。As shown in Fig. 1, a continuously adjustable biological sample holder structure is characterized in that the surface of the biological sample is uniform in thickness, the required sample is small, and the thickness of the sample can be continuously adjusted, which is very suitable for a small and precious biological sample. The biological sample holder is aligned with two high- density polyethylene plates 3 and 4 of the same size and material, a high-density and high-elastic circular O-ring 3 in the middle of the two plates, and a fixed-adjustment M6 screw set with two plates and four corners.
to make. The high-concentration polyethylene plate transmits terahertz light waves and is opaque to visible light.
将高密度圆形O圈3放在第一块高密度聚乙烯板4中心处，然后将新鲜生物样品置于圆形O圈内壁中铺平，盖上第二块高密度聚乙烯板3，让两块聚乙烯板对齐，最后通过4个分散在聚乙烯板4个角的M6螺丝将整个装置固定。Place the high-density round O-ring 3 at the center of the first high-density polyethylene sheet 4, then place the fresh biological sample in the inner wall of the circular O-ring and cover it with a second high-density polyethylene sheet 3, Align the two polyethylene sheets and finally secure the entire unit by four M6 screws scattered over the four corners of the polyethylene sheet.
选用的高密度圆形O圈具有一定的弹性，旋转4个M6螺丝，通过挤压两块高浓度聚乙烯板，从而改变装有生物样品的高密度圆形O圈的厚度。圆形O圈厚度的变化直接带动生物样品厚度的变化。将整个装置置于太赫兹检测系统中，经过反复调节样品厚度，找到样品吸收信号幅度最高的位置，此时样品的厚度即为检测样品的最佳厚度，该厚度通过千分尺先测出整个装置的厚度，再减去两块高浓度聚乙烯板各自的厚度可以得出。The high-density round O-ring has a certain elasticity. It rotates four M6 screws and squeezes two high-concentration polyethylene plates to change the thickness of the high-density round O-ring containing the biological sample. The change in the thickness of the circular O-ring directly drives the change in the thickness of the biological sample. The whole device is placed in the terahertz detection system, and the thickness of the sample is repeatedly adjusted to find the position where the sample absorbs the signal with the highest amplitude. At this time, the thickness of the sample is the optimal thickness of the test sample, and the thickness is first measured by the micrometer. The thickness can be obtained by subtracting the thickness of each of the two high-concentration polyethylene sheets.
在下面的实施例中，以中心波长800nm的飞秒激光器利用光电导天线辐射出太赫兹波，在干燥环境(湿度5％以下，温度20℃左右)下，利用THz-太赫兹检测系统对生物样品进行检测。In the following embodiments, a femtosecond wave is radiated by a femtosecond laser having a center wavelength of 800 nm, and a THz-terahertz detection system is used to irradiate a terahertz wave in a dry environment (humidity of 5% or less, temperature of about 20 ° C). The sample is tested.
激光器输出光中心波长为800nm，光谱范围760-840nm，脉冲宽度为100fs，重复频率78MHz。利用太赫兹波对采用本生物样品架填装的生物样品进行检测：激光经过光电导天线产生的太赫兹波从第一个高纯度聚乙烯板入射后，透射通过生物样品，从第二块高纯度聚乙烯板出射，形成一个透射式的生物样品检测光路。具体过程如下：如图1所示，首先需要将生物样品架用酒精消毒处理，以免污染生物样品；将空的样品架置于太赫兹检测系统中，测出整个装置空载时的太赫兹信号，并把该信号作为参考信号；然后在中心圆形O圈内填充待测新鲜的生物样品，封装好后再将整个装置置于太赫兹检测系统中，测试该样品的吸收光谱信号。通过调节4个M6螺丝来连续调节样品的厚度，从而获得该生物样品在不同厚度下对太赫兹波的吸收光谱，比较选出吸收光谱信号最明显时的样品厚度，对应此样品厚度采集数据，后期用于分析该样品的特征参数。The laser output light has a center wavelength of 800 nm, a spectral range of 760-840 nm, a pulse width of 100 fs, and a repetition rate of 78 MHz. The biological sample filled with the biological sample holder is detected by the terahertz wave: the terahertz wave generated by the laser through the photoconductive antenna is incident on the first high-purity polyethylene plate, and transmitted through the biological sample, from the second high The purity polyethylene sheet exits to form a transmissive biological sample detection light path. The specific process is as follows: As shown in Figure 1, the biological sample holder needs to be disinfected with alcohol to avoid contamination of the biological sample; the empty sample holder is placed in the terahertz detection system to measure the terahertz signal of the entire device at no load. And use the signal as a reference signal; then fill the center of the round O-ring with the fresh biological sample to be tested, and then package the whole device in the terahertz detection system to test the absorption spectrum signal of the sample. By adjusting the thickness of the sample by adjusting 4 M6 screws, the absorption spectrum of the terahertz wave of the biological sample at different thicknesses is obtained, and the thickness of the sample when the absorption spectrum signal is most obvious is selected, and the data is collected corresponding to the thickness of the sample. Later, it was used to analyze the characteristic parameters of the sample.
在图2中，调节螺丝，通过高浓度聚乙烯板挤压圆形O圈，改变生物样品的厚度。当拧紧4个M6螺丝后，O圈在聚乙烯板和生物样品的双重挤压下，O圈整体变扁且内部样品填装区域面积变大，厚度变薄；反之，拧松螺丝，O圈在自身弹力的作用下趋于恢复原样，生物样品在O圈的带动下，内部样品面积缩小，趋于恢复原有厚度，所以通过调节螺丝，能够很好地实现控制样品厚度的效果。通过此种方法，经过多次测试，我们能够很好地找到最适合检测某一生物样
品时的最佳厚度。图2中所示即为调节螺丝后，螺丝拧的过紧，导致生物样品厚度偏薄，太赫兹检测系统测出来的样品信号过低的情况。In Figure 2, adjust the screw and squeeze the round O-ring through a high-concentration polyethylene plate to change the thickness of the biological sample. When the four M6 screws are tightened, the O-ring is double-squeezed under the polyethylene plate and the biological sample, the O-ring is flattened as a whole and the area of the internal sample filling area becomes larger and the thickness becomes thinner; otherwise, the screw is loosened, the O-ring Under the action of its own elastic force, it tends to return to the original state. Under the driving of the O-ring, the internal sample area shrinks and tends to restore the original thickness. Therefore, by adjusting the screw, the effect of controlling the thickness of the sample can be well achieved. Through this method, after many tests, we can find the best one to detect a certain biological sample.
The optimum thickness of the product. As shown in Fig. 2, after the adjusting screw, the screw is tightened too much, resulting in a thin thickness of the biological sample, and the sample signal measured by the terahertz detection system is too low.
经过反复调节螺丝的位置，我们发现，在某一位置处，无论样品变厚或是变薄，样品对太赫兹波的吸收幅值都在缩小。该现象表明，此时的O圈厚度(即为样品厚度)为检测该样品的最佳厚度，同时在检测该样品时，选用此厚度的样品进行检测，太赫兹吸收信号最强，样品信息更加丰富。如图3所示，样品厚度控制在最合适处的生物样品架装置示意图。
After repeatedly adjusting the position of the screw, we found that at a certain position, regardless of whether the sample becomes thick or thin, the absorption amplitude of the sample to the terahertz wave is reduced. This phenomenon indicates that the thickness of the O-ring at this time (that is, the thickness of the sample) is the optimum thickness for detecting the sample, and when the sample is detected, the sample of this thickness is selected for detection, and the terahertz absorption signal is the strongest, and the sample information is more rich. As shown in Figure 3, the sample thickness is controlled at the most suitable location for the biological sample holder device.
Claims (1)
- 一种基于连续可调生物样品架的生物样品检测方法，其特征在于，生物样品架由上下对齐的2块大小材质相同的高浓度聚乙烯板、2块高浓度聚乙烯板正中间的一个高密度高弹性圆形O圈和2块高浓度聚乙烯板4个角的固定调节M6螺丝组成，利用太赫兹波对采用所述生物样品架填装的生物样品进行检测：首先将生物样品架用酒精消毒处理，以免污染生物样品；将空的样品架置于太赫兹检测系统中，测出整个生物样品空载时的太赫兹信号，并把该信号作为参考信号；然后在中心圆形O圈内填充待测新鲜的生物样品，封装好后再将生物样品架置于太赫兹检测系统中，测试得到该样品的吸收光谱信号，重复多次调节4个M6螺丝，获得该生物样品在不同厚度下对太赫兹波的吸收光谱，比较选出吸收光谱信号最明显时的样品厚度，对应此样品厚度采集数据，用于分析该样品的特征参数。 The invention relates to a biological sample detecting method based on a continuously adjustable biological sample rack, which is characterized in that: the biological sample rack is composed of two high-density polyethylene sheets of the same size and the same material, and two high-concentration polyethylene sheets are in the middle of the middle. The high-density elastic circular O-ring and the two high-density polyethylene plates are composed of four fixed-adjustment M6 screws. The biological samples filled with the biological sample holder are detected by terahertz waves: firstly, the biological sample holder is used. Alcohol disinfection to avoid contamination of biological samples; placing an empty sample holder in a terahertz detection system, measuring the terahertz signal of the entire biological sample at no load, and using the signal as a reference signal; The fresh biological sample to be tested is filled, and after the package is completed, the biological sample holder is placed in the terahertz detection system, and the absorption spectrum signal of the sample is obtained, and four M6 screws are repeatedly adjusted to obtain the biological sample at different thicknesses. The absorption spectrum of the terahertz wave is compared, and the thickness of the sample with the most obvious absorption spectrum signal is selected, and the data is collected corresponding to the thickness of the sample for analysis. Product characteristic parameters.
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