The present invention is based on the discovery that a high titer reassortant influenza virus is produced in mammalian cell culture by replacing the NS gene of the A/PuertoRico/3/24 master strain with the NS gene of the A/England/1/53 strain. The invention provides influenza viruses and vaccines generated...http://www.google.es/patents/US7037707?utm_source=gb-gplus-sharePatente US7037707 - Method for generating influenza viruses and vaccines
Method for generating influenza viruses and vaccines