A method for transferring a long-chain DNA into protoplasts, comprising the steps of: (a) mixing a suspension of protoplasts, polyethylene glycol, and a long-chain DNA to give a final concentration of the DNA of at least 50 .mu.g/ml; (b) collecting, washing, and culturing the protoplasts in a medium;...http://www.google.es/patents/US6143949?utm_source=gb-gplus-sharePatente US6143949 - Method for transferring gene
