This invention is directed to cryopreservation of cultured tissue equivalents made by in vitro technology. The invention involves immersing a cultured tissue equivalent in a cooled cryoprotectant solution, agitating the cryoprotectant solution and the immersed cultured tissue to achieve effective penetration...http://www.google.es/patents/US5518878?utm_source=gb-gplus-sharePatente US5518878 - Cryopreservation of cultured skin or cornea equivalents with agitation
Cryopreservation of cultured skin or cornea equivalents with agitation