An improved method for performing a nested polymerase chain reaction (PCR) amplification of a targeted piece of DNA, wherein by controlling the annealing times and concentration of both the outer and the inner set of primers according to the method disclosed, highly specific and efficient amplification...http://www.google.es/patents/US5340728?utm_source=gb-gplus-sharePatente US5340728 - Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
Method for amplification of targeted segments of nucleic acid using nested ...