Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for...http://www.google.es/patents/US5561058?utm_source=gb-gplus-sharePatente US5561058 - Methods for coupled high temperatures reverse transcription and polymerase chain reactions
Methods for coupled high temperatures reverse transcription and polymerase ...
