Method for synthesizing or sequencing a nucleic acid molecule in a thermocycling reaction which initially comprises a nucleic acid molecule, a first primer, a second primer, a reaction buffer, a first thermostable enzyme e.g. a DNA polymerase, (optionally) a thermostable pyrophosphatase, deoxynucleotides...http://www.google.es/patents/US6399304?utm_source=gb-gplus-sharePatente US6399304 - Sequential activation of one or more enzymatic activities within a thermocycling reaction for synthesizing DNA molecules
Sequential activation of one or more enzymatic activities within a ...