Albumin fusion proteins

US 20070027306A1

(io) Pub. No.: US 2007/0027306 Al (43) Pub. Date: Feb. 1, 2007

(54) ALBUMIN FUSION PROTEINS

Publication Classification

(76) Inventors: Craig A. Rosen, Laytonsville, MD
(US); William A. Haseltine,
Washington, DC (US); Steven M.
Ruben, Brookeville, MD (US)

Correspondence Address:

FINNEGAN, HENDERSON, FARABOW,

GARRETT & DUNNER

LLP

901 NEW YORK AVENUE, NW
WASHINGTON, DC 20001-4413 (US)

(21) Appl. No.: 11/500,508

(22) Filed: Aug. 8, 2006

Related U.S. Application Data

(63) Continuation-in-part of application No. PCT/US05/ 04041, filed on Feb. 9, 2005.

(60) Provisional application No. 60/542,274, filed on Feb. 9, 2004. Provisional application No. 60/549,901, filed on Mar. 5, 2004. Provisional application No. 60/556, 906, filed on Mar. 29, 2004. Provisional application No. 60/636,603, filed on Dec. 17, 2004.

1 GAT GCA CAC AAG AGT GAG GTT GCT CAT CGG ID A H K S E V A H R

61 GCC TTG GTG TTG ATT GCC TTT GCT CAG TAT 21ALVLIAFAQY

181 AAT TGT GAC AAA TCA CTT CAT ACC CTT TTT 61NCDKSLHTLF

241 CGT GAA ACC TAT GGT GAA ATG GCT GAC TGC 81 R E T Y G E M A D C

301 TGC TTC TTG CAA CAC AAA GAT GAC AAC CCA
101 C F L Q H K D D N P

361 GAT GTG ATG TGC ACT GCT TTT CAT GAC AAT
121 D VMCTAFHDN

421 GAA ATT GCC AGA AGA CAT CCT TAC TTT TAT
141 EIARRHPY FY

TTT AAA GAT TTG GGA GAA GAA AAT TTC AAA 60 FKDLGEENFK20

CTT CAG CAG TGT CCA TTT GAA GAT CAT GTA 12 0 LQQCPFEDHV40

GGA GAC AAA TTA TGC ACA GTT GCA ACT CTT 240
GDKLCTVATL80

TGT GCA AAA CAA GAA CCT GAG AGA AAT GAA 300
CAKQEPERNE 100

AAC CTC CCC CGA TTG GTG AGA CCA GAG GTT 360
NLPRLVRPEV 120

GAA GAG ACA TTT TTG AAA AAA TAC TTA TAT 420
E E T F L K K Y L Y 140

GCC CCG GAA CTC CTT TTC TTT GCT AAA AGG 480
APELLFFAKR 160

481 TAT AAA GCT GCT TTT ACA GAA TGT TGC CAA GCT GCT GAT AAA GCT GCC TGC CTG TTG CCA 540 161 YKAAFTECCQAADKAACLLP 180

541 AAG CTC GAT GAA CTT CGG GAT GAA GGG AAG GCT TCG TCT GCC AAA CAG AGA CTC AAA TGT 600 181 KLDELRDEGKASSAKQRLKC 200

601 GCC AGT CTC CAA AAA TTT GGA GAA AGA GCT TTC AAA GCA TGG GCA GTG GCT CGC CTG AGC 660 201 ASLQKFGERAFKAWAVARLS 220

661 CAG AGA TTT CCC AAA GCT GAG TTT GCA GAA GTT TCC AAG TTA GTG ACA GAT CTT ACC AAA 720 221 QRFPKAEFAEVSKLVTDL TK 240

721 GTC CAC ACG GAA TGC TGC CAT GGA GAT CTG CTT GAA TGT GCT GAT GAC AGG GCG GAC CTT 780 241 VHTECCHGDLLECADDRA DL 260

781 GCC AAG TAT ATC TGT GAA AAT CAG GAT TCG ATC TCC AGT AAA CTG AAG GAA TGC TGT GAA 840 261 AKYICENQDS ISSKLKECCE 280

841 AAA CCT CTG TTG GAA AAA TCC CAC TGC ATT GCC GAA GTG GAA AAT GAT GAG ATG CCT GCT 900 281 KPLLEKSHCI A EVENDEMPA 300

901 GAC TTG CCT TCA TTA GCT GCT GAT TTT GTT GAA AGT AAG GAT GTT TGC AAA AAC TAT GCT 960 301 D L P S L A A D F V E S K D V C K N Y A 320

Figure 1B