[54] TEST STRIP
[75] Inventors: Ralph P. McCroskey, Carmel, Ind.;
Helmut E. C. Freitag, Weinheim,
Fed. Rep. of Germany; Mary C.
Smith, Indianapolis; Kenneth J. Dean,
Carmel; Stephanie Secrest,
Bloomington; Lee Bouse, Inopls, all
of Ind.
[73] Assignee: Boehringer Mannheim Corporation,
Indianapolis, Ind.
[21] Appl. No.: 33,451
[22] Filed: Mar. 18, 1993
Related U.S. Application Data
[63] Continuation of Ser. No. 661,788. Feb. 27, 1991, abandoned.
[51] Int. CI.5 C07N 21/00; C12M 1/34
[52] U.S. CI 422/58; 422/61;
422/57; 422/56; 422/101: 435/970: 435/291
[58] Field of Search 422/56, 57, 58, 60.
422/61. 101: 436/169. 170: 435/7.92. 7.94. 291,
970, 975
[56] References Cited
U.S. PATENT DOCUMENTS
4.857.273 8/1989 Stewart 422/68.1
4.876.067 10/1989 Deneke et al 422/56
[ii] Patent Number: 5,271,895 [45] Date of Patent: Dec. 21, 1993
4,956,302 9/1990 Gordon et al 436/161
4,981,786 1/1991 Dafforn et al 435/7
5,037,736 8/1991 Freitag et al 435/7.9
5,053,197 10/1991 Bowen 422/58
5,059,394 10/1991 Phillips et al 422/68.1
5,114,673 5/1992 Berger et al 422/56
FOREIGN PATENT DOCUMENTS
0271854 6/1988 European Pat. Off. 435/970
Primary Examiner—Robert J. Warden
Assistant Examiner—T. A. Trembley
Attorney, Agent, or Firm—Max J. Kenemore; D.
Michael Young; Marilyn L. Amick
[57] ABSTRACT
The operative site of an assay device is dosed with a controlled volume of a liquid specimen by a holding device which holds the controlled volume of specimen in contact with the operative site, although the holding device remains out of contact with the operative site. A sink device is in contact with the margin of the holding device and moves excess specimen away from the holding device. In one embodiment the device makes possible a non-wipe teststrip architecture. The device can be used in the detection of analytes including glucose or cholesterol in blood or urine and can be interrogated visually, optically or electrically, depending on the reagent used at the operative site.
14 Claims, 3 Drawing Sheets
