(i9) United States
(12) Patent Application Publication oo) Pub. No.: US 2006/0257884 Al
Brawley et al. (43) Pub. Date: Nov. 16,2006
(54) METHODS FOR PREPARING AND ANALYZING CELLS HAVING CHROMOSOMAL ABNORMALITIES
(75) Inventors: James Brawley, Seattle, WA (US);
Philip J. Morrissey, Seattle, WA (US);
Rosalynde J. Finch, Seattle, WA (US);
David A. Basiji, Seattle, WA (US);
Luchuan Liang, Woodinville, WA (US)
SEED INTELLECTUAL PROPERTY LAW
701 FIFTH AVE
SEATTLE, WA 98104-7092 (US) (73) Assignee: AMNIS CORPORATION, Seattle, WA
(21) Appl. No.: 11/134,243
(22) Filed: May 20, 2005
Related U.S. Application Data
(60) Provisional application No. 60/573,775, filed on May 20, 2004.
(51) Int. CI.
C12Q 1/68 (2006.01)
(52) U.S. CI 435/6
The present invention provides methods for preparing cells with highly condensed chromosomes, such as sperm, and methods for detecting and quantifying specific cellular target molecules in intact cells. Specifically, methods are provided for detecting chromosomes and chromosomal abnormalities, including aneuploidy, in intact cells using fluorescence in situ hybridization of cells in suspension, such as sperm cells.